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Pathogenicity and Antibiotic Resistance of Helicobacter pylori

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 31 January 2025 | Viewed by 1023

Special Issue Editor


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Guest Editor
Department of Medical Microbiology, Wroclaw Medical University, 50-368 Wroclaw, Poland
Interests: Helicobacter pylori; biofilm; coccoid forms; morphology; outer membrane vesicles; antibiotic tolerance; antimicrobial/antibiofilm activity
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Special Issue Information

Dear Colleagues,

Helicobacter pylori is one of the most common human pathogens. Even though more than 40 years have passed since its discovery, knowledge about methods aimed at effective control of this bacterium is still insufficient. The reason for this impasse is strictly linked to a wide range of virulence factors enabling this pathogen for the establishment of a chronic infection and its easiness of acquiring mechanisms of antibiotic resistance/tolerance.

Therefore, the main goal of this Special Issue is to encourage scientists and clinicians to share the latest scientific reports on the pathogenicity and antibiotic resistance of Helicobacter pylori. As part of the Special Issue, authors are encouraged to submit original research papers, short communications and reviews describing the molecular phenomena responsible for the pathogenesis of this bacterium (adhesins, toxins, enzymes), as well as the development of both antibiotic resistance (point mutations) and antibiotic tolerance (efflux pumps, biofilm formation, transition into coccoid forms and secretion of membrane vesicles).

Dr. Paweł Krzyżek
Guest Editor

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Keywords

  • Helicobacter pylori
  • antibiotic resistance
  • antibiotic tolerance
  • point mutations
  • efflux pumps
  • biofilm formation
  • coccoid forms
  • membrane vesicles
  • virulence factors

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Published Papers (1 paper)

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Research

18 pages, 18776 KiB  
Article
Optimization of Helicobacter pylori Biofilm Formation in In Vitro Conditions Mimicking Stomach
by Paweł Krzyżek, Paweł Migdał, Barbara Krzyżanowska and Anna Duda-Madej
Int. J. Mol. Sci. 2024, 25(18), 9839; https://doi.org/10.3390/ijms25189839 - 11 Sep 2024
Viewed by 749
Abstract
Helicobacter pylori is one of the most common bacterial pathogens worldwide and the main etiological agent of numerous gastric diseases. The frequency of multidrug resistance of H. pylori is growing and the leading factor related to this phenomenon is its ability to form [...] Read more.
Helicobacter pylori is one of the most common bacterial pathogens worldwide and the main etiological agent of numerous gastric diseases. The frequency of multidrug resistance of H. pylori is growing and the leading factor related to this phenomenon is its ability to form biofilm. Therefore, the establishment of a proper model to study this structure is of critical need. In response to this, the aim of this original article is to validate conditions of the optimal biofilm development of H. pylori in monoculture and co-culture with a gastric cell line in media simulating human fluids. Using a set of culture-based and microscopic techniques, we proved that simulated transcellular fluid and simulated gastric fluid, when applied in appropriate concentrations, stimulate autoaggregation and biofilm formation of H. pylori. Additionally, using a co-culture system on semi-permeable membranes in media imitating the stomach environment, we were able to obtain a monolayer of a gastric cell line with H. pylori biofilm on its surface. We believe that the current model for H. pylori biofilm formation in monoculture and co-culture with gastric cells in media containing host-mimicking fluids will constitute a platform for the intensification of research on H. pylori biofilms in in vitro conditions that simulate the human body. Full article
(This article belongs to the Special Issue Pathogenicity and Antibiotic Resistance of Helicobacter pylori)
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