Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
8.1
4.9
Journals
IJMS
Special Issues
Bioprocesses and Biotechnology Related to Extremophiles—Molecular Aspects
ijms-logo
Submit to IJMS Review for IJMS Propose a Special Issue

Journal Menu

► Journal Menu

Journal Browser

► Journal Browser
share announcement

Bioprocesses and Biotechnology Related to Extremophiles—Molecular Aspects

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: closed (20 September 2024) | Viewed by 4877

Special Issue Editors

Dr. Martina Aulitto

E-Mail Website
Guest Editor
Department of Biology, Università degli Studi di Napoli Federico II, 80138 Napoli, Italy
Interests: thermophilic microorganisms; microbial consortia; biopolymers; microbial bioprocesses; microbial cell factories; genomic and omic analyses
Special Issues, Collections and Topics in MDPI journals
Dr. Paola Imbimbo

E-Mail Website
Guest Editor
Department of Chemical Sciences, University of Naples Federico II, Via Cinthia 4, 80126 Naples, Italy
Interests: microalgae biorefinery; high-value products; antioxidants

Special Issue Information

Dear Colleagues,

Over the last decades, the number of isolated and sequenced extremophiles has drastically increased. Since extremophiles thrive in harsh environments, the study of their metabolic pathways and enzymes has gained interest owing to their ability to catalyze reactions under severe industrial conditions. Currently, extremozymes from thermophilic, psychrophilic alkaliphilic, acidophilic, and halophilic microorganisms are employed in several biotechnological processes. These cell biocatalysts or their enzymes are applied in different fields such as wastewater treatment, resource recovery, bioremediation, and for the production of biofuels and biochemicals. To date, metabolic engineering and synthetic biology technologies for high-efficiency bioprocess applications are also introduced.

This Special Issue is supervised by Dr. Martina Aulitto (Guest Editor) and Dr. Paola Imbimbo (Co-Guest Editor) and assisted by our Topical Advisory Panel Member Dr. Giovanni Gallo (Ludwig-Maximilians University Munich). The aim is to collect original research articles, review articles, and short communications dealing with the comprehensive application of extremophiles and extremozymes in biotechnological bioprocesses.

Dr. Martina Aulitto
Dr. Paola Imbimbo
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • waste circularity
  • plastic recycle
  • lignocellulose
  • extremozymes
  • microbial ecology
  • microbial bioprocess

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (4 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

Jump to: Review

19 pages, 4764 KiB  
Article
Genomics, Proteomics, and Antifungal Activity of Chitinase from the Antarctic Marine Bacterium Curtobacterium sp. CBMAI 2942
by Yesenia Melissa Santa-Cruz Vasquez, Luis Gabriel Cueva-Yesquen, Alysson Wagner Fernandes Duarte, Luiz Henrique Rosa, Rodrigo Valladão, Adriana Rios Lopes, Rafaella Costa Bonugli-Santos and Valéria Maia de Oliveira
Int. J. Mol. Sci. 2024, 25(17), 9250; https://doi.org/10.3390/ijms25179250 - 26 Aug 2024
Viewed by 751
Abstract
This study aimed to evaluate the genomic profile of the Antarctic marine Curtobacterium sp. CBMAI 2942, as well as to optimize the conditions for chitinase production and antifungal potential for biological control. Assembly and annotation of the genome confirmed the genomic potential for [...] Read more.
This study aimed to evaluate the genomic profile of the Antarctic marine Curtobacterium sp. CBMAI 2942, as well as to optimize the conditions for chitinase production and antifungal potential for biological control. Assembly and annotation of the genome confirmed the genomic potential for chitinase synthesis, revealing two ChBDs of chitin binding (Chi C). The optimization enzyme production using an experimental design resulted in a 3.7-fold increase in chitinase production. The chitinase enzyme was identified by SDS-PAGE and confirmed through mass spectrometry analysis. The enzymatic extract obtained using acetone showed antifungal activity against the phytopathogenic fungus Aspergillus sp. series Nigri CBMAI 1846. The genetic capability of Curtobacterium sp. CBMAI 2942 for chitin degradation was confirmed through genomic analysis. The basal culture medium was adjusted, and the chitinase produced by this isolate from Antarctica showed significant inhibition against Aspergillus sp. Nigri series CBMAI 1846, which is a tomato phytopathogenic fungus. This suggests that this marine bacterium could potentially be used as a biological control of agricultural pests. Full article
(This article belongs to the Special Issue Bioprocesses and Biotechnology Related to Extremophiles—Molecular Aspects)
►▼ Show Figures

Figure 1

17 pages, 2768 KiB  
Article
Transcriptome Analysis Reveals the Important Role of Vitamin B12 in the Response of Natronorubrum daqingense to Salt Stress
by Qi Wang, Zhiwei Wang, Jiaqi Guan and Jinzhu Song
Int. J. Mol. Sci. 2024, 25(8), 4168; https://doi.org/10.3390/ijms25084168 - 10 Apr 2024
Viewed by 948
Abstract
Natronorubrum daqingense JX313T is an extremely halophilic archaea that can grow in a NaCl-saturated environment. The excellent salt tolerance of N. daqingense makes it a high-potential candidate for researching the salt stress mechanisms of halophilic microorganisms from Natronorubrum. In this study, [...] Read more.
Natronorubrum daqingense JX313T is an extremely halophilic archaea that can grow in a NaCl-saturated environment. The excellent salt tolerance of N. daqingense makes it a high-potential candidate for researching the salt stress mechanisms of halophilic microorganisms from Natronorubrum. In this study, transcriptome analysis revealed that three genes related to the biosynthesis of vitamin B12 were upregulated in response to salt stress. For the wild-type (WT) strain JX313T, the low-salt adaptive mutant LND5, and the vitamin B12 synthesis-deficient strain ΔcobC, the exogenous addition of 10 mg/L of vitamin B12 could maximize their cell survival and biomass in both optimal and salt stress environments. Knockout of cobC resulted in changes in the growth boundary of the strain, as well as a significant decrease in cell survival and biomass, and the inability to synthesize vitamin B12. According to the HPLC analysis, when the external NaCl concentration (w/v) increased from 17.5% (optimal) to 22.5% (5% salt stress), the intracellular accumulation of vitamin B12 in WT increased significantly from (11.54 ± 0.44) mg/L to (15.23 ± 0.20) mg/L. In summary, N. daqingense is capable of absorbing or synthesizing vitamin B12 in response to salt stress, suggesting that vitamin B12 serves as a specific compatible solute effector for N. daqingense during salt stress. Full article
(This article belongs to the Special Issue Bioprocesses and Biotechnology Related to Extremophiles—Molecular Aspects)
►▼ Show Figures

Figure 1

16 pages, 3239 KiB  
Article
Characterization of Two Na+(K+, Li+)/H+ Antiporters from Natronorubrum daqingense
by Qi Wang, Mengwei Qiao and Jinzhu Song
Int. J. Mol. Sci. 2023, 24(13), 10786; https://doi.org/10.3390/ijms241310786 - 28 Jun 2023
Cited by 2 | Viewed by 1292
Abstract
The Na+/H+ antiporter NhaC family protein is a kind of Na+/H+ exchanger from the ion transporter (IT) superfamily, which has mainly been identified in the halophilic bacteria of Bacillus. However, little is known about the Na [...] Read more.
The Na+/H+ antiporter NhaC family protein is a kind of Na+/H+ exchanger from the ion transporter (IT) superfamily, which has mainly been identified in the halophilic bacteria of Bacillus. However, little is known about the Na+/H+ antiporter NhaC family of proteins in the extremely halophilic archaea. In this study, two Na+/H+ antiporter genes, nhaC1 and nhaC2, were screened from the genome of Natronorubrum daqingense based on the gene library and complementation of salt-sensitive Escherichia coli KNabc. A clone vector pUC18 containing nhaC1 or nhaC2 could make KNabc tolerate 0.6 M/0.7 M NaCl or 30 mM/40 mM LiCl and a pH of up to 8.5/9.5, respectively. Functional analysis shows that the Na+(K+, Li+)/H+ antiport activities of NhaC1 and NhaC2 are both pH-dependent in the range of pH 7.0–10.0, and the optimal pH is 9.5. Phylogenetic analysis shows that both NhaC1 and NhaC2 belong to the Na+/H+ antiporter NhaC family of proteins and are significantly distant from the identified NhaC proteins from Bacillus. In summary, we have identified two Na+(K+, Li+)/H+ antiporters from N. daqingense. Full article
(This article belongs to the Special Issue Bioprocesses and Biotechnology Related to Extremophiles—Molecular Aspects)
►▼ Show Figures

Figure 1

Review

Jump to: Research

16 pages, 884 KiB  
Review
The Undeniable Potential of Thermophiles in Industrial Processes
by Giovanni Gallo, Paola Imbimbo and Martina Aulitto
Int. J. Mol. Sci. 2024, 25(14), 7685; https://doi.org/10.3390/ijms25147685 - 13 Jul 2024
Cited by 1 | Viewed by 1326
Abstract
Extremophilic microorganisms play a key role in understanding how life on Earth originated and evolved over centuries. Their ability to thrive in harsh environments relies on a plethora of mechanisms developed to survive at extreme temperatures, pressures, salinity, and pH values. From a [...] Read more.
Extremophilic microorganisms play a key role in understanding how life on Earth originated and evolved over centuries. Their ability to thrive in harsh environments relies on a plethora of mechanisms developed to survive at extreme temperatures, pressures, salinity, and pH values. From a biotechnological point of view, thermophiles are considered a robust tool for synthetic biology as well as a reliable starting material for the development of sustainable bioprocesses. This review discusses the current progress in the biomanufacturing of high-added bioproducts from thermophilic microorganisms and their industrial applications. Full article
(This article belongs to the Special Issue Bioprocesses and Biotechnology Related to Extremophiles—Molecular Aspects)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-4
Back to TopTop