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Molecular Advances in Analytical Techniques for Biological and Medical Research: 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 31 March 2025 | Viewed by 1206

Special Issue Editors


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Guest Editor
Department of Sciences, University of Basilicata, Via dell'Ateneo Lucano, 10-85100 Potenza, Italy
Interests: metabolomics; proteomics; mass spectrometry; liquid chromatography; analytical chemistry
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Department of Basic and Applied Sciences, University of Basilicata, Viale dell’Ateneo Lucano, 10-85100 Potenza, Italy
Interests: liquid chromatography; mass spectrometry; high-resolution mass spectrometry; food; metabolomics; environment
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is a continuation of our previous Special Issue, titled "Molecular Advances in Analytical Techniques for Biological and Medical Research".

The development and validation of new analytical and bioanalytical methods play a critical role in the field of biological and medical research. The most common challenges that arise during method development are the use of an appropriate sample pretreatment and the utilization of up-to-date techniques for the identification, separation, and final determination of the most suitable biomarkers from different matrices on the basis of their physicochemical properties. Therefore, it is our pleasure to invite authors and members of their research groups to submit an article for a Special Issue of the International Journal of Molecular Science, titled “Molecular Advances in Analytical Techniques for Biological and Medical Research: 2nd Edition”.

The present Special Issue will include reviews and full-length research articles focusing on the most recent analytical advances in the field of biological and medical research and their applications in several areas. This Special Issue is aimed at providing selected contributions on analytical method development, optimization, and validation, as well as on the applications of analytical tools for biological and medical research. Potential topics include, but are not limited to, HPLC, GC, electrophoresis, mass spectrometry, plant materials, validation, metabolomics, proteomics, and protein–ligand interaction. All manuscripts will undergo comprehensive reviews according to standard journal procedures and policies. Thank you for considering our invitation.

Dr. Angela Di Capua
Dr. Maria Assunta Acquavia
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • HPLC
  • GC
  • electrophoresis
  • mass spectrometry
  • plant materials
  • validation
  • metabolomics
  • proteomics
  • protein–ligand interaction.

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Published Papers (1 paper)

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Research

14 pages, 9343 KiB  
Article
Accumulation of Bioactive Lipid Species in LPS-Induced Neuroinflammation Models Analysed with Multi-Modal Mass Spectrometry Imaging
by Irma Berrueta Razo, Kerry Shea, Tiffany-Jayne Allen, Hervé Boutin, Adam McMahon, Nicholas Lockyer and Philippa J. Hart
Int. J. Mol. Sci. 2024, 25(22), 12032; https://doi.org/10.3390/ijms252212032 - 8 Nov 2024
Viewed by 945
Abstract
Neuroinflammation is a complex biological process related to a variety of pathologies, often requiring better understanding in order to develop new, targeted therapeutic interventions. Within this context, multimodal Mass Spectrometry Imaging (MSI) has been used to characterise molecular changes in neuroinflammation for biomarker [...] Read more.
Neuroinflammation is a complex biological process related to a variety of pathologies, often requiring better understanding in order to develop new, targeted therapeutic interventions. Within this context, multimodal Mass Spectrometry Imaging (MSI) has been used to characterise molecular changes in neuroinflammation for biomarker discovery not possible to other techniques. In this study, molecules including bioactive lipids were detected across inflamed regions of the brain in rats treated with lipopolysaccharide (LPS). The detected lipids may be acting as inflammatory mediators of the immune response. We identified that N-acyl-phosphatidylethanolamine (NAPE) species accumulated in the inflamed area. The presence of these lipids could be related to the endocannabinoid (eCB) signalling system, mediating an anti-inflammatory response from microglial cells at the site of injury to balance pro-inflammation and support neuronal protection. In addition, polyunsaturated fatty acids (PUFAs), specifically n-3 and n-6 species, were observed to accumulate in the area where LPS was injected. PUFAs are directly linked to anti-inflammatory mediators resolving inflammation. Finally, acylcarnitine species accumulated around the inflammation region. Accumulation of these molecules could be due to a deficient β-oxidation cycle. Full article
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