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Ovarian Stem Cells

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: closed (31 August 2022) | Viewed by 3201

Special Issue Editor


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Guest Editor
Biomedical Research Institute, Seoul National University Hospital, 101 Daehak-ro, Jongro-gu, Seoul 03080, Korea
Interests: stem cell biology; stem cell therapy; reproductive biology; reproductive engineering
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Special Issue Information

Dear Colleagues,

In recent years, new therapeutic approaches are in high demand due to the increases in clinical symptoms which could be solved by a new source of female germ cells. The question of de novo synthesis of oocytes and the ability of pluripotent stem cells to differentiate into germ cells triggers the concept of “ovarian stem cells”.

The goal of this Special Issue is the communication of stem cell technology and concept of ovarian stem cells to new scientific disciplines regarding female germ cell generation.

The Special Issue includes various approaches to isolation and culture of female germ cells, stem cell differentiation into gametes, biomaterials for generation of female germ cells and tissue engineering for the generation of germ cells, in vivo models and therapeutic approach using stem cell-derived germ cells.

Original articles and reviews will be welcome for submission, and the fast peer-review process will enhance the impact of submitted articles.

Dr. Yoon Young Kim
Guest Editor

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Keywords

  • ovarian stem cells
  • female germ cells
  • stem cell differentiation
  • oocytes
  • stem cell therapy
  • reproductive biology
  • reproductive engineering

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Published Papers (1 paper)

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Research

17 pages, 5834 KiB  
Article
Epigenomic Profiling of Epithelial Ovarian Cancer Stem-Cell Differentiation Reveals GPD1 Associated Immune Suppressive Microenvironment and Poor Prognosis
by Lin-Yu Chen, Rui-Lan Huang, Po-Hsuan Su, Ling-Hui Chu, Yu-Chun Weng, Hui-Chen Wang, Hung-Cheng Lai and Kuo-Chang Wen
Int. J. Mol. Sci. 2022, 23(9), 5120; https://doi.org/10.3390/ijms23095120 - 4 May 2022
Cited by 3 | Viewed by 2677
Abstract
Intraperitoneal metastasis is a challenging clinical scenario in epithelial ovarian cancer (EOC). As they are distinct from hematogenous metastasizing tumors, epithelial ovarian cancer cells primarily disseminate within the peritoneal cavity to form superficially invasive carcinomas. Unfavorable pharmacokinetics for peritoneal tumors and gut toxicity [...] Read more.
Intraperitoneal metastasis is a challenging clinical scenario in epithelial ovarian cancer (EOC). As they are distinct from hematogenous metastasizing tumors, epithelial ovarian cancer cells primarily disseminate within the peritoneal cavity to form superficially invasive carcinomas. Unfavorable pharmacokinetics for peritoneal tumors and gut toxicity collectively lead to a narrow therapeutic window and therefore limit the opportunities for a favorable clinical outcome. New insights into tumor metastasis in the peritoneal microenvironment are keenly awaited to develop new therapeutic strategies. Epithelial ovarian cancer stem cell (OCSC) seeding is considered to be a critical component of the peritoneal spread. Using a unique and stepwise process of the OCSC differentiation model may provide insight into the intraperitoneal metastasis. The transcriptome and epigenome of OCSC differentiation were characterized by expression array and MethylCap-Seq. The TCGA, AOCS, and KM-Plotter databases were used to evaluate the association between survival outcomes and the methylation/expression levels of candidate genes in the EOC datasets. The STRING database was used to investigate the protein–protein interaction (PPI) for candidates and their associated genes. The infiltration level of immune cells in EOC patients and the association between clinical outcome and OCSCs differentiation genes were estimated using the TIDE and TIME2.0 algorithms. We established an EOC differentiation model using OCSCs. After an integrated transcriptomics and methylomics analysis of OCSCs differentiation, we revealed that the genes associated with earlier OCSC differentiation were better able to reflect the patient’s outcome. The OCSC differentiation genes were involved in regulating metabolism shift and the suppressive immune microenvironment. High GPD1 expression with high pro-tumorigenic immune cells (M2 macrophage, and cancer associated fibroblast) had worst survival. Moreover, we developed a methylation signature, constituted by GNPDA1, GPD1, GRASP, HOXC11, and MSLN, that may be useful for prognostic prediction in EOC. Our results revealed a novel role of epigenetic plasticity OCSC differentiation and suggested metabolic and immune intervention as a new therapeutic strategy. Full article
(This article belongs to the Special Issue Ovarian Stem Cells)
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