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Life
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Recent Advances in Crop Genetics and Breeding
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Recent Advances in Crop Genetics and Breeding

A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Plant Science".

Deadline for manuscript submissions: 31 August 2025 | Viewed by 2152

Special Issue Editors

Dr. Tianxiao Chen

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Guest Editor
State Key Laboratory of Rice Biology, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China
Interests: genomics; crop biotic and abiotic tolerance; genetics and breeding; rice quality; salinity stress
Special Issues, Collections and Topics in MDPI journals
Dr. Xiangjin Wei

E-Mail Website
Guest Editor
China National Center for Rice Improvement and State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China
Interests: genetic improvement of rice quality; developmental biology of rice
Special Issues, Collections and Topics in MDPI journals
Dr. Baohua Feng

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Guest Editor
China National Rice Research Institute, Hangzhou, China
Interests: rice abiotic interaction; plant hormone; heat stress; low-temperature stress; salt stress; sugar metabolism
Special Issues, Collections and Topics in MDPI journals
Dr. Long Chen

E-Mail
Guest Editor
China National Rice Research Institute, Hangzhou, China
Interests: plant stress tolerance; rice genetics and breeding; biotechnology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues

Global climate change and unfavorable abiotic and biotic stresses are limiting agricultural productivity, intensifying the challenges encountered by crop scientists in meeting the rising global food supply demand. As such, there is an urgent need to produce better crop varieties by efficiently using genetic resources and molecular tools, wherein the elucidation of the genetic and molecular basis of useful crop traits are key to variety development. High-throughput genotyping systems assisted by bioinformatics and data science provide efficient and easy tools for geneticists and breeders. Precise target genome modification, the absence of foreign DNA in genome-edited plants, and the faster and cheaper method of genome modification are remarkable features of the current genome-editing technology that have resulted in its widespread application in crop breeding over less than a decade.

This Special Issue will focus on recent advances in crop genetics and breeding, inviting researchers to contribute original research papers and reviews on the potential themes including, but not limited to, the following:

  • Identification and functional analysis of QTL and genes for crop improvement;
  • Candidate gene and favorable allele identification for yield and biotic and abiotic stress tolerance;
  • Favorable haplotype-based molecular breeding;
  • Novel breeding techniques in crops, such as targeted mutagenesis, genome editing, etc.;
  • Genomic selection.

Dr. Tianxiao Chen
Dr. Xiangjin Wei
Dr. Baohua Feng
Dr. Long Chen
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Life is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • crop
  • high-throughput genotyping
  • genetic resources
  • favorable haplotype
  • marker-assisted selection
  • genome editing
  • CRISPR-Cas9
  • genomic selection

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Further information on MDPI's Special Issue polices can be found here.

Published Papers (2 papers)

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Research

13 pages, 3307 KiB  
Article
Generation and Assessment of Soybean (Glycine max (L.) Merr.) Hybrids for High-Efficiency Agrobacterium-Mediated Transformation
by Muhammad Waqar Khan, Aaqib Shaheen, Xuebin Zhang, Junli Zhang, Yaser Hassan Dewir and Katalin Magyar-Tábori
Life 2024, 14(12), 1649; https://doi.org/10.3390/life14121649 - 12 Dec 2024
Viewed by 732
Abstract
The Agrobacterium-mediated technique is widely employed for soybean transformation, but the efficiency of this method is still relatively modest, in which multiple factors are involved. Numerous chemical and physiological cues from host plants are needed for A. tumefaciens attraction and subsequent T-DNA [...] Read more.
The Agrobacterium-mediated technique is widely employed for soybean transformation, but the efficiency of this method is still relatively modest, in which multiple factors are involved. Numerous chemical and physiological cues from host plants are needed for A. tumefaciens attraction and subsequent T-DNA integration into the plant genome. Susceptible genotypes may permit this attachment and integration, and the agronomically superior genotypes with susceptibility to A. tumefaciens would play an important role in increasing transformation efficiency. In this study, we aimed to elevate the Agrobacterium-mediated transformation efficiency of soybean by integrating susceptibility alleles from William82 and flavonoids accumulating alleles from LX genotypes in the same soybean line. The crossing was made between LX () and William82 () soybean by hand pollination. Expectedly, the resulting hybrid soybean progenies inherited susceptibility traits and high flavonoid contents (i.e., genistein, genistin, apigenin, naringenin, quercetin, and cinnamic acid) essential for potential plant–pathogen interaction. Furthermore, the progenies and susceptible William82 soybean were subjected to transformation using A. tumefaciens (GV3101) harboring the GmUbi-3XFlag-35S-GFP and reassembled GmUbi3XFlag-35S-GFP: GUS vectors during separate events. Important transformation-related traits like shoot induction and shoot regeneration ability were also significantly improved in progenies. The progenies designated as ZX-3 exhibited superiority over the William82 parental line in all three traits, i.e., shoot induction, regeneration, and Agrobacterium-mediated transformation. The transient transformation efficiency of the ZX-16 line was remarkably higher when half-cotyledon explants were wounded and transformed with A. tumefaciens harboring GUS assembly vector and then co-cultivated on MS medium supplemented with 2 mg/L spermidine, 0.3 g/L GA3, 0.3 mg/L kinetin, and 1.3 mg/L 6-benzylaminopurine. In addition, the shoot elongation was also higher than that of William82 after two weeks of culture on the shoot induction medium. The newly generated soybeans have the potential to serve as a valuable source for high transgene production and represent a promising avenue for future soybean varietal development. Full article
(This article belongs to the Special Issue Recent Advances in Crop Genetics and Breeding)
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17 pages, 4903 KiB  
Article
Functional Characterisation of GmGASA1-like Gene in Glycine max (L.) Merr.: Overexpression Promotes Growth, Development and Stress Responses
by Mohamed A. S. Khalifa, Qi Zhang, Yeyao Du, Nooral Amin, Baozhu Dong and Piwu Wang
Life 2024, 14(11), 1436; https://doi.org/10.3390/life14111436 - 6 Nov 2024
Viewed by 835
Abstract
The presence of Gibberellic Acid-Stimulated Arabidopsis, GASA, gene family has been reported in many important plants, playing roles in various aspects of plant biology but little has been uncovered in soybeans. Soybean is one of the major plants providing [...] Read more.
The presence of Gibberellic Acid-Stimulated Arabidopsis, GASA, gene family has been reported in many important plants, playing roles in various aspects of plant biology but little has been uncovered in soybeans. Soybean is one of the major plants providing nutrition for humans and livestock globally. In this study, we overexpressed a novel GASA gene (GmGASA1-like) in Glycine max and conducted bioinformatic analyses, evaluated the T2 transgenic line in an open field, and applied major stressors along with the growth promoter GA3 to investigate the potential functions of the GmGASA1-like gene. The results of bioinformatics implied that the GmGASA1-like gene is regulated by GA3, and its protein has the potential to influence key processes of plant growth and development. The transgenic plants (JN74-OE) were significantly taller and had a larger canopy in the field trial at the R1-growth stage and demonstrated superiority in some seed quantity and quality traits after harvesting. Under abiotic stresses (including cold, heat, and drought) and spraying of GA3, the level of GmGASA1-like gene expression in JN74-OE exceeded the levels measured before the treatments. Notably, the highest expression level was observed during the drought stress treatment. Photosynthesis pigments levels in both the overexpressed lines and the control group showed no significant differences. In summary, this study sheds light on the multifaceted roles of the GmGASA1-like gene, impacting soybean plant architecture, seed traits, and stress responses. Ultimately, this research paves the way for a more productive and sustainable soybean industry. Full article
(This article belongs to the Special Issue Recent Advances in Crop Genetics and Breeding)
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