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Advances in Platelet-rich Fibrin for Bone Tissue Engineering

A special issue of Materials (ISSN 1996-1944). This special issue belongs to the section "Biomaterials".

Deadline for manuscript submissions: closed (31 August 2020) | Viewed by 7528

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Clinic for Maxillofacial and Plastic Surgery, Johann Wolfgang Goethe University, FORM-Lab (Frankfurt Oral Regenerative Medicine), 60596 Frankfurt Am Main, Germany
Interests: cell culture; molecular biology; cell biology; biomaterials; tissue engineering; biocompatibility; molecular pathology; scaffolds
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Dear Colleagues,

The use of bioactive biomaterials in tissue engineering, especially platelet-rich fibrin (PRF), has gained increasing importance in the last few years. This special group of autologous and bioactive biomaterials provides signaling molecules, growth factors, and cytokines that support the regeneration process. Great efforts have been undertaken to understand the composition and bioactivity of differently prepared platelet-rich fibrin matrices and their interactions with cells. Recent studies have outlined the significance of the preparation protocol and the applied centrifugal force for the cellular content and structural characters of the resulting fibrin-based biomaterials. However, still little is known about the benefits and mechanisms of action of PRF in bone regeneration. Therefore, further research is essential to outline these aspects and provide additional scientific evidence in this field.

This Special Issue will provide an overview of different aspects related to Advances in platelet-rich fibrin for Bone Tissue Engineering, from the bench to the clinic. It is my pleasure to invite you to submit your work as systematic reviews, short communications, original studies addressing preclinical in vitro and in vivo work, and controlled clinical studies reporting preliminary clinical outcomes.

Prof. Dr. Shahram M. Ghanaati
Guest Editor

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Keywords

  • Platelet rich fibrin
  • PRF
  • Tissue engineering
  • Bone regeneration
  • Autologous bioactive materials

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Published Papers (2 papers)

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Research

13 pages, 4623 KiB  
Article
Effects of Platelet-Rich Fibrin on Hard Tissue Healing: A Histomorphometric Crossover Trial in Sheep
by Esra Ondur, Nilufer Bolukbasi Balcioglu, Merva Soluk Tekkesin, Ozlem Guzel and Selim Ersanli
Materials 2020, 13(7), 1695; https://doi.org/10.3390/ma13071695 - 4 Apr 2020
Cited by 4 | Viewed by 2478
Abstract
Bone defects lead to aesthetic and functional losses, causing dental rehabilitation to be more difficult. The objective of this work is to histologically assess the hard tissue healing of bone defects filled with platelet-rich fibrin (PRF) alone or as an adjuvant for mixing [...] Read more.
Bone defects lead to aesthetic and functional losses, causing dental rehabilitation to be more difficult. The objective of this work is to histologically assess the hard tissue healing of bone defects filled with platelet-rich fibrin (PRF) alone or as an adjuvant for mixing with and covering anorganic bovine bone (ABB), compared to ABB covered with a resorbable collagen membrane (CM). This study was designed as a crossover animal study. Four 5-mm tibia defects, 5 mm apart from each other, were surgically created on the tibias of 6 sheep. The defects were randomly filled with ABB + CM; PRF alone; ABB+PRF; or were left empty. The animals were euthanized on days 10, 20, and 40 post-operatively. No group showed any signs of bone necrosis. Inflammation was observed in 2 control and 3 test defects with no statistically significant difference between groups at each time point. The ABB + CM and ABB + PRF groups experienced the highest bone regeneration ratios. No differences between the empty-defect and PRF groups were observed in regard to bone regeneration. No statistical difference was observed between the ABB+PRF and ABB + CM groups in regard to bone regeneration and the amount of residual graft material at each time point. The use of PRF should be preferred due to its autogenous origin, low cost, and ease of use. Full article
(This article belongs to the Special Issue Advances in Platelet-rich Fibrin for Bone Tissue Engineering)
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17 pages, 4088 KiB  
Article
Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering
by Sarah Al-Maawi, Carlos Herrera-Vizcaíno, Anna Orlowska, Ines Willershausen, Robert Sader, Richard J Miron, Joseph Choukroun and Shahram Ghanaati
Materials 2019, 12(23), 3993; https://doi.org/10.3390/ma12233993 - 2 Dec 2019
Cited by 34 | Viewed by 4533
Abstract
Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on the previously described [...] Read more.
Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on the previously described low-speed centrifuge concept (LSCC), which allowed the introduction of a liquid-PRF formulation of fibrinogen and thrombin prior to its conversion to fibrin. Liquid-PRF was introduced to meet the clinical demand for combination with biomaterials in a clinically applicable and easy-to-use way. The aim of the present study was to evaluate, ex vivo, the interaction of the liquid-PRF constituents with five different collagen biomaterials by histological analyses. The results first demonstrated that large variability existed between the biomaterials investigated. Liquid-PRF was able to completely invade Mucograft® (MG; Geistlich Biomaterials, Wolhusen, Switzerland) and to partly invade Bio-Gide® (BG; Geistlich Biomaterials, Wolhusen, Switzerland) and Mucoderm® (MD; Botiss Biomaterials, Berlin, Germany), and Collprotect® (CP; Botiss Biomaterials, Berlin, Germany) showed only a superficial interaction. The BEGO® collagen membrane (BCM; BEGO Implant Systems) appeared to be completely free of liquid-PRF. These results were confirmed by the different cellular penetration and liquid-PRF absorption coefficient (PAC) values of the evaluated membranes. The present study demonstrates a system for loading biomaterials with a complex autologous cell system (liquid-PRF) in a relatively short period of time and in a clinically relevant manner. The combination of biomaterials with liquid-PRF may be clinically utilized to enhance the bioactivity of collagen-based biomaterials and may act as a biomaterial-based growth factor delivery system. Full article
(This article belongs to the Special Issue Advances in Platelet-rich Fibrin for Bone Tissue Engineering)
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