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Nanomaterials
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Nanoscale and Sub-Nanoscale Applications of New Fluorescent Materials
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Nanoscale and Sub-Nanoscale Applications of New Fluorescent Materials

A special issue of Nanomaterials (ISSN 2079-4991). This special issue belongs to the section "Nanophotonics Materials and Devices".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 9477

Special Issue Editor

Dr. Olaf Rolinski

E-Mail Website
Guest Editor
Dept. of Physics, Univ. of Strathclyde, Glasgow, Scotland, UK
Interests: biomolecules; nanoparticles; quantum dots; protein aggregation/oligomerisation; fluorescence kinetics; fluorescence lifetimes; FRET

Special Issue Information

Dear Colleagues,

Fluorescent materials (natural fluorophores, fluorescent sensors, metal nanoparticles, quantum dots, etc.) have found numerous applications in diverse areas of science where sub-nm spatial resolution is essential. More recently, man-made materials, such as metal nanoparticles and quantum dots, have demonstrated high applicability due to their advantages over aromatic fluorophores: chemical inertness, biocompatibility, high water solubility, resistance to photobleaching, opportunity for surface functionalisation, low toxicity and tuneable optical properties. These features make them ideal fluorophores for fluorescence sensing, bioimaging, photocatalysis, drug delivery, or theranostic applications, underpinning key aspects of bio and medical sciences.

This Special Issue of Nanomaterials aims to cover the recent advancements in new fluorescent materials and new fluorescence spectroscopy methods for researching processes occurring in the nm scale and the sub-nm scale, which is currently beyond all current microscopies.

Dr. Olaf Rolinski
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Nanomaterials is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescent sensors
  • biomolecules
  • nanoparticles
  • quantum dots
  • fluorescence time-resolved kinetics
  • FRET
  • structural changes in nanometre scale
  • protein conformations
  • particle aggregation/oligomerisation

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Published Papers (3 papers)

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Research

14 pages, 4632 KiB  
Article
Long-Term Fluorescence Behavior of CdSe/ZnS Quantum Dots on Various Planar Chromatographic Stationary Phases
by Paweł K. Zarzycki
Nanomaterials 2022, 12(5), 745; https://doi.org/10.3390/nano12050745 - 23 Feb 2022
Viewed by 1801
Abstract
Nanoparticles, particularly quantum dots (QDs), are commonly used for the sensitive detection of various objects. A number of target molecules may be determined using QDs sensing systems. Depending on their chemical nature, physicochemical properties, and spatial arrangement, QDs can selectively interact with given [...] Read more.
Nanoparticles, particularly quantum dots (QDs), are commonly used for the sensitive detection of various objects. A number of target molecules may be determined using QDs sensing systems. Depending on their chemical nature, physicochemical properties, and spatial arrangement, QDs can selectively interact with given molecules of interest. This can be performed in complex systems, including microorganisms or tissues. Efficient fluorescence enables low exposure of QDs and high sensitivity for detection. One disadvantage of quantum dots fluorophores is fluorescence decay. However, for given applications, this property may be an advantage, e.g., for highly sensitive detection based on correlation images in the time domain. This experimental work deals with the measurement of fluorescence decay of Lumidot TMCdSe/ZnS (530 nm) quantum dots. These nanoparticles were transferred to the surface of various planar chromatographic stationary phases. Fluorescence of formed spots was recorded at room temperature over a long period of time, namely 15.7824 × 105 min (three years). The resulting signal profiles in the time domain were analyzed using classical approach (luminescence model comparison involving different mathematical models).Moreover, fluorescence behavior on different TLC/HPTLC supports was investigated using multivariate statistics (principal component analysis, PCA). Eight planar chromatographic stationary phases were investigated, including cellulose, octadecylsilane, polyamide, silica gel and aluminium oxide in different forms (TLC and HPTLC types). The presented research revealed significantly different and non-linear long-term QDs behavior on these solids. Two different fluorescence signal trajectories were recorded, including typical signal decay after QDs application to the plates and long-term intensity increase. This was particularly visible for given planar chromatographic adsorbents, e.g., cellulose or octadecylsilane. To the author’s knowledge, these findings were not reported before using the stationary chromatographic phases, and enable the design of future experiments toward sensing of low molecular mass chemicals using, e.g., advanced quantification approaches. This may include signal processing computations based on correlation images in the time domain. Additionally, the reported preliminary data indicates that the investigated nanoparticles can be applied as efficient and selective fluorophores. This was demonstrated on micro-TLC plates where separated bioactive organic substances quenching from cyanobacteria extracts were sensitively detected. The described detection protocol can be directly applied for different planar chromatographic systems, including paper-based microfluidic devices, planar electrophoresis and/or miniaturized microfluidic chip devices. Full article
(This article belongs to the Special Issue Nanoscale and Sub-Nanoscale Applications of New Fluorescent Materials)
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11 pages, 4883 KiB  
Article
Engineering Red-Enhanced and Biocompatible Upconversion Nanoparticles
by Masfer Alkahtani, Najla Alsofyani, Anfal Alfahd, Anas A. Almuqhim, Fahad A. Almughem, Abdullah A. Alshehri, Hussam Qasem and Philip R. Hemmer
Nanomaterials 2021, 11(2), 284; https://doi.org/10.3390/nano11020284 - 22 Jan 2021
Cited by 20 | Viewed by 3096
Abstract
The exceptional optical properties of lanthanide-doped upconversion nanoparticles (UCNPs) make them among the best fluorescent markers for many promising bioapplications. To fully utilize the unique advantages of the UCNPs for bioapplications, recent significant efforts have been put into improving the brightness of small [...] Read more.
The exceptional optical properties of lanthanide-doped upconversion nanoparticles (UCNPs) make them among the best fluorescent markers for many promising bioapplications. To fully utilize the unique advantages of the UCNPs for bioapplications, recent significant efforts have been put into improving the brightness of small UCNPs crystals by optimizing dopant concentrations and utilizing the addition of inert shells to avoid surface quenching effects. In this work, we engineered bright and small size upconversion nanoparticles in a core–shell–shell (CSS) structure. The emission of the synthesized CSS UCNPs is enhanced in the biological transparency window under biocompatible excitation wavelength by optimizing dopant ion concentrations. We also investigated the biosafety of the synthesized CSS UCNP particles in living cell models to ensure bright and non-toxic fluorescent probes for promising bioapplications. Full article
(This article belongs to the Special Issue Nanoscale and Sub-Nanoscale Applications of New Fluorescent Materials)
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8 pages, 1979 KiB  
Communication
Optimization of Wide-Field ODMR Measurements Using Fluorescent Nanodiamonds to Improve Temperature Determination Accuracy
by Tamami Yanagi, Kiichi Kaminaga, Wataru Kada, Osamu Hanaizumi and Ryuji Igarashi
Nanomaterials 2020, 10(11), 2282; https://doi.org/10.3390/nano10112282 - 18 Nov 2020
Cited by 7 | Viewed by 4138
Abstract
Fluorescent nanodiamonds containing nitrogen-vacancy centers have attracted attention as nanoprobes for temperature measurements in microenvironments, potentially enabling the measurement of intracellular temperature distributions and temporal changes. However, to date, the time resolution and accuracy of the temperature determinations using fluorescent nanodiamonds have been [...] Read more.
Fluorescent nanodiamonds containing nitrogen-vacancy centers have attracted attention as nanoprobes for temperature measurements in microenvironments, potentially enabling the measurement of intracellular temperature distributions and temporal changes. However, to date, the time resolution and accuracy of the temperature determinations using fluorescent nanodiamonds have been insufficient for wide-field fluorescence imaging. Here, we describe a method for highly accurate wide-field temperature imaging using fluorescent nanodiamonds for optically detected magnetic resonance (ODMR) measurements. We performed a Monte Carlo simulation to determine the optimal frequency sweep range for ODMR temperature determination. We then applied this sweep range to fluorescent nanodiamonds. As a result, the temperature determination accuracies were improved by a factor ~1.5. Our result paves the way for the contribution of quantum sensors to cell biology for understanding, for example, differentiation in multicellular systems. Full article
(This article belongs to the Special Issue Nanoscale and Sub-Nanoscale Applications of New Fluorescent Materials)
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