Lower body positive pressure treadmills (LBPPTs) as a strategy to reduce musculoskeletal load are becoming more common as part of sports conditioning, although the requisite physiological parameters are unclear. To elucidate their role, ten well-trained runners (30.2 ± 3.4 years; VO
2max: 60.3 ± 4.2 mL kg
−1 min
−1) ran at 70% of their individual velocity at VO
2max (vVO
2max) on a LBPPT at 80% body weight support (80% BW
Set) and 90% body weight support (90% BW
Set), at 0%, 2% and 7% incline. Oxygen consumption (VO
2), heart rate (HR) and blood lactate accumulation (LA) were monitored. It was found that an increase in incline led to increased VO
2 values of 6.8 ± 0.8 mL kg
−1 min
−1 (0% vs. 7%,
p < 0.001) and 5.4 ± 0.8 mL kg
−1 min
−1 (2% vs. 7%,
p < 0.001). Between 80% BW
Set and 90% BW
Set, there were VO
2 differences of 3.3 ± 0.2 mL kg
−1 min
−1 (
p < 0.001). HR increased with incline by 12 ± 2 bpm (0% vs. 7%,
p < 0.05) and 10 ± 2 bpm (2% vs. 7%,
p < 0.05). From 80% BW
Set to 90% BW
Set, HR increases of 6 ± 1 bpm (
p < 0.001) were observed. Additionally, LA values showed differences of 0.10 ± 0.02 mmol l
−1 between 80% BW
Set and 90% BW
Set. Those results suggest that on a LBPPT, a 2% incline (at 70% vVO
2max) is not yet sufficient to produce significant physiological changes in VO
2, HR and LA—as opposed to running on conventional treadmills, where significant changes are measured. However, a 7% incline increases VO
2 and HR significantly. Bringing together physiological and biomechanical factors from previous studies into this practical context, it appears that a 7% incline (at 80% BW
Set) may be used to keep VO
2 and HR load unchanged as compared to unsupported running, while biomechanical stress is substantially reduced.
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