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Plants
Volume 11
Issue 23
10.3390/plants11233319
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Brief Report
Peer-Review Record

Elicitation of Hyoscyamine Production in Datura stramonium L. Plants Using Tobamoviruses

Plants 2022, 11(23), 3319; https://doi.org/10.3390/plants11233319
by Daniel Mihálik 1,2, Richard Hančinský 1, Šarlota Kaňuková 2, Michaela Mrkvová 1,2 and Ján Kraic 1,2,*
Reviewer 1:
Ahmed Abdelkhalek
Reviewer 2:
Mario Simirgiotis
Reviewer 3: Anonymous
Plants 2022, 11(23), 3319; https://doi.org/10.3390/plants11233319
Submission received: 6 November 2022 / Revised: 25 November 2022 / Accepted: 29 November 2022 / Published: 1 December 2022
(This article belongs to the Special Issue Medicinal Plant Extracts)

Round 1

Reviewer 1 Report

I have one concerning comment regarding how we can use plant viruses as an elicitor. I think this is can not be applicable, especially under open field conditions.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

In this manuscript Live viruses are used as elicitors in  D. stramonium plants grown in soil which were  artificially infected with the tobamoviruses Pepper mild mottle virus (PMMoV), Tomato mosaic virus (ToMV), and Tobacco mosaic virus (TMV). Differences in the content of hyoscyamine were between capsules and roots of infected and non-infected plants. Elicitation increased content of
hyoscyamine in capsules 1.23–2.34 times, compared to the control. The most effective viruses were reported PMMoV and ToMV (isolate PV143), which increased content to above 19 mg/g of fresh weight of ther capsule. good charts and results are consistent with the research done

Comments check English language

It would be good to add recovery of hiosciamine in the biological material as well as LOD LOQ and other common paprameters for validation of measurements by GC  and internal standard

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 3 Report

I have read with much interest the MS submitted. The design is appropriate and interpretation is supported with data. The research gap has been properly identified.  I have some concerns that needs to be addressed: 

In abstract: What is the difference between: ''Tomato mosaic virus (ToMV), and Tobacco mosaic virus (TMV).'  !

I would suggest more data/figures in the abstract.

e.g. Differences in the content of hyoscyamine were between capsules and roots of infected and non-infected plants should be support with stats + data

Please refer to lines 19-20 in abstract ‘’ increased content of hyoscyamine in capsules 1.23–2.34 times. Was it significant?

In section 4.1. Please elaborate how many plants were included in the sample.

Elaborate how authors identified plants were ‘’healthy’’- L179  

Author Response

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Author Response File: Author Response.docx

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