Advances and Limitations of In Vitro Embryo Production Technologies in Small Ruminants

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Small Ruminants".

Deadline for manuscript submissions: 28 February 2025 | Viewed by 1008

Special Issue Editors


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Guest Editor
Department of Animal Reproduction and Developmental Sciences, China Agricultural University, Beijing, China
Interests: domestic animals; sperm and oocyte recognition; fertilization; embryo

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Guest Editor
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China
Interests: embryo implantation, in vitro culture; spermatogenesis; conceptus elongation; embryo models

Special Issue Information

Dear Colleagues,

The procedures for in vitro embryo production (IVEP) have been widely applied in domestic animal breeding to accelerate the genetic spread of females. The combination of IVEP and embryo cryoconservation has contributed to shortening generation intervals and increased genetic gain for dairy cows. Compared to embryos produced in vivo, the efficiency of IVEP for small ruminants is still not ideal and limits its commercial application. The IVEP methodology involves the following steps: the oocytes recovered from follicles are maturated (IVM), fertilized in vitro (IVF), and cultured in vitro (IVC) up to the blastocyst stage. Recently, research using advanced molecular methods, particularly multi-omics sequencing at the single cell level, has clarified many issues related to oocyte maturation and embryo development, which will soon be applied to improve the use of IVEP for small ruminants.

In this Special Issue, we invite original research papers and reviews or studies addressing the advances and limitations of in vitro embryo production technologies in small ruminants, including inducing ovulation, oocyte aspiration, oocyte maturation, fertilization, and embryo culture in vitro.

Dr. Lu Zhang
Dr. Gongxue Jia
Guest Editors

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Keywords

  • small ruminants
  • goat
  • sheep
  • IVEP
  • in vitro
  • fertilization
  • embryo
  • assisted reproductive technology

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Published Papers (1 paper)

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Research

13 pages, 1131 KiB  
Article
The Influence of Oviductal and Uterine Fluid Supplementation on the In Vitro Development and Quality of Cloned Sheep Embryos
by José Roberto Vazquez-Avendaño, César Cortez-Romero, Demetrio Alonso Ambríz-García, José Luis Rodríguez-Suástegui, José Ernesto Hernández-Pichardo and María del Carmen Navarro-Maldonado
Animals 2024, 14(19), 2894; https://doi.org/10.3390/ani14192894 - 8 Oct 2024
Viewed by 708
Abstract
Somatic cell nuclear transfer (SCNT) has great potential for the replication of high-commercial-value animals, threatened wild species for conservation purposes, and transgenic animals for biomedical purposes. However, SCNT has a low success rate due to intrinsic factors of the technique itself, which leads [...] Read more.
Somatic cell nuclear transfer (SCNT) has great potential for the replication of high-commercial-value animals, threatened wild species for conservation purposes, and transgenic animals for biomedical purposes. However, SCNT has a low success rate due to intrinsic factors of the technique itself, which leads to low rates of embryonic development and epigenetic alterations in cloned embryos. The objective of this study was to evaluate the effect of OF–UF on the intracellular concentrations of ROS and GSH and the development of cloned and parthenogenetic Ovis aries embryos. The results do not show a beneficial effect on the development of parthenogenetic and cloned embryos at concentrations of 0.5% OF–UF. Furthermore, at 1% OF–UF, an adverse effect was observed in cloned embryos at the blastocyst stage and 2% OF and UF in parthenogenetic embryos during the first divisions. Decreases in ROS and GSH levels were observed in the parthenogenetic blastocysts treated with 1% OF–UF, but not in the clones, in which a higher concentration of GSH and a similar concentration of ROS were observed. No effect of OF–UF was observed on embryonic development and redox balance in sheep embryos cloned via handmade cloning. Full article
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