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Biosensors
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Fluorescent Sensors for Biological Applications
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Fluorescent Sensors for Biological Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: closed (14 April 2024) | Viewed by 5324

Special Issue Editors

Dr. Luling Wu

E-Mail Website
Guest Editor
Department of Chemistry, University of Bath, Bath BA2 7AY, UK
Interests: fluorescent sensor design; molecular recognition; theranostic systems; probes for redox imbalance
Prof. Dr. Guang-Bo Ge

E-Mail Website
Guest Editor
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Interests: enzymatic biosensors; optical substrates; hydrolases; cytochrome P450 enzymes; transferases
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The past few decades have witnessed extraordinary advances in fluorescent sensors that have revolutionized the way biology can be studied. Fluorescent sensors are usually based on fluorescent organic molecules, nanoparticles, proteins, or combinations of organic molecules, nanoparticles, and proteins. They are designed and engineered to change their fluorescent colors or intensities in response to external stimuli or physiological changes, including pH fluctuations, metal ion homeostasis, cell signaling, membrane potential differences, phosphorylation, ubiquitination, redox reactions, and apoptosis.

In particular, the 2008 Nobel Prize in Chemistry was awarded to three scientists who discovered and developed fluorescent proteins, which have now been developed into a very large group of biosensors.

This Special Issue will focus on the use of fluorescent sensors in biological applications through an interdisciplinary approach. Our goal is to bring together researchers active in the development of innovative optically activated materials for the advancement of biophotonics. Both review articles and original research papers are welcome. There is a particular interest in papers concerning new biomedicine frontiers that may be surpassed through fluorescence imaging or sensing.

We look forward to your submissions.

Dr. Luling Wu
Prof. Dr. Guang-Bo Ge
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescent probes
  • fluorescence imaging
  • fluorescent labeling
  • multiple-responsive
  • fluorescent prodrugs

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Published Papers (3 papers)

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14 pages, 5565 KiB  
Article
A Novel Fluorescence-Based Microplate Assay for High-Throughput Screening of hSULT1As Inhibitors
by Xiaoting Niu, Yufan Fan, Liwei Zou and Guangbo Ge
Biosensors 2024, 14(6), 275; https://doi.org/10.3390/bios14060275 - 27 May 2024
Viewed by 1110
Abstract
Human sulfotransferase 1As (hSULT1As) play a crucial role in the metabolic clearance and detoxification of a diverse range of endogenous and exogenous substances, as well as in the bioactivation of some procarcinogens and promutagens. Pharmacological inhibiting hSULT1As activities may enhance the in vivo [...] Read more.
Human sulfotransferase 1As (hSULT1As) play a crucial role in the metabolic clearance and detoxification of a diverse range of endogenous and exogenous substances, as well as in the bioactivation of some procarcinogens and promutagens. Pharmacological inhibiting hSULT1As activities may enhance the in vivo effects of most hSULT1As drug substrates and offer protective strategies against the hSULT1As-mediated bioactivation of procarcinogens. To date, a fluorescence-based high-throughput assay for the efficient screening of hSULT1As inhibitors has not yet been reported. In this work, a fluorogenic substrate (HN-241) for hSULT1As was developed through scaffold-seeking and structure-guided molecular optimization. Under physiological conditions, HN-241 could be readily sulfated by hSULT1As to form HN-241 sulfate, which emitted brightly fluorescent signals around 450 nm. HN-241 was then used for establishing a novel fluorescence-based microplate assay, which strongly facilitated the high-throughput screening of hSULT1As inhibitors. Following the screening of an in-house natural product library, several polyphenolic compounds were identified with anti-hSULT1As activity, while pectolinarigenin and hinokiflavone were identified as potent inhibitors against three hSULT1A isozymes. Collectively, a novel fluorescence-based microplate assay was developed for the high-throughput screening and characterization of hSULT1As inhibitors, which offered an efficient and facile approach for identifying potent hSULT1As inhibitors from compound libraries. Full article
(This article belongs to the Special Issue Fluorescent Sensors for Biological Applications)
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16 pages, 14064 KiB  
Article
A Copper-Selective Sensor and Its Inhibition of Copper-Amyloid Beta Aggregation
by Ngoc Kim Nguyen, Bella Poduska, Mia Franks, Manoranjan Bera, Ian MacCormack, Guoxing Lin, Alexander P. Petroff, Samir Das and Arundhati Nag
Biosensors 2024, 14(5), 247; https://doi.org/10.3390/bios14050247 - 14 May 2024
Cited by 1 | Viewed by 1831
Abstract
Copper is an essential trace metal for biological processes in humans and animals. A low level of copper detection at physiological pH using fluorescent probes is very important for in vitro applications, such as the detection of copper in water or urine, and [...] Read more.
Copper is an essential trace metal for biological processes in humans and animals. A low level of copper detection at physiological pH using fluorescent probes is very important for in vitro applications, such as the detection of copper in water or urine, and in vivo applications, such as tracking the dynamic copper concentrations inside cells. Copper homeostasis is disrupted in neurological diseases like Alzheimer’s disease, and copper forms aggregates with amyloid beta (Ab42) peptide, resulting in senile plaques in Alzheimer’s brains. Therefore, a selective copper detector probe that can detect amyloid beta peptide-copper aggregates and decrease the aggregate size has potential uses in medicine. We have developed a series of Cu2+-selective low fluorescent to high fluorescent tri and tetradentate dentate ligands and conjugated them with a peptide ligand to amyloid-beta binding peptide to increase the solubility of the compounds and make the resultant compounds bind to Cu2+–amyloid aggregates. The copper selective compounds were developed using chemical scaffolds known to have high affinity and selectivity for Cu2+, and their conjugates with peptides were tested for affinity and selectivity towards Cu2+. The test results were used to inform further improvement of the next compound. The final Cu2+ chelator–peptide conjugate we developed showed high selectivity for Cu2+ and high fluorescence properties. The compound bound 1:1 to Cu2+ ion, as determined from its Job’s plot. Fluorescence of the ligand could be detected at nanomolar concentrations. The effect of this ligand on controlling Cu2+–Ab42 aggregation was studied using fluorescence assays and microscopy. It was found that the Cu2+–chelator–peptide conjugate efficiently reduced aggregate size and, therefore, acted as an inhibitor of Ab42-Cu2+ aggregation. Since high micromolar concentrations of Cu2+ are present in senile plaques, and Cu2+ accelerates the formation of toxic soluble aggregates of Ab42, which are precursors of insoluble plaques, the developed hybrid molecule can potentially serve as a therapeutic for Alzheimer’s disease. Full article
(This article belongs to the Special Issue Fluorescent Sensors for Biological Applications)
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13 pages, 2871 KiB  
Article
A Ratiometric Biosensor Containing Manganese Dioxide Nanosheets and Nitrogen-Doped Quantum Dots for 2,4-Dichlorophenoxyacetic Acid Monitoring
by Yang Guo, Jingran Sun, Mingzhu Liu, Jin Wu, Zunquan Zhao, Ting Ma and Yanjun Fang
Biosensors 2024, 14(2), 63; https://doi.org/10.3390/bios14020063 - 24 Jan 2024
Viewed by 1846
Abstract
Nanomaterials are desirable for sensing applications. Therefore, MnO2 nanosheets and nitrogen-doped carbon dots (NCDs) were used to construct a ratiometric biosensor for quantification of 2,4-dichlorophenoxyacetic acid. The MnO2 nanosheets drove the oxidation of colorless o-phenylenediamine to OPDox, which exhibits fluorescence emission [...] Read more.
Nanomaterials are desirable for sensing applications. Therefore, MnO2 nanosheets and nitrogen-doped carbon dots (NCDs) were used to construct a ratiometric biosensor for quantification of 2,4-dichlorophenoxyacetic acid. The MnO2 nanosheets drove the oxidation of colorless o-phenylenediamine to OPDox, which exhibits fluorescence emission peaks at 556 nm. The fluorescence of OPDox was efficiently quenched and the NCDs were recovered as the ascorbic acid produced by the hydrolyzed alkaline phosphatase (ALP) substrate increased. Owing to the selective inhibition of ALP activity by 2,4-D and the inner filter effect, the fluorescence intensity of the NCDs at 430 nm was suppressed, whereas that at 556 nm was maintained. The fluorescence intensity ratio was used for quantitative detection. The linear equation was F = 0.138 + 3.863·C 2,4-D (correlation coefficient R2 = 0.9904), whereas the limits of detection (LOD) and quantification (LOQ) were 0.013 and 0.040 μg/mL. The method was successfully employed for the determination of 2,4-D in different vegetables with recoveries of 79%~105%. The fluorescent color change in the 2,4-D sensing system can also be captured by a smartphone to achieve colorimetric detection by homemade portable test kit. Full article
(This article belongs to the Special Issue Fluorescent Sensors for Biological Applications)
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