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Biosensors
Special Issues
Microfluidics System and Its Application in Single Cell Analysis
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Microfluidics System and Its Application in Single Cell Analysis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (30 May 2022) | Viewed by 17751

Special Issue Editors

Prof. Dr. Ying Mu

E-Mail Website
Guest Editor
College of Control Science and Engineering, Zhejiang University, Hangzhou 310007, China
Interests: microfluidics system and its application in life sciences; surface plasmon resonance biosensors; integrated nanobiotechnology
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Qiangyuan Zhu

E-Mail Website
Guest Editor
College of Control Science and Engineering, Zhejiang University, Hangzhou 310007, China
Interests: high throughput single cell genomic sequencing; biomicrofluidic chip; digital PCR; deep learning and artificial intelligence
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Single-cell analysis techniques based on the microfluidics system have become a powerful tool, as well as a driving force for biological studies and discoveries. This growing field has increased the sensitivity, accuracy, and throughput of traditional single-cell analysis methods. Single-cell sequencing methods based on the microfluidics system have enabled rapid genome-wide analysis of thousands of single cells within one experiment. These cutting-edge methods have allowed us to profile cells at unprecedented resolutions and numbers, thus building a complete human cell atlas. Such a comprehensive database would inform us of the fundamental features of each cell type and provide insights into the changes and underlying mechanisms behind embryonic development, as well as disease onset and progression. The microfluidics system also contributes to human tumor atlases, deciphering this complex issue involving a high degree of heterogeneity among different cell populations and their interactions.

For this Special Issue, titled "The Microfluidics System and Its Application in Single-Cell Analysis", we welcome original works, perspectives, and reviews focusing on developments and applications of single-cell analysis methods based on the microfluidics system, including single-cell capturing and culture, imaging, protein analysis, nucleic acid analysis, and genomics, transcriptomics, spatial transcriptomics, epigenomics, and multi-omics analyses.

Prof. Dr. Ying Mu
Prof. Dr. Qiangyuan Zhu
Guest Editors

If you want to learn more information or need any advice, you can contact the Special Issue Editor Jessica Zhou via <[email protected]> directly.

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

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Published Papers (3 papers)

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Research

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12 pages, 3051 KiB  
Article
A Specific and Sensitive Aptamer-Based Digital PCR Chip for Salmonella typhimurium Detection
by Yuanjie Suo, Weihong Yin, Qiangyuan Zhu, Wenshuai Wu, Wenjian Cao and Ying Mu
Biosensors 2022, 12(7), 458; https://doi.org/10.3390/bios12070458 - 26 Jun 2022
Cited by 13 | Viewed by 3076
Abstract
Food poisoning and infectious diseases caused by Salmonella typhimurium (S. typhimurium) are serious public health concerns for human health and food safety. The diversity and complexity of food matrices pose great challenges for rapid and ultra-sensitive detection of S. typhimurium in [...] Read more.
Food poisoning and infectious diseases caused by Salmonella typhimurium (S. typhimurium) are serious public health concerns for human health and food safety. The diversity and complexity of food matrices pose great challenges for rapid and ultra-sensitive detection of S. typhimurium in food samples. A method capable of identification, detection, and quantification of S. typhimurium is essential for addressing these issues. In this study, aptamer-coated magnetic beads (Apt-MBs) are employed as capture bio-probes to specifically and selectively concentrate S. typhimurium in food samples. A self-priming chip-based digital PCR was then presented as another biosensor for on-site detection and quantification of S. typhimurium cells. The chip we developed was robust and did not require any external power for sample loading. The combination of Apt-MBs with an on-chip digital detection realized the integration into lab-on-a-chip-based biosensors for on-site monitoring of foodborne pathogens. It was possible to capture and detect S. typhimurium cells as low as 90 CFU/reaction with a capture efficiency of 94.5%. Additionally, the whole process only took about 2 h. This unique platform could also be used to monitor other target bacteria with high specificity and sensitivity by utilizing different aptamers. Furthermore, the platform has potential applications in point-of-care testing in the future. Full article
(This article belongs to the Special Issue Microfluidics System and Its Application in Single Cell Analysis)
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Review

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22 pages, 3735 KiB  
Review
Microfluidics Facilitates the Development of Single-Cell RNA Sequencing
by Yating Pan, Wenjian Cao, Ying Mu and Qiangyuan Zhu
Biosensors 2022, 12(7), 450; https://doi.org/10.3390/bios12070450 - 24 Jun 2022
Cited by 9 | Viewed by 7865
Abstract
Single-cell RNA sequencing (scRNA-seq) technology provides a powerful tool for understanding complex biosystems at the single-cell and single-molecule level. The past decade has been a golden period for the development of single-cell sequencing, with scRNA-seq undergoing a tremendous leap in sensitivity and throughput. [...] Read more.
Single-cell RNA sequencing (scRNA-seq) technology provides a powerful tool for understanding complex biosystems at the single-cell and single-molecule level. The past decade has been a golden period for the development of single-cell sequencing, with scRNA-seq undergoing a tremendous leap in sensitivity and throughput. The application of droplet- and microwell-based microfluidics in scRNA-seq has contributed greatly to improving sequencing throughput. This review introduces the history of development and important technical factors of scRNA-seq. We mainly focus on the role of microfluidics in facilitating the development of scRNA-seq technology. To end, we discuss the future directions for scRNA-seq. Full article
(This article belongs to the Special Issue Microfluidics System and Its Application in Single Cell Analysis)
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25 pages, 4793 KiB  
Review
Microfluidic Compartmentalization Platforms for Single Cell Analysis
by Xuhao Luo, Jui-Yi Chen, Marzieh Ataei and Abraham Lee
Biosensors 2022, 12(2), 58; https://doi.org/10.3390/bios12020058 - 21 Jan 2022
Cited by 15 | Viewed by 5997
Abstract
Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technology has [...] Read more.
Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technology has revolutionized single-cell analysis through precise manipulation of liquid and compartmentalizing single cells in small volumes (pico- to nano-liter). Due to its advantages from miniaturization, microfluidic systems offer an array of capabilities to study genomics, transcriptomics, and proteomics of a large number of individual cells. In this regard, microfluidic systems have emerged as a powerful technology to uncover cellular heterogeneity and expand the depth and breadth of single-cell analysis. This review will focus on recent developments of three microfluidic compartmentalization platforms (microvalve, microwell, and microdroplets) that target single-cell analysis spanning from proteomics to genomics. We also compare and contrast these three microfluidic platforms and discuss their respective advantages and disadvantages in single-cell analysis. Full article
(This article belongs to the Special Issue Microfluidics System and Its Application in Single Cell Analysis)
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