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The Immune-Endocrine Aspect of Stem Cells—a Different Diagnostic Approach to...
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The Immune-Endocrine Aspect of Stem Cells—a Different Diagnostic Approach to Diseases 2023

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Pathology and Molecular Diagnostics".

Deadline for manuscript submissions: closed (31 July 2024) | Viewed by 3714

Special Issue Editor

Dr. Ciro Gargiulo Isacco

E-Mail Website
Guest Editor
Department of Interdisciplinary Medicine, Section of Microbiology and Virology, School of Medicine, University of Bari “Aldo Moro”, 70121 Bari, Italy
Interests: immunology; regenerative medicine; microbiology; virology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are pleased to invite you to submit valuable manuscripts and papers to this Special Issue.

The immune-endocrine and stem cells represent not only a promising diagnostic stance but also an innovative therapeutic approach in several conditions. Numerous preclinical and clinical works suggest that a broader approach is required for complete success in inflammatory, metabolic, and degenerative disorders. In a disease such as diabetes, bone decay, cardiovascular conditions and neuro-degenerative conditions some of the strategies explored to reverse or halt beta-cells, osteoblasts and neuron destruction the support of anti-inflammatory therapies, hormones and stem cell-based therapies may represent a new way out. A multi-disciplinary approach and the increasing number of proofs suggest that genetic susceptibility (presence of SNPs), hormone deficit, immune instability and aging play a significant role in triggering and sustaining generalized inflammation in degenerative-metabolic type diseases such as type 2 diabetes, osteoporosis, and dementia and so on. Moreover, the use of stem cells, especially mesenchymal stem cells (MSCs) due to their crucial immunomodulation activity could be extremely important in re-organizing and resetting damaged tissues and cells.

This Special Issue of Diagnostics is devoted to providing renewed diagnostic/therapy tools that are the foundation of contemporary personalized medicine. We, therefore, invite review manuscripts as well as original manuscripts dealing with the identification of novel endocrine-immune-regenerative based approaches/strategies in metabolic-inflammatory-degenerative diseases. The focus could be either on diagnosis, for instance, the screenings of specific genetic patterns (SNPs), or therapeutics,  such as the use of hormone therapy, the use of anti-inflammatory cytokines-based therapy, the use of biomaterials, stem cell treatment or both.

In this Special Issue, original research articles and reviews are welcome.

Research areas may include (but are not limited to) the following: hormones and immunity (bio-identical hormones), regenerative therapy, and stem cell use in viral infection….

I look forward to receiving your contributions.

Dr. Ciro Gargiulo Isacco
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • stem cells
  • bio-identical hormones
  • metabolic-inflammatory disorders
  • SNPs
  • neuro-degenerative disorders

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Published Papers (1 paper)

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Research

16 pages, 2512 KiB  
Article
Multiplex RT Real-Time PCR Based on Target Failure to Detect and Identify Different Variants of SARS-CoV-2: A Feasible Method That Can Be Applied in Clinical Laboratories
by Van Hung Pham, Huong Thien Pham, Mario G. Balzanelli, Pietro Distratis, Rita Lazzaro, Quoc Viet Nguyen, Viet Quoc Tran, Duy Khanh Tran, Luan Duy Phan, Sang Minh Pham, Binh Thai Pham, Chien Vo Duc, Ha Minh Nguyen, Dung Ngoc Thi Nguyen, Ngoc Van Tran, Son Truong Pham, Camelia Queck, Kieu Diem Cao Nguyen, Francesco Inchingolo, Raffaele Del Prete, Nam Hai Dinh Nguyen, Luigi Santacroce and Ciro Gargiulo Isaccoadd Show full author list remove Hide full author list
Diagnostics 2023, 13(8), 1364; https://doi.org/10.3390/diagnostics13081364 - 7 Apr 2023
Cited by 1 | Viewed by 3209
Abstract
Shortly after its emergence, Omicron and its sub-variants have quickly replaced the Delta variant during the current COVID-19 outbreaks in Vietnam and around the world. To enable the rapid and timely detection of existing and future variants for epidemiological surveillance and diagnostic applications, [...] Read more.
Shortly after its emergence, Omicron and its sub-variants have quickly replaced the Delta variant during the current COVID-19 outbreaks in Vietnam and around the world. To enable the rapid and timely detection of existing and future variants for epidemiological surveillance and diagnostic applications, a robust, economical real-time PCR method that can specifically and sensitively detect and identify multiple different circulating variants is needed. The principle of target- failure (TF) real-time PCR is simple. If a target contains a deletion mutation, then there is a mismatch with the primer or probe, and the real-time PCR will fail to amplify the target. In this study, we designed and evaluated a novel multiplex RT real-time PCR (MPL RT-rPCR) based on the principle of target failure to detect and identify different variants of SARS-CoV-2 directly from the nasopharyngeal swabs collected from COVID-19 suspected cases. The primers and probes were designed based on the specific deletion mutations of current circulating variants. To evaluate the results from the MPL RT-rPCR, this study also designed nine pairs of primers for amplifying and sequencing of nine fragments from the S gene containing mutations of known variants. We demonstrated that (i) our MPL RT-rPCR was able to accurately detect multiple variants that existed in a single sample; (ii) the limit of detection of the MPL RT-rPCR in the detection of the variants ranged from 1 to 10 copies for Omicron BA.2 and BA.5, and from 10 to 100 copies for Delta, Omicron BA.1, recombination of BA.1 and BA.2, and BA.4; (iii) between January and September 2022, Omicron BA.1 emerged and co-existed with the Delta variant during the early period, both of which were rapidly replaced by Omicron BA.2, and this was followed by Omicron BA.5 as the dominant variant toward the later period. Our results showed that SARS-CoV-2 variants rapidly evolved within a short period of time, proving the importance of a robust, economical, and easy-to-access method not just for epidemiological surveillance but also for diagnoses around the world where SARS-CoV-2 variants remain the WHO’s highest health concern. Our highly sensitive and specific MPL RT-rPCR is considered suitable for further implementation in many laboratories, especially in developing countries. Full article
(This article belongs to the Special Issue The Immune-Endocrine Aspect of Stem Cells—a Different Diagnostic Approach to Diseases 2023)
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