Strategies for Controlling Pathogenic Microbial Contamination in Animal-Derived Food Products: An Antimicrobial Approach

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: 15 February 2025 | Viewed by 2595

Special Issue Editor

College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China
Interests: resistance mechanisms; epidemiological distribution; foodborne pathogens; evolution of antibiotic resistance

Special Issue Information

Dear Colleagues,

Foodborne pathogenic microorganisms, causing various foodborne diseases, can be found in animal-derived foods, manufacturing process of food products and animal products. Antibiotic resistance in foodborne bacteria is serious and poses a serious threat to the food chain safety. Therefore, it is necessary to study the prevalence of antibiotic-resistant foodborne bacteria, and rapid detection methods and strategies (such as bacteriophage or essential oils) for controlling foodborne pathogenic microorganisms in animals and their products. We cordially invite scientists, researchers and other food professionals to submit original reviews/research articles related to the prevalence of antibiotic-resistant foodborne pathogens or strategies for controlling foodborne pathogens to this Special Issue.

Dr. Baotao Liu
Guest Editor

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Keywords

  • antibiotic resistance
  • foodborne pathogens
  • food animals
  • animal products
  • prevention
  • control

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Published Papers (2 papers)

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Research

14 pages, 2676 KiB  
Article
Concurrence of Inactivation Enzyme-Encoding Genes tet(X), blaEBR, and estT in Empedobacter Species from Chickens and Surrounding Environments
by Chong Chen, Yilin Lv, Taotao Wu, Jing Liu, Yanan Guo and Jinlin Huang
Foods 2024, 13(19), 3201; https://doi.org/10.3390/foods13193201 - 9 Oct 2024
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Abstract
The emergence of inactivation enzyme-encoding genes tet(X), blaEBR, and estT challenges the effectiveness of tetracyclines, β-lactams, and macrolides. This study aims to explore the concurrence and polymorphism of their variants in Empedobacter sp. strains from food-producing animals and surrounding environments. [...] Read more.
The emergence of inactivation enzyme-encoding genes tet(X), blaEBR, and estT challenges the effectiveness of tetracyclines, β-lactams, and macrolides. This study aims to explore the concurrence and polymorphism of their variants in Empedobacter sp. strains from food-producing animals and surrounding environments. A total of eight tet(X) variants, seven blaEBR variants, and seven estT variants were detected in tet(X)-positive Empedobacter sp. strains (6.7%) from chickens, sewage, and soil, including 31 Empedobacter stercoris and 6 novel species of Taxon 1. All of them were resistant to tigecycline, tetracycline, colistin, and ciprofloxacin, and 16.2% were resistant to meropenem, florfenicol, and cefotaxime. The MIC90 of tylosin, tilmicosin, and tildipirosin was 128 mg/L, 16 mg/L, and 8 mg/L, respectively. Cloning expression confirmed that tet(X6) and the novel variants tet(X23), tet(X24), tet(X25), tet(X26), and tet(X26.2) conferred high-level tigecycline resistance, while all of the others exhibited relatively low-level activities or were inactivated. The bacterial relationship was diverse, but the genetic environments of tet(X) and blaEBR were more conserved than estT. An ISCR2-mediated tet(X6) transposition structure, homologous to those of Acinetobacter sp., Proteus sp., and Providencia sp., was also identified in Taxon 1. Therefore, the tet(X)-positive Empedobacter sp. strains may be ignored and pose a serious threat to food safety and public health. Full article
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19 pages, 2915 KiB  
Article
Isolation and Characterization of Two Novel Lytic Bacteriophages against Salmonella typhimurium and Their Biocontrol Potential in Food Products
by Yaxiong Song, Wentao Gu, Yaozhong Hu, Bowei Zhang, Jin Wang, Yi Sun, Wenhui Fu, Xinyang Li, Xiaolong Xing and Shuo Wang
Foods 2024, 13(19), 3103; https://doi.org/10.3390/foods13193103 - 28 Sep 2024
Viewed by 918
Abstract
Foodborne pathogens, such as Salmonella, are major factors that pose significant threats to global food safety and public health. Salmonella typhimurium is a prominent serotype contributing to non-typhoidal salmonellosis, which is a prevalent foodborne illness affecting humans and animals. Bacteriophages are considered [...] Read more.
Foodborne pathogens, such as Salmonella, are major factors that pose significant threats to global food safety and public health. Salmonella typhimurium is a prominent serotype contributing to non-typhoidal salmonellosis, which is a prevalent foodborne illness affecting humans and animals. Bacteriophages are considered one of the most environmentally friendly biocontrol agents, particularly in the food industry, owing to their high specificity and high safety. However, the emergency of phage-resistant mutants limits the biocontrol effect of phage treatment, leading to the requirement for a high diversity of lytic phages. Therefore, the study isolated and characterized two novel lytic Salmonella bacteriophages (SPYS_1 and SPYS_2) targeting S. typhimurium ATCC14028 and evaluated their effectiveness in reducing the contamination rates for milk and chicken tenders. Morphological and genomic analyses indicated that Salmonella phages SPYS_1 and SPYS_2 are novel species classified under the genus Skatevirus and the genus Berlinvirus, respectively. Both phages exhibited high stability across a broad range of thermal and pH conditions. The one-step growth curve result suggested that both phages had a short adsorption time and a large burst size in a single lytic cycle. The phage SPYS_1 demonstrated a noteworthy inhibition effect on the growth of S. typhimurium ATCC14028 in milk, resulting in a ~2-log reduction within the 2 to 4 h range. Overall, both phages have shown significant potential for application in food safety in the future. Full article
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