Submit to Forests Review for Forests Propose a Special Issue

Journal Menu

Journal Browser

Application of Biotechnology Techniques on Tree Species

Print Special Issue Flyer
Special Issue Editors
Special Issue Information
Keywords
Benefits of Publishing in a Special Issue
Published Papers

A special issue of Forests (ISSN 1999-4907). This special issue belongs to the section "Genetics and Molecular Biology".

Deadline for manuscript submissions: closed (25 February 2022) | Viewed by 36369

Share This Special Issue

Special Issue Editors

Dr. Jorge Canhoto

E-Mail Website
Guest Editor

Department of Life Sciences, Centre for Functional Ecology, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal
Interests: plant breeding; plant biotechnology; in vitro culture; genomics; plant anatomy; somatic embryogenesisinterests; plant development; forestry
Special Issues, Collections and Topics in MDPI journals

Dr. Paloma Moncaleán

E-Mail
Guest Editor

Department of Forestry Science, NEIKER-BRTA, 01080 Arkaute, Spain
Interests: bioeconomy; in vitro culture; physiology; forestry
Special Issues, Collections and Topics in MDPI journals

Dr. Sandra Correia

E-Mail Website
Guest Editor

Department of Life Sciences, Centre for Functional Ecology, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal
Interests: plant biotechnology; plant molecular biology; plant stress biology; proteomics; transcriptomics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Trees are dominant species in many land ecosystems and their economic relevance is well known, not only for the production of wood and other products, but also because they deliver a variety of ecosystem services, including temperature regulation, mitigation of soil erosion, and managing and filtering rainwater, and are habitats for other organisms. Due to their long reproductive and life cycles, trees require specific strategies for breeding and large-scale propagation. In recent years, biotechnology is assuming an increasingly important role in tree breeding and cloning, through the application of techniques such as somatic embryogenesis, propagation in bioreactors, genetic transformation, proteomics, genomics, and production of synthetic seeds, among many others. Based on these tools, improved trees displaying new features are now in the field assuring higher productivities and helping to preserve natural forests while contributing to fix CO₂ and to avoid desertification, both from an ecological and human perspective. This Special Issue will keep researchers and other stakeholders on the cutting edge of the latest developments in the field of tree biotechnology. Those interested in tree biotechnology are welcome to collaborate and share their more recent results in this field.

Prof. Dr. Jorge Canhoto
Dr. Paloma Moncaleán
Dr. Sandra Correia
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Forests is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

acclimatization
bioreactors
breeding
cloning
genetic transformation
in vitro
molecular biology
omics
rooting

Benefits of Publishing in a Special Issue

Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (11 papers)

Download All Papers

Order results

Result details

Show export options Show export options

Select all

Export citation of selected articles as:

Research

15 pages, 1633 KiB

Open AccessArticle

Somatic Embryogenesis of Pinus sylvestris L. from Parent Genotypes with High- and Low Stilbene Content in Their Heartwood

by Anni Harju, Susanne Heiska, Riitta Julkunen-Tiitto, Martti Venäläinen and Tuija Aronen

Forests 2022, 13(4), 557; https://doi.org/10.3390/f13040557 - 31 Mar 2022

Cited by 3 | Viewed by 2505

Abstract

The increasing concern about ecological impacts of wood preservation chemicals has raised the interest in the natural durability of Scots pine (Pinus sylvestris L.) heartwood. Phenolic compounds—stilbenes—have been found to inhibit fungal growth, making heartwood more resistant to decay. There is a strong genetic component in the stilbene content of the heartwood in Scots pine, with a positive correlation between stilbene content in the heartwood of mother trees and their progenies. Vegetative propagation, i.e., somatic embryogenesis (SE) of Scots pine genotypes with high content of stilbenes could provide a way to produce more durable timber, assuming that there is no trade-off between SE propagation and capacity for high stilbene synthesis. To study this, we made SE initiations from parent genotypes with high and low content of stilbenes in their heartwood, using seed embryos from both open-pollinations and controlled crossings as explants. The success of SE was followed from initiation to embling acclimatization, together with measurements of stilbene content in the explants and the established SE lines. The results show that SE can be induced and emblings regenerated from trees with both high and low content of stilbene. Content of stilbenes was generally low in SE cultures and varied widely among the lines. Following the successful initiation, the later phases of SE propagation proceeded with no connection to the parent genotypes or the stilbene level of the ECs and had large variation among SE-lines. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Graphical abstract

13 pages, 1881 KiB

Open AccessArticle

Variation of Morphological Traits and Quality Indices of Micropropagated Melia volkensii Gürke Clones before Field Planting

by Constantin Dushimimana, Titus Magomere, Jackson Mulatya, Jan Vandenabeele, Florence Olubayo, Guy Smagghe and Stefaan P. O. Werbrouck

Forests 2022, 13(2), 337; https://doi.org/10.3390/f13020337 - 18 Feb 2022

Cited by 9 | Viewed by 3442

Abstract

The quality of acclimatized in vitro cultivated plants is essential to ensure good survival and growth after planting in field conditions. After two months of acclimatization, this study revealed a significant variation in survival rate, shoot characteristics, root traits, and biomass features between 13 clones of Melia volkensii Gürke. A number of quality indices such as the Dickson Quality Index (DQI), shoot dry weight: root dry weight ratio (S:R), and sturdiness quotient (SQ) also showed a large variation. The survival rate was genotype-dependent, with an average of 85%. Extreme genotypes were tall, had long internodes, a lot of leaves, and a large leaf area. At the other side of the spectrum, there were small clones with short internodes and a reduced number of leaves and leaf areas. The high S:R values (>2) of the evaluated clones indicate an imbalance between the shoot and root system, negatively influencing the quality of the in vitro produced M. volkensii plants. The SQ for all clones was below the threshold value of 6, implying a good and expected survival rate. This study illustrates that some clones might systematically score better for SQ, S:R, plant volume, and DQI after acclimatization and that these factors could form the basis for selection. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Figure 1

16 pages, 14528 KiB

Open AccessArticle

Differences in Environmental and Hormonal Regulation of Growth Responses in Two Highly Productive Hybrid Populus Genotypes

by Jonas Žiauka, Greta Striganavičiūtė, Iwona Szyp-Borowska, Sigutė Kuusienė and Marzena Niemczyk

Forests 2022, 13(2), 183; https://doi.org/10.3390/f13020183 - 26 Jan 2022

Viewed by 2380

Abstract

Phenotypic plasticity, in response to adverse conditions, determines plant productivity and survival. The aim of this study was to test if two highly productive Populus genotypes, characterised by different in vitro etiolation patterns, differ also in their responses to hormones gibberellin (GA) and abscisic acid (ABA), and to a GA biosynthesis inhibitor paclobutrazol (PBZ). The experiments on shoot cultures of ‘Hybrida 275′ (abbr. H275; Populus maximowiczii × P. trichocarpa) and IBL 91/78 (Populus tremula × P. alba) were conducted by either modulating the physical in vitro environment or by adding specific chemicals to the nutrient medium. Our results revealed two main sets of differences between the studied genotypes in environmental and hormonal regulation of growth responses. First, the genotype H275 responded to darkness with PBZ-inhibitable shoot elongation; in contrast, the elongation of IBL 91/78 shoots was not affected either by darkness or PBZ treatment. Secondly, the explants of H275 were unable to recover their growth if it was inhibited with ABA; in contrast, those of IBL 91/78 recovered so well after the temporal inhibition by ABA that, when rooted subsequently, they developed longer shoots and roots than without a previous ABA treatment. Our results indicate that GA catabolism and repressive signalling provide an important pathway to control growth and physiological adaptation in response to immediate or impending adverse conditions. These observations can help breeders define robust criteria for identifying genotypes with high resistance and productivity and highlight where genotypes exhibit susceptibility to stress. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Figure 1

23 pages, 30169 KiB

Open AccessArticle

Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees

by Mª Teresa Martínez, Isabel Arrillaga, Ester Sales, María Amparo Pérez-Oliver, Mª del Carmen González-Mas and Elena Corredoira

Forests 2021, 12(12), 1634; https://doi.org/10.3390/f12121634 - 25 Nov 2021

Cited by 14 | Viewed by 3215

Abstract

Holm oak populations have deteriorated drastically due to oak decline syndrome. The first objective of the present study was to investigate the use of axillary budding and somatic embryogenesis (SE) to propagate asymptomatic holm oak genotypes identified in disease hotspots in Spain. Axillary budding was achieved in two out of six tolerant genotypes from the south-western region and in two out of four genotypes from the Mediterranean region. Rooting of shoots cultured on medium supplemented with 3 mg L⁻¹ of indole-3-acetic acid plus 0.1 mg L⁻¹ α-naphthalene acetic acid was achieved, with rates ranging from 8 to 36%. Shoot cultures remained viable after cold storage for 9–12 months; this procedure is therefore suitable for medium-term conservation of holm oak germplasm. SE was induced in two out of the three genotypes tested, by using nodes and shoot tips cultured in medium without plant growth regulators. In vitro cloned progenies of the tolerant genotypes PL-T2 and VA5 inhibited growth of Phytophthora cinnamomi mycelia when exposed to the oomycete in vitro. Significant differences in total phenol contents and in the expression profiles of genes regulating phenylpropanoid biosynthesis were observed between in vitro cultured shoots derived from tolerant trees and cultures established from control genotypes. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Figure 1

12 pages, 3513 KiB

Open AccessArticle

Plant Regeneration and In Vitro Growth Performance of Male-Sterile Somatic Plantlets of Sugi (Japanese Cedar, Cryptomeria japonica) Derived from Different Embryogenic Cell Lines

by Momi Tsuruta, Tsuyoshi E. Maruyama, Saneyoshi Ueno, Takumi Kaneeda and Yoshinari Moriguchi

Forests 2021, 12(11), 1592; https://doi.org/10.3390/f12111592 - 18 Nov 2021

Cited by 1 | Viewed by 2185

Abstract

With the spread of pollinosis caused by sugi (Japanese cedar, Cryptomeria japonica) pollen, the use of pollen-free somatic seedlings of sugi is expected in Japan. However, there is a lack of knowledge on the relationship between the abilities during somatic embryogenesis, initial in vitro growth traits, and subsequent growth of somatic seedlings. In the present study, we provide the first basic information on somatic embryo maturation efficiency, somatic embryo germination, and plantlet conversion frequencies, as well as on in vitro growth performance of pollen-free somatic plantlets derived from different embryogenic cell lines (ECLs). Somatic embryo maturation efficiency varied from 34 to 514 cotyledonary embryos per plate and the average for the 19 ECLs tested was 244 embryos per plate. Subsequently, the overall average rates of somatic embryo germination and conversion among ECLs were 87.8% and 85.3%, respectively. The results of in vitro growth performance of pollen-free somatic plantlets showed significant differences in growth rate among ECLs. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Graphical abstract

15 pages, 4290 KiB

Open AccessArticle

The Effect of Sucrose Supplementation on the Micropropagation of Salix viminalis L. Shoots in Semisolid Medium and Temporary Immersion Bioreactors

by Diego Gago, Saladina Vilavert, María Ángeles Bernal, Conchi Sánchez, Anxela Aldrey and Nieves Vidal

Forests 2021, 12(10), 1408; https://doi.org/10.3390/f12101408 - 15 Oct 2021

Cited by 16 | Viewed by 2874

Abstract

The effect of sucrose concentration on the micropropagation of axillary shoots of willow was investigated. The following factors were examined: the culture system (semisolid medium in glass jars versus liquid medium in temporary immersion bioreactors), the type of explant (apical and basal sections), the frequency of immersion, and CO₂ enrichment. Shoots and leaf growth were significantly higher in RITA^® bioreactors than in the jars for all the sucrose treatments. Apical or basal sections of willow cultured in bioreactors under high light intensity (150 µmol m⁻² s⁻¹) and ventilated six times a day with CO₂-enriched air were successfully proliferated without sucrose, whereas shoots cultured in jars did not proliferate well if sucrose concentration was 0.5% or lower. More roots were formed when sucrose was added to the medium. Shoots cultured in bioreactors were successfully acclimatized irrespective of the sucrose treatment and the root biomass when transferred to ex vitro conditions. This is the first report of photoautotrophic willow micropropagation, our results confirm the importance of proper gaseous exchange to attain autotrophy during in vitro propagation. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Graphical abstract

14 pages, 3200 KiB

Open AccessArticle

Explant, Medium, and Plant Growth Regulator (PGR) Affect Induction and Proliferation of Callus in Abies koreana

by Ge Guo and Byoung Ryong Jeong

Forests 2021, 12(10), 1388; https://doi.org/10.3390/f12101388 - 11 Oct 2021

Cited by 13 | Viewed by 3413

Abstract

Korean fir (Abies koreana E.H. Wilson) is a unique Pinaceae tree species endemic in Korea. In recent years, it is believed that climate change has caused many of them to die. Therefore, it has become extremely important to protect and preserve this tree species. In this study, the possibility of callus induction using different explants, media, and plant growth regulators (PGRs) was studied. After the dormancy period in May 2020, needles and stem segments that grew from the leaf buds as the explants were collected from one-year-old shoots. The explants were disinfected and subsequently transferred to culture media supplemented with different combinations of auxins and cytokinins. These explants were cultured in the dark in a culture room with a 16 h photoperiod, day/night temperature of 24/18 °C, and 80% relative humidity. After 8 weeks, significant differences were observed in the callus induction and proliferation, as affected by the explant type, basic medium, and PGR. The stem segments were more suitable as the explants for callus induction than needles were. Furthermore, fluffy calli suitable for differentiating the regeneration buds were observed on the calli induced from stem segments. The Murashige and Skoog (MS) medium was the most effective of the three media used in this study, namely MS, Douglas fir cotyledon revised (DCR), and Quoirin and Lepoivre (LP) media, with the highest callus induction ratio of stem segments being 100.0%. The highest fresh callus weight was also observed on the MS medium (819.3 mg). Moreover, the PGR combinations of α-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and 6-benzylaminopurine (6-BA) consistently exerted a positive influence on callus induction throughout this study. In addition, the advantages of these two kinds of PGR were reflected in callus proliferation. The callus proliferation ratio reached 1147.6% as compared to the initial fresh weight, with a high concentration of 2,4-D (3.0 mg·L⁻¹). In conclusion, the MS medium was optimal for callus induction on the stem segment explants, and 2,4-D promoted callus induction as well as an increased proliferation ratio of callus in A. koreana. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Figure 1

18 pages, 31382 KiB

Open AccessArticle

Experimental Examination of Vegetative Propagation Methods of Nothofagus antarctica (G. Forst.) Oerst. for Restoration of Fire-Damaged Forest in Torres del Paine National Park, Chile

by Josef Cafourek, Petr Maděra, Josef Střítecký, Radim Adolt and Martin Smola

Forests 2021, 12(9), 1238; https://doi.org/10.3390/f12091238 - 13 Sep 2021

Cited by 3 | Viewed by 3407

Abstract

Nothofagus antarctica (Antarctic beech) is one of the main woody plants in the temperate deciduous forests and anti-boreal forests of the southern hemisphere. Since colonization of the Andean-Patagonian region by European settlers, however, stands of this species have been severely affected by fires caused by human activities, considerably reducing their area. To restore these forests to their area occupied before the fires, it is necessary to use artificial regeneration, relying on production of transplants in forest nurseries. Due to the low capacity for seed propagation, we focus on possibilities of producing seedlings by vegetative propagation. In a trial, we collected cuttings during three sets of dates, and attempted to root them using three combinations of substrate and ten combinations of stimulators. Using the most favorable combination of collection period, substrate and stimulator tested resulted in rooting of 23% of the cuttings, which exceeds the documented germination rates for this species. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Graphical abstract

15 pages, 9491 KiB

Open AccessArticle

Regeneration of Pinus halepensis (Mill.) through Organogenesis from Apical Shoot Buds

by Cátia Pereira, Itziar A. Montalbán, Ana Pedrosa, Jéssica Tavares, Alexey Pestryakov, Nina Bogdanchikova, Jorge Canhoto and Paloma Moncaleán

Forests 2021, 12(3), 363; https://doi.org/10.3390/f12030363 - 19 Mar 2021

Cited by 9 | Viewed by 3322

Abstract

Organogenesis and somatic embryogenesis have been widely applied as the two main regeneration pathways in plant tissue cultures. However, recalcitrance is still the main restriction in the clonal propagation of many woody species, especially in conifers. They undergo a “phase change” that leads to significant loss of vegetative propagation capacity, reducing the aptitude of tissues and organs to be regenerated in vitro beyond this point. In line with this, the in vitro regeneration of mature conifer trees has been a long-cherished goal in many laboratories worldwide. Based on previous works in Pinus species regeneration from adult trees, we now present data about the culture of apical shoot buds in an attempt to induce organogenesis and somatic embryogenesis to clone mature trees of Aleppo pine (Pinus halepensis). Reinvigorated axillary shoots were submitted to conditions usually applied to induce somatic embryogenesis through the manipulation of culture media, including the use of auxins such as 2,4-Dichlorophenoxyacetic acid and 1-Naphthaleneacetic acid, cytokinins (6-benzyladenine and kinetin), and phytosulfokine (50, 100, and 200 nM). Although somatic embryos could not be obtained, an embryogenic-like tissue was produced, followed by the emergence of actively proliferating non-embryogenic calli. Variations in the consistence, texture, and color of non-embryogenic calli were observed; especially those arising in the media containing phytosulfokine. Reinvigorated shoots, induced by 22 or 44 µM 6-benzyladenine, were obtained through organogenesis and acclimatized, and phenotypically normal plants were obtained. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Figure 1

17 pages, 11776 KiB

Open AccessArticle

Hybridization Assays in Strawberry Tree toward the Identification of Plants Displaying Increased Drought Tolerance

by João Martins, Pedro Monteiro, Glória Pinto and Jorge Canhoto

Forests 2021, 12(2), 148; https://doi.org/10.3390/f12020148 - 27 Jan 2021

Cited by 7 | Viewed by 2906

Abstract

Arbutus unedo L. is a small Ericaceae tree with a circum-Mediterranean distribution. It has a huge ecological impact on southern Europe forests and a great economic importance as a source of phytochemicals with bioactive properties and for fruit production. On the foreseen climate change context, breeding toward drought tolerance is necessary in order to ameliorate plant performance. Therefore, the aim of this work was to study the reproduction mechanisms of the strawberry tree, obtain new genetic combinations by hybridization, and select genotypes more tolerant to drought stress. A morphological analysis of flowers and pollen was carried out, and controlled pollinations were performed both in vitro and ex vitro. The very first approach on strawberry tree breeding by means of hybridization is also presented. Several physiological parameters were evaluated on 26 genotypes submitted to a water-deficit regime. Plant behavior under drought greatly varied among genotypes, which showed high phenotype plasticity. Three genotypes that were able to cope with water restriction without compromising net CO₂ assimilation were identified as highly tolerant to drought stress. The results obtained elucidate the reproduction mechanisms of the strawberry tree and open the way for a long-term breeding program based on the selection of drought-tolerant plants. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Graphical abstract

12 pages, 1633 KiB

Open AccessArticle

Hybrid Pine (Pinus attenuata × Pinus radiata) Somatic Embryogenesis: What Do You Prefer, Mother or Nurse?

by Itziar A. Montalbán, Ander Castander-Olarieta, Cathy L. Hargreaves, Keiko Gough, Cathie B. Reeves, Shaf van Ballekom, Tomás Goicoa, María Dolores Ugarte and Paloma Moncaleán

Forests 2021, 12(1), 45; https://doi.org/10.3390/f12010045 - 31 Dec 2020

Cited by 6 | Viewed by 3297

Abstract

Development of hybrid pines of Pinus radiata D. Don for commercial forestry presents an opportunity to diversify the current resource of plant material. Climate change and different land uses pose challenges, making alternative species necessary to guarantee wood and non-wood products in the future. Pinus radiata var. cedrosensis × Pinus attenuata hybrid possesses different attributes, such as tolerance to drought conditions, better growth and resistance to snow damage at higher altitudes, and more importantly, different wood quality characteristics. Embryogenic cell lines were successfully initiated reciprocal hybrids using as initial explants megagametophytes, excised zygotic embryos and excised zygotic embryos plus nurse culture. However, the questions raised were: does the initiation environment affect the conversion to somatic plantlets months later? Does the mother tree or the cross have an effect on the conversion to somatic plantlets? In the present work we analysed the maturation rate, number of somatic embryos, germination rate, and the ex-vitro growth in cell lines derived from different initiation treatments, mother tree species, and crosses. Differences were not observed for in vitro parameters such as maturation and germination. However, significant differences were observed due to the mother tree species in relation with the ex-vitro growth rates observed, being higher those in which P. radiata acted as a mother. Moreover, embryogenic cell lines from these hybrids were stored at −80 °C and regenerated after one and five years. Full article

(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)

► Show Figures

Journal Menu

Journal Browser

Application of Biotechnology Techniques on Tree Species

Share This Special Issue

Special Issue Editors

Special Issue Information

Keywords

Benefits of Publishing in a Special Issue

Published Papers (11 papers)

Research

Further Information

Guidelines

MDPI Initiatives

Follow MDPI