Safety of Genetically Modified Crops and Plant Functional Genomics

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 20 January 2025 | Viewed by 1203

Special Issue Editors

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: molecular breeding; genetics; transcriptome; safety of genetically modified crops

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Guest Editor
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Interests: molecular breeding; transcriptome; fatty acid metabolism; miRNA

Special Issue Information

Dear Colleagues,

Since GM crops began to be grown commercially in 1996, remarkable economic and social benefits have been achieved. In recent years, the development and approval of GM crops and plants have significantly increased, and the crops have been released for commercial cultivation across the globe. This phenomenon is also associated with developments in genetic modification techniques such as genome editing, CRISPR, transcription-activator-like effector nuclease (TALEN), zinc-finger nucleases (ZNFs), RNA-directed DNA methylation, cisgenesis and intragenesis, RNA-editing, and gene transfer via gene gun or agrobacterium-mediated transformation. Currently, there is also a debate about the safety of GM plants.

For this Special Issue, we invite researchers to submit articles, reviews, opinions, and short communications, including functional genomics tools and approaches being developed and deployed to provide new options for advancing the breeding of next-generation crops. Studies that involve the development, risk assessment, and regulation of GM plants.

Dr. Xinwu Pei
Dr. Lu Gan
Guest Editors

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Keywords

  • genetically modified plants
  • GM regulation
  • GM risk assessment
  • functional genomics
  • genetic improvement

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Published Papers (1 paper)

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Research

15 pages, 1696 KiB  
Article
An Integrated Strategy for Analyzing the Complete Complex Integrated Structure of Maize MON810 and Identification of an SNP in External Insertion Sequences
by Chunmeng Huang, Yongjun Zhang, Huilin Yu, Xiuping Chen and Jiajian Xie
Plants 2024, 13(16), 2276; https://doi.org/10.3390/plants13162276 - 15 Aug 2024
Viewed by 837
Abstract
Genetically modified maize (Zea mays L.) MON810 was approved for importation into China for feed use in 2004; however, the localization data concerning exogenous insertion sequences, which confer insect resistance, have been questionable. MON810 maize plants discovered in northeastern China were used [...] Read more.
Genetically modified maize (Zea mays L.) MON810 was approved for importation into China for feed use in 2004; however, the localization data concerning exogenous insertion sequences, which confer insect resistance, have been questionable. MON810 maize plants discovered in northeastern China were used to analyze the molecular characteristics of the exogenous insertion. Using PacBio-HiFi sequencing and PCR assays, we found the insertion was located in chromosome 8, and there was a CaMV35S promoter, hsp70 intron, and insecticide gene cry1Ab, except for genome sequence insertion in the MON810 event. Importantly, the 5′ and 3′ flanking sequences were located in the region of 55869747–55879326 and 68416240–68419152 on chromosome 5, respectively. The results of this study correct previous results on the genomic localization of the insertion structure for the MON810 event. We also found a single-nucleotide polymorphism (SNP) in the hsp70 intron, which is most likely the first SNP found in a transgenic insertion sequence. PCR amplification in conjunction with Sanger sequencing, allele-specific PCR (AS-PCR), and blocker displacement amplification (BDA) assays were all effective at detecting the base variance. The integrated strategy used in this study can serve as a model for other cases when facing similar challenges involving partially characterized genetic modification events or SNPs. Full article
(This article belongs to the Special Issue Safety of Genetically Modified Crops and Plant Functional Genomics)
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