Advances in Molecular Genetics and Breeding of Brassica napus L.

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: 20 December 2024 | Viewed by 2015

Special Issue Editors


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Guest Editor
National Institute of Agricultural Sciences, Rural Development Administration, Jeonju-si, Jeollabuk-do, Republic of Korea
Interests: rapeseed (Brassica napus L.); plant breeding; interspecific hybridization; plant biotechnology; premature germination

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Guest Editor
National Institute of Agricultural Sciences, Rural Development Administration, Jeonju-si, Jeollabuk-do, Republic of Korea
Interests: Brassica sp.; functional genomics; plant physiology; plant genetics; plant metabolism; molecular markers

Special Issue Information

Dear Colleagues,

Rapeseed (Brassica napus L.) holds the position of being the third-largest oilseed crop globally, following soybean and oil palm. It is esteemed for its production of high-quality edible oil and biofuel. The increasing demand for rapeseed across diverse industries underscores the need for continuous improvements in genetic characteristics to align with market needs. Agricultural researchers have consistently prioritized the enhancement of crop traits, focusing on both quantitative and qualitative aspects. Hence, the primary objective of this Special Issue is to compile the most recent advancements in rapeseed genetics and breeding pertaining to the mentioned areas. This includes but not restricted to exploring and defining genes/QTLs and new/highly effective alleles through functional genomics, genetics, resequencing, and breeding techniques. The aim is to enhance rapeseed oil content, improve oil quality, boost yield potential, elevate nutritional values, and fortify resilience against biotic and abiotic stresses.

Dr. Soo-In Sohn
Dr. Subramani Pandian
Guest Editors

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Keywords

  • Brassica napus L.
  • functional genomics
  • genetics
  • breeding
  • biotechnology
  • genomics
  • crop breeding
  • germplasm resources

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Published Papers (2 papers)

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Research

11 pages, 2580 KiB  
Article
Introgression of Herbicide-Resistant Gene from Genetically Modified Brassica napus L. to Brassica rapa through Backcrossing
by Subramani Pandian, Young-Sun Ban, Eun-Kyoung Shin, Senthil Kumar Thamilarasan, Muthusamy Muthusamy, Young-Ju Oh, Ho-Keun An and Soo-In Sohn
Plants 2024, 13(20), 2863; https://doi.org/10.3390/plants13202863 - 13 Oct 2024
Viewed by 638
Abstract
Interspecific hybridization between two different Brassicaceae species, namely Brassica rapa ssp. pekinensis (♀) (AA, 2n = 2x = 20) and genetically modified Brassica napus (♂) (AACC, 2n = 4x = 38), was performed to study the transmission of a herbicide resistance gene from [...] Read more.
Interspecific hybridization between two different Brassicaceae species, namely Brassica rapa ssp. pekinensis (♀) (AA, 2n = 2x = 20) and genetically modified Brassica napus (♂) (AACC, 2n = 4x = 38), was performed to study the transmission of a herbicide resistance gene from a tetraploid to a diploid Brassica species. Initially, four different GM B. napus lines were used for hybridization with B. rapa via hand pollination. Among the F1 hybrids, the cross involving the B. rapa (♀) × GM B. napus (♂) TG#39 line exhibited the highest recorded crossability index of 14.7 ± 5.7. However, subsequent backcross progenies (BC1, BC2, and BC3) displayed notably lower crossability indices. The F1 plants displayed morphological characteristics more aligned with the male parent B. napus, with significant segregation observed in the BC1 generation upon backcrossing with the recurrent parent B. rapa. By the BC2 and BC3 generations, the progeny stabilized, manifesting traits from both parents to varying degrees. Cytogenetic analysis revealed a substantial reduction in chromosome numbers, particularly in backcrossing progenies. BC1 plants typically exhibited 21–25 chromosomes, while BC2 progenies showed 21–22 chromosomes, and by the BC3 generation, stability was achieved with an average of 20 chromosomes. SSR marker analysis confirmed the progressive reduction of C-genome regions, retaining minimal C-genome-specific bands throughout successive backcrossing. Despite the extensive elimination of C-genome-specific genomic regions, the glyphosate resistance gene from the male parent B. napus was introgressed into BC3 progenies, suggesting that the glyphosate resistance gene located and introgressed in A-chromosome/genome regions of the Brassica plants. Full article
(This article belongs to the Special Issue Advances in Molecular Genetics and Breeding of Brassica napus L.)
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17 pages, 6025 KiB  
Article
Genotyping-by-Sequencing Analysis Reveals Associations between Agronomic and Oil Traits in Gamma Ray-Derived Mutant Rapeseed (Brassica napus L.)
by Woon Ji Kim, Baul Yang, Dong-Gun Kim, Sang Hoon Kim, Ye-Jin Lee, Juyoung Kim, So Hyeon Baek, Si-Yong Kang, Joon-Woo Ahn, Yu-Jin Choi, Chang-Hyu Bae, Kanivalan Iwar, Seong-Hoon Kim and Jaihyunk Ryu
Plants 2024, 13(11), 1576; https://doi.org/10.3390/plants13111576 - 6 Jun 2024
Cited by 1 | Viewed by 992
Abstract
Rapeseed (Brassica napus L.) holds significant commercial value as one of the leading oil crops, with its agronomic features and oil quality being crucial determinants. In this investigation, 73,226 single nucleotide polymorphisms (SNPs) across 95 rapeseed mutant lines induced by gamma rays, [...] Read more.
Rapeseed (Brassica napus L.) holds significant commercial value as one of the leading oil crops, with its agronomic features and oil quality being crucial determinants. In this investigation, 73,226 single nucleotide polymorphisms (SNPs) across 95 rapeseed mutant lines induced by gamma rays, alongside the original cultivar (‘Tamra’), using genotyping-by-sequencing (GBS) analysis were examined. This study encompassed gene ontology (GO) analysis and a genomewide association study (GWAS), thereby concentrating on agronomic traits (e.g., plant height, ear length, thousand-seed weight, and seed yield) and oil traits (including fatty acid composition and crude fat content). The GO analysis unveiled a multitude of genes with SNP variations associated with cellular processes, intracellular anatomical structures, and organic cyclic compound binding. Through GWAS, we detected 320 significant SNPs linked to both agronomic (104 SNPs) and oil traits (216 SNPs). Notably, two novel candidate genes, Bna.A05p02350D (SFGH) and Bna.C02p22490D (MDN1), are implicated in thousand-seed weight regulation. Additionally, Bna.C03p14350D (EXO70) and Bna.A09p05630D (PI4Kα1) emerged as novel candidate genes associated with erucic acid and crude fat content, respectively. These findings carry implications for identifying superior genotypes for the development of new cultivars. Association studies offer a cost-effective means of screening mutants and selecting elite rapeseed breeding lines, thereby enhancing the commercial viability of this pivotal oil crop. Full article
(This article belongs to the Special Issue Advances in Molecular Genetics and Breeding of Brassica napus L.)
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