In Vitro Regeneration and Clonal Propagation in Horticultural Science: Innovations and Applications

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Horticultural Science and Ornamental Plants".

Deadline for manuscript submissions: 30 April 2025 | Viewed by 1007

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Plant Molecular Biology, Faculty of Biology, Ludwig-Maximilians-University Munich, 82152 Planegg, Germany
Interests: plant molecular biology; NGS; tissue culture
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Special Issue Information

Dear Colleagues,

This Special Issue is dedicated to the discussion of the latest research and technological developments in the field of in vitro culture techniques as they pertain to horticultural plants. In vitro regeneration and propagation methods have become indispensable tools in plant biotechnology, facilitating the large-scale production of disease-free, genetically stable, and high-quality plant material. These techniques are of particular importance to the horticultural sector, which relies heavily on the propagation of superior cultivars to meet the growing demands for food, ornamental plants, and medicinal species.

The principal aim of this Special Issue is to provide a forum for researchers and industry professionals to present novel approaches to plant tissue culture, with a particular focus on the development and optimization of in vitro protocols for horticultural species. The Special Issue aims to encompass a comprehensive array of subjects, including micropropagation, somatic embryogenesis, organogenesis, synthetic seed production, and the utilization of bioreactors for large-scale propagation.

Another significant area of interest is the utilization of in vitro techniques in the improvement and conservation of plant genetic resources. In vitro regeneration protocols are frequently utilized in genetic transformation, enabling the development of plants with enhanced resistance to pests, diseases, and environmental stressors. Furthermore, tissue culture-based methods are crucial for the conservation of endangered or rare horticultural species, thus ensuring the preservation of genetic diversity.

In summary, this Special Issue aims to highlight the significant contributions of in vitro culture techniques to sustainable horticultural production and the development of novel crop varieties. It encourages interdisciplinary research and promotes knowledge exchange between academia and industry to foster innovation in horticulture.

Dr. Eslam M. Abdel-Salam
Guest Editor

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Keywords

  • horticultural crop improvement
  • in vitro propagation
  • micropropagation
  • plant tissue culture
  • somatic embryogenesis

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Published Papers (1 paper)

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Research

11 pages, 2572 KiB  
Article
Optimization of Selected Minerals and a Cytokinin for In Vitro Propagation of Little-Leaf Mockorange (Philadelphus microphyllus A. Gray) Using Response Surface Methodology (RSM)
by Razieh Khajehyar, Robert Tripepi, William J. Price and Stephen Love
Plants 2024, 13(23), 3446; https://doi.org/10.3390/plants13233446 - 9 Dec 2024
Viewed by 640
Abstract
Optimizing concentrations of minerals and phytohormones is essential when culturing a new plant species. The objective of this study was to use Response Surface Methodology (RSM) to evaluate combinations of selected minerals (N, Ca, and P) along with zeatin (Zea) to obtain optimum [...] Read more.
Optimizing concentrations of minerals and phytohormones is essential when culturing a new plant species. The objective of this study was to use Response Surface Methodology (RSM) to evaluate combinations of selected minerals (N, Ca, and P) along with zeatin (Zea) to obtain optimum shoot growth of little-leaf mockorange. Forty-six treatment combinations were assigned using Proc Optex in SAS software version 9.4. The concentrations of Zea tested were 0.82, 1.095, or 1.37 µM, and the minerals were 22.5, 30, or 37.5 mM N, 1.13, 1.5, or 1.875 mM Ca, and 0.31, 0.625, or 0.937 mM P. Treatment concentrations were tested for their effects on the number of axillary shoots formed, shoot length, and dry weight. The response surface analyses showed that the optimum concentrations of N, Ca, and P were 34 to 39 mM, 1.5 mM, and 0.625 mM, respectively. Medium supplemented with 1.1 µM Zea affected shoot growth positively. Comparison of mineral concentrations in medium with concentrations in full-strength Murashige and Skoog (MS) medium, suggests ½ MS medium should be appropriate to efficiently multiply little-leaf mockorange shoots efficiently, thus saving the time and money involved in creating a custom medium formulation. Full article
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