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Plants
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Plant Tissue Culture and Genetic Engineering
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Plant Tissue Culture and Genetic Engineering

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (30 April 2021) | Viewed by 22257

Special Issue Editor

Dr. Aung Htay Naing

E-Mail Website
Guest Editor
Department of Horticulture, Kyungpook National University, Daegu, Korea
Interests: abiotic stress; organogenesis; somatic embryogenesis; ploidy induction; genetic transformation; gene silencing; genome editing; postharvest biology; protoplast culture

Special Issue Information

Dear Colleagues,

Due to climate change and market competition, plant biologists have recently become more interested in the creation of new cultivars with novel agronomical and horticultural traits, which can resist the global climate change and increase market demand, and development of plant propagation methods for conservation of endangered species. Plant tissue culture and genetic engineering techniques have been intensively applied in the areas of plant regeneration and conservation of endangered species, crop genetic improvements using in vitro breeding techniques, and metabolic genetic engineering. This Special Issue covers various aspects of plant tissue culture and genetic engineering techniques, such as regeneration via organogenesis and somatic embryogenesis, germplasm conservation, disease elimination via meristem culture and cryopreservation, production of secondary metabolites, in vitro ploidy induction, mutagenesis, genetic transformation, genome editing, etc.

Dr. Aung Htay Naing
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Organogenesis
  • Somatic embryogenesis
  • Germplasm conservation
  • Meristem culture
  • Cryopreservation Secondary metabolites
  • In vitro ploidy induction
  • Mutagenesis
  • Genetic transformation
  • Genome editing
  • Protoplast culture
  • Anther culture

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Published Papers (4 papers)

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Research

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15 pages, 2681 KiB  
Article
Morpho-Physiological Testing of NaCl Sensitivity of Tobacco Plants Overexpressing Choline Oxidase Gene
by Galina N. Raldugina, Sergey V. Evsukov, Liliya R. Bogoutdinova, Alexander A. Gulevich and Ekaterina N. Baranova
Plants 2021, 10(6), 1102; https://doi.org/10.3390/plants10061102 - 30 May 2021
Cited by 3 | Viewed by 2749
Abstract
In this study the transgenic lines (TLs) of tobacco (Nicotianatabacum L.), which overexpress the heterologous gene encoding the bacterial enzyme choline oxidase were evaluated. The goal of our work is to study the effect of choline oxidase gene expression on the [...] Read more.
In this study the transgenic lines (TLs) of tobacco (Nicotianatabacum L.), which overexpress the heterologous gene encoding the bacterial enzyme choline oxidase were evaluated. The goal of our work is to study the effect of choline oxidase gene expression on the sensitivity of plant tissues to the action of NaCl. The regenerative capacity, rhizogenesis, the amount of photosynthetic pigments and osmotically active compounds (proline and glycine betaine) were assessed by in vitro cell culture methods using biochemical and morphological parameters. Transgenic lines with confirmed expression were characterized by high regeneration capacity from callus in the presence of 200 mmol NaCl, partial retention of viability at 400 mmol NaCl. These data correlated with the implicit response of regenerants and whole plants to the harmful effects of salinity. They turned out to be less sensitive to the presence of 200 mmol NaCl in the cultivation medium, in contrast to the WT plants. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Genetic Engineering)
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16 pages, 1899 KiB  
Article
Transcriptomic Analysis for the Identification of Metabolic Pathway Genes Related to Toluene Response in Ardisia pusilla
by Junping Xu, Chang Ho Ahn, Ju Young Shin, Pil Man Park, Hye Ryun An, Yae-Jin Kim and Su Young Lee
Plants 2021, 10(5), 1011; https://doi.org/10.3390/plants10051011 - 19 May 2021
Cited by 4 | Viewed by 3078
Abstract
Toluene is an industrial raw material and solvent that can be found abundantly in our daily life products. The amount of toluene vapor is one of the most important measurements for evaluating air quality. The evaluation of toluene scavenging ability of different plants [...] Read more.
Toluene is an industrial raw material and solvent that can be found abundantly in our daily life products. The amount of toluene vapor is one of the most important measurements for evaluating air quality. The evaluation of toluene scavenging ability of different plants has been reported, but the mechanism of plant response to toluene is only partially understood. In this study, we performed RNA sequencing (RNA-seq) analysis to detect differential gene expression in toluene-treated and untreated leaves of Ardisiapusilla. A total of 88,444 unigenes were identified by RNA-seq analysis, of which 49,623 were successfully annotated and 4101 were differentially expressed. Gene ontology analysis revealed several subcategories of genes related to toluene response, including cell part, cellular process, organelle, and metabolic processes. We mapped the main metabolic pathways of genes related to toluene response and found that the differentially expressed genes were mainly involved in glycolysis/gluconeogenesis, starch and sucrose metabolism, glycerophospholipid metabolism, carotenoid biosynthesis, phenylpropanoid biosynthesis, and flavonoid biosynthesis. In addition, 53 transcription factors belonging to 13 transcription factor families were identified. We verified 10 differentially expressed genes related to metabolic pathways using quantitative real-time PCR and found that the results of RNA-seq were positively correlated with them, indicating that the transcriptome data were reliable. This study provides insights into the metabolic pathways involved in toluene response in plants. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Genetic Engineering)
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16 pages, 5761 KiB  
Article
Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato
by Dmitry Miroshnichenko, Aleksey Firsov, Vadim Timerbaev, Oleg Kozlov, Anna Klementyeva, Lyubov Shaloiko and Sergey Dolgov
Plants 2020, 9(11), 1520; https://doi.org/10.3390/plants9111520 - 9 Nov 2020
Cited by 8 | Viewed by 4051
Abstract
Various plant-derived promoters can be used to regulate ectopic gene expression in potato. In the present study, four promoters derived from the potato genome have been characterized by the expression of identical cassettes carrying the fusion with the reporter β-glucuronidase (gusA) [...] Read more.
Various plant-derived promoters can be used to regulate ectopic gene expression in potato. In the present study, four promoters derived from the potato genome have been characterized by the expression of identical cassettes carrying the fusion with the reporter β-glucuronidase (gusA) gene. The strengths of StUbi, StGBSS, StPat, and StLhca3 promoters were compared with the conventional constitutive CaMV 35S promoter in various organs (leaves, stems, roots, and tubers) of greenhouse-grown plants. The final amount of gene product was determined at the post-transcriptional level using histochemical analysis, fluorometric measurements, and Western blot analysis. The promoter strength comparison demonstrated that the StUbi promoter generally provided a higher level of constitutive β-glucuronidase accumulation than the viral CaMV 35S promoter. Although the StLhca3 promoter was predominantly expressed in a green tissue-specific manner (leaves and stems) while StGBSS and StPat mainly provided tuber-specific activity, a “promoter leakage” was also found. However, the degree of unspecific activity depended on the particular transgenic line and tissue. According to fluorometric data, the functional activity of promoters in leaves could be arranged as follows: StLhca3 > StUbi > CaMV 35S > StPat > StGBSS (from highest to lowest). In tubers, the higher expression was detected in transgenic plants expressing StPat-gusA fusion construct, and the strength order was as follows: StPat > StGBSS > StUbi > CaMV 35S > StLhca3. The observed differences between expression patterns are discussed considering the benefits and limitations for the usage of each promoter to regulate the expression of genes in a particular potato tissue. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Genetic Engineering)
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Review

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16 pages, 659 KiB  
Review
Molecular Markers Improve Abiotic Stress Tolerance in Crops: A Review
by Adnan Younis, Fahad Ramzan, Yasir Ramzan, Faisal Zulfiqar, Muhammad Ahsan and Ki Byung Lim
Plants 2020, 9(10), 1374; https://doi.org/10.3390/plants9101374 - 15 Oct 2020
Cited by 68 | Viewed by 9951
Abstract
Plants endure many abiotic stresses, such as temperature (heat or frost), drought, and salt. Such factors are primary and frequent stressors that reduce agriculture crop yields. Often alterations in nutrient management and constituents, along with variations in biosynthetic capacity, ultimately reduce or halt [...] Read more.
Plants endure many abiotic stresses, such as temperature (heat or frost), drought, and salt. Such factors are primary and frequent stressors that reduce agriculture crop yields. Often alterations in nutrient management and constituents, along with variations in biosynthetic capacity, ultimately reduce or halt plant growth. Genetically, stress is an environmental condition that interferes with complete genetic expression. A vast range of molecular genomic markers is available for the analysis of agricultural crops. These markers are classified into various groups based on how the markers are used: RAPD (Random amplified polymorphic DNA) markers serve to identify and screen hybrids based on salinity and drought stress tolerance, while simple sequence repeat (SSR) markers are excellent for the assessment of stress tolerance. Such markers also play an important role in the QTL (Quantitative trait loci) mapping of stress-related genes. Dehydrins for drought and saltol for salinity stresses are primitive genes which regulate responses to these conditions. Further, a focus on traits using single-gene single nucleotide polymorphisms (SNP) markers supports genetic mapping and the sequencing of stress-related traits in inbred lines. DNA markers facilitate marker-assisted breeding to enhance abiotic stress tolerance using advanced techniques and marker modification. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Genetic Engineering)
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