Advances and Applications of Genome Editing in Plants

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 31 July 2025 | Viewed by 1036

Special Issue Editors


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Guest Editor
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China
Interests: plant genome editing; crop breeding; rice

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Guest Editor
Institute of Biotechnology, College of Agriculture, Zhejiang University, Hangzhou 310058, China
Interests: genome editing; CRISPR/Cas; viral vector; transient delivery; plant virology; rhabdovirus; bunyavirus

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Guest Editor
Institute of Crop Sciences (ICS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China
Interests: genome editing; gene engineering; biotechnology; plant hormones; herbicide

Special Issue Information

Dear Colleagues,

The rapid development of genome editing technology has provided revolutionary molecular manipulation tools for plant breeding. A variety of gene editing tools with broad and precise editing ranges, such as different CRISPR systems, base editors, and prime editors, are emerging. However, currently, relatively complete gene editing systems have been established in only a few monocot crops, such as rice and maize, while related tools available for dicot plants like cotton and soybean are still very limited.

In this Special Issue, we invite researchers to submit original research articles and reviews that enhance our understanding of genome editing technology in plants.

We invite original research articles and reviews on topics including, but not limited to, the following:

  • The development of genome editing systems in plants, aiming to improve editing efficiency, enhance precision, facilitate multi-gene editing, and enable the precise insertion of large DNA fragments;
  • The performance of genome editing systems in various plants, particularly dicotyledonous plants and polyploid crops;
  • The development of efficient gene editing component delivery systems that are genotype-independent;
  • The creation of new germplasms using genome editing technology;
  • Innovations in breeding techniques utilizing genome editing technology.

Dr. Chun Wang
Prof. Dr. Zhenghe Li
Dr. Yubing He
Guest Editors

Manuscript Submission Information

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Keywords

  • genome editing
  • precise editing
  • genotype-independent transformation
  • crop breeding
  • germplasm innovation

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Published Papers (1 paper)

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Research

8 pages, 1005 KiB  
Communication
Establishment of a Genetic Transformation and Gene Editing Method by Floral Dipping in Descurainia sophia
by Tianjiao Jia, Hua Yang, Dingding Zhou, Sanzeng Zhao, Jianyong Wang, Tao Zhang, Mingkun Huang, Danyu Kong and Yi Liu
Plants 2024, 13(20), 2833; https://doi.org/10.3390/plants13202833 - 10 Oct 2024
Viewed by 791
Abstract
Descurainia sophia L. Webb ex Prantl is used in traditional medicine globally. However, the lack of an efficient and reliable genetic transformation system has seriously limited the investigation of gene function and further utilization of D. sophia. In this study, a highly [...] Read more.
Descurainia sophia L. Webb ex Prantl is used in traditional medicine globally. However, the lack of an efficient and reliable genetic transformation system has seriously limited the investigation of gene function and further utilization of D. sophia. In this study, a highly efficient, time-saving, and cost-effective Agrobacterium tumefaciens-mediated genetic transformation system has been developed in D. sophia. In this method, the transformation was accomplished by simply dipping developing D. sophia inflorescences for 45 s into an Agrobacterium suspension (OD600 = 0.6) containing 5% sucrose and 0.03% (v/v) Silwet L-77. Treated plants were allowed to set seeds which were then plated on a selective medium with hygromycin B (HygB) to screen transformants. Additionally, the CRISPR/Cas9 genomic editing system was validated by targeting phytoene desaturase (PDS) gene using this floral dip method, and mutant plants with the expected albino phenotype could be obtained in 2.5 months. This genetic transformation and targeted editing system will be a valuable tool for routine investigation of gene function and further exploitation in D. sophia. Full article
(This article belongs to the Special Issue Advances and Applications of Genome Editing in Plants)
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