Design of Biocatalytic System in Bioprocess Engineering

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Biological Processes and Systems".

Deadline for manuscript submissions: closed (31 May 2024) | Viewed by 3922

Special Issue Editors

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816 China
Interests: biocatalysis engineering; bioreaction engineering; bioseparation engineering; process intensification

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Guest Editor
School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China
Interests: biocatalysis; enzyme immobilization; cascade catalysis; asymmetric synthesis

Special Issue Information

Dear Colleagues,

Green bio-manufacturing will produce huge economic benefits for contributing to the development of the global bio-economy. The design of the biocatalytic system in bioprocess engineering is a critical initial step in green bio-manufacturing for the synthesis of chemical products, medical products, natural products, and flavor food. Feedstock, reaction medium, and biocatalysts are three main aspects that affect the reaction efficiency of the biocatalytic system. Therefore, its design needs to systematically the roles and interactions between these aspects. In order to synthesize high-value products, whole-cell catalysts, multi-enzyme catalysts, nano-biocatalysts, and the immobilization of biocatalysts have shown remarkable application value in the aqueous phase system, organic phase system, ionic liquid system, two-phase system, deep eutectic solvent system, and non-solvent system.

Dr. Hui Li
Dr. Yunting Liu
Guest Editors

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Keywords

  • novel biocatalysis platforms
  • whole-cell catalysis
  • multi-enzyme catalysis
  • nano-biocatalyst
  • immobilization of biocatalysts
  • non-aqueous biocatalysis
  • biocatalytic synthesis of natural products
  • biocatalytic synthesis of chemical products
  • biocatalytic synthesis of medical and health products
  • application of biocatalysis in food

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Published Papers (2 papers)

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Research

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8 pages, 1724 KiB  
Communication
Effects of His-Tag Length on the Soluble Expression and Selective Immobilization of D-Amino Acid Oxidase from Trigonopsis variabilis: A Preliminary Study
by Zhipeng Yan, Qinhe Zhu, Li Ma, Guihui Li, Erzheng Su, Jia Zeng, Yongzhong Chen, Er Meng and Senwen Deng
Processes 2023, 11(6), 1588; https://doi.org/10.3390/pr11061588 - 23 May 2023
Cited by 1 | Viewed by 2421
Abstract
His-tags are widely used for the purification of recombinant proteins. High-cost carriers functionalized with nickel ions are commonly required for the selective immobilization of His-tagged enzymes. In this study, His-tags of varying lengths were fused to the N-terminus of D-amino acid oxidase (DAO) [...] Read more.
His-tags are widely used for the purification of recombinant proteins. High-cost carriers functionalized with nickel ions are commonly required for the selective immobilization of His-tagged enzymes. In this study, His-tags of varying lengths were fused to the N-terminus of D-amino acid oxidase (DAO) from Trigonopsis variabilis. The attachment of a His6 tag significantly improved the solubility of the recombinant DAO expressed in Escherichia coli. By modulating the tag lengths, a better balance between cell growth and protein solubility was achieved, resulting in a higher volume activity (His3). Furthermore, the fusion of longer tags (His6 and His9) facilitated the rapid immobilization of DAOs onto a commercial epoxy carrier without metal bearing, resulting in more selective immobilization. In conclusion, the modulation of His-tag length was preliminarily demonstrated as a simple and cost-effective approach to achieve efficient expression, as well as fast and selective immobilization of DAO. Full article
(This article belongs to the Special Issue Design of Biocatalytic System in Bioprocess Engineering)
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Review

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16 pages, 576 KiB  
Review
Recent Progress in Non-Aqueous Biocatalysis of Immobilized Enzymes
by Jiayun Ma, Luyao Wang, Yan Chu, Yitong Wang, Kequan Chen and Hui Li
Processes 2024, 12(8), 1571; https://doi.org/10.3390/pr12081571 - 26 Jul 2024
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Abstract
Non-aqueous biocatalysis has attracted broad interest recently due to its differences from traditional aqueous catalysis and increased substrate solubility, which reduces feedback inhibition, improving enantiomer selectivity and completing synthesis reactions that cannot be performed in an aqueous solution. This approach shows remarkable application [...] Read more.
Non-aqueous biocatalysis has attracted broad interest recently due to its differences from traditional aqueous catalysis and increased substrate solubility, which reduces feedback inhibition, improving enantiomer selectivity and completing synthesis reactions that cannot be performed in an aqueous solution. This approach shows remarkable application value in producing natural products, chemical products, pharmaceutical intermediates, and foods. This study aims to provide a concise overview of the current state of non-aqueous biocatalysis and its sustainability, summarizing the mechanism of non-aqueous biocatalysis and recent progress using immobilization technology. It includes different non-aqueous systems, such as organic phase systems, two-phase systems, ionic liquid systems, deep eutectic solvent systems, and non-solvent systems. Finally, this manuscript illustrates the challenges of non-aqueous catalysis and the prospects of the future areas of non-aqueous catalysis research. Full article
(This article belongs to the Special Issue Design of Biocatalytic System in Bioprocess Engineering)
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