Optimization of three parameters: agitation rate (A; 100, 200 and 300 rpm), aeration rate (B; 0.5, 1.5 and 2.5 vvm) and aeration timing (C; 2, 4 and 6 h), for ethanol production from sweet sorghum juice under very high gravity (VHG, 290 g
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Optimization of three parameters: agitation rate (A; 100, 200 and 300 rpm), aeration rate (B; 0.5, 1.5 and 2.5 vvm) and aeration timing (C; 2, 4 and 6 h), for ethanol production from sweet sorghum juice under very high gravity (VHG, 290 g L
−1 of total sugar) conditions by
Saccharomyces cerevisiae NP 01 was attempted using an L
9 (3
4) orthogonal array design. The fermentation was carried out at 30 °C in a 2-L bioreactor and the initial yeast cell concentration was approximately 2 × 10
7 cells mL
−1. The results showed that the optimum condition for ethanol fermentation should be
A2B3C2 corresponding to agitation rate, 200 rpm; aeration rate, 2.5 vvm and aeration timing, 4 h. The verification experiments under the optimum condition clearly indicated that the aeration and agitation strategies improved ethanol production. The ethanol concentration (
P), productivity (
Qp) and ethanol yield (
Yp/s) were 132.82 ± 1.06 g L
−1, 2.55 ± 0.00 g L
−1h
−1 and 0.50 ± 0.00, respectively. Under the same condition without aeration (agitation rate at 200 rpm),
P and
Qp were only 118.02 ± 1.19 g L
−1 and 2.19 ± 0.04 g L
−1h
−1, respectively while
Yp/s was not different from that under the optimum condition.
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