Biomimetics, Volume 7, Issue 3 (September 2022) – 55 articles

The introduction of nanomaterials into polymethyl methacrylate (PMMA) resin has been effective for mechanically reinforcing PMMA for application in oral denture bases. However, these methods cannot simultaneously improve the mechanical and antibacterial properties, which limits widespread clinical application. Here, we self-assembled binary nanocomposites of boron nitride nanosheets (h-BNNs) and silver nanoparticles (AgNPs) as nanofillers and incorporated the nanofillers into PMMA. The aim of this study was to achieve antibacterial effects while significantly improving the mechanical properties of PMMA and provide a theoretical basis for further clinical application. We employed scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy (Raman), Ultraviolet visible spectrum (UV) and atomic force microscopy (AFM) to investigate the microscopic morphology and composition of PMMA containing nanocomposites with different mass fraction. In addition, the content of the h-BNNs/AgNPs was 1 wt%, and the compressive strength and flexural strength of pure PMMA were improved by 53.5% and 56.7%, respectively. When the concentration of the nanocomposite in the PMMA resin was 1.4 wt%, the antibacterial rate was 92.1%. Overall, synergistically reinforcing PMMA composite resin with a multi-dimensional nanocomposite structure provided a new perspective for expanding not only the application of resins in clinical settings but also the research and development of new composite resins. Full article

The main problems directly linked with the use of PMMA bone cements in orthopedic surgery are the improper mechanical bond between cement and bone and the absence of antimicrobial properties. Recently, more research has been devoted to new bone cement with antimicrobial properties using mainly antibiotics or other innovative materials with antimicrobial properties. In this paper, we developed modified PMMA bone cement with antimicrobial properties proposing some experimental antimicrobial agents consisting of silver nanoparticles incorporated in ceramic glass and hydroxyapatite impregnated with peppermint oil. The impact of the addition of antimicrobial agents on the structure, mechanical properties, and biocompatibility of new PMMA bone cements was quantified. It has been shown that the addition of antimicrobial agents improves the flexural strength of the traditional PMMA bone cement, while the yield strength values show a decrease, most likely because this agent acts as a discontinuity inside the material rather than as a reinforcing agent. In the case of all samples, the addition of antimicrobial agents had no significant influence on the thermal stability. The new PMMA bone cement showed good biocompatibility and the possibility of osteoblast proliferation (MTT test) along with a low level of cytotoxicity (LDH test). Full article

This study evaluates the effects of the availability of exogenous BMP-7 on osteoblastic cells’ differentiation on a nanotextured Ti surface obtained by chemical etching (Nano-Ti). The MC3T3-E1 and UMR-106 osteoblastic cell lines were cultured for 5 and 7 days, respectively, on a Nano-Ti surface and on a control surface (Control-Ti) in an osteogenic medium supplemented with either 40 or 200 ng/mL recombinant mouse (rm) BMP-7. The results showed that MC3T3-E1 cells exhibited distinct responsiveness when exposed to each of the two rmBMP-7 concentrations, irrespective of the surface. Even with 40 ng/mL rmBMP-7, important osteogenic effects were noticed for Control-Ti in terms of cell proliferation potential; Runx2, Osx, Alp, Bsp, Opn, and Smad1 mRNA expression; and in situ ALP activity. For Nano-Ti, the effects were limited to higher Alp, Bsp, and Opn mRNA expression and in situ ALP activity. On both surfaces, the osteogenic potential of UMR-106 cultures remained unaltered with 40 ng/mL rmBMP-7, but it was significantly reduced when the cultures were exposed to the 200 ng/mL concentration. The availability of rmBMP-7 to pre-osteoblastic cells at the concentrations used alters the expression profile of osteoblast markers, indicative of the acquisition of a more advanced stage of osteoblastic differentiation. This occurs less pronouncedly on the nanotextured Ti and without reflecting in higher mineralized matrix production by differentiated osteoblasts on both surfaces. Full article

The lightweight property of helical composite spring (HCS) applied in the transportation field has attracted more and more attention recently. However, it is difficult to maintain stiffness and fatigue resistance at the same time. Herein, inspired by collagen fibers in bone, a bionic basalt fiber/epoxy resin helical composite spring is manufactured. The collagen fibers consist of nanoscale hydroxyapatite (increases stiffness) and collagen molecules composed of helical amino acid chains (can increase fatigue resistance). Such a helical structure of intercalated crystals ensures that bone has good resistance to fracture. Specifically, we first investigated the effect of adding different contents of NS to basalt fibers on the stiffness and fatigue properties of an HCS. The results show that the optimal NS content of 0.4 wt% resulted in 52.1% and 43.5% higher stiffness and fatigue properties of an HCS than those without NS, respectively. Then, two braided fiber bundles (TS-BFB) and four braided fiber bundles (FS-BFB) were designed based on the helical structure of amino acid chains, and the compression tests revealed that the maximum load resistance of TS-BFB and FS-BFB was increased by 29.2% and 44%, respectively, compared with the conventional single fiber bundle (U-BFB). The superior mechanical performance of TS-BFB and FS-BFB is attributed to the more adequate bonding of 0.4 wt% NS to the epoxy resin and the multi-fiber bundles that increase the transverse fiber content of the spring. The findings in this work introduce the bionic collagen fiber structure into the design for an HCS and provide a new idea to improve the spring performance. Full article

Controlled, reversible attachment is widely spread throughout the animal kingdom: from ticks to tree frogs, whose weights span from 2 mg to 200 g, and from geckos to mosquitoes, who stick under vastly different situations, such as quickly climbing trees and stealthily landing on human hosts. A fascinating and complex interplay of adhesive and frictional forces forms the foundation of attachment of these highly diverse systems to various substrates. In this review, we present an overview of the techniques used to quantify the adhesion and friction of terrestrial animals, with the aim of informing future studies on the fundamentals of bioadhesion, and motivating the development and adoption of new or alternative measurement techniques. We classify existing methods with respect to the forces they measure, including magnitude and source, i.e., generated by the whole body, single limbs, or by sub-structures. Additionally, we compare their versatility, specifically what parameters can be measured, controlled, and varied. This approach reveals critical trade-offs of bioadhesion measurement techniques. Beyond stimulating future studies on evolutionary and physicochemical aspects of bioadhesion, understanding the fundamentals of biological attachment is key to the development of biomimetic technologies, from soft robotic grippers to gentle surgical tools. Full article

This study aimed to evaluate the effects of magnesium oxide (MgO) nanoparticle (NP) incorporation on shear bond strength (SBS) and antibacterial property of orthodontic composites. A total of 100 mounted premolar teeth were randomly divided into five groups. In group 1 (control), the brackets were bonded to the teeth using the GC Ortho Connect orthodontic composite, while the brackets of groups 2 to 5 were bonded by the GC Ortho Connect orthodontic composite that contained 0.5%, 1%, 2%, and 4% weight percentages (w/w) of MgO NPs, respectively and then the SBS was measured. In the following, we evaluated the antibacterial properties of the MgO NP-containing composite on Streptococcus mutans (S. mutans) bacteria by the direct contact test method. According to results, there were no significant changes in the SBS as the MgO NP concentration was increased up to 1%, while the SBSs of the 2% and 4% MgO NPs were decreased when compared to the other three groups. The outcomes of the direct contact test indicated the case of 1% as being the minimum ratio of MgO NPs, which almost caused the entire annihilation of the S. mutans bacteria. In conclusion, the orthodontic composite containing 1% MgO NPs can display a significant antibacterial effect against S. mutans bacteria without inducing any negative effect on the SBS. Full article

In this paper, a superhydrophobic biomimetic composite coating was fabricated on brass by electrochemical etching, brushing PDMS adhesive layer, and depositing carbon soot particles. Due to the microstructure and the optimized ratio of PDMS, the contact angle of the superhydrophobic coating is up to 164° and the sliding angle is only 5°. The results of optical microscopy and morphometric laser confocal microscopy show that the prepared coating surface has a rough hierarchical structure. A high-speed digital camera recorded the droplet bouncing process on the surface of the superhydrophobic coating. The self-cleaning property of the coatings was evaluated by applying chalk dust particles as simulated solid contaminants and different kinds of liquids (including grape juice, beer, cola, and blue ink) as liquid contaminants. The coating remained superhydrophobic after physical and chemical damage tests. This work presents a strategy for fabricating superhydrophobic biomimetic composite coatings with significant self-cleaning properties, durability, and shows great potential for practical engineering applications. Full article

Tissue engineering to develop alternatives for the maintenance, restoration, or enhancement of injured tissues and organs is gaining more and more attention. In tissue engineering, the scaffold used is one of the most critical elements. Its characteristics are expected to mimic the native extracellular matrix and its unique topographical structures. Recently, the topographies of scaffolds have received increasing attention, not least because different topographies, such as aligned and random, have different repair effects on various tissues. In this review, we have focused on various technologies (electrospinning, directional freeze-drying, magnetic freeze-casting, etching, and 3-D printing) to fabricate scaffolds with different topographic orientations, as well as discussed the physicochemical (mechanical properties, porosity, hydrophilicity, and degradation) and biological properties (morphology, distribution, adhesion, proliferation, and migration) of different topographies. Subsequently, we have compiled the effect of scaffold orientation on the regeneration of vessels, skin, neural tissue, bone, articular cartilage, ligaments, tendons, cardiac tissue, corneas, skeletal muscle, and smooth muscle. The compiled information in this review will facilitate the future development of optimal topographical scaffolds for the regeneration of certain tissues. In the majority of tissues, aligned scaffolds are more suitable than random scaffolds for tissue repair and regeneration. The underlying mechanism explaining the various effects of aligned and random orientation might be the differences in “contact guidance”, which stimulate certain biological responses in cells. Full article

Diagnostic ultrasound or sonography is an image that can provide valuable information for diagnosing and treating a variety of diseases and conditions. The aim of this research study is to examine the performance and accuracy of the ultrasonic imaging system for the guarantee of diagnosis quality assurance, and to adjust the penetration settings to minimize the time of repeat scans and maintenance duration during research experiments. Measurements in this experiment included the resolution (axial and lateral) and focal zones. Moreover, the evaluation was done by completing all the measurements at different depths on a multipurpose phantom model 539. The phantom was bought from the market and was not fabricated by the author. The measurements were achieved by applying two different transducers: curved and linear (flat). The ultrasound images were obtained and tested by using calipers (electronic), and the estimations and observations were read by using all the taken measurements and images. As a result, because the phantom depths were different, the penetration settings were different too, indicating that the depth impacted the penetrations of the created ultrasound image. Moreover, after the comparison of the recorded measurements and results, it was found that all measurements were within the accepted (standard) value and that the true value was specified by the production of the phantom. Full article

Fungi-based materials (myco-materials) have been celebrated and experimented with for their architectural and structural potential for over a decade. This paper describes research applied to assembly strategies for growing large building units and assembling them into efficiently formed wall prototypes. A major concern in the development of these two fabrication strategies is to design re-usable formwork systems. La Parete Fungina demonstrates two undulating wall units standing side-by-side, each composed of seventeen myco-welded slabs. L’Orso Fungino revisits the in situ monolithic fabric forming of units that are repeated, stacked, and post-tensioned. Although the design and research presented in this paper focuses on overcoming the challenges of growing large-scale building components, this work also touches on issues of accessibility and technology, economic and logistical systems needed for building-scale applications, and material ethics of energy and waste associated with emerging biomaterial production. Full article

An alula is a small structure of feathers that prevents birds from stalling. In this study, the aerodynamic effect of an alula-like vortex generator (alula-VG) on a revolving wing was investigated using the PIV technique in a water tank. The alula-VG was mounted on a rectangular wing model at two spanwise positions. The wing model with a revolving motion was installed at different angles of attack, which included pre-stall and post-stall conditions. The velocity fields around the wing model with/without an alula-VG were measured and analyzed, including the vorticity contour, the circulation of vortex structures, and the corresponding sectional lift coefficient, which are used to explain the aerodynamic effect induced by an alula-VG. The lift-off and bursting of the leading-edge vortex (LEV) affect the magnitude of the chordwise circulation and the section lift coefficient. The results show that compared to an alula-VG mounted fixed wing model, the flow interactions among the alula-VG induced spanwise flow, the inertial force caused by the revolving motion, and the wing-tip vortex play important roles in the vortex bursting and the resultant aerodynamic performance. The effect of an alula-VG on a revolving wing depends on its spanwise position and the angle of attack of a wing model, which need to be properly matched. Full article

European law now requires AI to be explainable in the context of adverse decisions affecting the European Union (EU) citizens. At the same time, we expect increasing instances of AI failure as it operates on imperfect data. This paper puts forward a neurally inspired theoretical framework called “decision stacks” that can provide a way forward in research to develop Explainable Artificial Intelligence (X-AI). By leveraging findings from the finest memory systems in biological brains, the decision stack framework operationalizes the definition of explainability. It then proposes a test that can potentially reveal how a given AI decision was made. Full article

Nanoparticles (NPs) are usually treated as multifunctional agents combining several therapeutical applications, like imaging and targeting delivery. However, clinical translation is still largely hindered by several factors, and the rapidly formed protein corona on the surface of NPs is one of them. The formation of protein corona is complicated and irreversible in the biological environment, and protein corona will redefine the “biological identity” of NPs, which will alter the following biological events and therapeutic efficacy. Current understanding of protein corona is still limited and incomplete, and in many cases, protein corona has adverse impacts on nanomedicine, for instance, losing targeting ability, activating the immune response, and rapid clearance. Due to the considerable role of protein corona in NPs’ biological fate, harnessing protein corona to achieve some therapeutic effects through various methods like biomimetic approaches is now treated as a promising way to meet the current challenges in nanomedicine such as poor pharmacokinetic properties, off-target effect, and immunogenicity. This review will first introduce the current understanding of protein corona and summarize the investigation process and technologies. Second, the strategies of harnessing protein corona with biomimetic approaches for nanomedicine design are reviewed. Finally, we discuss the challenges and future outlooks of biomimetic approaches to tune protein corona in nanomedicine. Full article

Adhesion robots have broad application prospects in the field of spacecraft inspection, repair, and maintenance, but the stable adhesion and climbing on the flexible surface covering the spacecraft has not been achieved. The flexible surface is easily deformed when subjected to external force, which makes it difficult to ensure a sufficient contact area and then detach from it. To achieve stable attachment and easy detachment on the flexible surface under microgravity, an adhesion model is established based on the applied adhesive material, and the relationship between peeling force and the rigidity of the base material, peeling angle, and working surface stiffness is obtained. Combined with the characteristics of variable stiffness structure, the adhesion and detachment force of the foot is asymmetric. Inspired by the adhesion-detachment mechanism of the foot of the gecko, an active adhesion-detachment control compliant mechanism is designed to achieve the stable attachment and safe detachment of the foot on the flexible surface and to adapt to surfaces with different rigidity. The experimental results indicate that a maximum normal adhesion force of 7.66 N can be generated when fully extended, and the safe detachment is achieved without external force on a flexible surface. Finally, an air floating platform is used to build a microgravity environment, and the crawling experiment of a gecko-inspired robot on a flexible surface under microgravity is completed. The experimental results show that the gecko-inspired foot with variable stiffness can satisfy the requirements of stable crawling on flexible surfaces. Full article

The recently emerging multi-portfolio selection problem lacks a proper framework to ensure that client privacy and database secrecy remain intact. Since privacy is of major concern these days, in this paper, we propose a variant of Beetle Antennae Search (BAS) known as Distributed Beetle Antennae Search (DBAS) to optimize multi-portfolio selection problems without violating the privacy of individual portfolios. DBAS is a swarm-based optimization algorithm that solely shares the gradients of portfolios among the swarm without sharing private data or portfolio stock information. DBAS is a hybrid framework, and it inherits the swarm-like nature of the Particle Swarm Optimization (PSO) algorithm with the BAS updating criteria. It ensures a robust and fast optimization of the multi-portfolio selection problem whilst keeping the privacy and secrecy of each portfolio intact. Since multi-portfolio selection problems are a recent direction for the field, no work has been done concerning the privacy of the database nor the privacy of stock information of individual portfolios. To test the robustness of DBAS, simulations were conducted consisting of four categories of multi-portfolio problems, where in each category, three portfolios were selected. To achieve this, 200 days worth of real-world stock data were utilized from 25 NASDAQ stock companies. The simulation results prove that DBAS not only ensures portfolio privacy but is also efficient and robust in selecting optimal portfolios. Full article

This paper aims to understand the aerodynamic performance of a bio-inspired flapping-wing model using the dwarf Kingfisher wing as the bionic reference. The paper demonstrates the numerical investigation of the Kingfisher-inspired flapping-wing followed by experimental validation to comprehend the results fully and examine the aerodynamic characteristics at a flight velocity of 4.4 m/s, with wingbeat frequencies of 11 Hz, 16 Hz, and 21 Hz, at various angles of rotation ranging from 0° to 20° for each stroke cycle. The motivation to study the performance at low speed is based on lift generation as a challenge at low speed as per quasi-steady theory. The temporal evolution of the mean force coefficients has been plotted for various angles of rotation. The results show amplification of the maximum value for the cycle average lift and drag coefficient as the rotation angle increases. The history of vertical force and the flow patterns around the wing is captured in a full cycle with asymmetric lift development in a single stroke cycle. It is observed from the results that the downstroke generates more lift force in magnitude compared to the upstroke. In addition to the rotation angle, lift asymmetry is also affected by wing–wake interaction. Experimental results reveal that there is a stable leading-edge vortex developed in the downstroke, which sheds during the upstroke. An optimum lift and thrust flapping flight can be achieved, with a lift coefficient of 3.45 at 12°. The experimental and parametric study results also reveal the importance of passive rotation in wings for aerodynamic performance and wing flexibility as an important factor for lift generation. Full article

The lack of drinkable water is one of the most significant risks for the future of the humanity. Estimates show that in the near future, this risk will become the origin of massive migrations leading to humanitarian disaster. As consequence, the development of solutions to provide water is becoming ever more critical, and a significant effort is devoted to identifying new sources of water. Among the developed strategies, fog harvesting, which takes advantage of atmospheric water to provide potable water, is a solution of interest due to its potential in sustainable development. Unfortunately, this approach suffers from low yield. In the present work, we take inspiration from living species to design and elaborate surfaces with high potential for water harvesting applications. This work takes advantage of 3D-printing and post-printing functionalization to elaborate a strategy that allows modelling, printing, and functionalization of surfaces to yield parahydrophobic behavior. The roughness and surface morphology of the prepared surfaces were investigated. These characteristics were then related to the observed wettability and potential of the functionalized interfaces for water harvesting applications. This work highlights significant variations in surface wettability via surface modification; strong hydrophobic behavior was observed via modification with linear carboxylic acids particularly for surfaces bearing vertical blades (plate with vertical blades and grid with vertical blades). Full article

Calcium phosphate cement (CPC) is similar to bone in composition and has plasticity, while mesoporous bioactive glass (MBG) has the advantage of releasing Si, which can promote osteogenic properties and drug loading capacity. A sol–gel-prepared MBG micro-powder (mMBG) and further impregnated antibiotic gentamicin sulfate (Genta@mMBG: 2, 3, and 4 mg/mL) antibiotic were added to CPC at different weight ratios (5, 10, and 15 wt.%) to study CPC’s potential clinical applications. Different ratios of mMBG/CPC composite bone cement showed good injectability and disintegration resistance, but with increasing mMBG addition, the working/setting time and compressive strength decreased. The maximum additive amount was 10 wt.% mMBG due to the working time of ~5 min, the setting time of ~10 min, and the compressive strength of ~51 MPa, indicating that it was more suitable for clinical surgical applications than the other groups. The 2Genta@mMBG group loaded with 2 mg/mL gentamicin had good antibacterial activity, and the 10 wt.% 2Genta@mMBG/CPC composite bone cement still had good antibacterial activity but reduced the initial release of Genta. 2Genta@mMBG was found to have slight cytotoxicity, so 2Genta@mMBG was composited into CPC to improve the biocompatibility and to endow CPC with more advantages for clinical application. Full article

The toughening mechanism of the nacre was widely investigated in recent decades, which presents a great prospect for designing high performance composite materials and engineering structures with bioinspired structures. To further elucidate which structural parameters and which kinds of morphology of the nacre-inspired structure are the best for improving tensile strength without sacrificing too much toughness is extremely significant for composite materials and engineering structures. The “brick-and-mortar” structure is a classical nacre-inspired bionic structure. Three characteristic structural parameters, including the aspect ratio ρ of the brick length and width, the thickness ratio β between the thickness of brick and mortar, and the spacing ratio τ between the width of brick and mortar, were used as variables to study their effect on tensile strength and toughness. It was found that ρ was the most prominent factor in determining the strength and toughness, and τ could improve the strength and toughness almost simultaneously. Racked and wedged morphology of the structural unit were established based on the structural parameters of the regular staggered unit, and were used to compare tensile behavior. It was found that the model with the wedged unit possessed the highest strength and toughness, and could absorb more strain energy during fracture crack growing. The crack propagation path further illustrated that the crack resisting ability of the wedged unit was the best. Our simulation results presented the connection between three characteristic structural parameters with the strength and toughness, and proved that the wedged staggered unit was the best in improving the strength and toughness. Full article

The terminal toes of adhesive animals are characterized by rigid-flexible coupling, and their structure–function relationship is an urgent problem to be solved in understanding bioinspired adhesive systems and the design of biomimetic adhesive units. In this paper, inspired by the rigid-flexible coupling adhesive system of the gecko toe, a rigid-flexible coupling adhesive unit was designed, the interface strength of the adhesives under different preloads was tested, and the model and analysis method of the compression and peeling process of the rigid-flexible coupling adhesive unit was established. Meanwhile, combined with the experimental test, the effect of the coupling mechanism of the rigid-flexible structure on the interfacial stress and the final peeling force during the compression and peeling process of the adhesive unit was studied. The research found that the length of the adhesive unit L has no apparent effect on the normal peel force of the system within a specific range, and the normal peeling force increases linearly with the increase in the compression force P; while the influence of the inclination angle θ₀ of the adhesive unit and the thickness of the rigid backing layer h_b on the final normal peeling force of the system presents nonlinear characteristics, when the inclination angle θ₀ of the adhesive unit is 5°, and the thickness of the rigid backing layer h_b is 0.2 mm or 0.3 mm, the normal peel force and the ratio of adhesion force to preload the system reaches its maximum value. Compared with the flexible adhesive unit, the compressed zone formed by the rigid-flexible coupling adhesive unit during the same compression process increased by 6.7 times, while under the same peeling force, the peel zone increased by 8 times, and the maximum normal tensile stress at the peeling end decreased by 20 times. The rigid-flexible coupling mechanics improves the uniformity of the contact stress during the compression and peeling process. The research results provide guidelines for the design of the rigid-flexible coupling adhesive unit, further providing the end effector of the bionic wall-climbing robot with a rigid-flexible coupled bionic design. Full article

Velopharyngeal insufficiency may occur as a result of an anatomical or structural defect and may be present in patients with cleft lip and palate. The treatment options presented in the literature are varied, covering invasive and non-invasive methods. However, although these approaches have been employed and their outcomes reviewed, no conclusions have been made about which approach is the gold-standard. This umbrella review aimed to synthesize the current literature regarding velopharyngeal insufficiency treatments in cleft lip and palate patients, evaluating their effectiveness based on systematic reviews. A standardized search was carried out in several electronic databases, namely PubMed via Medline, Web of Science, Cochrane Library, and Embase. The quality of the included studies was evaluated using AMSTAR2 and degree of overlap was analyzed using Corrected Covered Area. Thirteen articles were included in the qualitative review, with only 1 in the non-invasive method category, and 12 in the invasive method category. All reviewed articles were judged to be of low quality. In symptomatic patients, treatment did not solely comprise speech therapy, as surgical intervention was often necessary. Although there was no surgical technique considered to be the gold standard for the correction of velopharyngeal insufficiency, the Furlow Z-plasty technique and minimal incision palatopharyngoplasty were the best among reported techniques. Full article

An aging global population is accelerating the need for better, longer-lasting orthopaedic and dental implants. Additive manufacturing can provide patient-specific, titanium-alloy-based implants with tailored, three-dimensional, bone-like architecture. Studies using two-dimensional substrates have demonstrated that osteoblastic differentiation of bone marrow stromal cells (MSCs) is enhanced on surfaces possessing hierarchical macro/micro/nano-scale roughness that mimics the topography of osteoclast resorption pits on the bone surface. Conventional machined implants with these surfaces exhibit successful osseointegration, but the complex architectures produced by 3D printing make consistent nanoscale surface texturing difficult to achieve, and current line-of-sight methods used to roughen titanium alloy surfaces cannot reach all internal surfaces. Here, we demonstrate a new, non-line-of-sight, gas/solid-reaction-based process capable of generating well-controlled nanotopographies on all open (gas-exposed) surfaces of titanium alloy implants. Dense 3D-printed titanium-aluminum-vanadium (TiAl6V4) substrates were used to evaluate the evolution of surface nanostructure for development of this process. Substrates were either polished to be smooth (for easier evaluation of surface nanostructure evolution) or grit-blasted and acid-etched to present a microrough biomimetic topography. An ultrathin (90 ± 16 nm) conformal, titania-based surface layer was first formed by thermal oxidation (600 °C, 6 h, air). A calciothermic reduction (CaR) reaction (700 °C, 1 h) was then used to convert the surface titania (TiO₂) into thin layers of calcia (CaO, 77 ± 16 nm) and titanium (Ti, 51 ± 20 nm). Selective dissolution of the CaO layer (3 M acetic acid, 40 min) then yielded a thin nanoporous/nanorough Ti-based surface layer. The changes in surface nanostructure/chemistry after each step were confirmed by scanning and transmission electron microscopies with energy-dispersive X-ray analysis, X-ray diffraction, selected area electron diffraction, atomic force microscopy, and mass change analyses. In vitro studies indicated that human MSCs on CaR-modified microrough surfaces exhibited increased protein expression associated with osteoblast differentiation and promoted osteogenesis compared to unmodified microrough surfaces (increases of 387% in osteopontin, 210% in osteocalcin, 282% in bone morphogenic protein 2, 150% in bone morphogenic protein 4, 265% in osteoprotegerin, and 191% in vascular endothelial growth factor). This work suggests that this CaR-based technique can provide biomimetic topography on all biologically facing surfaces of complex, porous, additively manufactured TiAl6V4 implants. Full article

Morphology, thermal properties and the non-isothermal melt crystallization kinetics of biodegradable poly(ε-caprolactone) (PCL)/reduced graphene oxide (rGO) scaffolds are studied with differential scanning calorimetry (DSC) at various cooling rates (5, 10, 15 and 20 °C/min). Thermally induced phase separation was used to manufacture the scaffolds (TIPS). The micrographs show a more homogeneous and defined morphology with larger pores and thicker pore walls. The melting temperature (Tm), melting enthalpy (ΔHm), crystallization enthalpy (ΔHc) and degree of crystallinity (Xc) increased with the addition of rGO, suggesting larger and more perfect crystalline structures. The degree of crystallinity increased with the presence of rGO. The crystallization peak shifted to higher temperatures as the rGO concentration increased independently of the cooling rates. The peak shifted to lower temperatures as the cooling rate increased with the same rGO composition. The values of t_1/2 (time needed to reach 50% crystallization) were lower for scaffolds with rGO. The values of the crystallization rate coefficient were higher when the porous support contained rGO, which indicates that their crystallization systems are faster. The activation energy obtained with the Kissinger method decreased with the presence of rGO. The results indicate that reduced graphene oxide acts as a nucleating agent in the non-isothermal melt crystallization process. The addition of small quantities of rGO changes their thermal properties with which they can be modified for application in the field of tissue engineering. Full article

As one of few animals with the capability to execute agile yawing maneuvers, it is quite desirable to take inspiration from hummingbird flight aerodynamics. To understand the wing and body kinematics and associated aerodynamics of a hummingbird performing a free yawing maneuver, a crucial step in mimicking the biological motion in robotic systems, we paired accurate digital reconstruction techniques with high-fidelity computational fluid dynamics (CFD) simulations. Results of the body and wing kinematics reveal that to achieve the pure yaw maneuver, the hummingbird utilizes very little body pitching, rolling, vertical, or horizontal motion. Wing angle of incidence, stroke, and twist angles are found to be higher for the inner wing (IW) than the outer wing (OW). Unsteady aerodynamic calculations reveal that drag-based asymmetric force generation during the downstroke (DS) and upstroke (US) serves to control the speed of the turn, a characteristic that allows for great maneuvering precision. A dual-loop vortex formation during each half-stroke is found to contribute to asymmetric drag production. Wake analysis revealed that asymmetric wing kinematics led to leading-edge vortex strength differences of around 59% between the IW and OW. Finally, analysis of the role of wing flexibility revealed that flexibility is essential for generating the large torque necessary for completing the turn as well as producing sufficient lift for weight support. Full article

This paper reports on a transformable sheet type robot that can be thrown from the air. Since sheet type robots can change their own shape and perform tasks according to the situation, they are expected to play an active role in situations with many restrictions, such as disaster-stricken areas. However, since most sheet type robots jump or crawl on the ground, the only way to deliver them to the site of a disaster is to transport them by vehicle or transporter. This research aims to develop a device that can be dispersed from the sky and perform activities on the ground after landing. Full article

In nature, live fish has various deformable fins which are capable to promote the swimming speed, efficiency, stability, and thrust generation. However, this feature is rarely possessed by current man-made biomimetic robotic fishes. In this paper, a novel deformable caudal fin platform is proposed to improve thrust generation of biomimetic robotic fish. First, the design of the deformable caudal fin is given, which includes a servo motor, a gear-based transmission mechanism, fin bones, and silica membrane. Second, an improved Central Pattern Generator (CPG) model was developed to coordinately control the flapping of the tail and the deformation of the caudal fin. More specifically, three deformation patterns, i.e., conventional nondeformable mode, sinusoidal-based mode, instant mode, of the caudal fin are investigated. Third, extensive experiments are conducted to explore the effects of deformation of the caudal fin on the thrust generation of the biomimetic robotic fish. It was found that the instant mode of the caudal fin has the largest thrust, which sees a 27.5% improvement compared to the conventional nondeformable mode, followed by the sinusoidal-based mode, which also sees an 18.2% improvement. This work provides a novel way to design and control the deformation of the caudal fin, which sheds light on the development of high-performance biomimetic robotic fish. Full article

Bone is a complex biologic tissue, which is extremely relevant for various physiological functions, in addition to movement, organ protection, and weight bearing. The repair of critical size bone defects is a still unmet clinical need, and over the past decades, material scientists have been expending efforts to find effective technological solutions, based on the use of scaffolds. In this context, biomimetics which is intended as the ability of a scaffold to reproduce compositional and structural features of the host tissues, is increasingly considered as a guide for this purpose. However, the achievement of implants that mimic the very complex bone composition, multi-scale structure, and mechanics is still an open challenge. Indeed, despite the fact that calcium phosphates are widely recognized as elective biomaterials to fabricate regenerative bone scaffolds, their processing into 3D devices with suitable cell-instructing features is still prevented by insurmountable drawbacks. With respect to biomaterials science, new approaches maybe conceived to gain ground and promise for a substantial leap forward in this field. The present review provides an overview of physicochemical and structural features of bone tissue that are responsible for its biologic behavior. Moreover, relevant and recent technological approaches, also inspired by natural processes and structures, are described, which can be considered as a leverage for future development of next generation bioactive medical devices. Full article

In this report, we demonstrated the formation of a biomimetic mineralizing layer obtained on the surface of dental enamel (biotemplate) using bioinspired nanocrystalline carbonate-substituted calcium hydroxyapatite (ncHAp), whose physical and chemical properties are closest to the natural apatite dental matrix, together with a complex of polyfunctional organic and polar amino acids. Using a set of structural, spectroscopy, and advanced microscopy techniques, we confirmed the formation of a nanosized ncHAp-based mineralized layer, as well as studying its chemical, substructural, and morphological features by means of various methods for the pretreatment of dental enamel. The pretreatment of a biotemplate in an alkaline solution of Ca(OH)₂ and an amino acid booster, together with the executed subsequent mineralization with ncHAp, led to the formation of a mineralized layer with homogeneous micromorphology and the preferential orientation of the ncHAp nanocrystals. It was shown that the homogeneous crystallization of hydroxyapatite on the biotemplate surface and binding of individual nanocrystals and agglomerates into a single complex by an amino acid booster resulted in an increase (~15%) in the nanohardness value in the enamel rods area, compared to that of healthy natural enamel. Obtaining a similar hierarchy and cleavage characteristics as natural enamel in the mineralized layer, taking into account the micromorphological features of dental tissue, is an urgent problem for future research. Full article

In this study, two boronic acid BODIPYs are obtained through a microwave-assisted Knoevenagel reaction. The aim is to use them for the first time as dyes in a photosensitized solar cell (DSSC) to mimic chlorophyll photosynthesis, harvesting solar light and converting it into electricity. The microwave-assisted Knoevenagel reaction is a straightforward approach to extending the molecular conjugation of the dye and is applied for the first time to synthesize BODIPY’s boronic acid derivatives. These derivatives have proved to be very useful for covalent deposition on titania. This work studies the photo-physical and electrochemical properties. Moreover, the photovoltaic performances of these two new dyes as sensitizers for DSSC are discussed. Experimental data show that both dyes exhibit photosensitizing activities in acetonitrile and water. In particular, in all the experiments, distyryl BODIPY was more efficient than styryl BODIPY. In this study, demonstrating the use of a natural component as a water-based electrolyte for boronic BODIPY sensitizers, we open new possibilities for the development of water-based solar cells. Full article

Encapsulation comprises a promising potential for the targeted delivery of entrapped sensitive agents into the food system. A unique combination of cellulose/chitosan (Cl-Ch)-based hybrid wall material was employed to encapsulate L. plantarum by emulsion technique. The developed beads were further subjected to morphological and in vitro studies. The viability of free and encapsulated probiotics was also evaluated in kefir during storage. The developed beads presented porous spherical structures with a rough surface. A 1.58 ± 0.02 log CFU/mL, 1.26 ± 0.01 log CFU/mL, and 1.82 ± 0.01 log CFU/mL reduction were noticed for Cl-Ch hybrid cells under simulated gastro-intestinal and thermal conditions, respectively. The encapsulated cells were found to be acidic and thermally resistant compared to the free cells. Similarly, encapsulated probiotics showed better viability in kefir at the end of the storage period compared to free cells. In short, the newly developed Cl-Ch hybrid-based encapsulation has a promising potential for the targeted delivery of probiotics, as career agents, in gastric transit, and in foods. Full article