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Foods
Special Issues
Food Safety Detection Analysis and Sensors
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Food Safety Detection Analysis and Sensors

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: 30 August 2025 | Viewed by 2966

Special Issue Editor

Dr. Xiangmei Li

E-Mail Website
Guest Editor
College of Food Science, South China Agricultural University, Guangzhou 510642, China
Interests: food analysis; immunoassay; antibody engineering; hapten design; biosensor; food safety; nanomaterials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Food safety is an important interdisciplinary field that studies the processes of food processing, storage, and sales to ensure food hygiene and safety, reduce disease risks, and prevent food poisoning. Food safety detection is the last line of defense to ensure food safety. Food safety is influenced by various factors, including environmental factors, chemical factors, and biological factors. It is very important to establish fast food safety detection sensors to address these influencing factors. Among them are sensors based on biological or chemical core components such as antibodies, nucleic acid aptamers, and molecularly imprinted polymers, combined with various new nanomaterials such as MOFs, MXenes, aggregation-induced luminescent materials, etc. Establishing sensitive and rapid sensors for various chemical or biological pollutants such as pesticides, veterinary drugs, mycotoxins, illegal additives, harmful heavy metals, foodborne pathogenic microorganisms, etc., integrating them with portable intelligent quantitative detection equipment, and applying them to the rapid determination of food safety are currently hot topics of research.

Dr. Xiangmei Li
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • food safety
  • rapid detection
  • sensor
  • immunoassay
  • nanomaterials
  • veterinary medicine
  • pesticide
  • mycotoxins
  • heavy metal
  • foodborne microorganisms
  • illegal additives

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Published Papers (3 papers)

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Research

14 pages, 4417 KiB  
Article
Development of a Dual-Readout Multicolor Immunoassay for the Rapid Analysis of Isocarbophos in Vegetable and Fruit Samples
by Zijian Chen, Wei-Xuan Huang, Hongwu Wang, Meiling Zhang, Kai Chen and Hao Deng
Foods 2024, 13(24), 4057; https://doi.org/10.3390/foods13244057 - 16 Dec 2024
Viewed by 638
Abstract
Multicolor immunoassay is a powerful tool for rapid analysis without the use of bulky instruments owing to various color conversions, which is suitable for on-site visual analysis for pesticides. Herein, this study developed a multicolor immunoassay for the rapid detection of isocarbophos. After [...] Read more.
Multicolor immunoassay is a powerful tool for rapid analysis without the use of bulky instruments owing to various color conversions, which is suitable for on-site visual analysis for pesticides. Herein, this study developed a multicolor immunoassay for the rapid detection of isocarbophos. After competitive immunoassay, the secondary antibody (GAM-ALP) catalyzed ascorbyl-2-phosphate (AAP) into ascorbic acid (AA). The AA can reduce K3[Fe(CN)6] into K4[Fe(CN)6]. The latter can react with Fe3+ to form Prussian blue; otherwise, the orange AAP-Fe3+ complex was generated. Therefore, the multicolor immunoassay achieved a color conversion of orange–green–blue in response to isocarbophos, allowing for rapid semiquantitative analysis by the naked eye. After parameter optimization, the multicolor immunoassay was developed depending on the ratiometric absorbance between the Prussian blue and AAP-Fe3+ complex. Moreover, a smartphone was used to measure the RGB value of the color conversion for the development of portable visual, quantitative analysis. Both the absorbance-based and RGB-based multicolor immunoassays showed good accuracy and practicability in the recovery test. This study provided a common approach for the development of dual-readout multicolor immunoassay, which can be used for on-site rapid screening by quantitative or visual semiquantitative analysis. Full article
(This article belongs to the Special Issue Food Safety Detection Analysis and Sensors)
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14 pages, 3475 KiB  
Article
Validation of a Novel Strategy for Fluorescence Quenching for a Self-Quenching Fluorogenic Probe and Its Application for Visual Loop-Mediated Isothermal Amplification Detection During Food Safety Analysis
by Sisi Huang, Shihui Wang, Tianlong Wang, Hongwei Song, Yan Guo, Xiong Xiong and Libin Wang
Foods 2024, 13(23), 3816; https://doi.org/10.3390/foods13233816 - 26 Nov 2024
Viewed by 961
Abstract
The self-quenching fluorogenic probe facilitates precise identification of LAMP (loop-mediated isothermal amplification) amplicons, unaffected by non-specific products resulting from primer dimers. However, low quenching efficiency by surrounding nucleobases leads to high background signal, posing significant challenges for visual inspection with the naked eye. [...] Read more.
The self-quenching fluorogenic probe facilitates precise identification of LAMP (loop-mediated isothermal amplification) amplicons, unaffected by non-specific products resulting from primer dimers. However, low quenching efficiency by surrounding nucleobases leads to high background signal, posing significant challenges for visual inspection with the naked eye. The present study aims to identify an oligonucleotide sequence that is complementary to the self-quenching fluorogenic probe, and to employ the fluorescence super-quenching mechanism of double-stranded DNA to establish a visualization system for the LAMP assay. The results indicated that the incorporation of a sequence fully complementary to the probe could significantly reduce the system’s background fluorescence (p < 0.05). When the melting temperature exceeds room temperature, truncating the complementary sequence from the 3′ end does not compromise the probe’s quenching efficiency. The LAMP visualization system, using a 10–13-base complementary sequence of the loop primer-based probe, could effectively minimize background fluorescence and yield straightforward visual results post-reaction. Applied to rainbow trout and Atlantic salmon detection, the system detected 1 pg DNA in a closed-tube format. In conclusion, a suitable complementary sequence can reduce the background fluorescence of the self-quenching fluorogenic probe. Employing this sequence alongside the self-quenching fluorogenic probe to develop a low-background fluorescence LAMP system demonstrates great potential for successful visual detection and holds considerable promotional merit. Full article
(This article belongs to the Special Issue Food Safety Detection Analysis and Sensors)
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18 pages, 3374 KiB  
Article
Prussian-Blue-Nanozyme-Enhanced Simultaneous Immunochromatographic Control of Two Relevant Bacterial Pathogens in Milk
by Olga D. Hendrickson, Nadezhda A. Byzova, Boris B. Dzantiev and Anatoly V. Zherdev
Foods 2024, 13(19), 3032; https://doi.org/10.3390/foods13193032 - 24 Sep 2024
Cited by 1 | Viewed by 927
Abstract
Salmonella typhimurium and Listeria monocytogenes are relevant foodborne bacterial pathogens which may cause serious intoxications and infectious diseases in humans. In this study, a sensitive immunochromatographic analysis (ICA) for the simultaneous detection of these two pathogens was developed. For this, test strips containing [...] Read more.
Salmonella typhimurium and Listeria monocytogenes are relevant foodborne bacterial pathogens which may cause serious intoxications and infectious diseases in humans. In this study, a sensitive immunochromatographic analysis (ICA) for the simultaneous detection of these two pathogens was developed. For this, test strips containing two test zones with specific monoclonal antibodies (MAb) against lipopolysaccharides of S. typhimurium and L. monocytogenes and one control zone with secondary antibodies were designed, and the double-assay conditions were optimized to ensure high analytical parameters. Prussian blue nanoparticles (PBNPs) were used as nanozyme labels and were conjugated with specific MAbs to perform a sandwich format of the ICA. Peroxidase-mimic properties of PBNPs allowed for the catalytic amplification of the colorimetric signal on test strips, enhancing the assay sensitivity. The limits of detection (LODs) of Salmonella and Listeria cells were 2 × 102 and 7 × 103 cells/mL, respectively. LODs were 100-fold less than those achieved due to the ICA based on the traditional gold label. The developed double ICA was approbated for the detection of bacteria in cow milk samples, which were processed by simple dilution by buffer before the assay. For S. typhimurium and L. monocytogenes, the recoveries from milk were 86.3 ± 9.8 and 118.2 ± 10.5% and correlated well with those estimated by the enzyme-linked immunosorbent assay as a reference method. The proposed approach was characterized by high specificity: no cross-reactivity with other bacteria strains was observed. The assay satisfies the requirements for rapid tests: a full cycle from sample acquisition to result assessment in less than half an hour. The developed ICA has a high application potential for the multiplex detection of other foodborne pathogens. Full article
(This article belongs to the Special Issue Food Safety Detection Analysis and Sensors)
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