Recent Advances in the Control of Ecto and Endo-Parasites in Ruminant from a Sustainable Perspective

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Parasitic Pathogens".

Deadline for manuscript submissions: closed (15 February 2023) | Viewed by 13651

Special Issue Editor


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Guest Editor
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Jiutepec 62550, Morelos, Mexico
Interests: ecto-parasites; endo-parasites; control strategies; sustainability; ruminants
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Special Issue Information

Dear Colleagues,

Both internal and external parasites are a worrying problem affecting animal health and limiting the productive potential of small and large ruminants, discouraging productive animal activity all over the world. Traditionally, the most common method of control of these parasitic diseases has based on the periodical and frequent administration of chemical drugs (CDs), which include anthelmintics and ixodicides in flocks and herds, and for many years, this method was considered a very useful and efficient measure of control of these parasites. In recent decades, however, the use of CDs against ecto and endoparasites has gained a bad reputation by producers of small and large ruminants worldwide for various reasons, for example, the presence of chemical drug residues in meat, milk or in sub-products derived from the livestock industry, which is considered a potential risk to public health. Additionally, the elimination of CD residues in feces from treated animals occasions an important contamination of soil and, similarly to the effect of chemical pesticides, affects beneficial soil microorganisms, which contribute to deteriorating soil fertility and environmental health. On the other hand, the imminent presence of resistance developed in parasites as a consequence of the continuous use of commercially available CDs causes a severe inefficacy of antiparasitic drugs that provokes an increase in parasitic populations together with their negative impact on animal health and on animal productivity. This demotivating situation has attracted great interest in workers around the world to search for other strategies of control of parasites with a focus on sustainability and innocuity. The present Special Issue aims to collect the most relevant scientific findings recently obtained on this important topic that allow minimizing the use of CDs whose negative consequences on human health, animal production, and the environment are an increasingly worrying problem. 

Dr. Pedro Mendoza De Gives
Guest Editor

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Keywords

  • ecto-parasites
  • endo-parasites
  • control strategies
  • sustainability
  • ruminants

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Published Papers (6 papers)

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Research

11 pages, 3089 KiB  
Article
Formulating Parasiticidal Fungi in Dried Edible Gelatins to Reduce the Risk of Infection by Trichuris sp. among Continuous Grazing Bison
by Rami Salmo, Cándido Viña, Izaro Zubiria, José Ángel Hernández Malagón, Jaime M. Sanchís, Cristiana Cazapal, María Sol Arias, Rita Sánchez-Andrade and Adolfo Paz-Silva
Pathogens 2024, 13(1), 82; https://doi.org/10.3390/pathogens13010082 - 17 Jan 2024
Viewed by 1141
Abstract
Control of infection by gastrointestinal nematodes remains a big problem in ruminants under continuous grazing. For the purpose of decreasing the risk of infection by Trichuris sp. in captive bison (Bison bison) always maintained in the same plot, dried gelatins having [...] Read more.
Control of infection by gastrointestinal nematodes remains a big problem in ruminants under continuous grazing. For the purpose of decreasing the risk of infection by Trichuris sp. in captive bison (Bison bison) always maintained in the same plot, dried gelatins having ≥106 chlamydospores of both Mucor circinelloides and Duddingtonia flagrans were given to them for one week, and at the end, fecal samples (FF) collected each week for four weeks were analyzed immediately. Feces taken one week prior to gelatin administration served as controls (CF). Eggs of Trichuris sp. were sorted into non-viable and viable, then classified into viable undeveloped (VU), viable with cellular development (VCD), or viable infective (VI). Ovistatic and ovicidal effects were determined throughout the study. In FF, viability of Trichuris eggs decreased between 9% (first week) and 57% (fourth week), egg development was delayed during the first two weeks, and VI percentages were significantly lower than in CF (p = 0.001). It is concluded that the preparation of gelatins with chlamydospores of parasiticidal fungi and their subsequent dehydration offer an edible formulation that is ready to use, stress-free to supply, and easy to store, as well as being well-accepted by ruminants and highly efficient to reduce the risk of Trichuris sp. infection among animals under continuous grazing regimes. Full article
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10 pages, 885 KiB  
Article
Coffee Pulp: A Natural Alternative for Control of Resistant Nematodes in Small Ruminants
by Gabino López-Rodríguez, Adrian Zaragoza-Bastida, David Emanuel Reyes-Guerrero, Agustín Olmedo-Juárez, Benjamín Valladares-Carranza, Luis Fernando Vega-Castillo and Nallely Rivero-Perez
Pathogens 2023, 12(1), 124; https://doi.org/10.3390/pathogens12010124 - 11 Jan 2023
Viewed by 2415
Abstract
Goat production in Mexico is an important economic activity that is affected by different gastrointestinal nematode (GIN) species. GINs resistant to commercial anthelmintics have been reported. Plant extracts or agro-industrial by-products, such as coffee pulp, have been proposed as control alternatives, given their [...] Read more.
Goat production in Mexico is an important economic activity that is affected by different gastrointestinal nematode (GIN) species. GINs resistant to commercial anthelmintics have been reported. Plant extracts or agro-industrial by-products, such as coffee pulp, have been proposed as control alternatives, given their secondary metabolite content. The aim of the present study was to determine the anthelmintic activity of the hydroalcoholic extract of coffee pulp against benzimidazole-resistant GINs. Stool samples were collected from goats, from which GIN eggs were identified and quantified. Molecular techniques confirmed the genus of GINs and their benzimidazole resistance profile. The percentage of egg hatching inhibition (% EHI) and larval mortality (% LM) with the hydroalcoholic extract of coffee pulp was determined at concentrations from 200 to 0.39 mg/mL. The genera Haemonchus spp. and Trichostrongylus spp. were identified, and the presence of the β-tubulin gene mutation, associated with benzimidazole (BZ) resistance, was determined. Hydroalcoholic extract of coffee pulp inhibited 100% of egg hatching at 200 and 100 mg/mL, with no larvicidal effect at the evaluated concentrations. Full article
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11 pages, 700 KiB  
Article
Anthelmintic Efficacy in Sheep and Goats under Different Management and Deworming Systems in the Region of Lisbon and Tagus Valley, Portugal
by Maria Inês Antunes, Miguel Saraiva Lima, George Stilwell, Maria Inês Romeiras, Luís Fragoso and Luís Manuel Madeira de Carvalho
Pathogens 2022, 11(12), 1457; https://doi.org/10.3390/pathogens11121457 - 1 Dec 2022
Cited by 4 | Viewed by 2589
Abstract
Infections caused by gastrointestinal parasites have been described worldwide as one of the most important issues impacting small ruminant production. The systematic administration of anthelmintic (AH) drugs without following good practice principles has led to an increase in anthelmintic resistance (AR). There is [...] Read more.
Infections caused by gastrointestinal parasites have been described worldwide as one of the most important issues impacting small ruminant production. The systematic administration of anthelmintic (AH) drugs without following good practice principles has led to an increase in anthelmintic resistance (AR). There is scarce information regarding AH efficacy in small ruminants in Portugal. This study aimed to characterize by in vivo methods the presence and level of AR in four farms in the region of Lisbon and Tagus Valley. All four farms kept small herds in extensive management systems and used different deworming protocols. The active substances used were fenbendazole and a combination of mebendazole plus closantel in a dosage according to the manufacturers’ instructions. On day 0 (T0), fecal samples were collected from all study animals, and animals in the treatment group (n = 40) were dewormed with the AH previously chosen by the assistant veterinarian. Animals in the control group (n = 30) did not receive any AH drug. The fecal sample collection was repeated on day 15 (T15), and the control group was treated. Egg counts were performed using the McMaster method for the eggs per gram (EPG), and AR was evaluated by the fecal egg count reduction test (FECRT) with a 95% confidence level (CL). The results from this experiment indicated that the four farms presented AR with two farms to fenbendazole (FECRT of 48% and 85%) and two farms to mebendazole plus closantel (FECRT of 66% and 79%). These results indicate that the gastrointestinal parasites of the four studied Portuguese farms are resistant to benzimidazoles, which suggests an increase in AR regarding nematodes in small ruminant production systems in Portugal. Full article
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11 pages, 513 KiB  
Article
In Vitro Anthelmintic Activity of a Hydroalcoholic Extract from Guazuma ulmifolia Leaves against Haemonchus contortus
by Guillermo Reséndiz-González, Rosa Isabel Higuera-Piedrahita, Alejandro Lara-Bueno, Roberto González-Gardúño, Jorge Alberto Cortes-Morales, Manasés González-Cortazar, Pedro Mendoza-de Gives, Sara Guadalupe Romero-Romero and Agustín Olmedo-Juárez
Pathogens 2022, 11(10), 1160; https://doi.org/10.3390/pathogens11101160 - 7 Oct 2022
Cited by 6 | Viewed by 2135
Abstract
The purpose of the present study was to assess the ovicidal and larvicidal activity of a hydroalcoholic extract (HAE) and their fractions (aqueous, Aq-F and organic, EtOAc-F) from Guazuma ulmifolia leaves using Haemonchus contortus as a biological model. The egg hatching inhibition (EHI) [...] Read more.
The purpose of the present study was to assess the ovicidal and larvicidal activity of a hydroalcoholic extract (HAE) and their fractions (aqueous, Aq-F and organic, EtOAc-F) from Guazuma ulmifolia leaves using Haemonchus contortus as a biological model. The egg hatching inhibition (EHI) and larval mortality against infective larvae (L3) tests were used to determine the anthelmintic effect of the treatments. The extract and fractions were tested at different concentrations against eggs and L3. Additionally, distilled water and methanol were used as negative controls and ivermectin as a positive control. The extract and fractions were subjected to HPLC analysis to identify the major compounds. The HAE displayed the highest ovicidal activity (100% EHI at 10 mg/mL). Fractionation of the HA extract allowed increasing the nematicidal effect in the EtOAc-F (100% EHI at 0.62 mg/mL and 85.35% mortality at 25 mg/mL). The phytochemical analysis of the extract and fractions revealed the presence of kaempferol, ethyl ferulate, ethyl coumarate, flavonol, luteolin, ferulic acid, luteolin rhamnoside, apigenin rutinoside, coumaric acid derivative, luteolin glucoside, and quercetin glucoside. These results suggest that G. ulmifolia leaves could be potential candidates for the control of H. contortus or other gastrointestinal parasitic nematodes. Full article
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13 pages, 2625 KiB  
Article
Protein Supplementation as a Nutritional Strategy to Reduce Gastrointestinal Nematodiasis in Periparturient and Lactating Pelibuey Ewes in a Tropical Environment
by Yoel López-Leyva, Roberto González-Garduño, Alvar Alonzo Cruz-Tamayo, Javier Arece-García, Maximino Huerta-Bravo, Rodolfo Ramírez-Valverde, Glafiro Torres-Hernández and M. Eugenia López-Arellano
Pathogens 2022, 11(8), 941; https://doi.org/10.3390/pathogens11080941 - 19 Aug 2022
Cited by 3 | Viewed by 1637
Abstract
The study was carried out to evaluate the effect of energy and protein supplementation on parasitological and hematological response during peripartum and lactation of productive and non-productive Pelibuey ewes in a tropical environment. Forty-eight Pelibuey ewes aged 3–5 years and with a body [...] Read more.
The study was carried out to evaluate the effect of energy and protein supplementation on parasitological and hematological response during peripartum and lactation of productive and non-productive Pelibuey ewes in a tropical environment. Forty-eight Pelibuey ewes aged 3–5 years and with a body weight of 31 ± 5 kg were used. Four groups of 12 ewes, including non-pregnant and productive ewes, were formed. A factorial treatment design was formulated, where two levels of energy (low, 9.6 MJ/kg, n = 24; and high, 10.1 MJ/kg, n = 24) and two levels of protein (high, 15% crude protein in diet, n = 24; and low, 8% crude protein in diet, n = 24) were studied. Fecal and blood samples were collected to determine the fecal egg count (FEC) of gastrointestinal nematodes (GIN), packed cell volume (PCV) and peripheral eosinophil (EOS) count. These variables were rearranged with respect to the lambing date in a retrospective study. The high dietary protein level had a significant effect on reducing the FEC and increasing the PCV of ewes during lactation, in comparison with animals fed with the low protein level. Differences in the study variables were attributed to physiological stage. Lactating ewes showed the highest FEC values (2709 ± 359 EPG), the lowest PCV values (21.9 ± 0.7%) and the lowest EOS (0.59 ± 0.6 Cells × 103 µL). It is concluded that high levels of dietary protein improve the hematological response and reduce the FEC in Pelibuey ewes under grazing conditions. The non-pregnant ewes maintained some resilience and resistance to GIN infection compared to productive ewes. Full article
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12 pages, 1503 KiB  
Article
Genome-Wide Association Study for Haemonchus contortus Resistance in Morada Nova Sheep
by Simone Cristina Méo Niciura, Magda Vieira Benavides, Cintia Hiromi Okino, Adriana Mercia Guaratini Ibelli, Alessandro Pelegrine Minho, Sergio Novita Esteves and Ana Carolina de Souza Chagas
Pathogens 2022, 11(8), 939; https://doi.org/10.3390/pathogens11080939 - 19 Aug 2022
Cited by 10 | Viewed by 2250
Abstract
Among the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of [...] Read more.
Among the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case–control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, OAR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification. Full article
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