Molecular Markers and Molecular Breeding in Horticultural Plants, 2nd Edition

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 20 February 2025 | Viewed by 1913

Special Issue Editors


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Guest Editor
Department of Horticultural Bioscience, Pusan National University, Miryang 50463, Republic of Korea
Interests: plant genetics; plant breeding; molecular breeding; molecular markers; genomics; vegetables; tomato; cucurbits; disease resistance; horticultural traits
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Guest Editor
Department of Bioresources Engineering, Sejong University, Seoul 05006, Republic of Korea
Interests: plant genetics and breeding
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Special Issue Information

Dear Colleagues,

Plant molecular breeding, including marker-assisted breeding and genetic engineering, has experienced remarkable innovations and advances during the last few decades. The availability of high-throughput platforms focused on different omics—from genomics to metabolomics—has made important information related to key genes and pathways associated with important agronomical traits available. Molecular markers and molecular-assisted breeding are becoming increasingly important. This Special Issue intends to report the recent advances in breeding strategies focused on innovative analysis from different omics approaches. Approaches focused on genetics (genotyping and phenotyping), genomics (the identification of genes directly linked with agronomical traits, regulatory elements, or functional polymorphisms), and transcriptomics (the identification of key gene(s) and the validation of their involvement in the expression of specific traits) will be considered.

Dr. Younghoon Park
Dr. Sung-Chur Sim
Guest Editors

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Keywords

  • molecular markers
  • molecular breeding
  • horticultural plants
  • quality improvement

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Published Papers (2 papers)

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Research

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12 pages, 3960 KiB  
Article
Development of Cost-Effective SNP Markers for Genetic Variation Analysis and Variety Identification in Cultivated Pears (Pyrus spp.)
by Jae-Hun Heo, Jeyun Yeon, Jin-Kee Jung, Il Sheob Shin and Sung-Chur Sim
Plants 2024, 13(18), 2600; https://doi.org/10.3390/plants13182600 - 18 Sep 2024
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Abstract
Pear (Pyrus spp.) is a major fruit crop in the Rosaceae family, and extensive efforts have been undertaken to develop elite varieties. With advances in genome sequencing technologies, single-nucleotide polymorphisms (SNPs) are commonly used as DNA markers in crop species. In this [...] Read more.
Pear (Pyrus spp.) is a major fruit crop in the Rosaceae family, and extensive efforts have been undertaken to develop elite varieties. With advances in genome sequencing technologies, single-nucleotide polymorphisms (SNPs) are commonly used as DNA markers in crop species. In this study, a large-scale discovery of SNPs was conducted using genotyping by sequencing in a collection of 48 cultivated pear accessions. A total of 256,538 confident SNPs were found on 17 chromosomes, and 288 SNPs were filtered based on polymorphic information content, heterozygosity rate, and genome distribution. This subset of SNPs was used to genotype an additional 144 accessions, consisting of P. pyrifolia (53), P. ussuriensis (27), P. bretschneideri (19), P. communis (26), interspecific hybrids (14), and others (5). The 232 SNPs with reliable polymorphisms revealed genetic variations between and within species in the 192 pear accessions. The Asian species (P. pyrifolia, P. ussuriensis, and P. bretschneideri) and interspecific hybrids were genetically differentiated from the European species (P. communis). Furthermore, the P. pyrifolia population showed higher genetic diversity relative to the other populations. The 232 SNPs and four subsets (192, 96, 48, and 24 SNPs) were assessed for variety identification. The 192 SNP subset identified 173 (90.1%) of 192 accessions, which was comparable to 175 (91.1%) from the 232 SNPs. The other three subsets showed 81.8% (24 SNPs) to 87.5% (96 SNPs) identification rates. The resulting SNPs will be a useful resource to investigate genetic variations and develop an efficient DNA barcoding system for variety identification in cultivated pears. Full article
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25 pages, 1579 KiB  
Review
Advances in Soybean Genetic Improvement
by Adriana Vargas-Almendra, Roberto Ruiz-Medrano, Leandro Alberto Núñez-Muñoz, José Abrahán Ramírez-Pool, Berenice Calderón-Pérez and Beatriz Xoconostle-Cázares
Plants 2024, 13(21), 3073; https://doi.org/10.3390/plants13213073 - 31 Oct 2024
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Abstract
The soybean (Glycine max) is a globally important crop due to its high protein and oil content, which serves as a key resource for human and animal nutrition, as well as bioenergy production. This review assesses recent advancements in soybean genetic [...] Read more.
The soybean (Glycine max) is a globally important crop due to its high protein and oil content, which serves as a key resource for human and animal nutrition, as well as bioenergy production. This review assesses recent advancements in soybean genetic improvement by conducting an extensive literature analysis focusing on enhancing resistance to biotic and abiotic stresses, improving nutritional profiles, and optimizing yield. We also describe the progress in breeding techniques, including traditional approaches, marker-assisted selection, and biotechnological innovations such as genetic engineering and genome editing. The development of transgenic soybean cultivars through Agrobacterium-mediated transformation and biolistic methods aims to introduce traits such as herbicide resistance, pest tolerance, and improved oil composition. However, challenges remain, particularly with respect to genotype recalcitrance to transformation, plant regeneration, and regulatory hurdles. In addition, we examined how wild soybean germplasm and polyploidy contribute to expanding genetic diversity as well as the influence of epigenetic processes and microbiome on stress tolerance. These genetic innovations are crucial for addressing the increasing global demand for soybeans, while mitigating the effects of climate change and environmental stressors. The integration of molecular breeding strategies with sustainable agricultural practices offers a pathway for developing more resilient and productive soybean varieties, thereby contributing to global food security and agricultural sustainability. Full article
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