Submit to Special Issue Submit Abstract to Special Issue Review for Plants Propose a Special Issue

Journal Menu

Journal Browser

► Journal Browser

Medicinal Plants: Advances in Phytochemistry and Ethnobotany II

Print Special Issue Flyer
Special Issue Editors
Special Issue Information
Keywords
Benefits of Publishing in a Special Issue
Related Special Issue
Published Papers

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Phytochemistry".

Deadline for manuscript submissions: 28 February 2025 | Viewed by 12607

Share This Special Issue

Special Issue Editors

Prof. Dr. Dâmaris Silveira

E-Mail Website
Guest Editor

Laboratory of Natural Products (LaProNat), Faculty of Health Sciences, University of Brasilia (UnB), Campus Universitário Darcy Ribeiro, Asa Norte, Brasilia, Brazil
Interests: natural products chemistry; herbal medicines; regulatory issues; Cerrado plant species
Special Issues, Collections and Topics in MDPI journals

Dr. Fabio Boylan

E-Mail Website
Guest Editor

School of Pharmacy and Pharmaceutical Sciences, Faculty of Health Sciences, Panoz Institute, Trinity College Dublin (TCD), Dublin, Ireland
Interests: natural products chemistry; medicinal plants pharmacology; ethnopharmacology; chemotaxonomy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Ethnobotanical information and phytochemical studies are usually the first steps taken to validate the ethnomedicinal use of a plant species, together with in vitro and in vivo preclinical assays. Although preliminary, all data obtained in these initial stages are important to define the next steps towards the potential development of a herbal medicine. For native and endemic species, research in these areas is sometimes key to avoid the destruction of the plant population or the extinction of the species. Additionally, in this sense, classic and modern techniques, together with the use of artificial intelligence, metabolomic analysis and system pharmacology, are integrated in order to allow a full understanding of a herbal medicine. Last year, in the first edition of the Special Issue ‘Medicinal Plants: Advances in Phytochemistry and Ethnobotany’, 27 research groups worldwide submitted original papers or reviews on several aspects of the use, chemistry, pharmacology and quality control of medicinal plants, and among them, thirteen papers were published. Research and attempts to fully understand medicinal plants are an endless task, especially considering their different traditional uses. Therefore, a second issue on this same relevant theme is now presented, and we invite researchers to share their findings on all aspects of ethnobotany as well as chemistry, toxicity, biological activity, AI and regulatory issues linked to medicinal plants and their development into new medicines.

Prof. Dr. Dâmaris Silveira
Dr. Fabio Boylan
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

ethnobotanical survey
traditional medicine
phytochemistry investigation
regulatory issues
biological activity assays

Benefits of Publishing in a Special Issue

Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Related Special Issue

Medicinal Plants: Advances in Phytochemistry and Ethnobotany in Plants (14 articles)

Published Papers (7 papers)

Download All Papers

Order results

Result details

Show export options Show export options

Select all

Export citation of selected articles as:

Research

19 pages, 3747 KiB

Open AccessArticle

Furostanol Saponins and Ecdysteroids from Plants of the Genus Helleborus as Phagostimulants and Predator Deterrents for Larvae of Two Monophadnus Sawfly Species

by Jose M. Prieto, Alison M. Barker, Urs Schaffner, Joëlle Quetin-Leclercq, Alessandra Braca and Jean-Luc Boevé

Plants 2024, 13(16), 2230; https://doi.org/10.3390/plants13162230 - 12 Aug 2024

Viewed by 760

Abstract

Sawfly species of the genus Monophadnus are specialised on Ranunculaceae plants from which the larvae can sequester furostanol saponins into the haemolymph, mainly (25R)-26-[(α-L-rhamnopyranosyl)oxy]-22α-methoxyfurost-5-en-3β-yl-O-β-D-glucopyranosyl-(1→3)-O-[6-acetyl-β-D-glucopyranosyl-(1→3)]-O-β-D-glucopyranoside (compound 1). In this work, TLC, GC-MS, and HPLC-DAD-ESI/MS analyses together with feeding, repeated simulated attacks, and ant deterrence bioassays were conducted to extend the chemoecological knowledge about two sawfly species specialised on H. foetidus L. (Monophadnus species A) and H. viridis L. (Monophadnus species B). Larvae of Monophadnus species B were mostly feeding on the squares treated with the n-butanol fraction from H. foetidus, compound 1 being its primary non-nutritional stimulant. In contrast, all H. viridis fractions stimulated feeding, with n-hexane marginally more active. β-sitosterol within n-hexane was determined as the nutritional stimulant. Quantitative analyses demonstrated that leaves of H. viridis but not H. foetidus contain the ecdysteroids 20-hydroxyecdysone and polypodine B. Moreover, the haemolymph of Monophadnus species B larvae reared on H. viridis contained the glycosides of polypodine B and 20-hydroxyecdysone at a concentration of 2.5 to 6.8 µmol/g fresh weight of haemolymph. This concentration is several thousand times higher than the concentration range of the aglycones in their host plant (3.63 × 10⁻⁴ to 2.23 × 10⁻⁴ µmol total ecdysteroids/g fresh weight of leaves), suggesting bioaccumulation. The larvae of both species fed on H. foetidus do not show any traces of ecdysteroids in their haemolymph, indicating a facultative role of these compounds in their defence as well as their inability to endogenously synthesise these compounds. The haemolymph containing ecdysteroids was a significant feeding deterrent against Myrmica rubra L. ant workers (one of their natural predators) at 0.8 mg/mL. The larvae kept effective deterrent levels of glycosylated ecdysteroids (≅175 mM) between simulated attacks on days 1 and 2, but the levels clearly decreased on day 3 (≅75 mM). Most larvae (89%) survived a first attack but only 23% a consecutive second one. As a conclusion, we report for the first time that two Monophadnus species feeding on H. viridis sequester phytoecdysteroids into the larval haemolymph in the form of glycosides. In addition, compound 1 possesses defensive and phagostimulant activities, and we present evidence for a combined effect of furostanol saponins and ecdysteroids as repellents against ants. Full article

(This article belongs to the Special Issue Medicinal Plants: Advances in Phytochemistry and Ethnobotany II)

► Show Figures

Graphical abstract

19 pages, 2110 KiB

Open AccessArticle

Influence of Sucrose and Activated Charcoal on Phytochemistry and Vegetative Growth in Zephyranthes irwiniana (Ravenna) Nic. García (Amaryllidaceae)

by Bertholdo Dewes Neto, Kicia Karinne Pereira Gomes-Copeland, Dâmaris Silveira, Sueli Maria Gomes, Julia Marina Muller Craesmeyer, Daniela Aparecida de Castro Nizio and Christopher William Fagg

Plants 2024, 13(5), 569; https://doi.org/10.3390/plants13050569 - 20 Feb 2024

Viewed by 1384

Abstract

Zephyranthes irwiniana (Ravenna) Nic. García is an endemic, red list threatened species from the Brazilian savanna (Cerrado) with pharmacological potential to treat the symptoms of Alzheimer’s Disease (AD). This work analyzed the vegetative growth and phytochemistry of its potential compounds, in response to variations in sucrose concentration and activated carbon (AC). Seeds were germinated in vitro and in the greenhouse. The in vitro bulbs were separated in six treatments with different sucrose concentrations (30, 45 and 60 gL⁻¹) and/or AC (1 gL⁻¹). Biomass increases in individuals grown in the greenhouse were higher than those cultivated in vitro. Sucrose concentration significantly increased biomass and root number. AC had a positive influence on leaf and root size, and a negative influence on root number. GC–MS analyses indicated great variation in the abundance of α-terpenyl-acetate, ethyl linoleate, clionasterol and lycorine between treatments, with maximum concentrations of 53.06%, 38.68, 14.34% and 2.57%, respectively. Histolocalization tests indicated the presence of alkaloids in the leaf chlorenchyma and bulb cataphylls. Finally, the present study provided new evidence that the constitution of the culture medium directly influences the vegetative growth and phytochemistry of this species, providing a good medium condition for propagating the species under threat. Full article

(This article belongs to the Special Issue Medicinal Plants: Advances in Phytochemistry and Ethnobotany II)

► Show Figures

Figure 1

12 pages, 1503 KiB

Open AccessArticle

Vasodilation and Blood Pressure-Lowering Effect of 3-Demethyl-2-Geranyl-4-Prenylbellidifoline, a Xanthone Obtained from Garcinia achachairu, in Hypertensive Rats

by Luísa Nathália Bolda Mariano, Rita de Cássia Vilhena da Silva, Rivaldo Niero, Valdir Cechinel Filho, José Eduardo da Silva-Santos and Priscila de Souza

Plants 2024, 13(4), 528; https://doi.org/10.3390/plants13040528 - 15 Feb 2024

Viewed by 1290

Abstract

3-demethyl-2-geranyl-4-prenylbellidifoline (DGP), a natural xanthone isolated from Garcinia achachairu, has previously demonstrated remarkable diuretic and renal protective actions. The present study expands its actions on the cardiovascular system by evaluating its vasorelaxant and blood pressure-lowering effects in spontaneously hypertensive rats (SHRs). Aortic endothelium-intact (E+) preparations of SHRs pre-contracted by phenylephrine and exposed to cumulative concentrations of G. achachairu extract, fractions, and DGP exhibited a significant relaxation compared to vehicle-only exposed rings. The non-selective muscarinic receptor antagonist (atropine), the non-selective inhibitor of nitric oxide synthase (L-NAME), as well as the inhibitor of soluble guanylate cyclase (ODQ) altogether avoided DGP-induced relaxation. Tetraethylammonium (small conductance Ca²⁺-activated K⁺ channel blocker), 4-aminopyridine (a voltage-dependent K⁺ channel blocker), and barium chloride (an influx-rectifying K⁺ channel blocker) significantly reduced DGP capacity to induce relaxation without the interference of glibenclamide (an ATP-sensitive inward rectifier 6.1 and 6.2 K⁺ channel blocker). Additionally, administration of DGP, 1 mg/kg i.v., decreased the mean, systolic, and diastolic arterial pressures, and the heart rate of SHRs. The natural xanthone DGP showed promising potential as an endothelium-dependent vasorelaxant, operating through the nitric oxide pathway and potassium channels, ultimately significantly reducing blood pressure in hypertensive rats. Full article

(This article belongs to the Special Issue Medicinal Plants: Advances in Phytochemistry and Ethnobotany II)

► Show Figures

Figure 1

17 pages, 3116 KiB

Open AccessArticle

Exploring the Anticancer Potential of Traditional Thai Medicinal Plants: A Focus on Dracaena loureiri and Its Effects on Non-Small-Cell Lung Cancer

by Xiaomin Huang, Punnida Arjsri, Kamonwan Srisawad, Supachai Yodkeeree and Pornngarm Dejkriengkraikul

Plants 2024, 13(2), 290; https://doi.org/10.3390/plants13020290 - 18 Jan 2024

Cited by 6 | Viewed by 2295

Abstract

Non-small-cell lung cancer (NSCLC) is renowned for its aggressive and highly metastatic nature. In recent years, there has been a surge in interest regarding the therapeutic potential of traditional medicinal plants. Dracaena loureirin (D. loureirin), Ficus racemosa Linn. (F. racemosa), and Harrisonia perforata (Blanco) Merr. (H. perforata) are prominent traditional medicinal herbs in Thailand, recognized for their diverse biological activities, including antipyretic and anti-inflammatory effects. However, their prospective anti-cancer properties against NSCLC remain largely unexplored. This study aimed to evaluate the anti-cancer attributes of ethanolic extracts obtained from D. loureiri (DLEE), F. racemosa (FREE), and H. perforata (HPEE) against the A549 lung adenocarcinoma cell lines. Sulforhodamine B (SRB) assay results revealed that only DLEE exhibited cytotoxic effects on A549 cells, whereas FREE and HPEE showed no such cytotoxicity. To elucidate the anti-cancer mechanisms of DLEE, cell cycle and apoptosis assays were performed. The findings demonstrated that DLEE inhibited cell proliferation and induced cell cycle arrest at the G0/G1 phase in A549 cells through the downregulation of key cell cycle regulator proteins, including cyclin D1, CDK-2, and CDK-4. Furthermore, DLEE treatment facilitated apoptosis in A549 cells by suppressing anti-apoptotic proteins (Bcl-2, Bcl-xl, and survivin) and enhancing apoptotic proteins (cleaved-caspase-3 and cleaved-PARP-1). In summary, our study provides novel insights into the significant anti-cancer properties of DLEE against A549 cells. This work represents the first report suggesting that DLEE has the capability to impede the growth of A549 lung adenocarcinoma cells through the induction of apoptosis. Full article

(This article belongs to the Special Issue Medicinal Plants: Advances in Phytochemistry and Ethnobotany II)

► Show Figures

Figure 1

29 pages, 1574 KiB

Open AccessArticle

A Comprehensive Study on Lathyrus tuberosus L.: Insights into Phytochemical Composition, Antimicrobial Activity, Antioxidant Capacity, Cytotoxic, and Cell Migration Effects

by Rita Jakabfi-Csepregi, Ágnes Alberti, Csenge Anna Felegyi-Tóth, Tamás Kőszegi, Szilvia Czigle and Nóra Papp

Plants 2024, 13(2), 232; https://doi.org/10.3390/plants13020232 - 14 Jan 2024

Cited by 3 | Viewed by 1653

Abstract

In this study, in vitro antioxidant, antimicrobial, cytotoxic, and cell migration effects of phenolic compounds of Lathyrus tuberosus leaves, known in the Transylvanian ethnomedicine, were investigated. Ultra-high-performance liquid chromatography-tandem mass spectrometry was employed for the analysis of the ethanolic and aqueous extracts. The antimicrobial properties were determined using a conventional microdilution technique. Total antioxidant capacity techniques were used using cell-free methods and cell-based investigations. Cytotoxic effects were conducted on 3T3 mouse fibroblasts and HaCaT human keratinocytes using a multiparametric method, assessing intracellular ATP, total nucleic acid, and protein levels. Cell migration was visualized by phase-contrast microscopy, employing conventional culture inserts to make cell-free areas. Together, 93 polyphenolic and monoterpenoid compounds were characterized, including flavonoid glycosides, lignans, hydroxycinnamic acid, and hydroxybenzoic acid derivatives, as well as iridoids and secoiridoids. The ethanolic extract showed high antioxidant capacity and strong antimicrobial activity against Bacillus subtilis (MIC₈₀ value: 354.37 ± 4.58 µg/mL) and Streptococcus pyogenes (MIC₈₀ value: 488.89 ± 4.75 µg/mL). The abundance of phenolic compounds and the results of biological tests indicate the potential for L. tuberosus to serve as reservoirs of bioactive compounds and to be used in the development of novel nutraceuticals. Full article

(This article belongs to the Special Issue Medicinal Plants: Advances in Phytochemistry and Ethnobotany II)

► Show Figures

Figure 1

29 pages, 6315 KiB

Open AccessArticle

Sustainable Pest Management Using Novel Nanoemulsions of Honeysuckle and Patchouli Essential Oils against the West Nile Virus Vector, Culex pipiens, under Laboratory and Field Conditions

by Wafaa M. Hikal, Mohamed M. Baz, Mohammed Ali Alshehri, Omar Bahattab, Rowida S. Baeshen, Abdelfattah M. Selim, Latifah Alhwity, Rabaa Bousbih, Maha Suleiman Alshourbaji and Hussein A. H. Said-Al Ahl

Plants 2023, 12(21), 3682; https://doi.org/10.3390/plants12213682 - 25 Oct 2023

Cited by 3 | Viewed by 2130

Abstract

Essential oils are natural plant products that are very interesting, as they are important sources of biologically active compounds. They comprise eco-friendly alternatives to mosquito vector management, particularly essential oil nanoemulsion. Therefore, the aim of this study is to evaluate the effectiveness of 16 selected essential oils (1500 ppm) in controlling mosquitoes by investigating their larvicidal effects against the larvae and adults of the West Nile virus vector Culex pipiens L. (Diptera: Culicidae); the best oils were turned into nanoemulsions and evaluated under laboratory and field conditions. The results show that honeysuckle (Lonicera caprifolium) and patchouli (Pogostemon cablin) essential oils were more effective in killing larvae than the other oils (100% mortality) at 24 h post-treatment. The nanoemulsions of honeysuckle (LC₅₀ = 88.30 ppm) and patchouli (LC₅₀ = 93.05 ppm) showed significantly higher larvicidal activity compared with bulk honeysuckle (LC₅₀ = 247.72 ppm) and patchouli (LC₅₀ = 276.29 ppm) oils. L. caprifolium and P. cablin (100% mortality), followed by Narcissus tazetta (97.78%), Rosmarinus officinalis (95.56%), and Lavandula angustifolia (95.55%), were highly effective oils in killing female mosquitoes, and their relative efficacy at LT₅₀ was 5.5, 5.3, 5.8, 4.1, and 3.2 times greater, respectively, than Aloe vera. The results of the field study show that the honeysuckle and patchouli oils and their nanoemulsions reduced densities to 89.4, 86.5, 98.6, and 97.0% at 24 h post-treatment, respectively, with persistence for eight days post-treatment in pools. Nano-honeysuckle (100% mortality) was more effective than honeysuckle oils (98.0%). Our results show that honeysuckle and patchouli oils exhibited promising larvicidal and adulticidal activity of C. pipiens. Full article

(This article belongs to the Special Issue Medicinal Plants: Advances in Phytochemistry and Ethnobotany II)

► Show Figures

Figure 1

20 pages, 7606 KiB

Open AccessArticle

Hepatoprotective Effects of Four Brazilian Savanna Species on Acetaminophen-Induced Hepatotoxicity in HepG2 Cells

by Gislane dos Santos Ribeiro, Diegue Henrique Nascimento Martins, João Victor Dutra Gomes, Noel William Davies, Christopher William Fagg, Luiz Alberto Simeoni, Mauricio Homem-de-Mello, Pérola Oliveira Magalhães, Dâmaris Silveira and Yris Maria Fonseca-Bazzo

Plants 2023, 12(19), 3393; https://doi.org/10.3390/plants12193393 - 26 Sep 2023

Viewed by 1844

Abstract

We investigated four Cerrado plant species, i.e., Cheiloclinium cognatum (Miers) A.C.Sm, Guazuma ulmifolia Lam., Hancornia speciosa Gomes, and Hymenaea stigonocarpa Mart. ex Hayne, against acetaminophen toxicity using an in vitro assay with HepG2 cells. The activity against acetaminophen toxicity was evaluated using different protocols, i.e., pre-treatment, co-treatment, and post-treatment of the cells with acetaminophen and the plant extracts. HepG2 cell viability after treatment with acetaminophen was 39.61 ± 5.59% of viable cells. In the pre-treatment protocol, the extracts could perform protection with viability ranging from 50.02 ± 15.24% to 78.75 ± 5.61%, approaching the positive control silymarin with 75.83 ± 5.52%. In the post-treatment protocol, all extracts and silymarin failed to reverse the acetaminophen damage. In the co-treatment protocol, the extracts showed protection ranging from 50.92 ± 11.14% to 68.50 ± 9.75%, and silymarin showed 77.87 ± 4.26%, demonstrating that the aqueous extracts of the species also do not increase the toxic effect of acetaminophen. This protection observed in cell viability was accompanied by a decrease in ROS. The extracts’ hepatoprotection can be related to antioxidant compounds, such as rutin and mangiferin, identified using HPLC-DAD and UPLC-MS/MS. The extracts were shown to protect HepG2 cells against future APAP toxicity and may be candidates for supplements that could be used to prevent liver damage. In the concomitant treatment using the extracts with APAP, it was demonstrated that the extracts do not present a synergistic toxicity effect, with no occurrence of potentiation of toxicity. The extracts showed considerable cytoprotective effects and important antioxidant characteristics. Full article

(This article belongs to the Special Issue Medicinal Plants: Advances in Phytochemistry and Ethnobotany II)

► Show Figures

Journal Menu

Journal Browser

Medicinal Plants: Advances in Phytochemistry and Ethnobotany II

Share This Special Issue

Special Issue Editors

Special Issue Information

Keywords

Benefits of Publishing in a Special Issue

Related Special Issue

Published Papers (7 papers)

Research

Further Information

Guidelines

MDPI Initiatives

Follow MDPI