Plant-Based Expression System

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (31 March 2022) | Viewed by 4753

Special Issue Editors


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Guest Editor
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
Interests: plant biotechnology; plant gene expression; plant molecular farming; plant-derived vaccine; virus-like particle; plant cell culture; plant regeneration
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Guest Editor
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
Interests: plant biotechnology; plant transformation; plant genome editing; protoplast culture; virus-like particles; plant molecular farming

Special Issue Information

Dear Colleagues,

Climate change, which in recent years has come close to catastrophic levels worldwide, is once again reminding us of the importance of plants. Plants are one of the biggest sources for potential biopharmaceuticals due to their ability to synthesize secondary metabolites. Additionally, plants are considered an attractive alternative production host for heterologous nonplant proteins including antibodies, subunit vaccines, and therapeutic enzymes. Cell culture systems are preferred for the production of secondary metabolites, whereas in the case of molecular farming, transient expression systems using Nicotiana benthamiana are most often used. Plant systems allow large-scale production which has low start-up costs, is safe and free of mammalian viral pathogens, and economical compared to other biological systems.

In this Special Issue, we would like to introduce various expression methods and practical examples for the production of biomaterials using these advantages of plants. Possible topics in this issue range from laboratory research to practical cases related to the expression and production of biomaterials. Moreover, this issue will highlight recent advances that have been achieved in plant-based production platforms in terms of plant species, expression strategies, product yields, and downstream processing development. 

I would like to thank all authors for their contributions to this Special issue.

Dr. Hyun-Soon Kim
Dr. Ki-Beom Moon
Guest Editors

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Keywords

  • biomaterials
  • biopharmaceuticals
  • plant cell culture
  • stable expression
  • transient expression

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Published Papers (1 paper)

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Review

16 pages, 1846 KiB  
Review
Transient Expression of Glycosylated SARS-CoV-2 Antigens in Nicotiana benthamiana
by Valentina Ruocco and Richard Strasser
Plants 2022, 11(8), 1093; https://doi.org/10.3390/plants11081093 - 18 Apr 2022
Cited by 13 | Viewed by 4111
Abstract
The current COVID-19 pandemic very dramatically shows that the world lacks preparedness for novel viral diseases. In addition to newly emerging viruses, many known pathogenic viruses such as influenza are constantly evolving, leading to frequent outbreaks with severe diseases and deaths. Hence, infectious [...] Read more.
The current COVID-19 pandemic very dramatically shows that the world lacks preparedness for novel viral diseases. In addition to newly emerging viruses, many known pathogenic viruses such as influenza are constantly evolving, leading to frequent outbreaks with severe diseases and deaths. Hence, infectious viruses are a recurrent burden to our daily life, and powerful strategies to stop the spread of human pathogens and disease progression are of utmost importance. Transient plant-based protein expression is a technology that allows fast and highly flexible manufacturing of recombinant viral proteins and, thus, can contribute to infectious disease detection and prevention. This review highlights recent progress in the transient production of viral glycoproteins in N. benthamiana with a focus on SARS-CoV-2-derived viral antigens. Full article
(This article belongs to the Special Issue Plant-Based Expression System)
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