Submit to Toxins Review for Toxins Propose a Special Issue

Journal Menu

Journal Browser

► Journal Browser

Exposure and Risk Assessment for Mycotoxins

Print Special Issue Flyer
Special Issue Editors
Special Issue Information
Keywords
Benefits of Publishing in a Special Issue
Published Papers

A special issue of Toxins (ISSN 2072-6651). This special issue belongs to the section "Mycotoxins".

Deadline for manuscript submissions: closed (31 December 2016) | Viewed by 94526

Share This Special Issue

Special Issue Editor

Dr. Carlo Brera

E-Mail Website
Guest Editor

Freelancer, Rome, Italy
Interests: risk/exposure assessment of mycotoxins; biomonitoring studies; monitoring plans on mycotoxins
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In accordance with the concept of “One Health” and “Exposome”, the protection of public health is becoming more and more important.

In this context, risk assessment, as a science-led process for establishing the likelihood of adverse effects to human health and the environment from exposure to risk sources, is facing new challenges, derived from emerging and re-emerging risks, as in the case of mycotoxins, which represent a real burden, worldwide, for both food security and food safety.

In the frame of risk assessment, exposure assessment represents a relevant issue in quantifying risks. In order to refer to reliable outputs, uncertainties, and variability, such as the restricted number of observations, the lack of consumption data and/or the use of improper consumption and occurrence databases, and other sources of error leading to the wrong conclusions for at-risk groups, such as infants, toddlers, children and adolescents, pregnant women, vegetarians, and immunodeficient subjects, have to be considered carefully.

In performing risk and exposure assessment studies, multiple approaches can be followed, such as deterministic vs. probabilistic (modeling), monitoring and/or surveillance programs, Total Diet Studies (TDS), and to continue the development of, and updating of, bio-monitoring studies. The use of biomarkers is proving to be very helpful in the assessment of dietary intakes and occupational exposure. All these issues need to be stressed and highlighted in an even more comprehensive way in order to attempt to associate the intake of mycotoxins with specific pathologies, emphasizing the ones occurring in developed countries where concomitant confounding factors are more present.

The focus of this Special Issue of Toxins will be on epidemiological evidence related to mycotoxin intake, the consideration of modified forms of mycotoxins in exposure assessment and risk characterization, the occupational risk related to mycotoxin-contaminated dust inhalation, the exposure of vulnerable population groups, and the outputs from TDS.

Dr. Carlo Brera
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a double-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Toxins is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

(re)emerging risks
modified mycotoxins
risk assessment, total diet studies
biomonitoring studies
occupational exposure
exposure of vulnerable groups
modeling approaches
food intake
monitoring/surveillance plans

Benefits of Publishing in a Special Issue

Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue polices can be found here.

Published Papers (14 papers)

Download All Papers

Order results

Result details

Show export options Show export options

Select all

Export citation of selected articles as:

Research

Jump to: Review

307 KiB

Open AccessArticle

Study on the Association among Mycotoxins and other Variables in Children with Autism

by Barbara De Santis, Maria Elisabetta Raggi, Giorgio Moretti, Francesco Facchiano, Alessandra Mezzelani, Laura Villa, Arianna Bonfanti, Alessandra Campioni, Stefania Rossi, Serena Camposeo, Sabina Soricelli, Gabriele Moracci, Francesca Debegnach, Emanuela Gregori, Francesca Ciceri, Luciano Milanesi, Anna Marabotti and Carlo Brera

Toxins 2017, 9(7), 203; https://doi.org/10.3390/toxins9070203 - 29 Jun 2017

Cited by 47 | Viewed by 8403

Abstract

Environmental factors and genetic susceptibility are implicated in the increased risk of autism spectrum disorder (ASD). Mycotoxins are agricultural contaminants of fungal origin that represent real risk factors for human health and especially for children. Thus, the main hypothesis of this work is that the deterioration of the clinical manifestation of autism in children may result from the exposure to mycotoxins through the consumption of contaminated food. Within a cross-sectional study, a group of autistic children (n = 172) and a group of controls (n = 61) (siblings and non-parental) were recruited in North and South Italy. All children had blood and urine samples taken, for testing some mycotoxins by a LC–MS/MS validated method. Blood samples were also tested for assessing specific IgG against food and fungal antigens and cytokines. The analyses outputs highlighted statistically significant differences comparing mycotoxins levels between (i) children groups both in urine (deoxynivalenol and de-epoxydeoxynivalenol, p = 0.0141 and p = 0.0259, respectively) and serum (aflatoxin M1, ochratoxin A and fumonisin B1, p = 0.0072, p = 0.0141 and p = 0.0061, respectively); (ii) a group of selected fungal IgGs, and IgGs against wheat and gluten and (iii) cytokines. These results suggest the need for a deeper examination of the role that mycotoxins may have on the etiology of ASD. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

257 KiB

Open AccessArticle

Survey on Urinary Levels of Aflatoxins in Professionally Exposed Workers

by Fulvio Ferri, Carlo Brera, Barbara De Santis, Giorgio Fedrizzi, Tiziana Bacci, Lorena Bedogni, Sauro Capanni, Giorgia Collini, Enrica Crespi, Francesca Debegnach, Patrizia Ferdenzi, Angelo Gargano, Daniela Gattei, Ferdinando Luberto, Ines Magnani, Massimo Giuseppe Magnani, Pamela Mancuso, Simonetta Menotta, Stefania Mozzanica, Milva Olmi, Giuseppe Ombrini, Orietta Sala, Sabina Soricelli, Massimo Vicentini and Paolo Giorgi Rossi Show full author list Hide full author list

Toxins 2017, 9(4), 117; https://doi.org/10.3390/toxins9040117 - 24 Mar 2017

Cited by 31 | Viewed by 4677

Abstract

Feed mill workers may handle or process maize contaminated with aflatoxins (AFs). This condition may lead to an unacceptable intake of toxins deriving from occupational exposure. This study assessed the serological and urinary levels of AFs in workers exposed to potentially contaminated dusts in two mills. From March to April 2014, blood and urine samples were collected, on Monday and Friday morning of the same working week from 29 exposed workers and 30 non-exposed controls. AFs (M₁, G₂, G₁, B₁, B₂) and aflatoxicol (AFOH) A were analyzed. Each subject filled in a questionnaire to evaluate potential food-borne exposures to mycotoxins. AFs contamination in environmental dust was measured in both plants. No serum sample was found to be positive. Seventy four percent of urine samples (73.7%) revealed AFM₁ presence. AFM₁ mean concentration was 0.035 and 0.027 ng/mL in exposed and non-exposed workers, respectively (p = 0.432); the concentration was slightly higher in Friday’s than in Monday’s samples, in exposed workers, 0.040 versus (vs.) 0.031 and non-exposed controls (0.030 vs. 0.024, p = 0.437). Environmental AFs contamination ranged from 7.2 to 125.4 µg/kg. The findings of this study reveal the presence of higher AFs concentration in exposed workers than in non-exposed controls, although these differences are to be considered consistent with random fluctuations. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

3997 KiB

Open AccessArticle

Nitrate Increased Cucumber Tolerance to Fusarium Wilt by Regulating Fungal Toxin Production and Distribution

by Jinyan Zhou, Min Wang, Yuming Sun, Zechen Gu, Ruirui Wang, Asanjan Saydin, Qirong Shen and Shiwei Guo

Toxins 2017, 9(3), 100; https://doi.org/10.3390/toxins9030100 - 11 Mar 2017

Cited by 43 | Viewed by 9022

Abstract

Cucumber Fusarium wilt, induced by Fusarium oxysporum f. sp. cucumerinum (FOC), causes severe losses in cucumber yield and quality. Nitrogen (N), as the most important mineral nutrient for plants, plays a critical role in plant–pathogen interactions. Hydroponic assays were conducted to investigate the effects of different N forms (NH₄⁺ vs. NO₃^‒) and supply levels (low, 1 mM; high, 5 mM) on cucumber Fusarium wilt. The NO₃^‒-fed cucumber plants were more tolerant to Fusarium wilt compared with NH₄⁺-fed plants, and accompanied by lower leaf temperature after FOC infection. The disease index decreased as the NO₃^‒ supply increased but increased with the NH₄⁺ level supplied. Although the FOC grew better under high NO₃⁻ in vitro, FOC colonization and fusaric acid (FA) production decreased in cucumber plants under high NO₃⁻ supply, associated with lower leaf membrane injury. There was a positive correlation between the FA content and the FOC number or relative membrane injury. After the exogenous application of FA, less FA accumulated in the leaves under NO₃⁻ feeding, accompanied with a lower leaf membrane injury. In conclusion, higher NO₃⁻ supply protected cucumber plants against Fusarium wilt by suppressing FOC colonization and FA production in plants, and increasing the plant tolerance to FA. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Figure 1

936 KiB

Open AccessArticle

Individual and Combined Cytotoxic Effects of Co‐Occurring Deoxynivalenol Family Mycotoxins on Human Gastric Epithelial Cells

by Yunxia Yang, Song Yu, Yanglan Tan, Na Liu and Aibo Wu

Toxins 2017, 9(3), 96; https://doi.org/10.3390/toxins9030096 - 9 Mar 2017

Cited by 54 | Viewed by 5589

Abstract

Mycotoxin contamination is a significant health concern for human beings, but health risk assessments are usually based on one single mycotoxin, which might neglect the additive or competitive interactions between co‐occurring mycotoxins [...] Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Figure 1

276 KiB

Open AccessArticle

Multi‐Mycotoxin Analysis in Durum Wheat Pasta by Liquid Chromatography Coupled to Quadrupole Orbitrap Mass Spectrometry

by Josefa Tolosa, Giulia Graziani, Anna Gaspari, Donato Chianese, Emilia Ferrer, Jordi Mañes and Alberto Ritieni

Toxins 2017, 9(2), 59; https://doi.org/10.3390/toxins9020059 - 9 Feb 2017

Cited by 45 | Viewed by 5605

Abstract

A simple and rapid multi‐mycotoxin method for the determination of 17 mycotoxins simultaneously is described in the present survey on durum and soft wheat pasta samples. Mycotoxins included in the study were those mainly reported in cereal samples: ochratoxin‐A (OTA), aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON), 3‐and 15‐acetyl‐deoxynivalenol (3‐AcDON and 15‐AcDON), nivalenol (NIV), neosolaniol (NEO), fusarenon‐X, (FUS‐X), T‐2 toxin (T‐2) and HT‐2 toxin (HT‐2), fumonisin B1 and B2 (FB1 and FB2), and four emerging mycotoxins: three enniatins (ENA, ENA1, and ENB), and beauvericin (BEA). Twenty‐nine samples were analyzed to provide an overview on mycotoxin presence: 27 samples of durum wheat pasta, and two samples of baby food. Analytical results concluded that trichothecenes showed the highest incidence, mainly DON, NIV, and HT‐2 toxin, followed by ZON and ENB, while NEO, FUS‐X, OTA, AFB1, and FUM were not detected in any sample. The highest contents corresponded to ENB and ranged from 91.15μg/kg to 710.90 μg/kg.
Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

1446 KiB

Open AccessArticle

A Survey of Aflatoxin-Producing Aspergillus sp. from Peanut Field Soils in Four Agroecological Zones of China

by Chushu Zhang, Jonathan Nimal Selvaraj, Qingli Yang and Yang Liu

Toxins 2017, 9(1), 40; https://doi.org/10.3390/toxins9010040 - 20 Jan 2017

Cited by 37 | Viewed by 5840

Abstract

Peanut pods are easily infected by aflatoxin-producing Aspergillus sp.ecies from field soil. To assess the aflatoxin-producing Aspergillus sp. in different peanut field soils, 344 aflatoxin-producing Aspergillus strains were isolated from 600 soil samples of four agroecological zones in China (the Southeast coastal zone (SEC), the Yangtze River zone (YZR), the Yellow River zone (YR) and the Northeast zone (NE)). Nearly 94.2% (324/344) of strains were A. flavus and 5.8% (20/344) of strains were A. parasiticus. YZR had the highest population density of Aspergillus sp. and positive rate of aflatoxin production in isolated strains (1039.3 cfu·g⁻¹, 80.7%), the second was SEC (191.5 cfu·g⁻¹, 48.7%), the third was YR (26.5 cfu·g⁻¹, 22.7%), and the last was NE (2.4 cfu·g⁻¹, 6.6%). The highest risk of AFB₁ contamination on peanut was in YZR which had the largest number of AFB₁ producing isolates in 1g soil, followed by SEC and YR, and the lowest was NE. The potential risk of AFB₁ contamination in peanuts can increase with increasing population density and a positive rate of aflatoxin-producing Aspergillus sp. in field soils, suggesting that reducing aflatoxigenic Aspergillus sp. in field soils could prevent AFB₁ contamination in peanuts. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Figure 1

4742 KiB

Open AccessArticle

Gestational Zearalenone Exposure Causes Reproductive and Developmental Toxicity in Pregnant Rats and Female Offspring

by Xin Gao, Lvhui Sun, Niya Zhang, Chong Li, Jiacai Zhang, Zhuohui Xiao and Desheng Qi

Toxins 2017, 9(1), 21; https://doi.org/10.3390/toxins9010021 - 5 Jan 2017

Cited by 60 | Viewed by 7843

Abstract

Zearalenone (ZEN) is an oestrogenic mycotoxin commonly found in food and feed products and can affect reproduction and development in both humans and animals. This study aimed to determine the toxic effects of ZEN on maternal SD rats and the F1 female offspring. Sixty-four pregnant rats were divided into 4 groups and exposed to feed contaminated with ZEN (0, 5, 10, and 20 mg/kg feed) on gestational days (GDs) 0–21. Compared with the controls, the groups exposed to 10 and 20 mg/kg ZEN showed significantly decreased feed intake and body weight of pregnant rats and/or female offspring. Meanwhile, 20 mg/kg ZEN significantly decreased the birth weight and viability of F1 newborn rats. Moreover, 10 and 20 mg/kg ZEN diets increased follicle-stimulating hormone concentrations but decreased oestradiol in both maternal and F1 adult rats. In the F1 generation, ZEN caused no pathological changes in ovaries and uterus in weaned rats, but significant follicular atresia and a thinning uterine layer were found in F1 female adult rats in the 20 mg/kg ZEN group. These impairments concurred with the inhibited mRNA and protein levels of oestrogen receptor-alpha (Esr1) and 3β-hydroxysteroid dehydrogenase (HSD) in the adult uterus and/or ovaries. Furthermore, 10 and/or 20 mg/kg ZEN exposure significantly reduced Esr1, gonadotropin-releasing hormone receptor (GnRHr), and ATP binding cassette transporters b1 and c1 (ABCb1 and ABCc1) in the placenta and foetal and weaned F1 brains, and also produced a dose-dependent increase in 3β-HSD in the placenta. Additionally, 20 mg/kg ZEN significantly upregulated ABCc5 expression in the placenta and ovaries of weaned rats. These results suggested that prenatal ZEN exposure in rats affected maternal and foetal development and may lead to long-term reproductive impairment in F1 adult females. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Figure 1

973 KiB

Open AccessArticle

Are Treated Celiac Patients at Risk for Mycotoxins? An Italian Case-Study

by Martina Cirlini, Teresa Mazzeo, Leda Roncoroni, Vincenza Lombardo, Luca Elli, Maria T. Bardella, Carlo Agostoni, Luisa Doneda, Furio Brighenti, Chiara Dall’Asta and Nicoletta Pellegrini

Toxins 2017, 9(1), 11; https://doi.org/10.3390/toxins9010011 - 28 Dec 2016

Cited by 8 | Viewed by 4192

Abstract

Urinary biomarkers of mycotoxin exposure were evaluated in a group of celiac patients (n = 55) and in a control group of healthy subjects (n = 50) following their habitual diet. Deoxynivalenol (DON), zearalenone (ZEN), and fumonisin B1 (FB1) were monitored in 105 urinary samples collected from the two groups. Dietary habits were also recorded through compilation of a seven-day weighed dietary diary. Biomarkers of mycotoxin exposure were detected in 21 celiac patients and in 15 control subjects, corresponding to about 34% of total participants. In particular, ZEN was the most detected mycotoxin among all the studied subjects with a total of 19 positive cases. Results did not show a statistically significant difference in mycotoxin exposure between the two groups, and the presence of specific mycotoxins was not related to the intake of any particular food category. Our findings suggest little urgency of specific regulation for gluten free products, although the prevalence of exposure observed in free-living diets of both celiac and healthy subjects underlines the need of a constant surveillance on mycotoxins occurrence at large. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Figure 1

1055 KiB

Open AccessArticle

Frequent Occupational Exposure to Fusarium Mycotoxins of Workers in the Swiss Grain Industry

by Hélène Niculita-Hirzel, Gregoire Hantier, Ferdinand Storti, Gregory Plateel and Thierry Roger

Toxins 2016, 8(12), 370; https://doi.org/10.3390/toxins8120370 - 12 Dec 2016

Cited by 30 | Viewed by 6743

Abstract

Type B trichotecens such as deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), nivalenol (NIV) and zearalenone (ZEN) are mycotoxins contaminating wheat and wheat dust. Mycotoxins are toxic upon ingestion and considered potentially toxic when inhaled. Whereas dietary exposure to mycotoxins is controlled in food, data on occupational exposure by inhalation by grain workers are scarce. The objectives of this study were to determine the incidence of DON, 3-ADON, 15-ADON, NIV and ZEN in aerosols generated during grain harvesting and unloading and the risk of exposure of grain workers. Aerosols were collected during the threshing of 78 winter wheat fields and grain unloading of 59 grain lots in six grain terminals in the Vaud region (Switzerland). The samples represented the diversity of the winter wheat cultivar and of the farming system (88 treated with fungicides, 46 untreated). Using a HPLC MS/MS method developed to quantify mycotoxins in aerosols, we report that the mycotoxin content of aerosols was not affected by the wheat cultivars or farming system, but that the incidence of the mycotoxins differed between activities. While wheat harvesting generated on average 28, 20 and 1 ng·m⁻³ of DON, NIV and ZEN, respectively, grain unloading generated 53, 46 and 4 ng·m⁻³. Personal sampling revealed that working in a cab was an efficient protective measure. However, it was not sufficient to avoid chronic exposure to multiple mycotoxins. The most exposed activity was the cleaning, exposing workers to DON, NIV and ZEN at concentrations as high as 65, 59 and 3 ng·m⁻³. These data provide valuable information for future studies of mycotoxin toxicity at relevant concentrations on respiratory health. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Graphical abstract

267 KiB

Open AccessArticle

Exposure Assessment of Infants to Aflatoxin M₁ through Consumption of Breast Milk and Infant Powdered Milk in Brazil

by Angélica T. Ishikawa, Cássia R. Takabayashi-Yamashita, Elisabete Y. S. Ono, Artur K. Bagatin, Fabiana F. Rigobello, Osamu Kawamura, Elisa Y. Hirooka and Eiko N. Itano

Toxins 2016, 8(9), 246; https://doi.org/10.3390/toxins8090246 - 31 Aug 2016

Cited by 60 | Viewed by 7693

Abstract

Aflatoxin M₁ (AFM₁) is an important biomarker that can be used to evaluate aflatoxin exposure in both humans and animals. The aim of this study was to evaluate the exposure degree of infants to AFM₁ through consumption of breast milk and infant powdered milk in Brazil. For this purpose, the estimated daily intake (EDI) for infants was calculated based on the AFM₁ levels analyzed in 94 breast milk (BM) samples collected in Southern Brazil, and 16 infant powdered milk (IPM) samples commonly commercialized in Brazil. AFM₁ was detected in 5.3% (n = 5) and 43.8% (n = 7) of BM and IPM samples, with mean levels of 0.003 ng/g and 0.011 ng/g, respectively. All the IPM samples showed AFM₁ levels lower than those established by the Brazilian guidelines (5 ng/g), and in most of the samples (81.25%) levels were below the maximum limit tolerated by the European Commission (0.025 ng/g). The EDI of AFM₁ for infants aged zero to 12 months old showed values from 0.018 to 0.069 ng/kg body weight/day for BM, and 0.078 to 0.306 ng/kg body weight/day for IPM. Hazard index (HI) values for BM and IPM were less than one, except for IPM intended for infants up to one month. In conclusion, the exposure of infants to AFM₁ was low, but continuous monitoring of mycotoxin levels is essential to minimize infant health risk. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Graphical abstract

1006 KiB

Open AccessArticle

Occurrence of Fusarium langsethiae and T-2 and HT-2 Toxins in Italian Malting Barley

by Caterina Morcia, Giorgio Tumino, Roberta Ghizzoni, Franz W. Badeck, Veronica M.T. Lattanzio, Michelangelo Pascale and Valeria Terzi

Toxins 2016, 8(8), 247; https://doi.org/10.3390/toxins8080247 - 20 Aug 2016

Cited by 57 | Viewed by 6370

Abstract

T-2 and HT-2 toxins are two of the most toxic members of type-A trichothecenes, produced by a number of Fusarium species. The occurrence of these mycotoxins was studied in barley samples during a survey carried out in the 2011–2014 growing seasons in climatically different regions in Italy. The percentage of samples found positive ranges from 22% to 53%, with values included between 26 and 787 μg/kg. The percentage of samples with a T-2 and HT-2 content above the EU indicative levels for barley of 200 μg/kg ranges from 2% to 19.6% in the 2011–2014 period. The fungal species responsible for the production of these toxins in 100% of positive samples has been identified as Fusarium langsethiae, a well-known producer of T-2 and HT-2 toxins. A positive correlation between the amount of F. langsethiae DNA and of the sum of T-2 and HT-2 toxins was found. This is the first report on the occurrence of F. langsethiae—and of its toxic metabolites T-2 and HT-2—in malting barley grown in Italy. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Graphical abstract

6713 KiB

Open AccessArticle

Hydrolytic Fate of 3/15-Acetyldeoxynivalenol in Humans: Specific Deacetylation by the Small Intestine and Liver Revealed Using in Vitro and ex Vivo Approaches

by El Hassan Ajandouz, Stéphane Berdah, Vincent Moutardier, Thierry Bege, David Jérémie Birnbaum, Josette Perrier, Eric Di Pasquale and Marc Maresca

Toxins 2016, 8(8), 232; https://doi.org/10.3390/toxins8080232 - 28 Jul 2016

Cited by 38 | Viewed by 6181

Abstract

In addition to deoxynivalenol (DON), acetylated derivatives, i.e., 3-acetyl and 15-acetyldexynivalenol (or 3/15ADON), are present in cereals leading to exposure to these mycotoxins. Animal and human studies suggest that 3/15ADON are converted into DON after their ingestion through hydrolysis of the acetyl moiety, the site(s) of such deacetylation being still uncharacterized. We used in vitro and ex vivo approaches to study the deacetylation of 3/15ADON by enzymes and cells/tissues present on their way from the food matrix to the blood in humans. We found that luminal deacetylation by digestive enzymes and bacteria is limited. Using human cells, tissues and S9 fractions, we were able to demonstrate that small intestine and liver possess strong deacetylation capacity compared to colon and kidneys. Interestingly, in most cases, deacetylation was more efficient for 3ADON than 15ADON. Although we initially thought that carboxylesterases (CES) could be responsible for the deacetylation of 3/15ADON, the use of pure human CES1/2 and of CES inhibitor demonstrated that CES are not involved. Taken together, our original model system allowed us to identify the small intestine and the liver as the main site of deacetylation of ingested 3/15ADON in humans. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Graphical abstract

Review

Jump to: Research

236 KiB

Open AccessReview

Deoxynivalenol Exposure in Norway, Risk Assessments for Different Human Age Groups

by Leif Sundheim, Inger Therese Lillegaard, Christiane Kruse Fæste, Anne-Lise Brantsæter, Guro Brodal and Gunnar Sundstøl Eriksen

Toxins 2017, 9(2), 46; https://doi.org/10.3390/toxins9020046 - 4 Feb 2017

Cited by 40 | Viewed by 6086

Abstract

Deoxynivalenol (DON) is the most common mycotoxin in Norwegian cereals, and DON is detected in most samples of crude cereal grain and cereal food commodities such as flour, bran, and oat flakes. The Norwegian Scientific Committee for Food Safety assessed the risk for adverse effects of deoxynivalenol (DON) in different age groups of the domestic population. This review presents the main results from the risk assessment, supplemented with some recently published data. Impairment of the immune system together with reduced feed intake and weight gain are the critical effects of DON in experimental animals on which the current tolerable daily intake was established. Based on food consumption and occurrence data, the mean exposure to DON in years with low and high levels of DON in the flour, respectively, were in the range of or up to two times the Tolerable Daily Intake (TDI) in 1-year-old infants and 2-year-old children. In years with high mean DON concentration, the high (95th-percentile) exposure exceeded the TDI by up to 3.5 times in 1-, 2- , 4-, and 9-year-old children. The assessment concluded that exceeding the TDI in infants and children is of concern. The estimated dietary DON intakes in adolescent and adult populations are in the range of the TDI or below, and are not a health concern. Acute human exposure to DON is not of concern in any age group. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Graphical abstract

574 KiB

Open AccessReview

Worldwide Mycotoxins Exposure in Pig and Poultry Feed Formulations

by Philippe Guerre

Toxins 2016, 8(12), 350; https://doi.org/10.3390/toxins8120350 - 24 Nov 2016

Cited by 66 | Viewed by 8831

Abstract

The purpose of this review is to present information about raw materials that can be used in pig and poultry diets and the factors responsible for variations in their mycotoxin contents. The levels of mycotoxins in pig and poultry feeds are calculated based on mycotoxin contamination levels of the raw materials with different diet formulations, to highlight the important role the stage of production and the raw materials used can have on mycotoxins levels in diets. Our analysis focuses on mycotoxins for which maximum tolerated levels or regulatory guidelines exist, and for which sufficient contamination data are available. Raw materials used in feed formulation vary considerably depending on the species of animal, and the stage of production. Mycotoxins are secondary fungal metabolites whose frequency and levels also vary considerably depending on the raw materials used and on the geographic location where they were produced. Although several reviews of existing data and of the literature on worldwide mycotoxin contamination of food and feed are available, the impact of the different raw materials used on feed formulation has not been widely studied. Full article

(This article belongs to the Special Issue Exposure and Risk Assessment for Mycotoxins)

► Show Figures

Journal Menu

Journal Browser

Exposure and Risk Assessment for Mycotoxins

Share This Special Issue

Special Issue Editor

Special Issue Information

Keywords

Benefits of Publishing in a Special Issue

Published Papers (14 papers)

Research

Review

Further Information

Guidelines

MDPI Initiatives

Follow MDPI