Veterinary Sciences

10 pages, 2676 KiB

Open AccessArticle

The Impact of Short-Term Formalin Fixation on Weight and Ventricular Dimensions in the Hearts of Cats and Small-to-Medium-Sized Dogs

by Izabela Janus-Ziółkowska and Joanna Bubak

Vet. Sci. 2025, 12(1), 74; https://doi.org/10.3390/vetsci12010074 - 20 Jan 2025

Viewed by 700

Abstract

Organ weight and size are important data collected during post-mortem examination not only in neoplastic diseases but also in other conditions, like cardiomyopathies. As post-mortem cardiac examination is challenging, it should be performed by experienced specialists. Nonetheless, the low number of referral centres [...] Read more.

Organ weight and size are important data collected during post-mortem examination not only in neoplastic diseases but also in other conditions, like cardiomyopathies. As post-mortem cardiac examination is challenging, it should be performed by experienced specialists. Nonetheless, the low number of referral centres in veterinary medicine requires the shipment of formalin-fixed specimens to perform detailed post-mortem cardiac examinations. Data regarding other tissues suggest that even short-term formalin fixation can influence the organ’s size and/or weight. Presently, no data are available on the impact of formalin fixation on heart weight and size in dogs and cats. The aim of this study was to evaluate the impact of short-term formalin fixation on cardiac weight and ventricular dimensions in cats and small-to-medium-sized dogs in the context of the usefulness and accuracy of referral examination. The study was conducted on 134 hearts (95 feline and 39 canine) obtained during necropsy. Each heart was dissected, cleaned, dried, and immersed in a 7% formalin solution for over 72 h. Weighing of the whole heart and measuring of the ventricular diameters was performed prior to fixation, after 24, 48, 72, and over 72 h of fixation. The study showed that in cats and small-to-medium-sized dogs, heart weight and ventricular dimensions are not significantly influenced by formalin fixation (p > 0.05). Nonetheless, the process of cleaning and drying the heart affects the organ weight significantly (p < 0.0001). The hearts of cats and small-to-medium-sized dogs can be shipped in formalin fixative for a referral opinion, as the fixation does not affect the obtained measurements. Full article

(This article belongs to the Special Issue Veterinary Forensic Pathology and Forensic Investigations in Veterinary Practice)

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10 pages, 876 KiB

Open AccessCommunication

Application of Minimally Invasive Oral Swab Samples for qPCR-Based Sexing in Neognathae Birds

by Maria-Carmen Turcu, Anamaria Ioana Paștiu, Lucia-Victoria Bel, Anca-Alexandra Doboși and Dana Liana Pusta

Vet. Sci. 2025, 12(1), 73; https://doi.org/10.3390/vetsci12010073 - 20 Jan 2025

Viewed by 697

Abstract

Birds are inherently social creatures that rely on pairing to enhance their well-being. Since many bird species lack obvious physical differences between females and males, sex identification is essential for ensuring their welfare. Additionally, early determination of the sexes of birds is crucial [...] Read more.

Birds are inherently social creatures that rely on pairing to enhance their well-being. Since many bird species lack obvious physical differences between females and males, sex identification is essential for ensuring their welfare. Additionally, early determination of the sexes of birds is crucial for their breeders, especially considering that most companion birds do not display clear sexual characteristics. Molecular genetic sexing has been demonstrated to be the most reliable method for determining the sexes of monomorphic birds. The objective of the present study was to demonstrate rapid, effective, and precise identification of sex in birds through quantitative real-time PCR (qPCR) using samples obtained via a minimally invasive technique (oral swabs). This qPCR method assesses variations in gene copy numbers within conserved Z-specific genes such as CHRNA6, DDX4, VPS13A, LPAR1, and TMEM161B, which are absent from the W chromosome. A total of 34 samples were included in this study from the following 17 bird species: domestic pigeon (Columba livia domestica), domestic chicken (Gallus gallus domesticus), domestic goose (Anser anser f domesticus), domestic duck (Anas platyrhynchos domesticus), Mute swan (Cygnus olor), Budgerigar (Melopsittacus undulatus), Lovebird (Agapornis roseicollis), Cockatiel (Nymphicus hollandicus), Red-rumped parrot (Psephotus haematonotus), Rose-ringed parakeet (Psittacula krameri), African grey parrot (Psittacus erithacus), domestic Canary (Serinus canaria forma domestica), Goldfinch (Carduelis carduelis major), Gouldian Finch (Chloebia gouldiae), Red Siskin (Carduelis cucullata), Australian Zebra Finch (Taeniopygia castanotis), and Common buzzard (Buteo buteo). The results proved that the CHRNA6, DDX4, VPS13A, LPAR1, and TMEM161B genes can reveal the sexes in the Neognath birds tested. Full article

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11 pages, 3238 KiB

Open AccessArticle

Biomechanical Comparison of Self-Compressing Screws and Cortical Screw Inserted with Lag Fashion in Canine Cadaveric Humeral Condylar Fracture Model

by Jun-sik Cho, Jung Moon Kim, Youn-woo Choo, Jooyoung Kim, Sorin Kim and Hwi-yool Kim

Vet. Sci. 2025, 12(1), 72; https://doi.org/10.3390/vetsci12010072 - 20 Jan 2025

Viewed by 645

Abstract

This study compares the compression force of cortical screws used in lag fashion with partially threaded cannulated screws and fully threaded headless cannulated screws as fixation methods for humeral condylar fractures in dogs. Cadavers of eleven dogs weighing an average of 10.99 ± [...] Read more.

This study compares the compression force of cortical screws used in lag fashion with partially threaded cannulated screws and fully threaded headless cannulated screws as fixation methods for humeral condylar fractures in dogs. Cadavers of eleven dogs weighing an average of 10.99 ± 2.51 kg (6.1–14.4 kg) were used. The humeri were subjected to simulated fracture by performing an osteotomy at the trochlea of humerus and classified into three groups: Group 1 applied a 3.0 mm cortical screw applied in a lag fashion, Group 2 applied a 3.0 mm partially threaded cannulated screw, and Group 3 applied a 3.5 mm fully threaded headless cannulated screw. The samples were then placed in a material testing machine, and a compression force was applied vertically to the lateral condyle until failure. There were statistically significant differences in failure load between the groups (p = 0.009). The maximum failure load in Group 3 was significantly higher than in Group 2 (p = 0.014), while there were no statistically significant differences between Group 1 and Group 2) or between Group 1 and Group 3. Partially threaded cannulated screws and fully threaded headless cannulated screws can be alternatives to traditional stabilization methods, offering simpler procedures and additional advantages. Full article

(This article belongs to the Section Veterinary Surgery)

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13 pages, 792 KiB

Open AccessSystematic Review

Cognitive Bias in Adult Zebrafish (Danio rerio): A Systematic Review

by Valentina Gazzano, Asahi Ogi, Francesca Cecchi, Maria Claudia Curadi, Maria Marchese and Angelo Gazzano

Vet. Sci. 2025, 12(1), 71; https://doi.org/10.3390/vetsci12010071 - 20 Jan 2025

Viewed by 606

Abstract

Objective: In recent years, the use of zebrafish (Danio rerio) as laboratory models has significantly increased. Ensuring their welfare is crucial, with the cognitive bias test emerging as a valuable tool to assess their emotional state. This systematic review examines the [...] Read more.

Objective: In recent years, the use of zebrafish (Danio rerio) as laboratory models has significantly increased. Ensuring their welfare is crucial, with the cognitive bias test emerging as a valuable tool to assess their emotional state. This systematic review examines the application of the cognitive bias test in zebrafish research. Data: The review adhered to PRISMA guidelines. Sources: A systematic search was conducted in scientific databases, including PubMed and Scopus, yielding 30 articles. Additionally, one abstract identified through bibliographic screening of selected papers was included. Study Selection: Six studies were selected for analysis, and their quality was assessed using SYRCLE’s risk-of-bias tool. Results: Three experimental models were employed to conduct the cognitive bias test in zebrafish, focusing on the relationship between their affective state and environmental conditions, cognitive abilities, and life-history strategies but none of the studies adequately reported the techniques employed to reduce bias. Discussion: The cognitive bias test proved effective in assessing the emotional state of zebrafish; however, methodological biases may have influenced the results. Conclusion: The cognitive bias test offers valuable insights into how emotional states affect cognitive abilities, responses to environmental changes (e.g., physical enrichment), and stress responses. Further research addressing methodological limitations is necessary to validate these findings and improve data reproducibility. Full article

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20 pages, 3376 KiB

Open AccessSystematic Review

A Systematic Review of Medical Treatments for Benign Prostatic Hyperplasia in Dogs: Evaluating Strategies for Reproductive Function Preservation

by Florin Petrișor Posastiuc, Nicolae Tiberiu Constantin, Guillaume Domain, Ann Van Soom, Alexandru Ilie Diaconescu and Mario Darius Codreanu

Vet. Sci. 2025, 12(1), 70; https://doi.org/10.3390/vetsci12010070 - 19 Jan 2025

Viewed by 619

Abstract

Benign prostatic hyperplasia (BPH) is a common condition in intact male dogs and a leading cause of subfertility. While surgical and chemical castration are effective treatments for BPH, managing this condition in breeding dogs is challenging due to the desire to preserve reproductive [...] Read more.

Benign prostatic hyperplasia (BPH) is a common condition in intact male dogs and a leading cause of subfertility. While surgical and chemical castration are effective treatments for BPH, managing this condition in breeding dogs is challenging due to the desire to preserve reproductive function. This study systematically reviews medical treatments for BPH that maintain breeding potential. Thus, a comprehensive literature search was conducted across four databases (CAB Direct, Scopus, PubMed, and Web of Science) to identify peer-reviewed studies published between 2000 and 2024, following PRISMA guidelines. Specific inclusion and exclusion criteria were applied, and a risk of bias assessment was performed using a modified Cochrane tool. A total of 35 studies were included, evaluating therapies such as finasteride, osaterone acetate, tamoxifen, and others, with outcomes including prostate size, sperm quality, hormonal levels, and tissue morphology. Evidence suggests that osaterone acetate and finasteride are acceptable treatments for BPH in breeding dogs, in spite of a still debatable effect on some sperm characteristics. Therapies like tamoxifen and acyline should be excluded due to their detrimental effects on reproductive function. Further research is required for apparently promising approaches concerning tadalafil, anastrazole, mepartricin, and U. fissa. Full article

(This article belongs to the Special Issue Current Therapy in Theriogenology)

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25 pages, 1617 KiB

Open AccessArticle

‘It’s Already Hard and It’s Nearing Impossible’: A Thematic Analysis of Submissions by Rural Veterinarians to the NSW Parliamentary Inquiry into the Veterinary Workforce Shortage

by Sharon Mary Pepita Thio and Anne Quain

Vet. Sci. 2025, 12(1), 69; https://doi.org/10.3390/vetsci12010069 - 17 Jan 2025

Viewed by 1137

Abstract

There is a global veterinary workforce shortage, particularly in rural and regional areas. In response to this shortage in New South Wales (NSW), the State Parliament launched a Parliamentary Inquiry in 2023. The Inquiry received 205 publicly available written submissions, providing insights into [...] Read more.

There is a global veterinary workforce shortage, particularly in rural and regional areas. In response to this shortage in New South Wales (NSW), the State Parliament launched a Parliamentary Inquiry in 2023. The Inquiry received 205 publicly available written submissions, providing insights into the perceived challenges and barriers for veterinarians working in rural practice, and how the perceived impact of the feminisation of the workforce interacts with this. By analysing a subset of submissions written by veterinarians reporting lived experiences in rural practice (n = 63), we identified eight major themes. These were as follows: rural practices are not financially sustainable; rural veterinarians often have a more challenging and higher workload than their urban counterparts; working in rural practice increases challenges to health and wellbeing; it is difficult to recruit and retain people in rural practice; veterinary students are poorly selected and not well prepared for rural practice; clients have unrealistic expectations of rural veterinarians; rural practice is not compatible with family life; and veterinarians have mixed opinions regarding whether an increase in the proportion of female veterinarians is a key contributing factor in the shortage of veterinarians. These findings may assist in the development of recruitment and retention strategies for rural veterinary practice. Full article

(This article belongs to the Section Veterinary Education, Veterinary Communication and Animal Behavior)

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13 pages, 2395 KiB

Open AccessArticle

Black Soldier Fly (Hermetia illucens) Larvae as a Protein Substitute in Adverse Food Reactions for Canine Dermatitis: Preliminary Results Among Patients

by Nuttawan Srifawattana, Yuthana Phimolsiripol, Pinpanit Boonchuay, Kannika Na-Lampang, Promporn Piboon, Sonthaya Umsumarng and Korakot Nganvongpanit

Vet. Sci. 2025, 12(1), 68; https://doi.org/10.3390/vetsci12010068 - 17 Jan 2025

Viewed by 723

Abstract

Can black soldier fly (BSF) larvae be a protein substitute for managing adverse food reactions (AFRs) in dogs, specifically those with dermatitis? We evaluated BSF larvae’s safety, tolerance, and effectiveness as a novel protein source in dogs with AFRs. Sixteen dogs, including eight [...] Read more.

Can black soldier fly (BSF) larvae be a protein substitute for managing adverse food reactions (AFRs) in dogs, specifically those with dermatitis? We evaluated BSF larvae’s safety, tolerance, and effectiveness as a novel protein source in dogs with AFRs. Sixteen dogs, including eight healthy controls and eight diagnosed with AFRs, were fed a diet containing BSF larvae for four weeks. Skin conditions, including dermatological lesions and Pruritus Visual Analog Scale (PVAS) scores, and gastrointestinal signs, including relevant clinical signs, stool consistency, and hematological and biochemical parameters, were monitored. No significant gastrointestinal side effects were observed. The control and AFRs groups maintained stable body weights, while stool consistency scores did not change significantly. Moreover, skin reaction data obtained before the study were collected through interviews with the owners. The mean PVAS scores in the AFRs group were significantly higher (p < 0.05) during the self-control period (6.0 ± 1.6) when compared with other periods. However, no significant differences (p > 0.05) were observed between the pre-study baseline (2.0 ± 1.1) and scores at 2 weeks (2.3 ± 1.5) and 4 weeks (2.4 ± 1.2) of feeding with BSF-based food, which indicated that BSF larvae did not exacerbate pruritic symptoms. Hematology and blood chemistry remained within normal ranges in all dogs, indicating no adverse effects on overall health. BSF larvae were well tolerated by AFR-affected and control dogs, with no adverse clinical outcomes. The findings suggest that BSF larvae may offer a better, sustainable alternative protein source for managing AFRs in dogs, particularly in those on hypoallergenic diets. Further long-term studies are needed to confirm these results and assess the broader implications of BSF larvae diets on canine health. Full article

(This article belongs to the Section Nutritional and Metabolic Diseases in Veterinary Medicine)

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24 pages, 612 KiB

Open AccessReview

Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review

by Raghavendra B. Narasimha, Singireddy Shreya, Vijay Anand Jayabal, Vikas Yadav, Prasana Kumar Rath, Bidyut Prava Mishra, Sudhakar Kancharla, Prachetha Kolli, Gowtham Mandadapu, Sudarshan Kumar, Ashok Kumar Mohanty and Manoj Kumar Jena

Vet. Sci. 2025, 12(1), 67; https://doi.org/10.3390/vetsci12010067 - 17 Jan 2025

Viewed by 876

Abstract

Stem cells are unique, undifferentiated cells that have the ability to both replicate themselves and develop into specialized cell types. This dual capability makes them valuable in the development of regenerative medicine. Current development in stem cell research has widened their application in [...] Read more.

Stem cells are unique, undifferentiated cells that have the ability to both replicate themselves and develop into specialized cell types. This dual capability makes them valuable in the development of regenerative medicine. Current development in stem cell research has widened their application in cell therapy, drug discovery, reproductive cloning in animals, and cell models for various diseases. Although there are substantial studies revealing the treatment of human degenerative diseases using stem cells, this is yet to be explored in livestock animals. Many diseases in livestock species such as mastitis, laminitis, neuromuscular disorders, autoimmune diseases, and some debilitating diseases are not covered completely by the existing drugs and treatment can be improved by using different types of stem cells like embryonic stem cells, adult stem cells, and induced pluripotent stem cells. This review mainly focuses on the use of stem cells for disease treatment in livestock animals. In addition to the diseases mentioned, the potential of stem cells can be helpful in wound healing, skin disease therapy, and treatment of some genetic disorders. This article explores the potential of stem cells from various sources in the therapy of livestock diseases and also their role in the conservation of endangered species as well as disease model preparation. Moreover, the future perspectives and challenges associated with the application of stem cells in livestock are discussed. Overall, the transformative impact of stem cell research on the livestock sector is comprehensively studied which will help researchers to design future research work on stem cells related to livestock diseases. Full article

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26 pages, 3115 KiB

Open AccessArticle

Efficacy of Chlorogenic Acid in Treating Tripterygium Glycoside-Induced Asthenozoospermia in Rats and Its Possible Mechanisms

by Long Chen, God’spower Bello-Onaghise, Mo Chen, Shunda Li, Yu Zhang, Haoran Wang, Qianwei Qu and Yanhua Li

Vet. Sci. 2025, 12(1), 66; https://doi.org/10.3390/vetsci12010066 - 16 Jan 2025

Viewed by 608

Abstract

Tripterygium glycosides (TGs) are the most common form of traditional Chinese medicine, known as Tripterygium wilfordii Hook F (TWHF) [...] Full article

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19 pages, 2892 KiB

Open AccessArticle

Effects of Production System With or Without Growth-Promoting Technologies on Growth and Blood Expression of (Cyto)Chemokines and Heat Shock and Tight Junction Proteins in Bos taurus and indicus Breeds During Summer Season

by Mark Branine, Ashley K. Schilling-Hazlett, Pedro H. V. Carvalho, Kim R. Stackhouse-Lawson, Edilane C. Martins, Julia T. da Silva, Laura Amundson, Chris Ashworth, Mike Socha and Sami Dridi

Vet. Sci. 2025, 12(1), 65; https://doi.org/10.3390/vetsci12010065 - 16 Jan 2025

Viewed by 668

Abstract

Heat stress (HS) induced by global warming is a real welfare, productivity, and economic burden of cattle production. However, some cattle breeds have superior physiological adaptive traits to others, yet the underlying molecular mechanisms are not fully defined. The present study aimed, therefore, [...] Read more.

Heat stress (HS) induced by global warming is a real welfare, productivity, and economic burden of cattle production. However, some cattle breeds have superior physiological adaptive traits to others, yet the underlying molecular mechanisms are not fully defined. The present study aimed, therefore, to determine the expression profile of stress-related molecular signatures in the blood of thermosensitive Angus (Bos taurus) and thermotolerant Brahman (Bos indicus) cattle breeds managed without (CON) or with growth-promoting technology (TRT) during the summer (April–October, 2023) season in Colorado, US. Body weight (BW) was significantly increased from April to October, and the amplitude was greater for the Angus compared to the Brahman breed. The TRT system slightly increased BW, mainly in the Angus breed. Molecular analyses showed that all tested genes were expressed in beef cattle blood. When comparing production systems, the expression of HSP1A1 was significantly upregulated, and HSP90 was downregulated in CON compared to TRT cattle. The expression of IL6, CCL20, and OCLN was induced by the CON system only in the Angus and not in the Brahman breed. At the breed level, Angus cattle exhibited greater expression of IL10, CCL20, and CLDN1 compared to their Brahman counterparts. There was a significant period by production system as well as period by breed interactions. The expression of HSP1A1 increased in both breeds during October. The expression of IL10, CXCL14, CXCR2, and CLDN1 was affected by the production systems in a period-dependent manner. However, the expression of IL6, CXCL14, CCL5, and CXCR2 was upregulated in Angus cattle in a period-sensitive manner. In summary, HSPs, (chemo)cytokines, and tight junction proteins are expressed in the whole blood of beef cattle, and their expression is regulated in a breed-, period-, and/or production system-dependent manner. This could open new vistas for future research to identify molecular signatures for non-invasive stress monitoring and/or marker-assisted genetic selection for robustness and resilience to HS. Full article

(This article belongs to the Section Veterinary Physiology, Pharmacology, and Toxicology)

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18 pages, 1880 KiB

Open AccessArticle

A One Health Perspective on Multidrug Resistance Amongst Iberian Exotic Pet Owners

by Fábio Cardoso-Freitas, Stéphanie M. Mota, Vanessa Silva, Albert Martinez-Silvestre, Ângela Martins and Patrícia Poeta

Vet. Sci. 2025, 12(1), 64; https://doi.org/10.3390/vetsci12010064 - 16 Jan 2025

Viewed by 856

Abstract

Understanding owners’ realities and perceptions may be crucial to helping veterinarians be more effective in their daily work. Noticing the lack of publications in this area, specifically on exotic pets, prompted us to gather insights into non-traditional companion animal owners. A survey was [...] Read more.

Understanding owners’ realities and perceptions may be crucial to helping veterinarians be more effective in their daily work. Noticing the lack of publications in this area, specifically on exotic pets, prompted us to gather insights into non-traditional companion animal owners. A survey was conducted, resulting in 541 respondents from Portugal and Spain. The results were characterised based on demographics, data on trade and antibiotic and parasiticide use, and the relations between these variables. We found that there is still a considerable part of unregistered animals and that, overall, owners acknowledged antimicrobial resistance and inadequate use of parasiticides. Hopefully, these data will help veterinarians in tackling these problems and enhance owners’ education and compliance. Full article

(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)

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8 pages, 2069 KiB

Open AccessCase Report

Transient Third-Degree Atrioventricular Block in a Dog with Addisonian Crisis

by Paula Maria Pașca, Gheorghe Solcan, Andrei Blageanu, Caroline Maria Lăcătuș, Petru Cosmin Peștean, Constantin Adrian Stancu and Andrei Radu Baisan

Vet. Sci. 2025, 12(1), 63; https://doi.org/10.3390/vetsci12010063 - 16 Jan 2025

Viewed by 585

Abstract

A 3-year-old spayed male mixed-breed Labrador presented to the Emergency and Critical Care Unit with lethargy, loss of appetite, vomiting, a recent history of presyncopal episodes, and severe exercise intolerance. On admission, the patient had bradycardia, low blood pressure, and mild abdominal pain. [...] Read more.

A 3-year-old spayed male mixed-breed Labrador presented to the Emergency and Critical Care Unit with lethargy, loss of appetite, vomiting, a recent history of presyncopal episodes, and severe exercise intolerance. On admission, the patient had bradycardia, low blood pressure, and mild abdominal pain. Serum biochemistry information revealed severe hyperkalemia, hyponatremia, hypoglycemia, and mildly increased liver and kidney parameters. Upon taking these findings into consideration, hypoadrenocorticism was suspected, and a basal cortisol level was determined; it was below 1.0 µg/dL. Upon examination with ultrasound, we observed that the size of the adrenal glands was considerably reduced. Due to the bradycardia, a complete cardiologic examination consisting of 5 min of six-lead electrocardiography and echocardiography (ECG) was performed. Six-lead ECG revealed a complete atrio-ventricular block with an atrial rate of 140 bpm and a ventricular rate of 60 bpm. The PQ interval was variable, without any evidence of atrio-ventricular conduction. Treatment for hyperkalemia was initiated immediately after admission, followed by dexamethasone sodium phosphate administration, which led to an improvement in the patient’s general condition, and the normalization of the ECG readings, after 24 h of therapy. Afterward, prednisolone (0.5 mg/kg/24 h p.o.) and desoxycorticosterone pivalate (2.2 mg/kg s.c.) were administered. This case shows that a third-degree atrioventricular block might be a reversible problem in dogs with hypoadrecorticism that can be managed with specific treatment for this disorder, without requiring pacemaker implantation. Full article

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12 pages, 2941 KiB

Open AccessArticle

Effects of Chlorogenic Acid on In Vitro Maturation and Vitrification Cryopreservation of Sheep Oocytes

by Hong Tao, Yukun Zhao, Qiang Zhang, Xu Li, Guangdong Hu, Yanping Wang and Weibin Zeng

Vet. Sci. 2025, 12(1), 62; https://doi.org/10.3390/vetsci12010062 - 16 Jan 2025

Viewed by 545

Abstract

Chlorogenic acid (CGA) has strong antioxidant properties. In order to improve the low maturation rate and poor vitrification freezing effect of sheep oocytes caused by oxidative stress. In this study, oocytes from 200 2–3-year-old Kazakh sheep were collected, and different concentrations of CGA [...] Read more.

Chlorogenic acid (CGA) has strong antioxidant properties. In order to improve the low maturation rate and poor vitrification freezing effect of sheep oocytes caused by oxidative stress. In this study, oocytes from 200 2–3-year-old Kazakh sheep were collected, and different concentrations of CGA were added to the maturation medium and vitrification freezing solution to study the effects of CGA on the maturation rate, cleavage rate, blastocyst rate, reactive oxygen species (ROS) and glutathione (GSH) levels, mitochondrial membrane potential, and the expression levels of oxidation and apoptosis-related genes in sheep oocytes. The results showed that adding 40 μmol/L CGA to the oocyte in vitro maturation solution significantly increased the maturation rate of oocytes, adding 50 μmol/L CGA to the vitrification cryopreservative solution significantly increased the cleavage and blastocyst rates of mature oocytes activated by parthenogenetic activation after freezing. During in vitro maturation and vitrification freezing in sheep oocytes, CGA significantly reduced the level of ROS and the expression of apoptosis-related genes (Caspase-3 and Bax/Bcl-2), and significantly increased the level of glutathione (GSH), mitochondrial membrane potential, and the expression of antioxidant and anti-apoptosis-related genes (SOD-2 and GPX-3). In addition, CGA significantly increased the expression of the anti-apoptotic gene (AKT) and anti-stress gene (FOXO) during vitrification freezing of sheep oocytes. In conclusion, 40 μmol/L CGA improves the maturation rate of sheep oocytes, and 50 μmol/L CGA improves the quality of parthenogenetic activation embryos after vitrification freezing of mature oocytes in sheep. These results provide a basis for the production of sheep in vitro embryos and the establishment of a germplasm resource bank. Full article

(This article belongs to the Section Veterinary Reproduction and Obstetrics)

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14 pages, 3042 KiB

Open AccessArticle

Isolation and Genomic Characterization of a Novel Porcine Reproductive and Respiratory Syndrome Virus 1 from Severely Diseased Piglets in China in 2024

by Shuai Yang, Meng Cui, Chen Li, Ming Qiu, Xiaoyang Zhu, Yanhan Lin, Yifan Meng, Yuejia Qiu, Wenhao Qi, Hong Lin, Wanglong Zheng, Jianzhong Zhu, Kewei Fan and Nanhua Chen

Vet. Sci. 2025, 12(1), 61; https://doi.org/10.3390/vetsci12010061 - 15 Jan 2025

Viewed by 558

Abstract

Since the first isolation of the porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) BJEU06-1 strain from a Beijing pig farm in 2006, more and more PRRSV-1 isolates have been identified in China. In this study, we performed the routine detection of PRRSV-1 [...] Read more.

Since the first isolation of the porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) BJEU06-1 strain from a Beijing pig farm in 2006, more and more PRRSV-1 isolates have been identified in China. In this study, we performed the routine detection of PRRSV-1 using 1521 clinical samples collected in 12 provinces/cities from February 2022 to May 2024. Only three lung samples from severely diseased piglets collected in January 2024 were detected as PRRSV-1-positive (0.197%, 3/1521). A PRRSV-1 strain (AHEU2024-2671) was successfully isolated in primary alveolar macrophages (PAMs) but not in Marc-145 cells. Genome sequencing showed that the AHEU2024-2671 isolate shared the highest genome similarity (90.67%) with the SC2020-1 isolate but only 84.01% similarity with the predominant BJEU06-1 strain. Noticeably, the AHEU2024-2671-like isolates not only contained deletions in nsp2 and the GP3-GP4 overlap region, but also contained a unique 6 nt deletion between nsp12 and the ORF2 gene. Furthermore, a genome-based phylogenetic tree supported that the AHEU2024-2671-like isolates form a novel subgroup within subtype 1. Overall, this study not only supported the idea that PRRSV-1 is rapidly evolving in Chinese swine herds, but also pulled the alarm that novel PRRSV-1 isolates with potentially increased pathogenicity might already exist in China, although they are still rarely detected among Chinese pigs. Full article

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15 pages, 1407 KiB

Open AccessArticle

Efficacy of Fat Supplements with Different Unsaturated/Saturated FA Ratios Undergoing First Postpartum Ovulation in Lactating Anovulatory Goats

by Caroline P. Silva, César C. L. Fernandes, Juliana P. M. Alves, Camila M. Cavalcanti, Felipe B. B. Oliveira, Alfredo J. H. Conde, Diana Celia S. N. Pinheiro, Darcio I. A. Teixeira, Anibal C. Rego and Davide Rondina

Vet. Sci. 2025, 12(1), 60; https://doi.org/10.3390/vetsci12010060 - 15 Jan 2025

Viewed by 610

Abstract

We investigated whether microalgae or linseed supply during the early postpartum period affects ovarian restimulation and supports the first postpartum ovulation in lactating anovulatory goats. Thirty-eight An-glo-Nubian-crossbred adult goats were allocated into three groups, one with a control diet (n = 12), [...] Read more.

We investigated whether microalgae or linseed supply during the early postpartum period affects ovarian restimulation and supports the first postpartum ovulation in lactating anovulatory goats. Thirty-eight An-glo-Nubian-crossbred adult goats were allocated into three groups, one with a control diet (n = 12), fed a total mixed ration (TMR) comprising chopped elephant grass and concentrate; an algal diet (n = 13), fed TMR + green microalgae (1% dry matter); and a linseed diet (n = 13), TMR + linseed (12% dry matter). Supplements were furnished from the second to fifth week (time of weaning). Goats were estrus synchronized on day 40 by insertion of an intravaginal CIDR device for 5 days, after which 0.075 mg PGF2α was applied to induce ovulation, and estrus was monitored for 72 h. From the 5th–15th day of ovulation induction, the corpus luteum (CL) area and progesterone rate were monitored. The algal and linseed groups showed lower feed intake (p < 0.001) and higher (p < 0.001) triglyceride levels/follicle numbers, respectively. After estrus induction, no differences were observed in estrus response; however, the linseed group showed more and larger growing follicles (p = 0.016 and p < 0.01), a higher ovulation rate (p < 0.05), a larger CL area (p < 0.05), and higher progesterone levels (p < 0.001). Linseed after delivery stimulates follicular growth before and after ovulation induction, favoring better CL quality during the first ovulation. Full article

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11 pages, 1012 KiB

Open AccessArticle

Effect of Time and Temperature on the Detection of PRRSV RNA and Endogenous Internal Sample Control in Porcine Tongue Fluids

by Isadora F. Machado, Onyekachukwu H. Osemeke, Kent Doolittle, Cesar A. A. Moura, Lucina Galina Pantoja, Giovani Trevisan, Phillip Gauger and Daniel C. L. Linhares

Vet. Sci. 2025, 12(1), 59; https://doi.org/10.3390/vetsci12010059 - 15 Jan 2025

Viewed by 611

Abstract

Population-based sampling has improved pathogen monitoring in the US swine industry by increasing sensitivity while reducing costs. Postmortem tongue fluids (TF) have emerged as a practical option for monitoring porcine reproductive and respiratory syndrome virus (PRRSV) in breeding herds, but limited data exist [...] Read more.

Population-based sampling has improved pathogen monitoring in the US swine industry by increasing sensitivity while reducing costs. Postmortem tongue fluids (TF) have emerged as a practical option for monitoring porcine reproductive and respiratory syndrome virus (PRRSV) in breeding herds, but limited data exist on optimal storage conditions. This study evaluated PRRSV RNA detection via RT-qPCR in TF samples under various storage times, temperatures, and viral loads. Additionally, the porcine endogenous reference gene (internal sample control, ISC) was assessed for monitoring sample quality. Three studies were conducted: Study 1 compared fresh TF samples to freeze–thaw TF samples, with significantly lower cycle threshold (Ct) values in fresh samples. Studies 2 and 3 evaluated PRRSV RNA stability across six time points (0 to 336 h) and four temperatures (−20 °C, 4 °C, 22 °C, and 34 °C). At 4 °C, Ct value variation was minimal (≤1.1) over 14 days (336 h), with no significant changes within 48 h. In contrast, storage at 22 °C and 34 °C resulted in increased Ct values. The ISC RNA showed similar stability patterns, maintaining quality at 4 °C but degrading at higher temperatures. In conclusion, storing TF at ≤4 °C for up to 14 days for PRRSV and ISC RNA detection is the best scenario, ensuring optimal diagnostic quality. Full article

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12 pages, 2211 KiB

Open AccessArticle

An Improved Method for Extracting Rat Cerebrospinal Fluid with Repeatable Large-Scale Collection

by Limei Wang, Wei Yang, Yanhong Ran, Hui Song, Xinxin Yan and Jianmin Guo

Vet. Sci. 2025, 12(1), 58; https://doi.org/10.3390/vetsci12010058 - 15 Jan 2025

Viewed by 536

Abstract

The aim of this study was to explore an improved method for extracting rat cerebrospinal fluid (CSF), observing the impact on animal health under conditions of large-scale CSF collection and evaluating the feasibility of repeated collections. A total of 20 rats were anesthetized [...] Read more.

The aim of this study was to explore an improved method for extracting rat cerebrospinal fluid (CSF), observing the impact on animal health under conditions of large-scale CSF collection and evaluating the feasibility of repeated collections. A total of 20 rats were anesthetized and fixed in a stereotactic frame. A 26G scalp needle, combined with a 1 mL syringe, was used to puncture the atlanto-occipital membrane and collect approximately 170 μL of CSF. CSF was collected twice within 14 days. During the study, animals were monitored daily for food intake, body weight, and hematological parameters, and at the end of the study, histopathological examination was performed. The health of the animals remained good, and repeated CSF collections were feasible. The success rate of the procedure was 100%, with blood contamination in the CSF decreasing from 70% in the first collection to 35% in the second. This technique is convenient, accurate, and suitable for widespread applications. Full article

(This article belongs to the Section Veterinary Surgery)

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15 pages, 2580 KiB

Open AccessArticle

Biological Characteristics and Whole-Genome Analysis of a Porcine E. coli Phage

by Shenghui Wan, Nana Li, Sajid Habib, Pei Zheng, Yanfang Li, Yan Liang and Yonggang Qu

Vet. Sci. 2025, 12(1), 57; https://doi.org/10.3390/vetsci12010057 - 14 Jan 2025

Viewed by 602

Abstract

(1) Background: In recent years, the increasing emergence of multidrug-resistant pathogens in pig farms has begun to pose a severe threat to animal welfare and, by extension, public health. In this study, we aimed to explore the biological characteristics and genomic features of [...] Read more.

(1) Background: In recent years, the increasing emergence of multidrug-resistant pathogens in pig farms has begun to pose a severe threat to animal welfare and, by extension, public health. In this study, we aimed to explore the biological characteristics and genomic features of bacteriophages that are capable of lysing porcine multidrug-resistant E. coli, which was isolated from sewage. In doing so, we provided a reference for phage therapies that can be used to treat multidrug-resistant strains. (2) Method: Using the multidrug-resistant E. coli isolate sq-1 as the host bacterium, bacteriophages were isolated and purified from fecal samples using a double-layer agar plate method. The morphology was observed using a transmission electron microscope, and its host range, optimal multiplicity of infection (MOI), one-step growth curve, thermal stability, acid–base tolerance, and in vitro antibacterial ability were tested. Genomic features were analyzed using whole-genome sequencing. (3) Results: A lytic phage named vB_EcoS_Psq-1 (abbreviated as Psq-1) was successfully isolated. Electron microscopy revealed that Psq-1 belongs to the family of long-tailed phages, possessing clear and transparent plaques of approximately 1 mm in diameter. Psq-1 only lyses the host bacterium and does not affect other E. coli strains or other species of bacteria. The optimal MOI for phage Psq-1 was 0.1, with a latent period of 25 min, an exponential growth period of 25 min, and a lysis yield of 44.21 PFU/cell. Its activity remains stable at temperatures between 40 °C and 60 °C and from pH 4.0 to pH 13.0. Psq-1 exhibited a significant inhibitory effect on E. coli in liquid culture medium. The nucleic acid type of phage Psq-1 was dsDNA, with a total genome length of 44,183 bp and a GC content of 52.16%. No known resistance, lysogenic, or virulence-related genes were detected. The whole genome contains 55 open reading frames (ORFs). (4) Conclusions: This study isolated a bacteriophage that is capable of lysing multidrug-resistant E. coli. Characterized by a narrow E. coli lysis range, a long latent period, limited lytic ability, and stable biological properties, this bacteriophage can serve as a reference isolate for E. coli phages and can provide biological materials and data to support research on bacteriophages that are effective against multidrug-resistant porcine E. coli. Full article

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11 pages, 9028 KiB

Open AccessCase Report

Equine Herpesvirus-1 Induced Respiratory Disease in Dezhou Donkey Foals: Case Study from China, 2024

by Lian Ruan, Liangliang Li, Rongze Yang, Anrong You, Muhammad Zahoor Khan, Yue Yu, Li Chen, Yubao Li, Guiqin Liu, Changfa Wang and Tongtong Wang

Vet. Sci. 2025, 12(1), 56; https://doi.org/10.3390/vetsci12010056 - 14 Jan 2025

Viewed by 641

Abstract

Equine herpesvirus-1 (EHV-1) is a significant pathogen that causes substantial economic losses in the equine industry worldwide, which leads to severe respiratory diseases and abortions in horses. However, reports of EHV-1 infection in donkeys are limited, particularly in China. This case study reported [...] Read more.

Equine herpesvirus-1 (EHV-1) is a significant pathogen that causes substantial economic losses in the equine industry worldwide, which leads to severe respiratory diseases and abortions in horses. However, reports of EHV-1 infection in donkeys are limited, particularly in China. This case study reported an EHV-1-induced respiratory disease in Dezhou donkey foals in Shandong Province, China, in July 2024. Three one-month-old foals exhibited high fever, nasal discharge, and respiratory distress, with a 100% mortality rate. The causative agent, strain LC126, was isolated from a one-month-old donkey foal exhibiting severe respiratory disease. Phylogenetic analysis of the EHV-1 isolate LC126 showed close similarity to EHV-1. Overall, our study revealed that EHV-1 can cause respiratory distress as well as death in donkeys. The study underscores the emerging threat of EHV-1 in donkeys and highlights the need for veterinarians and breeders to give proper attention to the potential threat of EHV-1 outbreaks. Full article

(This article belongs to the Special Issue The Progress of Equine Medical Research in China and Beyond)

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9 pages, 1772 KiB

Open AccessBrief Report

IFN-γ/TNF-α Synergism Induces Pro-Inflammatory Cytokine and Chemokine Production by In Vitro Canine Keratinocytes

by Kyungsook Jung, Ji-Yeong Ku, Je-Seong Kwon, Gayeon Won, Hakyoung Yoon, Sang-Ik Oh, Mi Hye Kim, Chongchan Kim and Ji-Seon Yoon

Vet. Sci. 2025, 12(1), 55; https://doi.org/10.3390/vetsci12010055 - 14 Jan 2025

Viewed by 906

Abstract

Activated keratinocytes play a crucial role in skin inflammation through the production of multiple inflammatory mediators; however, little is known about cytokine secretion by activated keratinocytes in dogs. This study aimed to investigate the effects of the Th1 and Th2 types of cytokines [...] Read more.

Activated keratinocytes play a crucial role in skin inflammation through the production of multiple inflammatory mediators; however, little is known about cytokine secretion by activated keratinocytes in dogs. This study aimed to investigate the effects of the Th1 and Th2 types of cytokines on the production of keratinocyte-derived inflammatory mediators. Canine progenitor epidermal keratinocytes (CPEKs) were incubated with canine recombinant IL-4, IL-13, an IL4/IL13 mixture, IFN-γ, TNF-α, or an IFN-γ/TNF-α mixture for 24 h following 100% confluency. Culture supernatants were analyzed for cytokine concentration, including chemokine ligand (CXCL) 8, IL-10, IL-6, IL-1ß, IL-12, and chemokine ligand 2 (CCL2) by enzyme-linked immunosorbent assay. CPEKs incubated with the IFN-γ/TNF-α mixture showed significantly increased IL-6 concentration. In addition, significantly increased concentrations of CXCL8 were detected in CPEKs incubated with TNF-α and with the IFN-γ/TNF-α mixture. CCL2 concentrations increased in cells incubated with IFN-γ, TNF-α, and the IFN-γ/TNF-α mixture. The IFN-γ/TNF-α mixture synergistically enhanced CCL2 production. Dose-dependent elevations were also observed in IL-6 in response to the IFN-γ/TNF-α mixture, and in CCL2 in response to IFN-γ, TNF-α, and the IFN-γ/TNF-α mixture. These findings indicate that IFN-γ and TNF-α synergistically increase pro-inflammatory cytokines and chemokines secreted by canine keratinocytes. This in vitro culture system could be useful to investigate cytokine-mediated crosstalk between keratinocytes and immune cells and new therapeutic strategies for keratinocyte-mediated inflammatory skin diseases. Full article

(This article belongs to the Section Veterinary Biomedical Sciences)

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13 pages, 2459 KiB

Open AccessArticle

Establishment of Pathogen-Free Rhipicephalus bursa Colonies Under Laboratory Conditions for the Vector Competence Studies

by Mehmet Can Ulucesme, Sezayi Ozubek and Munir Aktas

Vet. Sci. 2025, 12(1), 54; https://doi.org/10.3390/vetsci12010054 - 13 Jan 2025

Viewed by 954

Abstract

Rhipicephalus bursa, the primary vector of Babesia ovis, is also considered to transmit Theileria, Babesia, and Anaplasma spp. These claims are based on pathogen detections rather than experimental validation. To confirm vector competence, sterile ticks must acquire pathogens from [...] Read more.

Rhipicephalus bursa, the primary vector of Babesia ovis, is also considered to transmit Theileria, Babesia, and Anaplasma spp. These claims are based on pathogen detections rather than experimental validation. To confirm vector competence, sterile ticks must acquire pathogens from infected hosts and transmit them to other hosts. The basic step is establishing a pathogen-free tick colony. In this study, engorged R. bursa females were collected from 12 infested livestock and allowed to lay eggs. The carcasses and larvae were screened for tick-borne pathogens using nPCR. The 0.150 g pathogen-free F1 larvae were fed on New Zealand rabbits, resulting in 592 engorged nymphs that molted into F1 adults. Eighty F1 adults were fed on pathogen-free splenectomized sheep, producing the next larval generation (F2). This protocol was repeated to produce F3 larvae. At the end of all developmental stages, ticks were screened via nPCR and found to be negative for tick-borne pathogens. The sheep were monitored for 63 days with no clinical signs or positive nPCR results, confirming F3 larvae as pathogen-free and suitable for vector competence studies. The R. bursa life cycle was completed in 72–153 days, providing a reliable model for vector competence research and offering valuable insights into its biological parameters. Full article

(This article belongs to the Topic Ticks and Tick-Borne Pathogens)

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17 pages, 2313 KiB

Open AccessArticle

Pathophysiology of Penetrating Captive Bolt Stunning in Horned and Polled Sheep and Factors Determining Incomplete Concussion

by Troy John Gibson, Anne Lisa Ridler, Georgina Limon, Christopher Lamb, Alun Williams and Neville George Gregory

Vet. Sci. 2025, 12(1), 53; https://doi.org/10.3390/vetsci12010053 - 13 Jan 2025

Viewed by 594

Abstract

Penetrating captive bolt (PCB) is widely used for stunning and on-farm dispatch of livestock, yet its efficacy can vary, with the potential for animal welfare compromise. This study investigated the pathophysiology of PCB-induced trauma in horned and polled sheep (Ovis aries), [...] Read more.

Penetrating captive bolt (PCB) is widely used for stunning and on-farm dispatch of livestock, yet its efficacy can vary, with the potential for animal welfare compromise. This study investigated the pathophysiology of PCB-induced trauma in horned and polled sheep (Ovis aries), focusing on factors contributing to incomplete concussion. Thirty-seven (n = 18 horned Scottish blackface and n = 19 polled North Country mule) mature ewes (aged 4–10 years) were shot with PCB with varying cartridge power and PCB modifications, followed by clinical assessment and post-mortem analysis using magnetic resonance imagining (MRI) and gross pathology. The results indicated that damage to the reticular activating system, bolt velocity and penetration depth are crucial for inducing irreversible unconsciousness, with depths less than 37 mm often resulting in incomplete concussion. MRI provided detailed insights into brain injuries, aligning well with gross pathological findings. This study highlights the importance of precise bolt placement and appropriate PCB configurations in ensuring humane outcomes, with MRI proving to be a valuable tool for assessing brain trauma in stunned animals. These findings enhance the understanding of effective stunning techniques and support improved welfare practices in livestock management. Full article

(This article belongs to the Section Veterinary Education, Veterinary Communication and Animal Behavior)

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11 pages, 2412 KiB

Open AccessArticle

Enhanced Porcine Reproductive and Respiratory Syndrome Virus Replication in Nsp4- or Nsp2-Overexpressed Marc-145 Cell Lines

by Zhengqin Ye, Zhenbang Zhu, Liangzheng Yu, Zhendong Zhang and Xiangdong Li

Vet. Sci. 2025, 12(1), 52; https://doi.org/10.3390/vetsci12010052 - 13 Jan 2025

Viewed by 546

Abstract

Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry. The killed PRRSV vaccine has been reported to be safe and could elicit humoral responses. The killed PRRSV vaccine with a high viral antigen load combined with robust adjuvants [...] Read more.

Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry. The killed PRRSV vaccine has been reported to be safe and could elicit humoral responses. The killed PRRSV vaccine with a high viral antigen load combined with robust adjuvants could provide good protection against the infection. However, the high virus titer of PRRSV on the successive production cell lines is the prerequisite for this strategy. In this study, we explored PRRSV production in two recombinant Marc-145 cell lines expressing Nsp2 or Nsp4 through a lentivirus system. The results demonstrated that either Nsp2 or Nsp4 expressing Marc-145 cells did not affect cell morphology and growth kinetics but significantly enhanced PRRSV replication. Overall, our exploration may enable the production of high-yield PRRSV and offer a potential tool for developing safer and more effective PRRSV vaccines. Full article

(This article belongs to the Section Veterinary Microbiology, Parasitology and Immunology)

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14 pages, 4085 KiB

Open AccessArticle

Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two Paenibacillus spp. Isolates from a Gray Wolf (Canis lupus) Gastrointestinal Tract

by Jessika L. Bryant, Jennifer McCabe, C. Cristoph Klews, MiCayla Johnson, Ariel N. Atchley, Thomas W. Cousins, Maya Barnard-Davidson, Kristina M. Smith, Mark R. Ackermann, Michael Netherland, Jr., Nur A. Hasan, Peter A. Jordan, Evan S. Forsythe, Patrick N. Ball and Bruce S. Seal

Vet. Sci. 2025, 12(1), 51; https://doi.org/10.3390/vetsci12010051 - 13 Jan 2025

Viewed by 730

Abstract

Inflammatory bowel disease (IBD) is increasing among mammals around the world, and domestic dogs are no exception. There is no approved cure for canine IBD with limited treatment options. Novel probiotic bacteria discovery from free-ranging animals for the treatment of IBD in domestic [...] Read more.

Inflammatory bowel disease (IBD) is increasing among mammals around the world, and domestic dogs are no exception. There is no approved cure for canine IBD with limited treatment options. Novel probiotic bacteria discovery from free-ranging animals for the treatment of IBD in domestic pets can likely yield promising probiotic candidates. Consequently, the overall aim was to isolate bacteria from free-ranging animals that could potentially be utilized as novel probiotics. Two bacteria identified as unique Paenibacillus spp. strains by small ribosomal RNA (16S) gene sequencing were isolated from the gastrointestinal tract of a North American Gray Wolf (Canis lupus). The bacteria were typed as Gram-variable, and both were catalase/oxidase positive as well as sensitive to commonly used antibiotics. The bacteria digested complex carbohydrates and lipids by standard assays. The isolated bacteria also inhibited the growth of Staphylococcus aureus and Micrococcus luteus. The whole genome sequence (WGS) length of bacterial isolate ClWae17B was 6,939,193 bp, while ClWae19 was 7,032,512 bp, both similar in size to other Paenibacillus spp. The genomes of both bacteria encoded enzymes involved with the metabolism of complex starches and lipids, such as lyases and pectinases, along with encoding antimicrobials such as lanthipeptides, lasso peptides, and cyclic-lactone-autoinducers. No pernicious virulence genes were identified in the WGS of either bacterial isolate. Phylogenetically, the most closely related bacteria based on 16S gene sequences and WGS were P. taichungensis for ClWae17B and P. amylolyticus for ClWae19. WGS analyses and phenotypic assays supported the hypothesis that the isolates described constitute two novel candidate probiotic bacteria for potential use in dogs. Full article

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16 pages, 1832 KiB

Open AccessArticle

In Vitro Evaluation of Ruminal Digestibility, Fermentation Characteristics, and Bacterial Diversity of Kenaf Crop at Various Cutting Heights

by Mengwei Li, Faiz-ul Hassan, Qian Lin, Muhammad Adeel Arshad, Muhammad Uzair Akhtar, Lijuan Peng, Chengjian Yang, Xin Liang and Jiaxiang Huang

Vet. Sci. 2025, 12(1), 50; https://doi.org/10.3390/vetsci12010050 - 12 Jan 2025

Viewed by 686

Abstract

The current study investigated the in vitro degradability, in vitro gas production, methane (CH₄) production, and ruminal bacterial community of kenaf plants cut at different heights (130, 160, 190, 220, and 250 cm). These samples were subjected to an in vitro [...] Read more.

The current study investigated the in vitro degradability, in vitro gas production, methane (CH₄) production, and ruminal bacterial community of kenaf plants cut at different heights (130, 160, 190, 220, and 250 cm). These samples were subjected to an in vitro batch culture system using buffalo rumen fluid to measure gas and CH₄ production at 3, 6, 9, 12, 24, 36, 48, and 72 h of incubation. Results reveal that crude protein (CP) concentration was the highest at the 220 cm height compared with the other heights. With the increase in height, gas and CH₄ production decreased. However, the CH₄ production at 190 cm was higher compared with the other plant heights. Dry matter degradation was higher at 190 cm and 220 cm, while ammonia-N and microbial CP were higher at the 220 cm height compared with the other heights. However, neutral detergent fiber degradation was the highest at the 130 cm height. Total volatile fatty acids, acetic acid, acetic acid/propane ratio, and pH value did not differ among the treatments, except for propionic acid, which was higher at the 130 cm and 160 cm heights. Overall, harvesting kenaf at plant heights of up to 220 cm was better in terms of its promising nutritional quality, improved dry matter degradation, and microbial CP contents. Full article

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10 pages, 5037 KiB

Open AccessArticle

Computed Tomography Evaluation of Morphological Types of Femoral Trochlear Dysplasia in Small-Breed Dogs—A Retrospective Study

by Radka S. Garnoeva

Vet. Sci. 2025, 12(1), 49; https://doi.org/10.3390/vetsci12010049 - 12 Jan 2025

Viewed by 961

Abstract

Abnormal trochlear morphology is one of the most important factors for patellar luxation occurrence in dogs, yet no studies have investigated its prevalence in the general population. This retrospective computed tomography study was designed to evaluate the trochlear groove morphology in four small [...] Read more.

Abnormal trochlear morphology is one of the most important factors for patellar luxation occurrence in dogs, yet no studies have investigated its prevalence in the general population. This retrospective computed tomography study was designed to evaluate the trochlear groove morphology in four small dog breeds and the prevalence of trochlear dysplasia types according to Déjour’s classification depending on the breed, sex, and medial patellar luxation (MPL) presence and grade. A total of 174 joints (68 healthy, 96 grade II MPL, and 10 grade III MPL) from Mini-Pinschers, Yorkshire Terriers, Pomeranians, and Chihuahuas were included in the study. The morphological type of trochlear dysplasia (TD) was evaluated on axial scans and 3D reconstruction images according to the four-type classification of Déjour, sulcus angle, trochlear depth, and lateral/medial inclination angles. Of all 174 joints, 140 had trochlear dysplasia—all joints with MPL (n = 106) and 50% of healthy joints (n = 34). The classification of Déjour for trochlear dysplasia types (A, B, C, and D) corresponds to the morphology of the femoral trochlea in the studied small breeds of dogs. The results demonstrated three types of trochlear dysplasia according to Déjour: most commonly, type A, followed by type C, and most infrequently, type D. The Déjour type B was an incidental finding. The large proportion of clinically healthy joints with TD (50%) emphasises the significance of early trochlear morphology evaluation for the orthopaedical health of dogs from susceptible breeds, especially in female breeders. Full article

(This article belongs to the Special Issue Medical Imaging in Veterinary Musculoskeletal Diagnosis)

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12 pages, 1463 KiB

Open AccessCommunication

An Enigmatic Wild Passerine Mortality Event in the Eastern United States

by Sabrina S. Greening, Julie C. Ellis, Nicole L. Lewis, David B. Needle, Cristina M. Tato, Susan Knowles, Valerie Shearn-Bochsler, Jaimie L. Miller, Daniel A Grear, Jeffrey M. Lorch, David S. Blehert, Caitlin Burrell, Lisa A. Murphy, Erica A. Miller, C. Brandon Ogbunugafor, Andrea J. Ayala, W. Kelley Thomas, Joseph L. Sevigny, Lawrence M. Gordon, Tessa Baillargeon, Lusajo Mwakibete, Megan Kirchgessner, Christine L. Casey, Ethan Barton, Michael J. Yabsley, Eman Anis, Roderick B. Gagne, Patrice Klein, Cindy Driscoll, Chelsea A. Sykes, Robert H. Poppenga and Nicole M. Nemeth Show full author list Hide full author list

Vet. Sci. 2025, 12(1), 48; https://doi.org/10.3390/vetsci12010048 - 11 Jan 2025

Viewed by 1184

Abstract

The ability to rapidly respond to wildlife health events is essential. However, such events are often unpredictable, especially with anthropogenic disturbances and climate-related environmental changes driving unforeseen threats. Many events also are short-lived and go undocumented, making it difficult to draw on lessons [...] Read more.

The ability to rapidly respond to wildlife health events is essential. However, such events are often unpredictable, especially with anthropogenic disturbances and climate-related environmental changes driving unforeseen threats. Many events also are short-lived and go undocumented, making it difficult to draw on lessons learned from past investigations. We report on the response to a mortality event observed predominantly in wild passerines in the eastern United States. The event began in May 2021 when wildlife rehabilitators and private citizens reported large numbers of sick and dead juvenile birds, mostly presenting as single cases with neurologic signs and/or ocular and periocular lesions. Early efforts by rehabilitators, veterinarians, state and federal wildlife agencies, and universities helped gather public reports and fuel rapid responses by government agencies. Collective efforts included live bird and carcass collections; submission to diagnostic laboratories and evaluation; information sharing; and coordinated messaging to stakeholders and interested parties. Extensive diagnostic evaluations failed to identify a causative pathogen or other etiology, although congruent results across laboratories have helped drive further investigation into alternative causes, such as nutritional deficiencies. This report highlights the strengths of a multi-agency, interdisciplinary investigation while exposing the need for an operational framework with approaches and resources dedicated to wildlife health. Full article

(This article belongs to the Special Issue Wildlife Health and Disease in Conservation)

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7 pages, 717 KiB

Open AccessBrief Report

Diagnostic Limitations of Applying a Human Portable Blood Glucose Meter in the Detection of Hypoglycemia in Pregnant Ewes

by José Lucas Xavier Lopes, Raquel Fraga e Silva Raimondo, Luiza Rodegheri Jacondino, Beatriz Riet Correa, Clara Satsuki Mori and Álan Gomes Pöppl

Vet. Sci. 2025, 12(1), 47; https://doi.org/10.3390/vetsci12010047 - 11 Jan 2025

Viewed by 648

Abstract

Pregnant ewes are susceptible to hypoglycemia and ketosis; therefore, monitoring glycemic status is extremely important. Portable blood glucose meters (PBGMs) can assist in quickly and conveniently identifying glycemic disturbances in this species, provided that they meet the criteria of analytical accuracy. This study [...] Read more.

Pregnant ewes are susceptible to hypoglycemia and ketosis; therefore, monitoring glycemic status is extremely important. Portable blood glucose meters (PBGMs) can assist in quickly and conveniently identifying glycemic disturbances in this species, provided that they meet the criteria of analytical accuracy. This study evaluated the performance of a human PBGM (Accu-Chek Performa^®, Roche Diagnostics, Basel, Switzerland) in the glycemic evaluation of 34 pregnant ewes at days 90 and 120 of pregnancy in comparison with the results of glycemia determination by a reference method (RM). The device showed a high positive correlation (r = 0.71, 95%CI = 0.57–0.82, p < 0.0001) with the RM; however, 96.6% of the PBGM results (58.5 ± 9.82 mg/dL) were higher (p < 0.0001) than those obtained in the laboratory (48.6 ± 9.31 mg/dL). The PBGM tested was considered analytically inaccurate according to ISO 15197:2013, which states that when glucose levels are below 100 mg/dL, 95% of the measurements should deviate by no more than 15 mg/dL from the RM value, and 1/3 of the PBGM results were above this limit. Hypoglycemia (<50 mg/dL) was documented in 60.29% of samples tested on with the RM, but only 17.64% of results were below 50 mg/dL using the PBGM. Due to these limitations, Accu-Check Performa^® results should be interpreted cautiously in pregnant sheep suspected of hypoglycemia. Full article

(This article belongs to the Special Issue Farm Animal Health and Welfare Disorders: Recent Trends and Strategies of Prevention and Treatment)

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16 pages, 2073 KiB

Open AccessArticle

Characterization, Quantification, and Molecular Identification of Co-Infection of Canine Parvovirus (CPV-2) Variants in Dogs Affected by Gastroenteritis in Ecuador During 2022–2023

by Anthony Loor-Giler, Silvana Santander-Parra, Sara Castillo-Reyes, Martin Campos, Renán Mena-Pérez, Santiago Prado-Chiriboga and Luis Nuñez

Vet. Sci. 2025, 12(1), 46; https://doi.org/10.3390/vetsci12010046 - 11 Jan 2025

Viewed by 1131

Abstract

Canine parvovirus (CPV-2) is a highly contagious virus in canines, and it is mostly spread by touching infected feces. Dogs of all ages can catch it, but puppies are more likely to suffer from it. Severe signs include vomiting, diarrhea with blood, feeling [...] Read more.

Canine parvovirus (CPV-2) is a highly contagious virus in canines, and it is mostly spread by touching infected feces. Dogs of all ages can catch it, but puppies are more likely to suffer from it. Severe signs include vomiting, diarrhea with blood, feeling tired, and not drinking enough water. There are three different types of the original CPV-2 that have been found so far, which are CPV-2a, 2b, and 2c. The genome of CPV-2 is about 5.2 kb long and has two open reading frames (ORFs), namely the VP region and the NS region. Based on changes in amino acids at position 426, the VP2 protein distinguishes the gene apart in the VP region. Using a molecular method, this study contemplated the presence of CPV-2 and its variants in dogs that had gastroenteritis, as well as other infections. There were 511 samples tested, and 401 (78.47%) of them were positive for CPV-2. Of these, 144 (25.91%) were positive for the original genotype, 258 (64.34%) for genotype 2a, 343 (85.54%) for genotype 2b, and 167 (41.65%) for genotype 2c. Using the multiplex qPCR for genotyping, CPV-2a and CPV-2b were determined as the most frequent co-infections (16.45%). The three genotypes (2a, 2b, and 2c) were found in the samples examined based on the amino acids at position 426 of the VP2 protein, as demonstrated by the VP2 gene sequencing. Furthermore, it was discovered that in certain samples, a genetic modification at position 297 was connected to the virus’s pathogenicity. Full article

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9 pages, 232 KiB

Open AccessArticle

How Does Saccharomyces cerevisiae DSM 34246 (Canobios-BL) var. boulardii Supplementation Impact the Fecal Parameters of Healthy Adult Dogs?

by Nicolò Lonigro, Francesca Perondi, Natascia Bruni, Mauro Bigliati, Annalisa Costale, Elena Pagani, Ilaria Lippi, Alice Melocchi, Lucia Zema, Giorgia Meineri and Elisa Martello

Vet. Sci. 2025, 12(1), 45; https://doi.org/10.3390/vetsci12010045 - 10 Jan 2025

Viewed by 1224

Abstract

The gastrointestinal (GI) tract is populated by a variety of microbes, which were recently demonstrated to play a major role in both human and animal health [...] Full article

(This article belongs to the Special Issue Advanced Therapy in Companion Animals)

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Vet. Sci., Volume 12, Issue 1 (January 2025) – 74 articles

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