Hydrobiology, Volume 3, Issue 4 (December 2024) – 7 articles

The previously available coding region for the spiny dogfish (Squalus acanthias) AQP3-2 gene was amplified from cDNAs using PCR. Agarose gel electrophoresis gave a band of the AQP3-2 coding region, as well as multiple smaller splice variant bands. The main AQP3-2 band and the largest and most fluorescently intense pair of these splice variant bands were cloned and sequenced. Amplifications were performed on a range of tissue cDNAs, but AQP3-2 was only expressed in the kidney and brain. Quantitative PCR amplifications using pre-existing kidney cDNA from an environmental salinity acclimation experiment showed that the abundance of mRNA from both the main AQP3-2 transcript and the largest splice variant (Splice Variant 1) was lower in 120% seawater (SW) acclimated fish, although only the values for Splice Variant 1 were statistically significant. A custom-made affinity-purified rabbit polyclonal AQP3-2 antibody was produced, and this gave four bands of around the correct sizes (which were 27 and 32 kDa) for the complete AQP3-2 and Splice Variant 1 proteins. Two of the bands may have been N-glycosylated forms of these proteins. Other bands were also present on the Western blot. No bands were present when the antibody was pre-blocked by the peptide antigen. In tissue sections of the dogfish kidney, immunohistochemical localization experiments showed that AQP3-2 was expressed in the early distal tubule (EDT) and late distal tubule (LDT) nephron segments. The results suggest that AQP3-2 may be involved in cell volume regulation in the EDT and water and urea absorption in the LDT nephron segment. Full article

S. sphyraena is a widely distributed species with low commercial value and no sufficient scientific knowledge of its biology. In the present study, the age, growth, weight–length relationship, otolith morphometry, and reproduction of the species were investigated in the Eastern Ionian Sea for the first time. The von Bertalanffy growth function parameters were L_∞ = 63.65 cm, k = 0.14 year⁻¹ and t₀ = −2.01 years and Φ′ = 2.75. The negative allometric growth in weight was found. Slope b of the weight–length relationship was 2.634. For the otolith moprhometry, the variables radius, length, width, area, perimeter, roundness, circularity, form factor, rectangularity, and ellipticity were examined, which showed that the otolith shape is elongated with an elliptical and rectangular form. Six otolith variables (radius, length, width, area, perimeter, and ellipticity) showed a significant relationship with size. The sex ratio (females/males) was 1:0.74 (no statistically significant difference from 1:1), and the spawning season extended from April to June with peak values of GSI in May for females and April for males. The results of this work improve our knowledge of the species life cycle and provide basic information for species stock identification and fisheries management. Full article

The sea bivalve clam Tridacna crocea inhabiting the shallow sea of tropical and subtropical zones lives with the symbiotic alga zooxanthella in its mantle. Zooxanthellae algae perform photosynthesis and supply nutrients to T. crocea. Recently, the abundance of T. crocea has decreased rapidly due to overfishing in coastal areas in Okinawa, Japan. T. crocea culture systems for mass production will contribute to the conservation of T. crocea and thus marine ecosystems. Environmental control methods for T. crocea culture have not been established because of a lack of knowledge about the appropriate environmental conditions for T. crocea growth. The present study was initiated to obtain basic data for developing environmental control methods for T. crocea land-based aquaculture. The effects of water temperature, dissolved oxygen concentration, and photosynthetic photon flux density (PPFD) on the O₂ exchange rates of the symbiotic system of T. crocea and zooxanthella, which are indicators of photosynthesis and respiration in the system, and the effect of daily integrated PPFD on T. crocea growth were investigated. Basic knowledge was obtained for the development of optimal environmental control technology for T. crocea clam culture. The optimum water temperature and dissolved oxygen concentration for photosynthesis in this symbiotic system were 28 °C, 5–6 mgO₂ L⁻¹ and 500 μmol m⁻² d⁻¹, respectively. The optimum daily integrated PPFD for clam growth was 20 mol m⁻² d⁻¹. Full article

The evaluation of mitochondrial DNA and genetic analysis is helpful for economically significant species. Clarias gariepinus is a critical species in aquaculture. This study investigates the genetic diversity and population differentiation of C. gariepinus from 19 countries using 164 sequences of the mitochondrial DNA’s Cytochrome c oxidase I (COI) gene. The haplotype analysis revealed a total of 17 haplotypes, with a nucleotide diversity (π) of 0.012 and a haplotype diversity (Hd) of 0.87. The results of an AMOVA and fixation index indicated significant genetic variation and structure among the populations. Additionally, neutrality tests and mismatch distribution analysis supported the hypothesis of under-purifying selection in C. gariepinus. The findings suggested that the population did not experience expansion. In conclusion, the genetic analysis highlighted substantial variation among C. gariepinus populations from different locations, providing valuable insights for the global management of this species. Full article

Over the course of industrialization in the 20th century, vast emissions of air pollutants have occurred. The exhaust gasses contain sulfur and nitrogen oxides, which are converted to sulfuric acid and nitric acid in the atmosphere. This causes acid rain to enter aquatic and terrestrial ecosystems, the most serious consequence of which is large-scale forest dieback across Europe and North America. However, through various political measures, the exhaust gasses have been reduced and, thus, acid rain and forest dieback were stopped. Nevertheless, the lingering effects of this pollution are still present today and are reflected in hydrochemistry. More recently, fluctuating precipitation regimes are causing additional stress to ecosystems in Central Europe. Climatic extremes are becoming more pronounced with climate change. Substantial differences between drought years and years with regular precipitation are directly altering the discharge of springs. Now, two overlapping and interacting syndromes of environmental pressures can be studied in these small catchments at a landscape scale: (1) acidification and (2) climate change. In this long-term study, the waters of 102 forest springs, located in two neighboring forest landscapes in north-eastern Bavaria, Germany (Frankenwald and Fichtelgebirge), were investigated over 24 years (1996 to 2020). By linking changes in pH values with changes in precipitation and spring discharge, we found that pH increases with decreasing discharge and decreasing precipitation. This effect was strongest in the Frankenwald compared to the Fichtelgebirge. We hypothesize that this temporal pattern reflects the longer residence time and, in consequence, the increased buffering of acidic interflow in small catchments during periods of drought. However, this should not be misinterpreted as rapid recovery from acidification because this effect fades in times of enhanced precipitation. We recommend that fluctuations in weather regimes be considered when investigating biogeochemical patterns throughout forest landscapes. Full article

Salmonid fishes provide an important indicator of climate change given their reliance on cold water. We evaluated temporal changes in the density of stream-dwelling brook trout (Salvelinus fontinalis) from surveys conducted over a 36-year period (1988–2023) by the Maryland Department of Natural Resources in Eastern North America. Nonparametric trend analyses revealed decreasing densities of adult fish (age 1+) in 19 sites (27%) and increases in 5 sites (7%). In contrast, juvenile fish (age 0) densities decreased in 4 sites (6%) and increased in 10 sites (14%). Declining adult brook trout trends were related to atmospheric warming rates during the study period, and this relationship was stronger than the effects of land use change or non-native brown trout. In contrast, juvenile fish trends generally increased with elevation but were not related to air temperature trends or land use change. Our analysis reveals significant changes in several brook trout populations over recent decades and implicates warming atmospheric conditions in population declines. Our findings also suggest the importance of temperature for adult survival rather than recruitment limitation in brook trout population dynamics. Full article

Freshwater slugs are scarce and belong exclusively to panpulmonate Acochlidimorpha. There is a radiation of eight species of large-sized slugs living benthically in rivers on tropical Indo-Pacific Islands. In the Western Atlantic, only one small interstitial slug, Tantulum elegans Rankin, 1979, is known from the Caribbean island of St. Vincent. We recently discovered a novel species of freshwater slugs in Cuba. Here, we describe Potamohedyle espinosai n. gen. n. sp., which is the first freshwater slug in the region of the Western Atlantic with a benthic lifestyle, in 3D-microanatomical and histological detail using light and scanning electron microscopy. It shows a mix of characters from different freshwater acochlidimorph genera, such as a medium body size, the presence of an osphradial ganglion, a distal gonoduct with a muscular sphincter, a penis with a solid thorn and cuticular comb, and a basal finger with a hollow stylet. Morphological adaptations to a life in freshwater include multiplicated renopericardioducts. The taxonomic character mix justifies the establishment of a novel genus within the herein diagnostically modified freshwater family Tantulidae. A molecular phylogenetic hypothesis of riverine slugs including the first Caribbean representatives suggests that the transition to freshwater occurred once along the stemline of limnic Acochlidiidae, secondarily marine Pseudunelidae and limnic Tantulidae. Full article