Animals

2024

Jump to: 2023, 2022, 2021, 2020

11 pages, 1651 KiB

Open AccessArticle

Assessing Post-Vaccination Seroprevalence and Enhancing Strategies for Lumpy Skin Disease Vaccination in Korean Cattle

by Geun-Ho Kim, Dae-Sung Yoo, Keum-Suk Chu, Eun-Hyo Cho, Seung-Il Wi, Kyung-Ok Song, Do Kyung Ra, Woo H. Kim, Choi-Kyu Park, Dongseob Tark, Yeonsu Oh and Ho-Seong Cho

Animals 2024, 14(22), 3236; https://doi.org/10.3390/ani14223236 - 12 Nov 2024

Viewed by 546

Abstract

Lumpy skin disease (LSD), caused by the LSD virus (LSDV), a dsDNA virus of the genus Capripoxvirus, represents a significant cross-border infectious threat, particularly impacting cattle and water buffaloes through transmission by blood-feeding insects. Traditionally endemic to Southern Africa, LSD has rapidly spread [...] Read more.

Lumpy skin disease (LSD), caused by the LSD virus (LSDV), a dsDNA virus of the genus Capripoxvirus, represents a significant cross-border infectious threat, particularly impacting cattle and water buffaloes through transmission by blood-feeding insects. Traditionally endemic to Southern Africa, LSD has rapidly spread over the past decade through the Middle East to Eastern Europe and China, reaching Korea in October 2023. This outbreak prompted a nationwide vaccination campaign, addressing both the disease’s severe economic impact and its status as a notifiable disease under the World Organisation for Animal Health. This study assesses the seropositivity of the LSD vaccine in cattle across four Korean provinces 2–3 months post-vaccination, aiming to inform improvements in biosecurity and vaccination strategies. Overall, 30.59% of the cattle tested (1196 out of 3910) exhibited positive antibody responses, comparable to international post-vaccination findings. Analysis further revealed differences in the antibody positivity between farm types and management practices. Specifically, farms where vaccines were administered by veterinarians showed no significant difference in antibody positivity between Korean native cattle and dairy cattle, regardless of the presence of restraint facilities. However, on farms where vaccinations were conducted by the owners, dairy cattle demonstrated a higher seropositivity (43.30 ± 33.39%) compared to Korean native cattle (21.97 ± 20.79%) in the absence of restraint facilities. Further comparisons underscored the impact of restraint facilities on vaccination efficacy, with dairy farms generally achieving higher antibody positivity (29.43 ± 30.61%) than farms with Korean native cattle (23.02 ± 23.33%) (p < 0.05), suggesting that consistent vaccine delivery methods enhance immunogenic responses. Contrarily, no significant difference was noted in antibody positivity between large- and small-scale farms, indicating that farm size did not notably impact the effectiveness of the vaccinator. These findings emphasize that while current vaccines are sufficiently inducing immunity, enhancing vaccination strategies, particularly through trained personnel and improved restraint facilities, is crucial. This study’s insights into the impact of vaccination and farm management practices provide valuable guidance for refining LSD control measures in Korea and potentially other affected regions. Full article

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16 pages, 3624 KiB

Open AccessReview

Clostridial Infections in Cattle: A Comprehensive Review with Emphasis on Current Data Gaps in Brazil

by Felipe Masiero Salvarani and Eliel Valentin Vieira

Animals 2024, 14(20), 2919; https://doi.org/10.3390/ani14202919 - 10 Oct 2024

Viewed by 1148

Abstract

Clostridial infections in cattle are a significant concern for Brazilian livestock. These diseases are caused by various species of Clostridium, which are known for their ability to produce potent toxins. Botulism in cattle is a serious and often fatal condition caused by [...] Read more.

Clostridial infections in cattle are a significant concern for Brazilian livestock. These diseases are caused by various species of Clostridium, which are known for their ability to produce potent toxins. Botulism in cattle is a serious and often fatal condition caused by the ingestion of neurotoxins produced by C. botulinum. This bacterium thrives in decomposing organic matter, such as spoiled feed, carcasses, and contaminated water. Tetanus is less common, but it is a serious disease that follows the contamination of wounds with Clostridium tetani spores. It results in muscle stiffness, spasms, and often death due to respiratory failure. Blackleg (C. chauvoei) is a disease that primarily affects young cattle, leading to acute lameness, swelling, and high fever. Malignant edema (C. septicum and others) is characterized by rapid onset of swelling at wound sites, and it can occur after injuries or surgical procedures. Enterotoxemia is triggered by the rapid growth of C. perfringens in the gut following excessive carbohydrate intake. This leads to toxin production that causes sudden death. In conclusion, clostridial bovine infections remain a persistent challenge for Brazilian cattle farmers. With continued focus on vaccination, good management practices, and research, the impact of these diseases can be minimized, safeguarding the livestock industry’s economic viability. Full article

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15 pages, 249 KiB

Open AccessArticle

The Influence of Selected Environmental Factors on the Number of Somatic Cells in Cistern and Alveolar Milk of Polish Holstein-Friesian Cows

by Piotr Guliński and Michał Kacper Kroszka

Animals 2024, 14(15), 2219; https://doi.org/10.3390/ani14152219 - 31 Jul 2024

Viewed by 713

Abstract

The aim of the study was to evaluate the influence of the milking phase on somatic cell count (SCC) in milk obtained from the cisternal and alveolar parts of udders of selected Polish Holstein-Friesian cows. The study also assessed the impact of other [...] Read more.

The aim of the study was to evaluate the influence of the milking phase on somatic cell count (SCC) in milk obtained from the cisternal and alveolar parts of udders of selected Polish Holstein-Friesian cows. The study also assessed the impact of other genetic and environmental factors on SCC variability in cisternal and alveolar milk, including: the individual cow, lactation stage, age of cow, production level, milking speed, fat-to-protein ratio, and milking type. The research included 15 cows of Polish Holstein-Friesian breed at different ages, lactation stages, and with varying daily milk yield. A total of 210 milk observations were conducted, including 105 for 1 min milking and 105 for 8 min milking. The results obtained in the study indicated that milk obtained during two different milking phases exhibited similar SCC levels (F for LOGSCC = 0.79). The average actual SCC in milk produced by 15 cows in 105 observations for 1 min milking was 219,000 cells/mL, while for 8 min milking it was 229,000 cells/mL. The results were inconclusive, suggesting that SCC in cisternal and alveolar milk must be influenced by factors other than the milking phase. The analysis of variance conducted for this purpose provided the basis for stating a highly statistically significant effect of the individual cow (F for LOGSCC = 147.9), lactation stage (F for LOGSCC = 54.64), age of cow (F for LOGSCC = 12.39), daily production level (F for LOGSCC = 34.49), milking speed (F for LOGSCC = 17.56), and fat-to-protein ratio (F for LOGSCC = 22.99) on the variability of characteristics defining SCC in milk. In summary, SCC is characterized by high variability, influenced by a range of environmental and genetic factors such as the individual cow, lactation stage, age of cow, milking speed, and dietary fat-to-protein ratio. The influence of milking phase (1 min or 8 min) and milking type (morning or evening) should be considered inconclusive based on the entire population studied. For half of the cows, SCC in cisternal milk was higher than in alveolar milk, while for the other half, the situation was reversed. Further observations are required to confirm the hypothesis regarding the extent to which cows’ immunological response to bacterial infections is concentrated in the cisternal or alveolar part of the udder under national environmental conditions. Full article

16 pages, 2527 KiB

Open AccessArticle

Detection and Phenotypic Antimicrobial Susceptibility of Salmonella enterica Serotypes in Dairy Cattle Farms in the Po Valley, Northern Italy

by Francesca Parolini, Giordano Ventura, Carlo Rosignoli, Sara Rota Nodari, Mario D’incau, Leonardo Marocchi, Giovanni Santucci, Massimo Boldini and Matteo Gradassi

Animals 2024, 14(14), 2043; https://doi.org/10.3390/ani14142043 - 12 Jul 2024

Cited by 1 | Viewed by 1079

Abstract

The presence of Salmonella spp. in dairy cattle farms poses a major risk to animal health and welfare. This study focused on Salmonella detection in dairy farms located in the Cremona and Mantua provinces (northern Italy) in samples collected and submitted to laboratories [...] Read more.

The presence of Salmonella spp. in dairy cattle farms poses a major risk to animal health and welfare. This study focused on Salmonella detection in dairy farms located in the Cremona and Mantua provinces (northern Italy) in samples collected and submitted to laboratories in 2021–2022. A total of 2710 samples from different sources, including calf carcasses/organs (n = 128), rectal swabs (n = 1937), feces (n = 390), bulk milk (n = 93), and overshoes/swabs (n = 127) for environmental sampling, were analyzed for the presence of Salmonella spp. and were included in the present study. Our results indicate that Salmonella was most commonly firstly identified from calf carcasses and organs (61.67%) and that the serotypes most frequently detected in dairies were S. Dublin (38.33%), S. Typhimurium (23.33%), and S. Typhimurium monophasic variant (14.17%). The most common pathological findings in calf carcasses were enteritis, hepatosplenomegaly, and pneumonia. The antimicrobial resistance pattern analyzed using the MIC assay of 51 Salmonella isolates revealed the presence of multi-resistant strains, which pose a major risk to public and animal health. Full article

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11 pages, 910 KiB

Open AccessArticle

Serological and Community Awareness Study of Lumpy Skin Disease in Different Agro-Ecological Zones of Sidama Regional State, Southern Ethiopia

by Nebyou Moje, Adane Seifu, Gizachew Hailegebreal, Dereje Shegu, Serena Montagnaro and Gianmarco Ferrara

Animals 2024, 14(12), 1782; https://doi.org/10.3390/ani14121782 - 13 Jun 2024

Viewed by 961

Abstract

The lumpy skin disease (LSD) vaccination status and epidemiological distribution remain unknown in some parts of Ethiopia, including the Sidama regional state. In this study, a serological survey of LSD was performed using a specific virus neutralization assay in selected districts of the [...] Read more.

The lumpy skin disease (LSD) vaccination status and epidemiological distribution remain unknown in some parts of Ethiopia, including the Sidama regional state. In this study, a serological survey of LSD was performed using a specific virus neutralization assay in selected districts of the Sidama regional state representing three agroecological zones from September 2021 to June 2022. Moreover, an assessment of community awareness and LSD vaccine-related problems was conducted using a questionnaire. Our results showed an overall animal and herd level seroprevalence of 40.8% (95%CI = 35.8, 45.8) and 81% (95%CI = 77, 85), respectively. High and low seroprevalence were observed in lowland (48%) and highland (28%) areas, although they were not statistically significant. However, risk factors such as management systems and breeds showed substantial differences in their LSD prevalence. The results obtained through the questionnaire showed that a small portion of respondents (29.2%) know about LSD and vaccinate their cattle (23.3%) against this disease. Only 20.8% of the respondents stated that there was LSD occurrence in their vaccinated cattle. In conclusion, both qualitative and quantitative study results showed the need for intervention in terms of community-based awareness creation about LSD clinical signs and vaccination advantages together with the frequently updated information on LSD prevalence. Full article

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11 pages, 10770 KiB

Open AccessCommunication

The Risk Factors of Dewclaw Dermatitis in Beef Cattle in the Amazon Biome

by José Diomedes Barbosa, Janayna Barroso dos Santos, Hanna Gabriela da Silva Oliveira, Tatiane Teles Albernaz Ferreira, José Alcides Sarmento da Silveira, Camila Cordeiro Barbosa, Marilene Farias Brito, Natalia da Silva e Silva Silveira, Carlos Magno Chaves Oliveira, Henrique dos Anjos Bomjardim and Felipe Masiero Salvarani

Animals 2024, 14(9), 1329; https://doi.org/10.3390/ani14091329 - 29 Apr 2024

Viewed by 887

Abstract

Bovine Dewclaw Dermatitis (BDCD) is a hoof disease characterized by inflammation of the second and fifth accessory digits and the skin in this region. This pathology is poorly described in the literature; however, it has recently been observed in beef cattle in the [...] Read more.

Bovine Dewclaw Dermatitis (BDCD) is a hoof disease characterized by inflammation of the second and fifth accessory digits and the skin in this region. This pathology is poorly described in the literature; however, it has recently been observed in beef cattle in the Amazon Biome, Brazil. The objective of this study was to perform a clinical diagnosis and identify the risk factors associated with BDCD onset in cattle in the studied biome. Samples were collected from eight farms with extensive breeding systems located in Xinguara, Rondon do Pará, Curionópolis, and Ipixuna do Pará in the state of Pará, Brazil. A total of 706 Nellore and Nellore crossbred with taurine bovine of both sexes were evaluated, with males aged between 2 and 4 years and a mean weight of 650 kg, and females aged between 2 and 11 years and a mean weight of 400 kg. Distal extremities were inspected during cattle management, and in cases of dewclaw lesions, a specific examination was carried out after proper restraint. Cattle were diagnosed with BDCD on all farms analyzed. Of the 706 cattle inspected, 49 (6.94%) showed BDCD, of which 19 (38.77%) were Nellore and 30 (61.22%) were crossbred. This was the first study to determine BDCD’s occurrence in extensive farming systems in the Amazon region, also showing that pastures with large amounts of stumps and stones, the physical structure of pens, and trauma and injury incidence during animal management are the most important predisposing factors for the onset of BDCD. Full article

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16 pages, 2862 KiB

Open AccessArticle

Phenotypic and Functional Characterization of Bovine Adipose-Derived Mesenchymal Stromal Cells

by Vitali V. Maldonado, Sriya Pokharel, Jeremy G. Powell and Rebekah M. Samsonraj

Animals 2024, 14(9), 1292; https://doi.org/10.3390/ani14091292 - 25 Apr 2024

Viewed by 1261

Abstract

Background: Mesenchymal stem cells (MSCs) are increasingly trialed in cellular therapy applications in humans. They can also be applied to treat a range of diseases in animals, particularly in cattle to combat inflammatory conditions and aging-associated degenerative disorders. We sought to demonstrate the [...] Read more.

Background: Mesenchymal stem cells (MSCs) are increasingly trialed in cellular therapy applications in humans. They can also be applied to treat a range of diseases in animals, particularly in cattle to combat inflammatory conditions and aging-associated degenerative disorders. We sought to demonstrate the feasibility of obtaining MSCs from adipose tissue and characterizing them using established assays. Methods: Bovine adipose MSCs (BvAdMSCs) were isolated using in-house optimized tissue digestion protocols and characterized by performing a colony formation assay, cell growth assessments, cell surface marker analysis by immunocytochemistry and flow cytometry, osteogenic and adipogenic differentiation, and secretion of indoleamine 2,3-dioxygenease (IDO). Results: Our results demonstrate the feasibility of successful MSC isolation and culture expansion from bovine adipose tissues with characteristic features of colony formation, in vitro multilineage differentiation into osteogenic and adipogenic lineages, and cell surface marker expression of CD105, CD73, CD90, CD44, and CD166 with negative expression of CD45. BvAdMSCs secreted significant amounts of IDO with or without interferon–gamma stimulation, indicating ability for immunomodulation. Conclusions: We report a viable approach to obtaining autologous adipose-derived MSCs that can be applied as potential adjuvant cell therapy for tissue repair and regeneration in cattle. Our methodology can be utilized by veterinary cell therapy labs for preparing MSCs for disease management in cattle. Full article

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18 pages, 1223 KiB

Open AccessArticle

Long-Term Survival in 241 Cases of Intussusception in Cattle and Factors Associated with Mortality

by Laurens Chantillon, Mathilde Laetitia Pas, Lieven Vlaminck and Bart Pardon

Animals 2024, 14(5), 676; https://doi.org/10.3390/ani14050676 - 21 Feb 2024

Viewed by 1372

Abstract

Intussusception is a frequent cause of mechanical ileus in cattle. Long-term survival has never been described and risk factors for mortality are scarcely documented. A retrospective cohort study on 241 cattle was conducted to determine survival of intussusception and identify risk factors for [...] Read more.

Intussusception is a frequent cause of mechanical ileus in cattle. Long-term survival has never been described and risk factors for mortality are scarcely documented. A retrospective cohort study on 241 cattle was conducted to determine survival of intussusception and identify risk factors for mortality. Clinical records were matched with the national cattle identification database. Information on possible predictors including clinical examination, ultrasonography, blood-gas analysis and surgery were collected. Survival analysis and decision tree analysis were used. Overall survival was 44.8% until discharge. Of all animals, 39.0% could complete their life cycle and were eligible for slaughter. Male animals and cattle < 226 days old experienced a significantly higher mortality risk (hazard ratio [HR] = 2.1; 95% confidence interval [CI] = 1.4–3.0 and HR = 2.4; 95% CI = 1.7–3.4, respectively). The final model consisted of heart rate (>95 beats per minute) and packed cell volume (<36.5%) with sensitivity and specificity of 60.4% and 49.4%, respectively. A second model consisted of sex (male) and time to referral (>4.5 days) with sensitivity and specificity of 88.0% and 65.6%, respectively. The long-term prognosis for intussusception in cattle appears to be fair. Factors identified in this study may aid in the decision-making process in cases with presumed intussusception to perform the surgery or opt for euthanasia. Full article

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10 pages, 2153 KiB

Open AccessCommunication

Characterization and Spike Gene Analysis of a Candidate Attenuated Live Bovine Coronavirus Vaccine

by Gyu-Nam Park, SeEun Choe, Sok Song, Ki-Sun Kim, Jihye Shin, Byung-Hyun An, Soo Hyun Moon, Bang-Hun Hyun and Dong-Jun An

Animals 2024, 14(3), 389; https://doi.org/10.3390/ani14030389 - 25 Jan 2024

Viewed by 1515

Abstract

The bovine coronavirus (BCoV) KBR-1 strain, obtained from calf diarrhea samples collected in 2017, belongs to group GIIa. To attenuate this strain, it was subcultured continuously (up to 79 times) in HRT-18 cells, followed by 80–120 passages in MDBK cells. The KBR-1-p120 strain [...] Read more.

The bovine coronavirus (BCoV) KBR-1 strain, obtained from calf diarrhea samples collected in 2017, belongs to group GIIa. To attenuate this strain, it was subcultured continuously (up to 79 times) in HRT-18 cells, followed by 80–120 passages in MDBK cells. The KBR-1-p120 strain harvested from MDBK cells at passage 120 harbored 13 amino acid mutations in the spike gene. Additionally, the KBR-1-p120 strain showed a high viral titer and cytopathogenic effects in MDBK cells. Seven-day-old calves (negative for BCoV antigen and antibodies) that did not consume colostrum were orally inoculated with the attenuated candidate strain (KBR-1-p120), or with KBR-1 passaged 10 times (KBR-1-p10) in HRT-18 cells. Calves inoculated with KBR-1-p10 had a low diarrhea score, and BCoV RNA was detected at 3–7 days post-inoculation (DPI). The virus was also present in the duodenum, jejunum, and ileum at autopsy; however, calves inoculated with KBR-1-p120 had low levels of BCoV RNA in feces at 4–6 DPI, and no diarrhea. In addition, an extremely small amount of BCoV RNA was present in the jejunum and ileum at autopsy. The small intestines of calves inoculated with KBR-1-p120 were emulsified and used to infect calves two more times, but pathogenicity was not recovered. Therefore, the KBR-1-p120 strain has potential as a live vaccine candidate. Full article

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27 pages, 2220 KiB

Open AccessReview

The Global Epidemiology of Bovine Leukemia Virus: Current Trends and Future Implications

by Guanxin Lv, Jianfa Wang, Shuai Lian, Hai Wang and Rui Wu

Animals 2024, 14(2), 297; https://doi.org/10.3390/ani14020297 - 18 Jan 2024

Cited by 1 | Viewed by 4904

Abstract

Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leucosis (EBL), which is the most significant neoplastic disease in cattle. Although EBL has been successfully eradicated in most European countries, infections continue to rise in Argentina, Brazil, Canada, Japan, and the [...] Read more.

Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leucosis (EBL), which is the most significant neoplastic disease in cattle. Although EBL has been successfully eradicated in most European countries, infections continue to rise in Argentina, Brazil, Canada, Japan, and the United States. BLV imposes a substantial economic burden on the cattle industry, particularly in dairy farming, as it leads to a decline in animal production performance and increases the risk of disease. Moreover, trade restrictions on diseased animals and products between countries and regions further exacerbate the problem. Recent studies have also identified fragments of BLV nucleic acid in human breast cancer tissues, raising concerns for public health. Due to the absence of an effective vaccine, controlling the disease is challenging. Therefore, it is crucial to accurately detect and diagnose BLV at an early stage to control its spread and minimize economic losses. This review provides a comprehensive examination of BLV, encompassing its genomic structure, epidemiology, modes of transmission, clinical symptoms, detection methods, hazards, and control strategies. The aim is to provide strategic information for future BLV research. Full article

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2023

Jump to: 2024, 2022, 2021, 2020

13 pages, 414 KiB

Open AccessArticle

Leveraging Accelerometer Data for Lameness Detection in Dairy Cows: A Longitudinal Study of Six Farms in Germany

by Anastasia I. Lavrova, Alexander Choucair, Andrea Palmini, Kathrin F. Stock, Martin Kammer, Friederike Querengässer, Marcus G. Doherr, Kerstin E. Müller and Vitaly Belik

Animals 2023, 13(23), 3681; https://doi.org/10.3390/ani13233681 - 28 Nov 2023

Viewed by 1266

Abstract

Lameness in dairy cows poses a significant challenge to improving animal well-being and optimizing economic efficiency in the dairy industry. To address this, employing automated animal surveillance for early lameness detection and prevention through activity sensors proves to be a promising strategy. In [...] Read more.

Lameness in dairy cows poses a significant challenge to improving animal well-being and optimizing economic efficiency in the dairy industry. To address this, employing automated animal surveillance for early lameness detection and prevention through activity sensors proves to be a promising strategy. In this study, we analyzed activity (accelerometer) data and additional cow-individual and farm-related data from a longitudinal study involving 4860 Holstein dairy cows on six farms in Germany during 2015–2016. We designed and investigated various statistical models and chose a logistic regression model with mixed effects capable of detecting lameness with a sensitivity of 77%. Our results demonstrate the potential of automated animal surveillance and hold the promise of significantly improving lameness detection approaches in dairy livestock. Full article

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17 pages, 803 KiB

Open AccessReview

Expression, Regulation, and Function of β-Defensins in the Bovine Mammary Glands: Current Knowledge and Future Perspectives

by Mojtaba Daneshi, Joel S. Caton, Luciano S. Caixeta, Zohre Eftekhari and Alison K. Ward

Animals 2023, 13(21), 3372; https://doi.org/10.3390/ani13213372 - 31 Oct 2023

Cited by 1 | Viewed by 2093

Abstract

β-Defensins are cationic antimicrobial peptides (AMPs) that play an important role in the innate immune defense of bovines. They are constitutively expressed in mammary glands and induced differently in response to pathogens. Their expression is influenced by various factors, including hormones, plant-derived compounds, [...] Read more.

β-Defensins are cationic antimicrobial peptides (AMPs) that play an important role in the innate immune defense of bovines. They are constitutively expressed in mammary glands and induced differently in response to pathogens. Their expression is influenced by various factors, including hormones, plant-derived compounds, and dietary energy imbalance. The toll-like receptors (TLRs)/nuclear factor-kappa B (NF-κB) pathway plays a crucial role in β-defensin induction, while alternative pathways such as mitogen-activated protein kinase (MAPK) and epigenetic regulation also make substantial contributions. β-Defensins exhibit bactericidal activity against a wide range of pathogens, including two major mastitis pathogens, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), primarily through membrane disruption. β-Defensins have low cytotoxicity to host cells and demonstrate immunomodulatory properties, and pathogens also display minimal resistance to these AMPs. Given the increasing concern in antimicrobial resistance, the potential of β-defensins as natural antimicrobials has garnered considerable attention. This article provides an overview of the characteristics of bovine β-defensins, their expression pathways, their mode of action, and factors influencing their expression in the mammary glands of cattle. Additionally, it identifies the current gaps in research within this field and suggests areas that require further investigation. Understanding the regulation and function of β-defensins offers valuable insights to develop effective strategies for strengthening the immune system of mammary glands, reducing the reliance on synthetic antimicrobials, and explore novel natural antimicrobial alternatives. Full article

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14 pages, 429 KiB

Open AccessArticle

Preoperative and Surgical Predictors of the Treatment Outcome of Dairy Cows with Right Abomasal Displacement—A Retrospective Study of 234 Cases

by Ioannis Proios and Walter Grünberg

Animals 2023, 13(18), 2887; https://doi.org/10.3390/ani13182887 - 11 Sep 2023

Cited by 1 | Viewed by 1673

Abstract

The displacement of the abomasum to the right (RDA) is a common condition regularly encountered in dairy cows, which requires urgent surgical correction. The survival of the patient primarily depends on early diagnosis and timely treatment, but other factors contributing to the outcome [...] Read more.

The displacement of the abomasum to the right (RDA) is a common condition regularly encountered in dairy cows, which requires urgent surgical correction. The survival of the patient primarily depends on early diagnosis and timely treatment, but other factors contributing to the outcome have been discussed in the literature. The objective of this study was to identify preoperative clinical, hematological, as well as intraoperative parameters that are associated with the prognosis of cows with RDA or abomasal volvulus (AV). This retrospective study included patients admitted to a veterinary teaching hospital over a period of 6 years with a diagnosis of RDA or AV. A total of 234 cows were included, of which 193 were discharged after treatment and thus classified as survivors. In contrast, 41 cases died or were euthanized during or after surgery and were categorized as non-survivors. Non-survivors showed more severe dehydration, higher heart rate, lower sodium, as well as higher L-lactate and phosphorus concentration in their blood prior to surgery compared with the survivors. During surgery, the abomasum of non-survivors was markedly dilated and twisted more frequently than in survivors. The results presented here can facilitate the early identification of animals with poor prognosis requiring more intensive peri- and postoperative care. Full article

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14 pages, 3315 KiB

Open AccessArticle

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

by Johan Manuel Calderón Bernal, Carlos Serna, Ángel García Muñoz, Alberto Díez Guerrier, Lucas Domínguez, José Francisco Fernández-Garayzábal, Ana Isabel Vela and Dolores Cid

Animals 2023, 13(17), 2687; https://doi.org/10.3390/ani13172687 - 22 Aug 2023

Cited by 1 | Viewed by 1416

Abstract

The aim of this study was to investigate the possible genotypic differences between commensal Pasteurella multocida isolates from apparently healthy animals (AHA) at the time of entry to feedlots and those from BRD-affected animals (BRD-AA). A total of 20 batches of beef calves [...] Read more.

The aim of this study was to investigate the possible genotypic differences between commensal Pasteurella multocida isolates from apparently healthy animals (AHA) at the time of entry to feedlots and those from BRD-affected animals (BRD-AA). A total of 20 batches of beef calves in seven feedlots were followed-up during the fattening period. P. multocida was isolated from 28.1% of AHA and 22.9% of BRD-AA. All isolates belonged to the A: L3 genotype. Most isolates from clinical cases (81.0%) grouped into a PFGE cluster were significantly associated with BRD cases (OR, 24.9; 95% CI, 6.4–96.2). The whole genomes of 14 isolates representative of the pulsotypes most frequently detected in BRD-AA and AHA were sequenced and compared with 53 bovine genomes belonging to the identified ST13, ST79, and ST80 genotypes for a global comparison. No differences were found in the virulence-associated gene content between sequence types (STs) globally or between BRD-AA and AHA isolates in this study. Significantly, ST79 isolates harbored ARGs, conferring resistance to different antimicrobials, including macrolides and tetracyclines, which are commonly used for the treatment of BRD. Two Spanish ST79 isolates carried an ICE highly similar to ICE Tn7407, which was recently detected in Germany, suggesting that ST79 P. multocida isolates in Europe and North America may be associated with different ICEs. Full article

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9 pages, 1643 KiB

Open AccessCase Report

Botulism in Cattle: A Case Report of an Outbreak in Sardinia (Italy)

by Luigia Pinna, Annamaria Coccollone, Marcella Maxia, Luca Bano, Concetta Scalfaro, Daniela Mandas and Manuele Liciardi

Animals 2023, 13(15), 2435; https://doi.org/10.3390/ani13152435 - 27 Jul 2023

Cited by 1 | Viewed by 2221

Abstract

Clostridium botulinum is the main causative agent of botulism in humans and animals. The ingestion of the botulinum neurotoxin, usually types C and D, has been shown to produce disease (neurological symptoms) in most botulism cases in cattle. We report an outbreak in [...] Read more.

Clostridium botulinum is the main causative agent of botulism in humans and animals. The ingestion of the botulinum neurotoxin, usually types C and D, has been shown to produce disease (neurological symptoms) in most botulism cases in cattle. We report an outbreak in Southern Sardinia that involved a livestock farm with 120 animals, 39 of which died. The aim of this report is to describe the course of this outbreak and the progression of symptoms up to the death of some animals; we also describe the therapeutic approach applied in this case and the analytical techniques used to diagnose the disease. Finally, we emphasize the importance of promptly proceeding with the sampling of several matrixes when a suspicion of botulism arises. Full article

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11 pages, 393 KiB

Open AccessArticle

A Comparison of Dry Period Outcomes after Selective Dry Cow Therapy Carried Out by Farm Staff versus Veterinary Students in a Low-Cell-Count Dairy Herd

by Peter Plate and Steven van Winden

Animals 2023, 13(14), 2318; https://doi.org/10.3390/ani13142318 - 15 Jul 2023

Viewed by 1157

Abstract

(1) Background: Selective dry cow therapy is widely promoted in many countries worldwide, however, concerns have been raised about the consequences of the unhygienic application of preparations by untrained operators, especially if no antimicrobials are being used, risking deteriorating mastitis outcomes. (2) Method: [...] Read more.

(1) Background: Selective dry cow therapy is widely promoted in many countries worldwide, however, concerns have been raised about the consequences of the unhygienic application of preparations by untrained operators, especially if no antimicrobials are being used, risking deteriorating mastitis outcomes. (2) Method: This study follows up on cows being dried off by farm staff and those dried off by final-year veterinary students and first-year graduate interns in a supervised training session. Subsequent mastitis parameters and culling data in a single herd with a low somatic cell count were evaluated. (3) Results: A total of 316 dry periods were enrolled in the study. There was no significant difference in the percentage of cows showing at least one high SCC reading within 90 days of the following lactation or cows with at least one case of clinical mastitis within the same period, neither in the total nor in the subset of cows dried off without an antimicrobial. Dry period cure rates and dry period new infection rates were similar too, as was the percentage of cows surviving in the herd after six months. The risk of culling within twelve months post-drying off was lower in cows dried off by students, the difference in survival manifesting itself from 150 days post-drying off, which is an unexplained finding. (4) Conclusion: Well-supervised practical training sessions on drying off routine can be responsibly implemented on well-managed commercial dairy herds. Full article

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12 pages, 2628 KiB

Open AccessArticle

Procalcitonin as an Endogenous Biomarker for Mastitis in Cows

by Stephan Neumann, Stephan Siegert and Anneke Fischer

Animals 2023, 13(13), 2204; https://doi.org/10.3390/ani13132204 - 5 Jul 2023

Cited by 2 | Viewed by 1581

Abstract

Mastitis is one of the most common diseases of dairy cows. Procalcitonin (PCT) has been described as an endogenous inflammatory biomarker for bacterial infections. The aim of this study was to find possible correlations between PCT concentrations in the serum and milk of [...] Read more.

Mastitis is one of the most common diseases of dairy cows. Procalcitonin (PCT) has been described as an endogenous inflammatory biomarker for bacterial infections. The aim of this study was to find possible correlations between PCT concentrations in the serum and milk of cows with mastitis and their clinical signs and disease progression. In total, 88 dairy cows were examined, of which 30 animals were diagnosed with clinical mastitis, 30 had subclinical mastitis, and 28 were designated as a healthy control group. The diseased animals were re-examined after 12 days. All PCT levels in this study were determined by a species-specific ELISA. All three groups could be differentiated from each other based on serum and milk PCT levels. The animals with clinical mastitis showed the highest mean concentrations of PCT (serum: 2641 pg/mL; milk: 1326 pg/mL), and the lowest PCT concentrations were found in the healthy control group (serum: 1166 pg/mL; milk: 176 pg/m). Over the course of the disease, results from the kinetics study showed that PCT levels remained high for the entire observation period. The results from this study showed that the PCT concentration could be used to differentiate between clinical mastitis, subclinical mastitis, and healthy cows. Full article

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9 pages, 540 KiB

Open AccessReview

Contagious Bovine Pleuropneumonia: A Passage to India

by Robin A. J. Nicholas

Animals 2023, 13(13), 2151; https://doi.org/10.3390/ani13132151 - 29 Jun 2023

Viewed by 1930

Abstract

The World Organization for Animal Health (WOAH)-listed contagious bovine pleuropneumonia (CBPP) emerged first in Europe and then spread to Eastern Asia, including Japan and China, from the Northern Territories of Australia at the end of the 19th century. Its route to India, however, [...] Read more.

The World Organization for Animal Health (WOAH)-listed contagious bovine pleuropneumonia (CBPP) emerged first in Europe and then spread to Eastern Asia, including Japan and China, from the Northern Territories of Australia at the end of the 19th century. Its route to India, however, is less well known as there is little evidence for large importations of cattle from Australia. The lack of accurate diagnostic tests at this time meant veterinary authorities relied solely on clinical and pathological signs, many of which were non-specific. Consequently, any diagnoses of CBPP reported in the early 20th century must be viewed with caution. More convincing reports of CBPP confirmed by laboratory tests were made in the 1930s and 1940s in the Indian state of Assam. Eradication campaigns began in the 1940s with immunizations of live attenuated vaccines and then more comprehensively in the 1950s and 1960s, supplemented with serological screening and the establishment of quarantine centres at international borders. The last case of CBPP, reported to WOAH, was seen in 1990, but the launch of a new awareness campaign in Assam in 2002 and recent reports of the disease in Pakistan suggests the disease has persisted in the Indian subcontinent well into the 21st century. Full article

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16 pages, 1269 KiB

Open AccessArticle

Treatment of Inactive Ovaries of Holstein Dairy Cows by Epidural Injection of GnRH Analogue (Receptal) and Its Impact on the Reproductive Hormones, Oxidant/Antioxidant Profile and Micro and Macro-Elements Profile

by Yahia A. Amin, Alaa Eldin Z. Mahmoud, Rana A. Ali, Samer S. Fouad, Obeid Shanab, Rawia M. Ibrahim, Foad Farrag, Mustafa Shukry, Samah F. Ibrahim, Liana Fericean and Ragab Hassan Mohamed

Animals 2023, 13(4), 653; https://doi.org/10.3390/ani13040653 - 13 Feb 2023

Cited by 3 | Viewed by 4179

Abstract

This study was designed to evaluate a new therapeutic approach for inactive ovaries based on the epidural administration of a GnRH agonist (Receptal) and an investigation of the impact of this treatment on the hormonal, oxidant/antioxidant and micro- and macro-element profiles. Sixty cows [...] Read more.

This study was designed to evaluate a new therapeutic approach for inactive ovaries based on the epidural administration of a GnRH agonist (Receptal) and an investigation of the impact of this treatment on the hormonal, oxidant/antioxidant and micro- and macro-element profiles. Sixty cows with postpartum anestrus were divided into two groups: the first group (group R_epid, n = 30) was administered an epidural injection of Receptal, while the second group (group C_epid, n = 30) received saline and was considered the control group. Evaluation of hormonal (progesterone, FSH, LH, testosterone, and cortisol), oxidant/antioxidant (MDA, SOD, GPx and TAC) as well as micro- and macroelement (calcium, phosphorus, manganese and magnesium) profiles was done in serum. The results showed that the epidural injection of Receptal has the potential to induce estrus response and conception incidence in treated cows. Compared to the control group, progesterone, FSH, and LH concentrations were significantly increased in the treated group, whereas testosterone and cortisol decreased (p < 0.05) following treatment. In addition, the treated group had greater TAC and GPx concentrations than the control group. Serum concentrations of magnesium increased (p < 0.05) following receptal treatment, but differences in other minerals were not detected. This research suggests a novel, effective method of treating inactive ovaries with epidural infusion of a GnRH agonist. Full article

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2022

Jump to: 2024, 2023, 2021, 2020

15 pages, 269 KiB

Open AccessArticle

Causes of Mortality of Dairy Cattle Diagnosed by Complete Necropsy

by David J. Wilson, Emma Jane Kelly and Savannah Gucwa

Animals 2022, 12(21), 3001; https://doi.org/10.3390/ani12213001 - 1 Nov 2022

Viewed by 3741

Abstract

This retrospective case series summarized the primary causes of death in 857 dairy cattle necropsied from 2008 to 2019 at the Utah Veterinary Diagnostic Laboratory, from dairy farms in Utah (76%), Idaho (16%) or other states (8%), primarily in the U.S. Intermountain West. [...] Read more.

This retrospective case series summarized the primary causes of death in 857 dairy cattle necropsied from 2008 to 2019 at the Utah Veterinary Diagnostic Laboratory, from dairy farms in Utah (76%), Idaho (16%) or other states (8%), primarily in the U.S. Intermountain West. Of cattle with age provided, 74% matched with body weight based estimates for those with no age stated. Cattle ranged from fetuses at 60 days of gestation to 9 years old. Primary cause of mortality was diagnosed in 833 cattle (97%); no cause was evident in 24 cattle (3%). Sexes were female 620 (72%), male 214 (25%), not recorded 23 (3%). Seven diseases killed 80% of the animals: gastrointestinal disease (most enteritis/colitis) 318 (37%); pneumonia 166 (19%); abortion 96 (11%), peritonitis 30 (4%), omphalophlebitis (navel ill) 27 (3%), abomasitis 23 (3%), and metritis 23 (3%). Etiologic agents and specific causes varied with age categories of the animals. Young calves that died from dystocia, omphalophlebitis, or congenital abnormality often presented with no suspicion of those causes by the owners because of no external signs. Some important fatal diseases of adult dairy cows that are often diagnosed ante-mortem were diagnosed at necropsy with no suspicion by those submitting the carcasses: metritis, hardware disease, and displaced abomasum. Multicentric lymphoma was a relatively important cause of death in cows more than 4 years old. Despite use of a toxicology laboratory, toxicity was only diagnosed as causing 1% of the deaths across all ages of dairy cattle. There were numerous other causes of mortality diagnosed as well. Necropsy is a vital tool to diagnose causes of death in dairy cattle and can guide changes in management or preventive practices to reduce the rate of deaths in dairy herds. Full article

18 pages, 9157 KiB

Open AccessArticle

In Vitro Gene Expression Responses of Bovine Rumen Epithelial Cells to Different pH Stresses

by Hongxia Lian, Chuankai Zhang, Yifan Liu, Wenjing Li, Tong Fu, Tengyun Gao and Liyang Zhang

Animals 2022, 12(19), 2621; https://doi.org/10.3390/ani12192621 - 29 Sep 2022

Viewed by 2198

Abstract

Ruminal acidosis often occurs in production, which greatly affects animal health and production efficiency. Subacute rumen acidosis (SARA) occurs when rumen pH drops rapidly to 5.5–5.8, and acute rumen acidosis (ARA) occurs when rumen pH drops below 5.0, but the molecular regulation mechanism [...] Read more.

Ruminal acidosis often occurs in production, which greatly affects animal health and production efficiency. Subacute rumen acidosis (SARA) occurs when rumen pH drops rapidly to 5.5–5.8, and acute rumen acidosis (ARA) occurs when rumen pH drops below 5.0, but the molecular regulation mechanism of the rumen epithelium after the rapid decrease in pH is still unclear. Bovine rumen epithelial cells (BRECs) were cultured at pH = 7.4 (control), 5.5 (SARA), and 4.5 (ARA). Transcriptome and metabolomic methods were used to obtain the molecular-based response of BRECs to different pH treatments; pH = 4.5 can significantly induce apoptosis of BRECs. The RNA-seq experiments revealed 1381 differently expressed genes (DEGs) in the control vs. SARA groups (p < 0.05). Fibroblast growth factor (FGF) and tumor necrosis factor (TNF) were upregulated 4.25 and 6.86 fold, respectively, and TLR4 was downregulated 0.58 fold. In addition, 283 DEGs were identified in the control vs. ARA comparison (p < 0.05), and prostaglandin-endoperoxide synthase 2 (PSTG2) was downregulated 0.54 fold. Our research reveals that the MAPK/TNF signaling pathway regulates the inflammatory response of BRECs. Metabolomics identified 35 biochemical compounds that were significantly affected (p < 0.05) in control vs. SARA and 51 in control vs. ARA. Bioinformatics analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database revealed that drug metabolism-cytochrome P450 metabolic and alpha-linolenic acid metabolism changes occurred. These transcriptional and metabolic changes are related to the adaptation of BRECs to low-pH stresses. In conclusion, the combined data analyses presented a worthy strategy to characterize the cellular, transcriptomic, and metabonomic adaptation of BRECs to pH in vitro. We demonstrated transcriptional expression changes in BRECs under pH stress and activation of the molecular mechanisms controlling inflammation. Full article

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8 pages, 1281 KiB

Open AccessArticle

Propagation of Babesia bigemina in Rabbit Model and Evaluation of Its Attenuation in Cross-Bred Calves

by Naimat Ullah, Kamran Ashraf, Abdul Rehman, Muhammad Suleman and Muhammad Imran Rashid

Animals 2022, 12(17), 2287; https://doi.org/10.3390/ani12172287 - 3 Sep 2022

Cited by 1 | Viewed by 1744

Abstract

Bovine babesiosis (BB) is a vector-borne disease (VBD) that affects cattle in tropical and subtropical areas, caused by the haemoprotozoa Babesia bovis and Babesia bigemina. It is transmitted by tick bites belonging to the genus Rhipicephalus and is clinically characterized by high [...] Read more.

Bovine babesiosis (BB) is a vector-borne disease (VBD) that affects cattle in tropical and subtropical areas, caused by the haemoprotozoa Babesia bovis and Babesia bigemina. It is transmitted by tick bites belonging to the genus Rhipicephalus and is clinically characterized by high fever, depression, anorexia, decreased milk and meat production, haemoglobinemia, haemoglobinuria, jaundice, and pregnancy loss. In this study, the propagation of B. bigemina was evaluated by intraperitoneally inoculating 3 × 10⁶ red blood cells infected with B. bigemina into rabbits. The study showed that variations in rabbit body temperatures are related to induced bovine babesiosis. A significant increase in temperature (39.20 ± 0.23 °C) was observed from day 4 onwards, with the maximum temperature (40.80 ± 1.01 °C) on day 9 post-inoculation. This study included susceptible cross-bred calves for in vivo attenuation, and they were compared with an infected group. The calves in the infected group showed a significant increase in temperature (38.79 ± 0.03 °C) from day 3 onwards and a maximum temperature (41.3 ± 0.17 °C) on day 11. Inoculated calves showed a gradual rise in temperature post-inoculation, but the difference was not significant. Inoculated calves did not show parasitaemia, whereas 32% of infected calves displayed parasitaemia. As compared to inoculated calves post-inoculation, packed cell volume (PCV) decreased (16.36 ± 1.30) for infected calves. However, there were statistically significant differences (p ≤ 0.05) in temperatures, parasitaemia, and PCV in both inoculated and infected calves. The current study aimed to attenuate B. bigemina in rabbit models and evaluate the pathogenic potential of this organism in naive calves. In conclusion, B. bigemina proliferation was attenuated in rabbits. The rabbit model can be used to study B. bigemina in vivo in order to reduce its pathogenicity. Full article

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10 pages, 1265 KiB

Open AccessArticle

Theileria annulata: Its Propagation in Rabbits for the Attenuation of Piroplasms in Cross-Bred Calves

by Muhammad Sajid Ramzan, Muhammad Imran Rashid, Haroon Akbar, Muhammad Avais and Muhammad Suleman

Animals 2022, 12(7), 813; https://doi.org/10.3390/ani12070813 - 23 Mar 2022

Cited by 5 | Viewed by 2511

Abstract

Tropical theileriosis caused by the protozoan; Theileria annulata is a tick-borne disease (TBD) transmitted by ticks of genus Hyalomma; is clinically characterized by fever, anemia, and lymphadenopathy; and is responsible for heavy economic losses in terms of high morbidity and mortality rates [...] Read more.

Tropical theileriosis caused by the protozoan; Theileria annulata is a tick-borne disease (TBD) transmitted by ticks of genus Hyalomma; is clinically characterized by fever, anemia, and lymphadenopathy; and is responsible for heavy economic losses in terms of high morbidity and mortality rates with reduced production. Infected red blood cells of T. annulata were inoculated into rabbits intraperitoneally, and propagation of T. annulata has been investigated. The current study has shown an association between induced tropical theileriosis and variation of body temperature in rabbits. A significant rise in temperature (39.92 ± 0.33 °C) was seen on day 8 onwards, with the maximum temperature (40.27 ± 0.44 °C) on day 14 post-inoculation. In the current study, in vivo trials in susceptible cross-bred calves to investigate the attenuation and comparison with the infected group were also conducted. All the infected calves (n = 5) showed a significant rise in temperature (40.26 ± 0.05 °C) on day 10 onwards, with the maximum temperature (40.88 ± 0.05 °C) on day 16. The temperature of inoculated calves increased gradually post-inoculation, but the difference was not significant. A maximum parasitemia of 20% was observed in infected calves, but no piroplasm parasitemia was observed in inoculated calves. The prescapular lymph nodes of infected calves were enlarged, while the lymph nodes of inoculated calves remained normal throughout the trial. Analysis of clinical and parasitological responses of infected and inoculated calves showed a significant difference (p ≤ 0.05) in terms of temperature, parasitemia, and lymph node scoring between two groups. The current study was primarily aimed to attenuate T. annulata in rabbit and to check its virulence in susceptible calves. It is concluded that propagation of Theileria annulata in rabbits made it attenuated. Rabbit can be used as an in vivo model to weaken the virulence of T. annulata. Full article

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2021

Jump to: 2024, 2023, 2022, 2020

10 pages, 271 KiB

Open AccessArticle

Infectious Bovine Respiratory Diseases in Adult Cattle: An Extensive Necropsic and Etiological Study

by Laëtitia Dorso, Maud Rouault, Claire Barbotin, Christophe Chartier and Sébastien Assié

Animals 2021, 11(8), 2280; https://doi.org/10.3390/ani11082280 - 2 Aug 2021

Cited by 8 | Viewed by 6985

Abstract

In young cattle, bovine respiratory disease (BRD) is a major cause of death and Mannheimia haemolytica is a frequent pathogen. Knowledge of fatal BRD in adult cattle is more limited. We assessed the importance of infectious BRD as a cause of death in [...] Read more.

In young cattle, bovine respiratory disease (BRD) is a major cause of death and Mannheimia haemolytica is a frequent pathogen. Knowledge of fatal BRD in adult cattle is more limited. We assessed the importance of infectious BRD as a cause of death in adult cattle and determined the associated pathogens. We analyzed data from 737 adult cattle necropsies at the Pathology Unit for Large Animals at Oniris, Nantes, France over a 6 year period (2013–2019). Each carcass was subjected to a complete necropsy. Lungs showing macroscopic lesions were classified into three categories: infectious primary pulmonary (IPP) lesions, thromboembolic pneumonia (TEP) and others (aspiration pneumonia, verminous pneumonia, and local extension of an extra-pulmonary inflammatory process). Half of the lungs with IPP macroscopic lesions were sampled for histology and submitted for polymerase chain reaction. BRD was the second leading cause of death (15.7%) after digestive diseases (32.2%). A strong predominance of IPP lesions (42.3%) and TEP lesions (39.6%) was also demonstrated. In IPP macroscopic lesions, fibrinous, hemorrhagic and/or hecrotic (FHN) bronchopneumonia accounted for 77.6% of macroscopic lesions. Mannheimia haemolytica was significantly associated with FHN bronchopneumonia macroscopic lesions. This study suggests that Mannheimia haemolytica should be included in the differential diagnosis of BRD in adult cattle. Full article

23 pages, 1852 KiB

Open AccessArticle

High Granulocyte-Macrophage Colony Stimulating Factor to Interleukin 10 Ratio and Marked Antioxidant Enzyme Activities Predominate in Symptomatic Cattle Naturally Infected with Candidatus Mycoplasma haemobos, Theileria orientalis, Theileria sinensis and Trypanosoma evansi

by Onyinyechukwu Ada Agina, Kim Tho Cheah, Nurul Syahirah Ahmad Sayuti, Mohd Rosly Shaari, Nur Mahiza Md Isa, Mokrish Ajat, Mohd Zamri-Saad, Mazlina Mazlan and Hazilawati Hamzah

Animals 2021, 11(8), 2235; https://doi.org/10.3390/ani11082235 - 29 Jul 2021

Cited by 3 | Viewed by 3751

Abstract

The aim of this study was to measure the serum proinflammatory (IL-12, GM-CSF & IFN-γ) to anti-inflammatory (IL-10, IL-4) cytokine ratio, oxidant (MDA) level and antioxidant enzyme (SOD; GPx) activities after blood parasite infections. The blood and serum samples were obtained from 130 [...] Read more.

The aim of this study was to measure the serum proinflammatory (IL-12, GM-CSF & IFN-γ) to anti-inflammatory (IL-10, IL-4) cytokine ratio, oxidant (MDA) level and antioxidant enzyme (SOD; GPx) activities after blood parasite infections. The blood and serum samples were obtained from 130 cattle and screened for identity of the infecting blood parasites by conventional PCR. The following blood parasite species were detected: Candidatus Mycoplasma haemobos (70/130); Theileria orientalis (65/130); Theileria sinensis (32/130); Anaplasma marginale (49/130); Anaplasma platys (7/130); and Trypanosoma evansi (4/130). The GM-CSF/IL-10 ratio showed significantly higher values in all the symptomatic blood parasite infected cattle groups except for symptomatic A. platys infected cattle groups. Anti-inflammatory cytokine immune responses were notable findings in symptomatic and asymptomatic cattle infected with C. M. haemobos and T. orientalis characterized by low serum IL-12:IL-10, IFN-γ:IL-10, IL-12:IL-4 and IFN-γ:IL-4 (p < 0.05). Therefore, high serum GM-CSF:IL:10 in the symptomatic blood parasite infected cattle, low serum IL-12:IL-10, IFN-γ:IL-10, IL-12:IL-4 and IFN-γ:IL-4 ratios in asymptomatic cattle, high MDA level, and increased antioxidant enzyme activities could be useful predictive tools for outcome of natural blood parasite infections in cattle. Full article

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10 pages, 257 KiB

Open AccessArticle

Identifying Health Status in Grazing Dairy Cows from Milk Mid-Infrared Spectroscopy by Using Machine Learning Methods

by Brenda Contla Hernández, Nicolas Lopez-Villalobos and Matthieu Vignes

Animals 2021, 11(8), 2154; https://doi.org/10.3390/ani11082154 - 21 Jul 2021

Cited by 17 | Viewed by 4961

Abstract

The early detection of health problems in dairy cattle is crucial to reduce economic losses. Mid-infrared (MIR) spectrometry has been used for identifying the composition of cow milk in routine tests. As such, it is a potential tool to detect diseases at an [...] Read more.

The early detection of health problems in dairy cattle is crucial to reduce economic losses. Mid-infrared (MIR) spectrometry has been used for identifying the composition of cow milk in routine tests. As such, it is a potential tool to detect diseases at an early stage. Partial least squares discriminant analysis (PLS-DA) has been widely applied to identify illness such as lameness by using MIR spectrometry data. However, this method suffers some limitations. In this study, a series of machine learning techniques—random forest, support vector machine, neural network (NN), convolutional neural network and ensemble models—were used to test the feasibility of identifying cow sickness from 1909 milk sample MIR spectra from Holstein-Friesian, Jersey and crossbreed cows under grazing conditions. PLS-DA was also performed to compare the results. The sick cow records had a time window of 21 days before and 7 days after the milk sample was analysed. NN showed a sensitivity of 61.74%, specificity of 97% and positive predicted value (PPV) of nearly 60%. Although the sensitivity of the PLS-DA was slightly higher than NN (65.6%), the specificity and PPV were lower (79.59% and 15.25%, respectively). This indicates that by using NN, it is possible to identify a health problem with a reasonable level of accuracy. Full article

12 pages, 236 KiB

Open AccessArticle

Changing Veterinary Attitudes towards Delivering Biosecurity Advice to Beef Farmers

by Barnaby Edmund Howarth and Steven van Winden

Animals 2021, 11(7), 1969; https://doi.org/10.3390/ani11071969 - 30 Jun 2021

Cited by 4 | Viewed by 3514

Abstract

Biosecurity advice is an important way veterinarians can help farmers to reduce disease burdens on their farms. Many different factors are at play when delivering this advice, one being veterinary competence and their communication skills. This study looked at the private veterinary practitioners’ [...] Read more.

Biosecurity advice is an important way veterinarians can help farmers to reduce disease burdens on their farms. Many different factors are at play when delivering this advice, one being veterinary competence and their communication skills. This study looked at the private veterinary practitioners’ perceptions of their own competence to deliver biosecurity advice as part of a longitudinal biosecurity project. Their responses were collected in the form of a telephone questionnaire. The results showed significant increases in private veterinary practitioners’ responses to comfort (p = 0.022), capability (p = 0.002), and consistency (p = 0.006) as well as an increase of uptake of advice (p = 0.015) as the project progressed. The mean time spent delivering biosecurity advice increased and dropped subsequently, suggesting an initially more thorough and later on a more efficient process. The overall perceptions of the veterinarians of the study were also assessed. The results suggest development of the participating veterinarians following the conscious-competence learning model showing a need to improve the knowledge and training of future generations of private veterinary practitioners in the area of biosecurity with, in particular, an increased focus on the importance of the veterinarian–farmer relationship. Full article

11 pages, 2990 KiB

Open AccessReview

Cryptosporidium spp. Infections in Combination with Other Enteric Pathogens in the Global Calf Population

by Beate Conrady, Michael Brunauer and Franz-Ferdinand Roch

Animals 2021, 11(6), 1786; https://doi.org/10.3390/ani11061786 - 15 Jun 2021

Cited by 8 | Viewed by 4827

Abstract

The most common worldwide diarrhoea-causing agents in neonatal calves are Cryptosporidium spp. (Crypto), bovine rotavirus (BRV), bovine coronavirus (BCoV), and enterotoxigenic Escherichia coli F5 (K99) (ETEC). Crypto is a zoonotic pathogen of diarrhoea in humans, particularly for children and immunocompromised adults. Four weighted-stratified [...] Read more.

The most common worldwide diarrhoea-causing agents in neonatal calves are Cryptosporidium spp. (Crypto), bovine rotavirus (BRV), bovine coronavirus (BCoV), and enterotoxigenic Escherichia coli F5 (K99) (ETEC). Crypto is a zoonotic pathogen of diarrhoea in humans, particularly for children and immunocompromised adults. Four weighted-stratified random-effect meta-analyses including meta-regression analyses were performed to calculate the worldwide mean prevalence of Crypto and associated concurrent infections with BRV, BCoV and ETEC, as well as their potential influencing factors. The meta-analysis incorporated 28 studies (56 substudies) in 17 countries that determined the presence or absence of concurrent infections with Crypto in the global calf population. Approximately half of all considered studies presented here were conducted in Europe independently of the type of infections with Crypto. Within Europe, the highest estimated mean Crypto-BRV prevalence was identified in Ireland (16.7%), the highest estimated mean Crypto-BCoV prevalence was detected in the United Kingdom (4.3%), and the highest estimated mean Crypto-ETEC prevalence across the literature was determined in Turkey (4.7%). The chance of detecting BRV, BCoV, and ETEC in calves with diarrhoea was 0.8 (confidence interval (CI): 0.6–1.0), 0.7 (CI: 0.5–1.0) and 0.6 (CI: 0.4–0.9) lower in the presence of Crypto compared to calves without Crypto. This may indicate an inhibitory effect between BRV, BCoV, ETEC, and Crypto in calves. The variance in the published prevalence across the literature can mainly be explained by the “diagnostic” factor (R² min–max: 0.0–40.3%), followed by the “health status of the sampled animals” (R² min–max: 1.4–27.3%) and “geographical region” (R² min–max: 5.9–23.6%). Full article

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11 pages, 1253 KiB

Open AccessArticle

Seroprevalence and Associated Risk Factors of Bovine Brucellosis in District Gujranwala, Punjab, Pakistan

by Muhammad Rashid Khan, Abdul Rehman, Salman Khalid, Mansur Ud Din Ahmad, Muhammad Avais, Mobeen Sarwar, Farhat Nazir Awan, Falk Melzer, Heinrich Neubauer and Tariq Jamil

Animals 2021, 11(6), 1744; https://doi.org/10.3390/ani11061744 - 11 Jun 2021

Cited by 6 | Viewed by 5340

Abstract

Bovine brucellosis is a contagious zoonotic disease that causes economic losses through abortion and infertility. A cross-sectional study was designed to determine the seroprevalence and associated risk factors of bovine brucellosis in district Gujranwala of Punjab, Pakistan. A total of 220 bovine sera [...] Read more.

Bovine brucellosis is a contagious zoonotic disease that causes economic losses through abortion and infertility. A cross-sectional study was designed to determine the seroprevalence and associated risk factors of bovine brucellosis in district Gujranwala of Punjab, Pakistan. A total of 220 bovine sera (112 from buffaloes, 108 from cattle) from 46 unvaccinated herds were collected. Parallel testing by the Rose Bengal Plate Test (RBPT) and Indirect Enzyme-linked Immunosorbent Assay (I-ELISA) showed a 58.7% (27/46) herd-level and 22.7% (50/220) animal-level seroprevalence. Seroprevalence was higher (p < 0.001, OR = 7.62) in adult animals (37.2%) compared to younger animals (4.9%). A herd size of >10 animals (p = 0.021, OR = 7.83), less housing space (p = 0.037, OR = 6.39) and history of abortion at the farm (p = 0.023, OR = 5.6) were found as risk factors associated with the seropositivity of brucellosis. There was a substantial agreement between the RBPT and I-ELISA results (Cohen’s kappa coefficient (κ) = 64.16, percent agreement = 89.5%). In conclusion, a relatively higher seroprevalence was found compared to the previous reports from the country. Standardization and validation of the advanced diagnostic tests would be needed. Biosecurity, personal protection, quarantine measures and routine screening of animals at the farm level and disease awareness programs and consumption of pasteurized milk in the human population will be helpful in preventing the transmission/zoonosis of the disease. Full article

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14 pages, 3479 KiB

Open AccessArticle

Longitudinal Metabolic Biomarker Profile of Hyperketonemic Cows from Dry-Off to Peak Lactation and Identification of Prognostic Classifiers

by Anna Mareike Couperus, Fabian Schroeder, Peter Hettegger, Johann Huber, Thomas Wittek and Johannes R. Peham

Animals 2021, 11(5), 1353; https://doi.org/10.3390/ani11051353 - 10 May 2021

Cited by 8 | Viewed by 2383

Abstract

Currently about 30% to 50% of all dairy cows are affected by a metabolic or infectious disease during the transition period. A key factor for preventive actions is the ability to precisely predict metabolic diseases at an early stage. We report the longitudinal [...] Read more.

Currently about 30% to 50% of all dairy cows are affected by a metabolic or infectious disease during the transition period. A key factor for preventive actions is the ability to precisely predict metabolic diseases at an early stage. We report the longitudinal metabolic profile of non-esterified fatty acids, beta-hydroxybutyrate (BHB), total bilirubin, and aspartate aminotransferase in hyperketonemic dairy cows. Aiming for a novel measurement regime to improve metabolic health in dairy cows, we evaluated prognostic classifiers for hyperketonemia. In the observational longitudinal study, 99 healthy adult primiparous and multiparous Simmental dairy cows were included. Every cow was monitored weekly for 14 consecutive weeks, beginning two weeks prior to the expected day of parturition until peak lactation. Cows with serum concentrations of BHB > 0.8 mmol/L were considered hyperketonemic. Biomarker profiles were fitted by the maximum likelihood method using a mixed effects natural cubic spline model. In the hyperketonemic group, the BHB profile remained significantly higher than that of the control group until the end of the study period. As a prognostic classifier, the cut-off level of 0.54 mmol/L BHB measured on the 10th day post partum had the highest area under the curve. These results provide new longitudinal insights into the metabolic biomarker progression of dairy cows and enable an early onset diagnosis of hyperketonemia. Full article

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14 pages, 1274 KiB

Open AccessArticle

The Specific Immune Response after Vaccination against Neonatal Calf Diarrhoea Differs between Apparent Similar Vaccines in a Case Study

by Román Gonzalez, Laura Elvira, Carlos Carbonell, Geert Vertenten and Lorenzo Fraile

Animals 2021, 11(5), 1238; https://doi.org/10.3390/ani11051238 - 25 Apr 2021

Cited by 4 | Viewed by 4400

Abstract

Neonatal calf diarrhoea (NCD) is a major health challenge with a negative impact on farm profitability, calf welfare and antimicrobial use. Neonatal calves are particularly sensitive to enteric infections. Thus, a key point for prevention is minimising infectious pressure and maximising specific immune [...] Read more.

Neonatal calf diarrhoea (NCD) is a major health challenge with a negative impact on farm profitability, calf welfare and antimicrobial use. Neonatal calves are particularly sensitive to enteric infections. Thus, a key point for prevention is minimising infectious pressure and maximising specific immune responses. An amount of 120 dams not previously vaccinated against NCD were randomly allocated to one of three study groups: negative control versus two vaccinated groups (A and B). In the control group, the average level of antibodies was significantly low for both BoCV and ETEC (15.6 and 13.9% in the colostrum samples, respectively), demonstrating the importance of dam vaccination. Indeed, the level of specific immunity was significantly increased for BoCV and ETEC with dam vaccination using both one-shot vaccines versus the control group. Moreover, the statistical analysis revealed a significantly higher level of antibodies for BoCV and ETEC in colostrum samples in vaccine A versus vaccine B and the control group. In accordance, the calf serum demonstrated a significantly higher level and greater homogeneity of antibodies against BoCV and ETEC in the Vaccine A group versus other experimental groups (p < 0.05). In conclusion, this study demonstrated a different specific immune response for the pathogens depending on the vaccine used to control NCD in cows. Full article

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9 pages, 496 KiB

Open AccessArticle

The Occurrence of Mycobacterium avium Subspecies paratuberculosis Positive Milk Antibody ELISA Results in Dairy Cattle under Varying Time Periods after Skin Testing for Bovine Tuberculosis

by Nicole Bridges and Steven van Winden

Animals 2021, 11(5), 1224; https://doi.org/10.3390/ani11051224 - 23 Apr 2021

Cited by 5 | Viewed by 2490

Abstract

Enzyme-linked immunosorbent assays (ELISA) are used to screen cows for Mycobacterium avium subspecies paratuberculosis (MAP) infections, informing Johne’s disease (JD) management practices in dairy herds. The causative agent of bovine tuberculosis (bTB), Mycobacterium bovis, and MAP share multiple antigens. Moreover, Mycobacterium avium [...] Read more.

Enzyme-linked immunosorbent assays (ELISA) are used to screen cows for Mycobacterium avium subspecies paratuberculosis (MAP) infections, informing Johne’s disease (JD) management practices in dairy herds. The causative agent of bovine tuberculosis (bTB), Mycobacterium bovis, and MAP share multiple antigens. Moreover, Mycobacterium avium subspecies avium is used in the single intradermal cervical comparative tests (SICCT) that are routinely used in early detection of cows infected with bTB. Although these are different types of immune responses, potentially the SICCT may interfere with the levels of MAP antibodies. This study aimed to clarify the relationship between the SICCT-MAP milk ELISA testing interval and apparent prevalence of JD risk statuses. Data from 51 herds were used, totalling 46,738 cow observations. The Poisson models showed that MAP milk ELISA testing at 14 day intervals post-SICCT statistically significantly increased the odds of detecting JD-positive cows compared to JD testing 85+ days post-SICCT. The odds ratio (OR) started at 2.5 in the first 14 day interval post-SICCT, increasing each two-week period to an OR of 4.0 at 57–70 days, to subsequently drop. Additionally, a herd history of bTB increased the odds of detecting JD-positive cows (OR = 1.2); this was relatively limited compared to the magnitude of the post-SICCT effect. Full article

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12 pages, 890 KiB

Open AccessArticle

A Retrospective Case Study into the Effect of Hoof Lesions on the Lying Behaviour of Holstein–Friesian in a Loose-Housed System

by Karen Jiewei Ji, Richard E. Booth and Nicola Blackie

Animals 2021, 11(4), 1120; https://doi.org/10.3390/ani11041120 - 14 Apr 2021

Cited by 4 | Viewed by 3079

Abstract

The association between hoof lesions and lying behaviour was assessed on a Holstein–Friesian dairy farm in England. Twenty-nine cows were included in the study. Cows with claw horn disruption lesions (CHDL, n = 8), soft tissue lesions (STL, n = 6), and no [...] Read more.

The association between hoof lesions and lying behaviour was assessed on a Holstein–Friesian dairy farm in England. Twenty-nine cows were included in the study. Cows with claw horn disruption lesions (CHDL, n = 8), soft tissue lesions (STL, n = 6), and no lesions (NL, n = 15) were assessed. Data were collected on parity, days in milk (DIM), and mobility scores. Cows were trimmed and treated, and lesions were recorded by a professional foot trimmer. Lying behaviour was assessed before and after claw trimming. The milking herd (n = 96) prevalence of lameness was 32.3%. Mobility was scored using the Agriculture and Horticulture Development Board (AHDB) Mobility Scoring system. Mobility scores were significantly different across lesions groups (p = 0.022). CHDL cows had a mean mobility score of 2.0 ± 0.9 (mean ± SD), STL were scored 1.2 ± 1.3, and NL cows were 0.9 ± 0.7. CHDL were associated with longer lying times (15.00 ± 1.04 h/d; p = 0.0006) and shorter standing times (9.68 ± 2.38 h/d; p = 0.0351) compared with NL lying times (11.77 ± 1.67 h/d) and standing times (12.21 ± 1.67 h/d). STL cows spent significantly less time lying (11.30 ± 2.44; p = 0.0013) than CHDL but not NL cows. No significant differences were found with any of the other lying behaviours. After trimming, CHDL cows spent significantly less time lying down than before trimming (13.66 ± 0.98; p = 0.0125). Cows with NL spent significantly more time lying down (12.57 ± 1.90; p = 0.0398) and had a shorter minimum lying bout duration (0.17 ± 0.09; p = 0.0236) after trimming. In conclusion, lying behaviour in dairy cattle was impacted by type of hoof lesions and hoof trimming. Full article

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14 pages, 795 KiB

Open AccessArticle

Reproductive Performance and Partial Budget Analysis of a Prostaglandin or a Modified Ovsynch Protocol in Autumn Calving Dairy Herds

by George Lindley, Jim Willshire and Steven Van Winden

Animals 2021, 11(4), 1031; https://doi.org/10.3390/ani11041031 - 6 Apr 2021

Cited by 1 | Viewed by 2616

Abstract

In autumn calving dairy herds, treatment of cattle not observed in estrus prior to the breeding season is common. Routinely, a single prostaglandin or a modified Ovsynch (MOFT) protocol are used—without evidence of their relative effectiveness. This study compares the effects on conception, [...] Read more.

In autumn calving dairy herds, treatment of cattle not observed in estrus prior to the breeding season is common. Routinely, a single prostaglandin or a modified Ovsynch (MOFT) protocol are used—without evidence of their relative effectiveness. This study compares the effects on conception, associated timing, and profitability of administering cows with prostaglandin or MOFT treatment. A hundred and ninety-two Holstein-Friesian cows from three herds without an observed estrus within 28-days before mating start date were randomly treated with d-cloprostenol (PGOD) or an 8-day MOFT protocol. The association of treatment and calving-breeding start-date interval (CBSI) on the risk of conception were investigated. Partial budget, sensitivity analysis, and Monte Carlo simulation was used to assess economic performance, identify critical input variables, and explore the effects of input uncertainties on model output. There was a significant association between MOFT treatment and conception during 21 and 84 days after mating start date, compared to PGOD. MOFT treatment was associated with a mean net benefit of £58.21 (sd £19.42) and £27.29 (sd £17.75) per cow for herds with a fixed or variable dry-off date, respectively. The relative profitability of an MOFT protocol is dependent on its effects on barren rate and herd dry-off strategy. Full article

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23 pages, 2352 KiB

Open AccessReview

Prevalence of Worldwide Neonatal Calf Diarrhoea Caused by Bovine Rotavirus in Combination with Bovine Coronavirus, Escherichia coli K99 and Cryptosporidium spp.: A Meta-Analysis

by Michael Brunauer, Franz-Ferdinand Roch and Beate Conrady

Animals 2021, 11(4), 1014; https://doi.org/10.3390/ani11041014 - 3 Apr 2021

Cited by 47 | Viewed by 8672

Abstract

Multiple enteropathogens such as bovine rotavirus (BRV), bovine coronavirus (BCoV), Escherichia coli K99 (ETEC) and Cryptosporidium spp. (Crypto) are the most common causes of calf diarrhoea during the first 30 days of animal age. Three weighted-stratified random-effects meta-analyses were performed to calculate the [...] Read more.

Multiple enteropathogens such as bovine rotavirus (BRV), bovine coronavirus (BCoV), Escherichia coli K99 (ETEC) and Cryptosporidium spp. (Crypto) are the most common causes of calf diarrhoea during the first 30 days of animal age. Three weighted-stratified random-effects meta-analyses were performed to calculate the worldwide prevalence of mixed infections of the causative agents (i.e., BRV-BCoV, BRV-ETEC, BRV-Crypto) and their potential influencing factors. The meta-analysis covered 41 studies (94 sub-studies) in 21 countries that determined the presence or absence of mixed infections in global calf populations. The highest worldwide estimated pooled prevalence was identified for BRV-Crypto (6.69%), followed by BRV-BCoV (2.84%), and BRV-ETEC (1.64%). The chance of detecting BCoV in calves with diarrhoea was 1.83 higher in the presence of BRV compared to calves without BRV, whereby an inhibition effect (odds ratio: 0.77) was determined between BRV and Crypto infections. The diagnostic methods were identified as a significant influencing factor in the detection of all considered mixed infections, while the other analysed factors differed in relation to their effect on prevalence. In contrast to BRV-BCoV, the prevalence of BRV-ETEC and BRV-Crypto mixed infections followed the course of individual ETEC and Crypto prevalence related to the age class of the sampled animals. Full article

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13 pages, 2778 KiB

Open AccessArticle

Leptospira Seroprevalence in Colombian Dairy Herds

by Simone Taddei, Giovanni Moreno, Clotilde Silvia Cabassi, Emiliana Schiano, Costanza Spadini and Sandro Cavirani

Animals 2021, 11(3), 785; https://doi.org/10.3390/ani11030785 - 11 Mar 2021

Cited by 7 | Viewed by 2883

Abstract

Leptospirosis in cattle has important economic effects on the infected farms. Moreover, livestock farming is considered a major occupational risk factor for the transmission of Leptospira infection to humans. A survey was performed to determine the overall and within-herd seroprevalence and mapping of [...] Read more.

Leptospirosis in cattle has important economic effects on the infected farms. Moreover, livestock farming is considered a major occupational risk factor for the transmission of Leptospira infection to humans. A survey was performed to determine the overall and within-herd seroprevalence and mapping of different Leptospira serovars in dairy cattle from farms located in some municipalities of the Colombian department of Boyacá. Nine hundred and fifty-nine animals, from 20 unvaccinated and one vaccinated herd, were included in the study. Anti-Leptospira serum antibodies were detected by the microscopic agglutination test (MAT). Only one herd was seronegative. Overall seroprevalence to at least one serovar of Leptospira was 24.1% for unvaccinated animals and 62.3% for animals from the vaccinated herd. A very high within-herd seroprevalence (>60%) was present in 20% of the unvaccinated herds. The presence in the vaccinated herd of 20/398 animals showing high titers, between 1000 and 4000, to at least one serovar of Leptospira suggest that some animals could have been infected. Moreover, due to the presence of seronegative animals, a failure of vaccination immunity or the presence of unvaccinated animals in the vaccinated herd cannot be excluded. In all farms, domestic animals other than cattle were present. Considering the farming practices occurring on dairy farms in the study area, higher hygienic standards and stricter biosecurity measures are suggested. Full article

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14 pages, 12576 KiB

Open AccessArticle

Relationship between Allelic Heterozygosity in BoLA-DRB3 and Proviral Loads in Bovine Leukemia Virus-Infected Cattle

by Hala El Daous, Shuya Mitoma, Eslam Elhanafy, Huyen Thi Nguyen, Ngan Thi Mai, Kosuke Notsu, Chiho Kaneko, Junzo Norimine and Satoshi Sekiguchi

Animals 2021, 11(3), 647; https://doi.org/10.3390/ani11030647 - 1 Mar 2021

Cited by 12 | Viewed by 2949

Abstract

Enzootic bovine leukosis is a lethal neoplastic disease caused by bovine leukemia virus (BLV), belongs to family Retroviridae. The BLV proviral load (PVL) represents the quantity of BLV genome that has integrated into the host’s genome in BLV-infected cells. Bovine leukocyte antigen ( [...] Read more.

Enzootic bovine leukosis is a lethal neoplastic disease caused by bovine leukemia virus (BLV), belongs to family Retroviridae. The BLV proviral load (PVL) represents the quantity of BLV genome that has integrated into the host’s genome in BLV-infected cells. Bovine leukocyte antigen (BoLA) class II allelic polymorphisms are associated with PVLs in BLV-infected cattle. We sought to identify relationships between BoLA-DRB3 allelic heterozygosity and BLV PVLs among different cattle breeds. Blood samples from 598 BLV-infected cattle were quantified to determine their PVLs by real-time polymerase chain reaction. The results were confirmed by a BLV-enzyme-linked immunosorbent assay. Restriction fragment length polymorphism-polymerase chain reaction identified 22 BoLA-DRB3 alleles. Multivariate negative binomial regression modeling was used to test for associations between BLV PVLs and BoLA-DRB3 alleles. BoLA-DRB3.2*3, *7, *8, *11, *22, *24, and *28 alleles were significantly associated with low PVLs. BoLA-DRB3.2*10 was significantly associated with high PVLs. Some heterozygous allele combinations were associated with low PVLs (*3/*28, *7/*8, *8/*11, *10/*11, and *11/*16); others were associated with high PVLs (*1/*41, *10/*16, *10/*41, *16/*27, and *22/*27). Interestingly, the BoLA-DRB3.2*11 heterozygous allele was always strongly and independently associated with low PVLs. This is the first reported evidence of an association between heterozygous allelic combinations and BLV PVLs. Full article

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2020

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16 pages, 6212 KiB

Open AccessArticle

Production Significance of Bovine Respiratory Disease Lesions in Slaughtered Beef Cattle

by Miguel Fernández, María del Carmen Ferreras, Francisco Javier Giráldez, Julio Benavides and Valentín Pérez

Animals 2020, 10(10), 1770; https://doi.org/10.3390/ani10101770 - 30 Sep 2020

Cited by 21 | Viewed by 7067

Abstract

Bovine respiratory disease (BRD) is still a serious concern in feedlots, where it exerts a negative effect on farm productivity. There is a shortage of studies focused on the evaluation of BRD-associated lesions at the slaughterhouse in clinically healthy animals. The objective of [...] Read more.

Bovine respiratory disease (BRD) is still a serious concern in feedlots, where it exerts a negative effect on farm productivity. There is a shortage of studies focused on the evaluation of BRD-associated lesions at the slaughterhouse in clinically healthy animals. The objective of this work was to investigate the prevalence and type of subclinical pneumonic lesions in slaughtered beef cattle, according to the age range and management system, and its impact on carcass weight. A total of 1101 beef cattle intended for human consumption were examined at slaughter. Information on age, sex, management system and carcass weight was recorded. The presence and type of pneumonia were evaluated according to gross and microscopic findings and etiological agents by PCR. Lung pneumonic lesions appeared in 17.9% of animals and were predominant among veal calves. According to the type, chronic catarrhal pneumonia prevailed in the majority of animals, and mixed and extensively reared cattle were more likely to suffer acute fibrinous pneumonia. The presence of pneumonic lesions was associated with a significant decrease in carcass weight that had more of an impact in veal male calves coming from intensive systems. Bacterial infections were the predominant infectious agent and the only cause of acute fibrinous pneumonia, while viruses were infrequent and only found in lesions with chronic catarrhal pneumonia. This study shows the importance of BRD in beef feedlots upon production values and points out the feasibility of slaughterhouse assessment of pneumonia as a method for the evaluation of BRD significance. Full article

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15 pages, 1134 KiB

Open AccessArticle

Veterinarians’ Knowledge, Attitudes and Practices Associated with Bovine Viral Diarrhoea Virus Control and Prevention in South-East Australia

by Claire McMorrow, Allan J. Gunn, Shahid Khalfan, Marta Hernandez-Jover and Victoria J. Brookes

Animals 2020, 10(9), 1630; https://doi.org/10.3390/ani10091630 - 11 Sep 2020

Cited by 9 | Viewed by 2683

Abstract

In Australia, the responsibility and associated costs for the control and prevention of Bovine Viral Diarrhoea Virus (BVDV) rest solely with producers. Veterinarians provide producers with farm-specific options for BVDV management and support BVDV control and elimination in their region. We surveyed veterinarians [...] Read more.

In Australia, the responsibility and associated costs for the control and prevention of Bovine Viral Diarrhoea Virus (BVDV) rest solely with producers. Veterinarians provide producers with farm-specific options for BVDV management and support BVDV control and elimination in their region. We surveyed veterinarians to determine their knowledge, attitudes and practices (KAP) associated with BVDV control in south-east Australia. We found that veterinarians’ recommendations do not always align with producers’ control measures. Veterinarians were uncertain about BVDV prevalence and the proportion of producers using BVDV control measures in their regions. Veterinarians generally promoted biosecurity and vaccination, and were concerned about the welfare and additional disease risks associated with persistently infected (PI) cattle. Veterinarians highlighted concerns about disease risks associated with a previously undocumented practice in which producers collect blood from PI cattle to administer to BVDV naïve cattle; termed “vampire vaccination” in this study. A greater understanding of the burden, impact and economics of BVDV is needed to align veterinarians’ and producers’ KAP to improve BVDV management on farms, and more appreciation of veterinarians’ and producers’ values is needed before BVDV control could be implemented at a regional or country level. Full article

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11 pages, 851 KiB

Open AccessArticle

The Effect of Staphylococcus spp., Streptococcus spp. and Enterobacteriaceae on the Development of Whey Protein Levels and Oxidative Stress Markers in Cows with Diagnosed Mastitis

by Kamila Puppel, Aleksandra Kalińska, Magdalena Kot, Jan Slósarz, Małgorzata Kunowska-Slósarz, Grzegorz Grodkowski, Beata Kuczyńska, Paweł Solarczyk, Tomasz Przysucha and Marcin Gołębiewski

Animals 2020, 10(9), 1591; https://doi.org/10.3390/ani10091591 - 7 Sep 2020

Cited by 4 | Viewed by 2715

Abstract

Mastitis is one of the most common diseases of high-yielding dairy cows, and over 90% of cases are caused by Streptococcus spp., Enterobacteriaceae, or Staphylococcus spp. Certain groups of proteins are very significant in terms of the cow’s antioxidant, bacteriostatic, and germicidal [...] Read more.

Mastitis is one of the most common diseases of high-yielding dairy cows, and over 90% of cases are caused by Streptococcus spp., Enterobacteriaceae, or Staphylococcus spp. Certain groups of proteins are very significant in terms of the cow’s antioxidant, bacteriostatic, and germicidal properties: lysozyme (Lz), lactoferrin (Lf), and β-lactoglobulin (BLG). This study aimed to determine the influence of Staphylococcus spp., Streptococcus spp., and Enterobacteriaceae on the secretion of bioactive whey proteins and oxidative stress markers. From the herd, 60 multiparous cows with diagnosed mastitis were selected. Samples were taken individually from each quarter and pooled, which gave 60 samples. Enterobacteriaceae did not affect the BLG synthesis, whereas lysozyme and lactoferrin responded to a high concentration of these bacterial strains. In the case of Staphylococcus spp. infection, the BLG level increased. These strains did not affect the levels of di-malonic aldehyde (MDA), lactoferrin, and lysozyme. In contrast, they were significantly influenced by Streptococcus spp. In summary, the levels of whey proteins and oxidative stress markers changed depending on the bacterial strain inducing inflammation. Lysozyme and lactoferrin may be markers of udder inflammation caused by Enterobacteriaceae and Streptococcus spp., whereas β-lactoglobulin may prove useful in diagnosing Staphylococcus spp. induced mastitis. Full article

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11 pages, 6125 KiB

Open AccessArticle

Characterization of Endolysin LyJH307 with Antimicrobial Activity against Streptococcus bovis

by Hanbeen Kim, Hyo Gun Lee, Inhyuk Kwon and Jakyeom Seo

Animals 2020, 10(6), 963; https://doi.org/10.3390/ani10060963 - 1 Jun 2020

Cited by 9 | Viewed by 3932

Abstract

Streptococcus bovis (S. bovis) is one of the critical initiators of acute acidosis in ruminants. Therefore, we aimed to develop and characterize the endolysin LyJH307, which can lyse ruminal S. bovis. We tested the bactericidal activity of recombinant LyJH307 against [...] Read more.

Streptococcus bovis (S. bovis) is one of the critical initiators of acute acidosis in ruminants. Therefore, we aimed to develop and characterize the endolysin LyJH307, which can lyse ruminal S. bovis. We tested the bactericidal activity of recombinant LyJH307 against S. bovis JB1 under a range of pH, temperature, NaCl, and metal ion concentrations. In silico analyses showed that LyJH307 has a modular design with a distinct, enzymatically active domain of the NLPC/P60 superfamily at the N-terminal and a cell wall binding domain of the Zoocin A target recognition domain (Zoocin A_TRD) superfamily at the C-terminal. The lytic activity of LyJH307 against S. bovis JB1 was the highest at pH 5.5, and relatively higher under acidic, than under alkaline conditions. LyJH307 activity was also the highest at 39 °C, but was maintained between 25°C and 55°C. LyJH307 bactericidal action was retained under 0-500 mM NaCl. While the activity of LyJH307 significantly decreased on treatment with ethylenediaminetetraacetic acid (EDTA), it was only restored with supplementation of 10 mM Ca²⁺. Analyses of antimicrobial spectra showed that LyJH307 lysed Lancefield groups D (S. bovis group and Enterococcus faecalis) and H (S. sanguinis) bacteria. Thus, LyJH307 might help to prevent acute ruminal acidosis. Full article

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