Animals

2023

Jump to: 2022, 2021, 2020

18 pages, 323 KiB

Open AccessArticle

Evaluation of Valorisation Strategies to Improve Spent Coffee Grounds’ Nutritional Value as an Ingredient for Ruminants’ Diets

by David San Martin, Jone Ibarruri, Nagore Luengo, Jorge Ferrer, Aser García-Rodríguez, Idoia Goiri, Raquel Atxaerandio, Mounir Medjadbi, Jaime Zufía, Estíbaliz Sáez de Cámara and Bruno Iñarra

Animals 2023, 13(9), 1477; https://doi.org/10.3390/ani13091477 - 26 Apr 2023

Cited by 3 | Viewed by 2300

Abstract

Lignin in animal diets is a limiting factor due to its low digestibility. This study assessed the effects of thermal or mechanical pre-treatments and enzymatic hydrolysis on spent coffee grounds’ (SCG) nutritional value and digestibility. A first trial studied the effect of thermal [...] Read more.

Lignin in animal diets is a limiting factor due to its low digestibility. This study assessed the effects of thermal or mechanical pre-treatments and enzymatic hydrolysis on spent coffee grounds’ (SCG) nutritional value and digestibility. A first trial studied the effect of thermal pre-treatment and hydrolysis with removal of the liquid part and a second trial studied mechanical pre-treatment and hydrolysis with and without removal of the liquid part. Autoclaving did not improve the enzymatic performance nor the nutritional value. Hydrolysis reduced the digestibility of the solid phase and impaired its ruminal fermentation efficiency. Hydrolysates without removing the liquid part improved its nutritional value, but not compared with unprocessed SCG. Grinding increased crude protein and reduced crude fibre and protein, which led to greater fermentation and in vitro digestibility. Thus, grinding emerges as the most promising valorisation strategy to improve SCG nutritional characteristics and their use for animal feed, contributing to the circular economy. Full article

16 pages, 599 KiB

Open AccessArticle

Vegetable By-Products as Alternative and Sustainable Raw Materials for Ruminant Feeding: Nutritive Evaluation and Their Inclusion in a Novel Ration for Calf Fattening

by Irantzu Goenaga, Aser García-Rodríguez, Idoia Goiri, Sara León-Ecay, Joana De Las Heras, Noelia Aldai and Kizkitza Insausti

Animals 2023, 13(8), 1391; https://doi.org/10.3390/ani13081391 - 18 Apr 2023

Cited by 5 | Viewed by 2727

Abstract

This research aimed to evaluate the nutritional composition, in vitro digestibility, and gas production kinetics of 15 vegetable by-products generated by the agri-food industry compared with corn silage as a reference raw material. Nutritional characterization and in vitro ruminal fermentation tests were performed [...] Read more.

This research aimed to evaluate the nutritional composition, in vitro digestibility, and gas production kinetics of 15 vegetable by-products generated by the agri-food industry compared with corn silage as a reference raw material. Nutritional characterization and in vitro ruminal fermentation tests were performed to determine in vitro organic matter digestibility and digestible energy values, short-chain fatty acids, and the gas production profile. Results indicate that vegetable by-products were more degradable, more extensively fermented, and fermented at a faster rate than corn silage. Going one step further in the valorization of these by-products in animal feed, the second part of the research aimed to compare the novel ration designed for calf fattening with a conventional one. An artificial rumen unit was used to obtain nutrient disappearance, rumen fermentation parameters, and gas production of rumen digesta. Very slight differences were observed between both experimental rations, with their composition being the main difference. Most of the unitary vegetable by-products and all mixes, as real examples of by-product generation in the agri-food industry, have higher digestibility and a greater nutritional value than corn silage. These by-products showed the potential to be used in ruminant-ensiled rations and could replace part of the ingredients in conventional diets. Full article

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14 pages, 310 KiB

Open AccessArticle

Effects of Sulla Flexuosa Hay as Alternative Feed Resource on Goat’s Milk Production and Quality

by Soumaya Boukrouh, Ali Noutfia, Nassim Moula, Claire Avril, Jean-Luc Hornick, Mouad Chentouf and Jean-François Cabaraux

Animals 2023, 13(4), 709; https://doi.org/10.3390/ani13040709 - 17 Feb 2023

Cited by 7 | Viewed by 1942

Abstract

Sulla flexuosa (Hedysarum flexuosum L.) is an endemic legume growing in some Mediterranean areas in rainfed and cold mountainous conditions. It could be used in goat diets as an alternative protein source instead of alfalfa to supplement forest rangeland. This study aimed [...] Read more.

Sulla flexuosa (Hedysarum flexuosum L.) is an endemic legume growing in some Mediterranean areas in rainfed and cold mountainous conditions. It could be used in goat diets as an alternative protein source instead of alfalfa to supplement forest rangeland. This study aimed to test the effects of incorporating Sulla flexuosa (SF) hay in the diet of Beni Arouss goats on their milk production and quality. The hay was introduced at two levels, i.e., 35 or 70% (SF70), on a DM basis; it partially or totally replaced the alfalfa hay of the control diet. Sulla flexuosa incorporation did not affect milk production or physicochemical composition. However, milk FA content varied in proportion to the percentage of SF incorporation. The SF70 diet was associated with increased milk levels in C18:1n-9, C18:2n-6, C18:3n-3, and C22:6n-3 and total monounsaturated, polyunsaturated, and n-3 fatty acids. As a consequence, atherogenic and thrombogenic indices were improved. Additionally, better antioxidant capacity was observed in SF70. Full article

18 pages, 325 KiB

Open AccessArticle

The Effects of Irrigation, Genotype and Additives on Tef Silage Making

by Philip Wagali, Chris Sabastian, Yehoshua Saranga, Shiran Ben-Zeev and Sameer J. Mabjeesh

Animals 2023, 13(3), 470; https://doi.org/10.3390/ani13030470 - 29 Jan 2023

Cited by 4 | Viewed by 2292

Abstract

Tef is known as a multi-harvest crop with high production capacity and outstanding fodder quality. Hence, our overall goal is to develop tef as a new multi-harvest summer crop that can maintain high-quality feed and contribute to both field crops and the livestock [...] Read more.

Tef is known as a multi-harvest crop with high production capacity and outstanding fodder quality. Hence, our overall goal is to develop tef as a new multi-harvest summer crop that can maintain high-quality feed and contribute to both field crops and the livestock industry in Israel. In this study, we aimed to evaluate the ability to preserve tef as silage. Four tef genotypes grown under well-watered (100%) and water-limited (75%) irrigation regimes were harvested at grain filling stage and ensiled with either no additives (control, CTL), or with heterofermentative inoculum (HI), molasses (MOL), and both MOL + HI. Our results showed for the first time that tef could be ensiled, although water-soluble carbohydrates (WSC) were lower than those in corn, “the perfect ensiling crop”. Most of the tef silage qualitative parameters were better at water-limited irrigation. Additives HI or MOL or MOL + HI also improved silage parameters, e.g., lowered pH and ammonia nitrogen content, but increased in vitro dry matter digestibility, lactic acid and crude protein content, and lactic acid bacteria counts of tef silage. The current results imply increasing the diversity of local ruminant fodder crops, ensuring high-quality feed supply during the summer. Full article

2022

Jump to: 2023, 2021, 2020

13 pages, 578 KiB

Open AccessArticle

Potential of Agroindustrial By-Products to Modulate Ruminal Fermentation and Reduce Methane Production: In Vitro Studies

by Carlos Navarro Marcos, Trinidad de Evan, Christian Jiménez and María Dolores Carro

Animals 2022, 12(24), 3540; https://doi.org/10.3390/ani12243540 - 15 Dec 2022

Viewed by 1808

Abstract

The effects of including wine lees (WL), exhausted olive cake (EOC) and a 1:1 mixture of EOC and tomato pomace (EOCTP) in diets for fattening ruminants on in vitro fermentation parameters and CH₄ production were analysed. Ten diets were studied, containing either [...] Read more.

The effects of including wine lees (WL), exhausted olive cake (EOC) and a 1:1 mixture of EOC and tomato pomace (EOCTP) in diets for fattening ruminants on in vitro fermentation parameters and CH₄ production were analysed. Ten diets were studied, containing either none of the tested by-products (control), or 6.0, 12.0 or 18.0% of WL, EOC and ECOTP formulated to have similar protein and fiber content. Diets were incubated in vitro with sheep ruminal fluid to measure gas production kinetics and fermentation parameters. Increasing the level of WL, EOC and EOCTP decreased linearly (p ≤ 0.009) the potential gas production, but other gas production parameters were unaffected (p > 0.05), excepting that EOCTP increased the gas production rate. No differences (p ≥ 0.0.05) among diets were observed in total volatile fatty acid (VFA) production at 24 h of incubation for EOC and EOCTP, but NH₃-N concentration decreased (p ≤ 0.003). In contrast, WL at 12.0 and 18.0% decreased (p < 0.05) total VFA production and increased the acetate/propionate ratio (p < 0.05). None of the by-products had an effect on CH₄ production (p ≥ 0.0.05). Results indicate that EOC and EOCTP could be included up to 18.0% in fattening diets, but lower levels of WL are recommended. Full article

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13 pages, 285 KiB

Open AccessArticle

Nutritional Values of Industrial Hemp Byproducts for Dairy Cattle

by Yiqiang Wang, Jianxu Gao, Chuanteng Cheng, Jingyi Lv, Modinat Tolani Lambo, Guangning Zhang, Yang Li and Yonggen Zhang

Animals 2022, 12(24), 3488; https://doi.org/10.3390/ani12243488 - 10 Dec 2022

Cited by 7 | Viewed by 2788

Abstract

The objective of this experiment was to explore the nutritional components of industrial hemp byproducts (industrial hemp ethanol extraction byproduct, IHEEB; industrial hemp stalk, IHS; industrial hemp seed meal, IHSM; industrial hemp oil filter residue, IHOFR) and provide theoretical support for the application [...] Read more.

The objective of this experiment was to explore the nutritional components of industrial hemp byproducts (industrial hemp ethanol extraction byproduct, IHEEB; industrial hemp stalk, IHS; industrial hemp seed meal, IHSM; industrial hemp oil filter residue, IHOFR) and provide theoretical support for the application of industrial hemp byproducts in dairy cattle production. This experiment used a combination of a wet chemical method with Cornell Net Carbohydrate and Protein System, in situ nylon bag technique, and three-step in vitro method to compare the chemical composition, carbohydrate and protein composition, in situ ruminal degradability and intestinal digestibility of industrial hemp byproducts and conventional feeds (alfalfa hay, AH; soybean meal, SBM). Available energy values were estimated based on the National Academies of Sciences, Engineering, and Medicine. The results showed that the nutritional composition of different feeds varied greatly. The two types of IHEEB were enriched with ash, crude protein (CP), neutral detergent fiber (NDF), and calcium, while the contents of neutral detergent insoluble crude protein, acid detergent insoluble crude protein, and acid detergent lignin were higher. As a result, the non-degradable carbohydrate and protein components were higher, and the effective degradation rate of rumen dry matter and protein was lower. IHS contains higher non-protein nitrogen and NDF, which enables it to provide more CP rumen effective degradation rate and carbohydrates, but the high acid detergent fiber also limits its application. IHSM possesses 296 g/kg CP and high rumen undegradable protein and intestinal digested protein, which can provide rumen bypass protein in dairy cows, making it a potentially good protein source. IHOFR had higher ether extract, rumen available protein degradation rate, and total tract digested protein, which can provide more energy and easily degradable protein for lactating cows. The available energy value of IHEEB and IHS was lower than AH, while SBM is between IHFOR and IHSM. In addition, the tetrahydrocannabinol of three industrial hemp byproducts that have not been assessed by the European Food Safety Authority (EFSA) was tested to evaluate their safety, and all of them were less than the limit set by ESFA. In conclusion, industrial hemp byproducts can be considered for inclusion in dietary formulations as unconventional feed sources for dairy cattle, but the purpose of use needs to be properly considered. Full article

13 pages, 332 KiB

Open AccessArticle

Dietary Effect of Palm Kernel Oil Inclusion in Feeding Finishing Lambs on Meat Quality

by Daniela Pionorio Vilaronga Castro, Paulo Roberto Silveira Pimentel, Neiri Jean Alves dos Santos, Jarbas Miguel da Silva Júnior, Gercino Ferreira Virginio Júnior, Ederson Américo de Andrade, Analívia Martins Barbosa, Elzânia Sales Pereira, Cláudio Vaz Di Mambro Ribeiro, Leilson Rocha Bezerra and Ronaldo Lopes Oliveira

Animals 2022, 12(23), 3242; https://doi.org/10.3390/ani12233242 - 23 Nov 2022

Cited by 2 | Viewed by 2192

Abstract

This study evaluated the effects of palm kernel oil (PKO) in the diet of lambs on carcass characteristics, quality, and fatty acid profile of the meat. Forty uncastrated male Santa Inês lambs were used and divided among the treatments: PKO_zero without inclusion; [...] Read more.

This study evaluated the effects of palm kernel oil (PKO) in the diet of lambs on carcass characteristics, quality, and fatty acid profile of the meat. Forty uncastrated male Santa Inês lambs were used and divided among the treatments: PKO_zero without inclusion; PKO_1.3—added 1.3%; PKO_2.6—added 2.6%; PKO_3.9—added 3.9%; PKO_5.2—added 5.2%. The carcass characteristics, the variables related to meat color, and the chemical composition of the Longissimus lumborum of lambs were not affected by the PKO inclusion. The weight of the carcasses at slaughter, hot and cold, half carcass, loin-eye area, and commercial cuts decreased linearly when PKO was added to the lamb diet (p < 0.01). CCY decreased linearly to the inclusion level of 2.66% PKO (RMSE 2.204). Total conjugated linoleic acid CLA and C18:3 n-3 GA concentrations remained stable until the inclusion levels of 3.44% PKO (RMSE 0.0956) and 2.17% (RMSE 0.0637), decreasing its concentrations as the increased level of PKO. The presence of PKO in the lambs’ diet up to the level of 5.2% did not change the meat quality characteristics; thus, from the point of view of lamb meat production and fatty acid profile, the inclusion of PKO is not beneficial. Full article

13 pages, 317 KiB

Open AccessArticle

Performance, Digestibility, Nitrogen Balance and Ingestive Behavior of Young Feedlot Bulls Supplemented with Palm Kernel Oil

by Neiri J. A. dos Santos, Leilson R. Bezerra, Daniela P. V. Castro, Polyana D. R. Marcelino, Ederson A. de Andrade, Gercino F. Virgínio Júnior, Jarbas M. da Silva Júnior, Elzânia S. Pereira, Analívia M. Barbosa and Ronaldo L. Oliveira

Animals 2022, 12(4), 429; https://doi.org/10.3390/ani12040429 - 11 Feb 2022

Cited by 6 | Viewed by 2589

Abstract

Vegetable oils can be used to increase energy density in diets; manipulate rumen fermentation; and alter the capacity for degradation, digestion and absorption of nutrients. Two experiments were conducted to evaluate palm kernel oil (PKO) in the diet of confined bulls with the [...] Read more.

Vegetable oils can be used to increase energy density in diets; manipulate rumen fermentation; and alter the capacity for degradation, digestion and absorption of nutrients. Two experiments were conducted to evaluate palm kernel oil (PKO) in the diet of confined bulls with the inclusion of 0.0, 11.5, 23.0 and 34.6 g PKO/kg dry matter (DM). The first experiment evaluated nutrient intake, performance, ingestive behavior and carcass characteristics. In the second experiment, steers crossbred with a ruminal cannula were used to evaluate digestibility, nitrogen balance, microbial protein synthesis, short-chain fatty acid levels and protozoal counts. The results showed that the inclusion of PKO linearly reduced intake in kg/day (DM, crude protein—CP, neutral detergent fiber—NDF_ap, nonfibrous carbohydrates—NFC and total digestible nutrient—TDN) and digestibility (DM, NDF_ap and TDN). Ether extract intake increased quadratically with the predicted maximum intake of 15.4 g/kg DM. Regarding ingestive behavior, there was a quadratic increase in rumination time and a quadratic reduction in idle time. Nitrogen balance, nitrogen intake, nitrogen retention, microbial protein production, acetate, butyrate, acetate/propionate ratio and protozoa count showed linear decreases due to dietary PKO inclusion. Regarding the carcass characteristics, linear decreases were observed for the final weight, average daily gain, hot carcass weight, cold carcass weight, hot carcass yield, cold carcass yield, loin eye area and subcutaneous fat thickness. The inclusion of PKO at up to 34.6 g/kg DM in diets for confined bulls reduces intake, negatively affecting digestibility, performance and carcass characteristics. Full article

12 pages, 303 KiB

Open AccessArticle

Inclusion Prosopis juliflora Pod Meal in Grazing Lambs Diets: Performance, Digestibility, Ingestive Behavior and Nitrogen Balance

by Bruna J. Almeida, Adriana R. Bagaldo, Mário S. F. Soares Junior, Cristiane S. da Silva, Fabiana L. de Araújo, Jarbas M. Silva Junior, Rosani V. M. M. Silva, Mailin V. S. Lima, Laudí C. Leite, Leilson R. Bezerra and Ronaldo L. Oliveira

Animals 2022, 12(4), 428; https://doi.org/10.3390/ani12040428 - 11 Feb 2022

Cited by 3 | Viewed by 2270

Abstract

Prosopis juliflora is an arboreal legume that concentrates its nutritive value in pods (fruits), constituting a rich source of carbohydrates and with a crude energy concentration comparable to ground corn. Therefore, we aimed to evaluate the effect of inclusion of Prosopis juliflora pod [...] Read more.

Prosopis juliflora is an arboreal legume that concentrates its nutritive value in pods (fruits), constituting a rich source of carbohydrates and with a crude energy concentration comparable to ground corn. Therefore, we aimed to evaluate the effect of inclusion of Prosopis juliflora pod meal (0 or control, 250, 500 and 750 g/kg total DM) as a replacement for ground corn in the diet of lambs on performance, digestibility, ingestive behavior, and nitrogen balance of lambs grazing in the Megathyrsus maximus pasture. Forty Santa Inês uncastrated lambs with an average body weight (BW) of 24.2 ± 0.55 kg were distributed in a completely randomized design. There was a linear increase in the non-fiber carbohydrates (NFC), ether extract (EE) and neutral detergent fiber (NDF) digestibility with Prosopis juliflora pod meal supplementation in the diet of the lambs. Dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) intake, and digestibility and time spent ruminating were not affected by Prosopis juliflora meal supplementation. Prosopis juliflora pod meal inclusion increased time spent feeding and idling of lambs, however, without affecting spent time ruminating. Lambs fed with Prosopis juliflora pod meal presented greater final BW, gain: feed ratio, N-balance, microbial protein production ef-ficiency, N-urea urinary (NUU) and blood urea nitrogen (BUN). However, the average daily weight (ADG), total weight gain and hot carcass yield as well as N ingested, N excreted in feces and urine, total purines, ab-sorbed purines and N microbial production in lambs were not influenced by Prosopis juliflora pod meal inclusion. The Prosopis juliflora pod meal inclusion up to the 750 g/kg level in the concentrate supplement totally replace ground corn in the diet of grazing lambs improving the NFC intake and NDF digestibility, supplement acceptability, microbial protein production efficiency and consequently the performance. The addition of Prosopis juliflora pod meal as a replacement for ground corn in the concentrate does not influence the microbial protein production; however, there was an improvement in the efficiency of microbial synthesis. Full article

13 pages, 1390 KiB

Open AccessArticle

Effects of Increasing Levels of Palm Kernel Oil in the Feed of Finishing Lambs

by Daniela Pionorio Vilaronga Castro, Paulo Roberto Silveira Pimentel, Jarbas Miguel da Silva Júnior, Gercino Ferreira Virgínio Júnior, Ederson Américo de Andrade, Analívia Martins Barbosa, Elzânia Sales Pereira, Claudio Vaz Di Mambro Ribeiro, Leilson Rocha Bezerra and Ronaldo Lopes Oliveira

Animals 2022, 12(4), 427; https://doi.org/10.3390/ani12040427 - 11 Feb 2022

Cited by 6 | Viewed by 2357

Abstract

The aim of this study was to evaluate the effects of the inclusion of palm kernel oil (PKO) in a lamb diet on nutrient intake, digestibility, ingestive behavior, nitrogen balance, blood metabolites, rumen fermentation parameters, and animal performance. Three experimental trials were conducted. [...] Read more.

The aim of this study was to evaluate the effects of the inclusion of palm kernel oil (PKO) in a lamb diet on nutrient intake, digestibility, ingestive behavior, nitrogen balance, blood metabolites, rumen fermentation parameters, and animal performance. Three experimental trials were conducted. The treatments consisted of varying levels of PKO included in the diet, with PKO_zero = no PKO inclusion, PKO_1.3 = 1.3% addition, PKO_2.6 = 2.6% addition, PKO_3.9 = 3.9% addition, and PKO_5.2 = 5.2% addition, based on the total dry matter (DM) of the diet. With the inclusion of PKO in the diet, linear decreases in DM (p < 0.001), crude ash (p < 0.001), crude protein (CP) (p < 0.001), neutral detergent fiber (NDF) (p < 0.001), nonfibrous carbohydrate (NFC) (p < 0.001), and total digestible nutrient (TDN) (p = 0.021) intake were observed, as was an increase in ether extract (EE) intake (p < 0.001). The digestibility coefficients of NDF and NFC were not affected by PKO addition to the diet. However, the digestibility of DM (p = 0.035), EE (p < 0.001), CP (p < 0.001), and TDNs (p < 0.001) increased when PKO was added to the lambs’ diet. Reductions in N intake (p < 0.001), fecal nitrogen excretion (p < 0.001), and microbial protein production (p < 0.001) were noted with increasing PKO levels. Serum cholesterol increased (p < 0.001) while serum GGT enzyme concentrations in the blood decreased (p = 0.048) with increasing PKO levels. PKO addition had no effect on total weight gain and average daily gain; however, feed conversion improved (p = 0.001) with increasing PKO levels. The intake, digestibility, ingestive behavior, and growth performance of lambs with PKO_1.3 added to their diet were similar to animals that did not receive PKO, meaning that PKO could be an alternative energy source for growing lambs because it does not harm animal performance and can lower the cost of feed. Full article

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2021

Jump to: 2023, 2022, 2020

13 pages, 529 KiB

Open AccessArticle

Effect of Fresh Citrus Pulp Supplementation on Animal Performance and Meat Quality of Feedlot Steers

by Santiago Luzardo, Georgget Banchero, Virginia Ferrari, Facundo Ibáñez, Gonzalo Roig, Valentín Aznárez, Juan Clariget and Alejandro La Manna

Animals 2021, 11(12), 3338; https://doi.org/10.3390/ani11123338 - 23 Nov 2021

Cited by 11 | Viewed by 3132

Abstract

The use of fruit by-products such as citrus pulp represents a feeding ingredient that deserves to be evaluated as an energy source in animal rations. Thirty-six British breed steers were allotted to one of the three feeding treatments (12 steers/treatment): 0%, 15% and [...] Read more.

The use of fruit by-products such as citrus pulp represents a feeding ingredient that deserves to be evaluated as an energy source in animal rations. Thirty-six British breed steers were allotted to one of the three feeding treatments (12 steers/treatment): 0%, 15% and 30% of fresh citrus pulp inclusion in the ration in a randomized complete block design to evaluate animal performance and carcass and meat quality traits. In the present study, the inclusion of fresh citrus pulp up to 30% of the diet did not affect the animal average daily gain (p > 0.05) but steers that were fed the pulp consumed less feed (p < 0.05) and presented a lower feed conversion ratio (p < 0.05) than their counterparts without citrus pulp in their diet. No effect of fresh citrus pulp was observed on carcass and meat quality (p > 0.05). A greater lipophilic antioxidant capacity (p < 0.05) in meat was observed when fresh citrus pulp was offered at 15% of the diet. Fresh citrus pulp used up to 30% as a feed ingredient in feedlot rations does not negatively affect animal performance or meat quality but, rather, has a positive effect on dry matter intake and a better feed conversion ratio. Full article

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11 pages, 308 KiB

Open AccessArticle

Solid State Fermentation as a Tool to Stabilize and Improve Nutritive Value of Fruit and Vegetable Discards: Effect on Nutritional Composition, In Vitro Ruminal Fermentation and Organic Matter Digestibility

by Jone Ibarruri, Idoia Goiri, Marta Cebrián and Aser García-Rodríguez

Animals 2021, 11(6), 1653; https://doi.org/10.3390/ani11061653 - 2 Jun 2021

Cited by 11 | Viewed by 5146

Abstract

This research aimed to evaluate in vitro organic matter digestibility, fermentation characteristics and methane production of fruit and vegetable discards processed by solid state fermentation (SSF) by Rhizopus sp. Mixtures were composed of approximately 28% citric fruits, 35% other fruits and 37% vegetables. [...] Read more.

This research aimed to evaluate in vitro organic matter digestibility, fermentation characteristics and methane production of fruit and vegetable discards processed by solid state fermentation (SSF) by Rhizopus sp. Mixtures were composed of approximately 28% citric fruits, 35% other fruits and 37% vegetables. Fruit and vegetables were processed and fermented to obtain a stabilized product. Nutritional characterization and in vitro ruminal fermentation tests were performed to determine the effect of fungal bioconversion on digestibility, end products and gas production kinetics. Results indicate that SSF reduced organic matter and reducing sugars, while it increased crude protein and neutral detergent fiber, acid detergent fiber and neutral detergent insoluble protein. The in vitro gas production showed that SSF led to a reduction of the organic matter digestibility (p < 0.001), short chain fatty acids (SCFA; p = 0.003) and CH₄ (p = 0.002). SSF reduced the gas production from the insoluble fraction (p = 0.001), without modifying the production rate (p = 0.676) or the lag time (p = 0.574). Regarding SCFA profile, SSF increased acetic (p = 0.020) and decreased propionic (p = 0.004) and butyric (p = 0.006) acids proportions, increasing acetic to propionic (p = 0.008) and acetic plus butyric to propionic (p = 0.011) ratios. SSF succeeded in obtaining a stabilized material enriched in protein, but at the expense of a reduction of protein availability and organic matter digestibility. These changes should be considered before including them in a ruminant’s rations. Full article

13 pages, 309 KiB

Open AccessArticle

Nutritive Value, In Vitro Fermentation, and Methane Production of Cactus Cladodes, Sugarcane Bagasse, and Urea

by Michelle Siqueira, Juana Chagas, João Paulo Monnerat, Carolina Monteiro, Robert Mora-Luna, José Dubeux, Jr., Nicolas DiLorenzo, Martin Ruiz-Moreno and Marcelo Ferreira

Animals 2021, 11(5), 1266; https://doi.org/10.3390/ani11051266 - 28 Apr 2021

Cited by 8 | Viewed by 2820

Abstract

This study evaluated the effect of different roughages fed to sheep on nutrient and water intake, ingestive behavior, nitrogen balance, microbial protein synthesis, fermentation parameters, and methane production using an in vitro gas production system. The treatments consisted of five diets: cactus cladodes [...] Read more.

This study evaluated the effect of different roughages fed to sheep on nutrient and water intake, ingestive behavior, nitrogen balance, microbial protein synthesis, fermentation parameters, and methane production using an in vitro gas production system. The treatments consisted of five diets: cactus cladodes Nopalea (NUB) and Opuntia (OUB), both with the addition of sugarcane bagasse (SB) and urea/ammonium sulfate (urea/as); Tifton hay (TH); corn silage (CS); and sorghum silage (SS), also with added urea/as. The NUB provided greater (p ≤ 0.03) intakes of dry matter (1024 g/d), digestible organic matter (670 g/d), and crude protein (161 g/d) than those on the SS. The NUB provided greater (p < 0.01) dietary water intake (3023 g/d) than TH. The time spent on rumination was shorter (p < 0.01), and the idle time was longer in animals fed NUB and OUB than TH and CS. Microbial protein synthesis was not affected (p = 0.27). The final pH (6.4) of the incubation fluid and the concentration of NH₃-N (39.05 mg/dL) were greater for NUB and OUB. Ruminal parameters and methane production were little or not affected by tested forages. We recommend using cactus cladodes in combination with sugarcane bagasse and urea/as in sheep diets. Full article

14 pages, 2624 KiB

Open AccessArticle

Effects of the Inclusion of Different Levels of Dietary Sunflower Hulls on the Colostrum Compositions of Ewes

by Mohsen M. Alobre, Mutassim M. Abdelrahman, Ibrahim A. Alhidary, Abdulrahman S. Alharthi and Riyadh S. Aljumaah

Animals 2021, 11(3), 777; https://doi.org/10.3390/ani11030777 - 11 Mar 2021

Cited by 4 | Viewed by 2376

Abstract

This study investigated the effects of supplementing different levels of sunflower hulls (SFH) to the complete feed of late-gestation pregnant ewes on the nutritive and fatty acids profile of colostrum at three and 48 h postpartum. In this study, 24 primiparous lactating Naemi [...] Read more.

This study investigated the effects of supplementing different levels of sunflower hulls (SFH) to the complete feed of late-gestation pregnant ewes on the nutritive and fatty acids profile of colostrum at three and 48 h postpartum. In this study, 24 primiparous lactating Naemi ewes were randomly selected at parturition from four dietary groups as follows: (1) C (control), total mixed ration (TMR; 0% SFH), (2) S12, TMR1 with 12% SFH (level 1), (3) S20, TMR2 with 20% SFH (level 2), and (4) S28, TMR3 with 28% SFH (level 3). The body condition scores were estimated 30 days prepartum. Colostrum samples were collected at three and 48 h postpartum to measure the nutritive values and fatty acids profiles. Data were analyzed as a complete randomized design with repeated measures and via Pearson’s correlation and regression analyses. The results indicated a numerical correlation (R² = 0.42; p < 0.09) between the body condition score and both colostrum fat and total solids. SFH increased the colostrum fat % (p < 0.05), especially for S12 and S20, following a cubic trend. Additionally, the colostrum from S12 and S20 ewes had a higher proportion of unsaturated fatty acids (USFAs), omega3 and lower levels of saturated fatty acids (SFAs), other than margaric acid (C17) and stearic acid (C18), SFA/USFA, and omega 6/omega 3. Furthermore, the regression analysis of the fatty acid classes and indices indicated a quadratic relationship between the parameters and SFH levels. The results confirm that the inclusion of SFH at levels greater than 20% may have a negative effect on some fatty acid parameters as a result of the high lignin intake. In conclusion, up to 20% SFH can be used in a complete feed for pregnant ewes without negative effects on the colostrum fat content and fatty acids profiles. Full article

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2020

Jump to: 2023, 2022, 2021

15 pages, 271 KiB

Open AccessArticle

Utilization of Avocado and Mango Fruit Wastes in Multi-Nutrient Blocks for Goats Feeding: In Vitro Evaluation

by Carlos Navarro Marcos, María Dolores Carro, Julia E. Fernández-Yepes, Lesly Arbesu and Eduarda Molina-Alcaide

Animals 2020, 10(12), 2279; https://doi.org/10.3390/ani10122279 - 3 Dec 2020

Cited by 15 | Viewed by 4015

Abstract

This study was conducted to investigate the nutritive value of avocado and mango fruit wastes, and to assess the possibility of preserving the wastes into multi-nutrient blocks (MB). Both peels and a pulp:peels (PP) mixture of each fruit were analyzed for chemical composition [...] Read more.

This study was conducted to investigate the nutritive value of avocado and mango fruit wastes, and to assess the possibility of preserving the wastes into multi-nutrient blocks (MB). Both peels and a pulp:peels (PP) mixture of each fruit were analyzed for chemical composition and in vitro fermentation with goats’ ruminal fluid. Wastes had low-dry matter (DM) content (<250 g/kg), with those from mango having high non-structural carbohydrates content (>800 g/kg DM) and those from avocado high fat levels (>580 g/kg DM). Mango wastes were fermented at a greater extent and faster rate than avocado ones. The PP mixture of each fruit was included into multi-nutrient blocks (MB) formulated to have similar chemical composition. There were only subtle differences in the fermentation of MB including wastes from either avocado or mango, but fermentation of avocado-MB resulted in significantly (p ≤ 0.032) greater acetate and lower propionate proportions than mango-MB. Including the PP mixture in the formulation of MB for goats feeding is a feasible option to reduce the environmental impact of avocado and mango fruit wastes, but studies on the acceptance of the MB by goats and their stability over long-time storage periods are needed. Full article

12 pages, 578 KiB

Open AccessArticle

Replacing Forage by Crude Olive Cake in a Dairy Sheep Diet: Effects on Ruminal Fermentation and Microbial Populations in Rusitec Fermenters

by Jairo García-Rodríguez, Iván Mateos, Cristina Saro, Jesús S. González, María Dolores Carro and María José Ranilla

Animals 2020, 10(12), 2235; https://doi.org/10.3390/ani10122235 - 28 Nov 2020

Cited by 9 | Viewed by 2918

Abstract

Olive oil extraction generates large amounts of a highly pollutant by-product called olive cake (OC), and its use in ruminant feeding could be an alternative. This study was designed to evaluate the effects of partially replacing forage by crude OC (COC) in a [...] Read more.

Olive oil extraction generates large amounts of a highly pollutant by-product called olive cake (OC), and its use in ruminant feeding could be an alternative. This study was designed to evaluate the effects of partially replacing forage by crude OC (COC) in a mixed dairy diet on rumen fermentation and microbial populations in Rusitec fermenters. The COC replaced 33% of the forage (66% maize silage and 33% barley straw) and was included at 16.6% of the total diet. Four fermenters were used in a cross-over design with two 13-day incubation periods. Experimental diets had a 50:50 forage-to-concentrate ratio and were formulated to contain the same protein (16.0%) and neutral detergent fiber (32.5%) levels. Compared with control fermenters, those fed the COC diet showed greater (p ≤ 0.02) pH (6.07 vs. 6.22), diet disappearance (0.709 vs. 0.748), and butyrate proportions (18.0 vs. 19.4), but there were no differences in volatile fatty acids and ammonia production. Microbial growth, bacterial diversity, protozoal abundance, and relative abundance of fungi and archaea were unaffected by diet, although the solid phase of COC-fed fermenters showed greater (p = 0.01) bacterial abundance than control ones. Results indicate that COC could replace 33% of the forage in a mixed dairy diet. Full article

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14 pages, 802 KiB

Open AccessArticle

In Vitro and In Situ Evaluation of Broccoli Wastes as Potential Feed for Ruminants

by Trinidad de Evan, Carlos N. Marcos, María José Ranilla and María Dolores Carro

Animals 2020, 10(11), 1989; https://doi.org/10.3390/ani10111989 - 29 Oct 2020

Cited by 13 | Viewed by 2807

Abstract

The potential of broccoli wastes (florets and stems) as ruminant feed was analyzed using in vitro and in situ techniques. Both stems and florets had high moisture content (90.6 and 86.1%, respectively), but the stems contained (% dry matter) lower levels (p [...] Read more.

The potential of broccoli wastes (florets and stems) as ruminant feed was analyzed using in vitro and in situ techniques. Both stems and florets had high moisture content (90.6 and 86.1%, respectively), but the stems contained (% dry matter) lower levels (p < 0.05) of crude protein (CP; 23.2 vs. 30.8%) and ether extract (2.91 vs. 6.15%) and tended to have greater sugars content (p = 0.071; 33.4 vs. 19.6%) than florets. Stems had greater in vitro dry matter rumen degradability (45.3%; 24 h incubation) and lower in vitro CP intestinal digestibility (82.7%) compared with florets (42.2 and 90.1%, respectively). Rumen degradability of protein was high (<85%) for both fractions. In a second experiment, diets including different proportions of broccoli were formulated and fermented in vitro. The replacement of 24% of conventional feeds (wheat, soybean meal and wheat bran) in a concentrate by dried broccoli increased the amount of organic matter fermented in vitro and the NH₃-N concentrations of a mixed diet including 40% of the concentrate. Including dried broccoli in the diet produced only small modifications in the volatile fatty acid profile and did not affect CH₄ emission. Full article

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18 pages, 343 KiB

Open AccessArticle

Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

by Margarita Novoa-Garrido, Carlos Navarro Marcos, María Dolores Carro Travieso, Eduarda Molina Alcaide, Mogens Larsen and Martin Riis Weisbjerg

Animals 2020, 10(11), 1957; https://doi.org/10.3390/ani10111957 - 23 Oct 2020

Cited by 14 | Viewed by 2908

Abstract

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and [...] Read more.

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH₃-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants. Full article

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