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16 pages, 7030 KiB

Open AccessArticle

Vertical Flow Immunoassay Based on Carbon Black Nanoparticles for the Detection of IgG against SARS-CoV-2 Spike Protein in Human Serum: Proof-of-Concept

by Maria Kropaneva, Pavel Khramtsov, Maria Bochkova, Sergey Lazarev, Dmitriy Kiselkov and Mikhail Rayev

Biosensors 2023, 13(9), 857; https://doi.org/10.3390/bios13090857 - 29 Aug 2023

Cited by 1 | Viewed by 2249

Abstract

Point-of-care tests play an important role in serological diagnostics of infectious diseases and post-vaccination immunity monitoring, including in COVID-19. Currently, lateral flow tests dominate in this area and show good analytical performance. However, studies to improve the effectiveness of such tests remain important. [...] Read more.

Point-of-care tests play an important role in serological diagnostics of infectious diseases and post-vaccination immunity monitoring, including in COVID-19. Currently, lateral flow tests dominate in this area and show good analytical performance. However, studies to improve the effectiveness of such tests remain important. In comparison with lateral flow tests, vertical flow immunoassays allow for a reduction in assay duration and the influence of the hook effect. Additionally, the use of carbon black nanoparticles (CNPs) as a color label can provide a lower detection limit (LOD) compared to conventional colloidal gold. Therefore, we have developed a vertical flow immunoassay for the detection of IgG against SARS-CoV-2 spike protein in human serum samples by applying a conjugate of CNPs with anti-human IgG mouse monoclonal antibodies (CNP@MAb). The vertical flow assay device consists of a plastic cassette with a hole on its top containing a nitrocellulose membrane coated with spike protein and an absorbent pad. The serum sample, washing buffer, and CNP@MAb flow vertically through the nitrocellulose membrane and absorbent pads, reducing assay time and simplifying the procedure. In positive samples, the interaction of CNP@MAb with anti-spike antibodies leads to the appearance of black spots, which can be visually detected. The developed method allows for rapid visual detection (5–7 min) of IgG vs. spike protein, with a LOD of 7.81 BAU/mL. It has been shown that an untrained operator can perform the assay and visually evaluate its results. Thus, the presented assay can be used in the further development of test systems for the serological diagnostics of COVID-19 or post-vaccination immunity monitoring. Full article

(This article belongs to the Special Issue Nanomaterial-Based Biosensors and Their Applications)

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17 pages, 5467 KiB

Open AccessArticle

Electrospun Nanofibers including Organic/Inorganic Nanohybrids: Polystyrene- and Clay-Based Architectures in Immunosensor Preparation for Serum Amyloid A

by Gizem Evren, Eray Er, Esra Evrim Yalcinkaya, Nesrin Horzum and Dilek Odaci

Biosensors 2023, 13(7), 673; https://doi.org/10.3390/bios13070673 - 23 Jun 2023

Cited by 5 | Viewed by 1667

Abstract

Diagnostic techniques based on biomolecules have application potential that can be realized in many fields, such as disease diagnosis, bioprocess imaging, food/beverage industries, and environmental pollutant imaging. Successful surface immobilization of biomolecules is critical to increasing the stabilization, sensitivity, and selectivity of biomolecules [...] Read more.

Diagnostic techniques based on biomolecules have application potential that can be realized in many fields, such as disease diagnosis, bioprocess imaging, food/beverage industries, and environmental pollutant imaging. Successful surface immobilization of biomolecules is critical to increasing the stabilization, sensitivity, and selectivity of biomolecules used in bioassay systems. Nanofibers are good candidates for the immobilization of biomolecules owing to many advantages such as morphology and pore size. In this study, montmorillonite (MMT) clay is modified with poly(amidoamine) (PAMAM) generation 3 (PAMAM_G3) and added to polystyrene (PS) solutions, following which PS/MMT-PAMAM_G3 nanofibers are obtained using the electrospinning method. The nanofibers are obtained by testing PS% (wt%) and MMT-PAMAM_G3% (wt%) ratios and characterized with scanning electron microscopy. Antiserum amyloid A antibody (Anti-SAA) is then conjugated to the nanofibers on the electrode surface via covalent bonds using a zero-length cross linker. Finally, the obtained selective surface is used for electrochemical determination of serum amyloid A (SAA) levels. The linear range of PS/MMT-PAMAM/Anti-SAA is between 1 and 200 ng/mL SAA, and the detection limit is 0.57 ng/mL SAA. The applicability of PS/MMT-PAMAM_G3/Anti-SAA is investigated by taking measurements in synthetic saliva and serum both containing SAA. Full article

(This article belongs to the Special Issue Nanomaterial-Based Biosensors and Their Applications)

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13 pages, 3678 KiB

Open AccessCommunication

Highly Sensitive Electrochemical Non-Enzymatic Uric Acid Sensor Based on Cobalt Oxide Puffy Balls-like Nanostructure

by Vandana Nagal, Sakeena Masrat, Marya Khan, Shamshad Alam, Akil Ahmad, Mohammed B. Alshammari, Kiesar Sideeq Bhat, Sergey M. Novikov, Prabhash Mishra, Ajit Khosla and Rafiq Ahmad

Biosensors 2023, 13(3), 375; https://doi.org/10.3390/bios13030375 - 12 Mar 2023

Cited by 23 | Viewed by 3247

Abstract

Early-stage uric acid (UA) abnormality detection is crucial for a healthy human. With the evolution of nanoscience, metal oxide nanostructure-based sensors have become a potential candidate for health monitoring due to their low-cost, easy-to-handle, and portability. Herein, we demonstrate the synthesis of puffy [...] Read more.

Early-stage uric acid (UA) abnormality detection is crucial for a healthy human. With the evolution of nanoscience, metal oxide nanostructure-based sensors have become a potential candidate for health monitoring due to their low-cost, easy-to-handle, and portability. Herein, we demonstrate the synthesis of puffy balls-like cobalt oxide nanostructure using a hydrothermal method and utilize them to modify the working electrode for non-enzymatic electrochemical sensor fabrication. The non-enzymatic electrochemical sensor was utilized for UA determination using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The puffy balls-shaped cobalt oxide nanostructure-modified glassy carbon (GC) electrode exhibited excellent electro-catalytic activity during UA detection. Interestingly, when we compared the sensitivity of non-enzymatic electrochemical UA sensors, the DPV technique resulted in high sensitivity (2158 µA/mM.cm²) compared to the CV technique (sensitivity = 307 µA/mM.cm²). The developed non-enzymatic electrochemical UA sensor showed good selectivity, stability, reproducibility, and applicability in the human serum. Moreover, this study indicates that the puffy balls-shaped cobalt oxide nanostructure can be utilized as electrode material for designing (bio)sensors to detect a specific analyte. Full article

(This article belongs to the Special Issue Nanomaterial-Based Biosensors and Their Applications)

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16 pages, 2471 KiB

Open AccessArticle

Reduced Graphene Oxide-Polydopamine-Gold Nanoparticles: A Ternary Nanocomposite-Based Electrochemical Genosensor for Rapid and Early Mycobacterium tuberculosis Detection

by Mansi Chaturvedi, Monika Patel, Neha Bisht, Shruti, Maumita Das Mukherjee, Archana Tiwari, D. P. Mondal, Avanish Kumar Srivastava, Neeraj Dwivedi and Chetna Dhand

Biosensors 2023, 13(3), 342; https://doi.org/10.3390/bios13030342 - 4 Mar 2023

Cited by 15 | Viewed by 2965

Abstract

Tuberculosis (TB) has been a devastating human illness for thousands of years. According to the WHO, around 10.4 million new cases of tuberculosis are identified every year, with 1.8 million deaths. To reduce these statistics and the mortality rate, an early and accurate [...] Read more.

Tuberculosis (TB) has been a devastating human illness for thousands of years. According to the WHO, around 10.4 million new cases of tuberculosis are identified every year, with 1.8 million deaths. To reduce these statistics and the mortality rate, an early and accurate TB diagnosis is essential. This study offers a highly sensitive and selective electrochemical biosensor for Mycobacterium tuberculosis (MTB) detection based on a ternary nanocomposite of reduced graphene oxide, polydopamine, and gold nanoparticles (rGO-PDA-AuNP). Avidin-biotin coupling was used to bind the MTB probe DNA onto the rGO-PDA-AuNP modified glassy carbon electrode (ssDNA/avidin/rGO-PDA-AuNP). UV-Visible, Raman, XRD, and TEM were used to evaluate the structural and morphological characteristics of rGO-PDA-AuNP. Furthermore, DNA immobilization is validated using FESEM and FT-IR techniques. The modified electrodes were electrochemically analyzed using cyclic voltammetry (CV) and linear sweep voltammetry (LSV), and the results indicate that the produced electrode can detect target DNA up to 0.1 × 10⁻⁷ mM with 2.12 × 10⁻³ mA µM⁻¹ sensitivity and a response time of 5 s. The constructed genosensor displayed high sensitivity and stability, and it also provides a unique strategy for diagnosing MTB at an early stage. Furthermore, our rGO-PDA-AuNP/GCE-based electrochemical platform has broad potential for creating biosensor systems for detecting various infectious pathogens and therapeutically significant biomarkers. Full article

(This article belongs to the Special Issue Nanomaterial-Based Biosensors and Their Applications)

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Review

Jump to: Research

36 pages, 1499 KiB

Open AccessReview

Different Aspects of the Voltammetric Detection of Vitamins: A Review

by Denise Kiamiloglou and Stella Girousi

Biosensors 2023, 13(6), 651; https://doi.org/10.3390/bios13060651 - 14 Jun 2023

Cited by 5 | Viewed by 2405

Abstract

Vitamins comprise a group of organic chemical compounds that contribute significantly to the normal functioning of living organisms. Although they are biosynthesized in living organisms, some are also obtained from the diet to meet the needs of organisms, which is why they are [...] Read more.

Vitamins comprise a group of organic chemical compounds that contribute significantly to the normal functioning of living organisms. Although they are biosynthesized in living organisms, some are also obtained from the diet to meet the needs of organisms, which is why they are characterized as essential chemical compounds. The lack, or low concentrations, of vitamins in the human body causes the development of metabolic dysfunctions, and for this reason their daily intake with food or as supplements, as well as the control of their levels, are necessary. The determination of vitamins is mainly accomplished by using analytical methods, such as chromatographic, spectroscopic, and spectrometric methods, while studies are carried out to develop new and faster methodologies and techniques for their analysis such as electroanalytical methods, the most common of which are voltammetry methods. In this work, a study is reported that was carried out on the determination of vitamins using both electroanalytical techniques, the common significant of which is the voltammetry technique that has been developed in recent years. Specifically, the present review presents a detailed bibliographic survey including, but not limited to, both electrode surfaces that have been modified with nanomaterials and serve as (bio)sensors as well as electrochemical detectors applied in the determination of vitamins. Full article

(This article belongs to the Special Issue Nanomaterial-Based Biosensors and Their Applications)

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32 pages, 2789 KiB

Open AccessReview

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

by Nur Melis Kilic, Sima Singh, Gulsu Keles, Stefano Cinti, Sevinc Kurbanoglu and Dilek Odaci

Biosensors 2023, 13(6), 622; https://doi.org/10.3390/bios13060622 - 5 Jun 2023

Cited by 16 | Viewed by 5064

Abstract

Electrochemistry is a genuinely interdisciplinary science that may be used in various physical, chemical, and biological domains. Moreover, using biosensors to quantify biological or biochemical processes is critical in medical, biological, and biotechnological applications. Nowadays, there are several electrochemical biosensors for various healthcare [...] Read more.

Electrochemistry is a genuinely interdisciplinary science that may be used in various physical, chemical, and biological domains. Moreover, using biosensors to quantify biological or biochemical processes is critical in medical, biological, and biotechnological applications. Nowadays, there are several electrochemical biosensors for various healthcare applications, such as for the determination of glucose, lactate, catecholamines, nucleic acid, uric acid, and so on. Enzyme-based analytical techniques rely on detecting the co-substrate or, more precisely, the products of a catalyzed reaction. The glucose oxidase enzyme is generally used in enzyme-based biosensors to measure glucose in tears, blood, etc. Moreover, among all nanomaterials, carbon-based nanomaterials have generally been utilized thanks to the unique properties of carbon. The sensitivity can be up to pM levels using enzyme-based nanobiosensor, and these sensors are very selective, as all enzymes are specific for their substrates. Furthermore, enzyme-based biosensors frequently have fast reaction times, allowing for real-time monitoring and analyses. These biosensors, however, have several drawbacks. Changes in temperature, pH, and other environmental factors can influence the stability and activity of the enzymes, affecting the reliability and repeatability of the readings. Additionally, the cost of the enzymes and their immobilization onto appropriate transducer surfaces might be prohibitively expensive, impeding the large-scale commercialization and widespread use of biosensors. This review discusses the design, detection, and immobilization techniques for enzyme-based electrochemical nanobiosensors, and recent applications in enzyme-based electrochemical studies are evaluated and tabulated. Full article

(This article belongs to the Special Issue Nanomaterial-Based Biosensors and Their Applications)

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27 pages, 3357 KiB

Open AccessReview

Advancements in Nanofiber-Based Electrochemical Biosensors for Diagnostic Applications

by Faiza Jan Iftikhar, Afzal Shah, Qamar Wali and Tayyaba Kokab

Biosensors 2023, 13(4), 416; https://doi.org/10.3390/bios13040416 - 23 Mar 2023

Cited by 13 | Viewed by 3286

Abstract

Biosensors are analytical tools that can be used as simple, real-time, and effective devices in clinical diagnosis, food analysis, and environmental monitoring. Nanoscale functional materials possess unique properties such as a large surface-to-volume ratio, making them useful for biomedical diagnostic purposes. Nanoengineering has [...] Read more.

Biosensors are analytical tools that can be used as simple, real-time, and effective devices in clinical diagnosis, food analysis, and environmental monitoring. Nanoscale functional materials possess unique properties such as a large surface-to-volume ratio, making them useful for biomedical diagnostic purposes. Nanoengineering has resulted in the increased use of nanoscale functional materials in biosensors. Various types of nanostructures i.e., 0D, 1D, 2D, and 3D, have been intensively employed to enhance biosensor selectivity, limit of detection, sensitivity, and speed of response time to display results. In particular, carbon nanotubes and nanofibers have been extensively employed in electrochemical biosensors, which have become an interdisciplinary frontier between material science and viral disease detection. This review provides an overview of the current research activities in nanofiber-based electrochemical biosensors for diagnostic purposes. The clinical applications of these nanobiosensors are also highlighted, along with a discussion of the future directions for these materials in diagnostics. The aim of this review is to stimulate a broader interest in developing nanofiber-based electrochemical biosensors and improving their applications in disease diagnosis. In this review, we summarize some of the most recent advances achieved in point of care (PoC) electrochemical biosensor applications, focusing on new materials and modifiers enabling biorecognition that have led to improved sensitivity, specificity, stability, and response time. Full article

(This article belongs to the Special Issue Nanomaterial-Based Biosensors and Their Applications)

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26 pages, 3627 KiB

Open AccessReview

Recent Prospects of Carbonaceous Nanomaterials-Based Laccase Biosensor for Electrochemical Detection of Phenolic Compounds

by Sakshi Verma, Deeksha Thakur, Chandra Mouli Pandey and Devendra Kumar

Biosensors 2023, 13(3), 305; https://doi.org/10.3390/bios13030305 - 22 Feb 2023

Cited by 14 | Viewed by 3358

Abstract

Phenolic compounds (PhCs) are ubiquitously distributed phytochemicals found in many plants, body fluids, food items, medicines, pesticides, dyes, etc. Many PhCs are priority pollutants that are highly toxic, teratogenic, and carcinogenic. Some of these are present in body fluids and affect metabolism, while [...] Read more.

Phenolic compounds (PhCs) are ubiquitously distributed phytochemicals found in many plants, body fluids, food items, medicines, pesticides, dyes, etc. Many PhCs are priority pollutants that are highly toxic, teratogenic, and carcinogenic. Some of these are present in body fluids and affect metabolism, while others possess numerous bioactive properties such as retaining antioxidant and antimicrobial activity in plants and food products. Therefore, there is an urgency for developing an effective, rapid, sensitive, and reliable tool for the analysis of these PhCs to address their environmental and health concern. In this context, carbonaceous nanomaterials have emerged as a promising material for the fabrication of electrochemical biosensors as they provide remarkable characteristics such as lightweight, high surface: volume, excellent conductivity, extraordinary tensile strength, and biocompatibility. This review outlines the current status of the applications of carbonaceous nanomaterials (CNTs, graphene, etc.) based enzymatic electrochemical biosensors for the detection of PhCs. Efforts have also been made to discuss the mechanism of action of the laccase enzyme for the detection of PhCs. The limitations, advanced emerging carbon-based material, current state of artificial intelligence in PhCs detection, and future scopes have also been summarized. Full article

(This article belongs to the Special Issue Nanomaterial-Based Biosensors and Their Applications)

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