Animals

17 pages, 1391 KiB

Open AccessArticle

The Effect of [(D-Ala⁶, Pro⁹NEt)mGnRH-a + Metoclopramide] (Ovopel) on Propagation Effectiveness of Two Breeding Lines of Common Carp (Cyprinus carpio L.) and on Luteinizing Hormone and 17α,20β-Dihydroxyprogesterone Levels in Females during Ovulation Induction

by Elżbieta Brzuska, Magdalena Socha, Jarosław Chyb, Mirosława Sokołowska-Mikołajczyk and Michał Inglot

Animals 2023, 13(8), 1428; https://doi.org/10.3390/ani13081428 - 21 Apr 2023

Cited by 1 | Viewed by 1614

Abstract

The study evaluates the impact of Ovopel on the reproductive effectiveness of carp from Polish line 6 and Lithuanian line B and the release of luteinizing hormone (LH) and 17α,20β-dihydroxyprogesterone (17α,20β-DHP) in females from these lines during ovulation induction. The levels of both [...] Read more.

The study evaluates the impact of Ovopel on the reproductive effectiveness of carp from Polish line 6 and Lithuanian line B and the release of luteinizing hormone (LH) and 17α,20β-dihydroxyprogesterone (17α,20β-DHP) in females from these lines during ovulation induction. The levels of both hormones were determined in blood plasma samples taken just before the priming injection of Ovopel (0 h), at the time of administering the resolving dose of Ovopel (12 h), and after the next 12 h (24 h). Following Ovopel treatment, the mean egg weight obtained for line 6 was higher, but not statistically different, than that observed for line B. Egg quality, on the other hand, was significantly higher in line B. Female provenance did not significantly affect the number of eggs and living embryos after 70 h incubation. However, the total egg number for line 6 was higher. The mean number of living embryos (70 h) was similar for both lines. LH concentrations at 0, 12, and 24 h were not statistically different between the lines. A comparison of LH concentrations between ovulated and non-ovulated females at different sampling times revealed no significant differences either within or between the lines. Statistically significant differences in LH levels were found for both ovulated and non-ovulated females from a given line between the sampling times. The results for 17α,20β-DHP were similar, with only one difference: 24 h after the priming dose of Ovopel, 17α,20β-DHP levels in ovulated fish were significantly higher compared with non-ovulated females, but only in line 6. Full article

(This article belongs to the Section Aquatic Animals)

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14 pages, 4585 KiB

Open AccessArticle

Life History Parameters and Fishing Aspects of the Alien Nimble Spray Crab Percnon gibbesi in a Native Area of the Central-East Atlantic

by Airam Guerra-Marrero, Antonio Bonino-Pérez, Ana Espino-Ruano, Lorena Couce-Montero, David Jiménez-Alvarado and José J. Castro

Animals 2023, 13(8), 1427; https://doi.org/10.3390/ani13081427 - 21 Apr 2023

Cited by 3 | Viewed by 2169

Abstract

Percnon gibbesi is a native crab species characteristic of intertidal and subtidal zones of the Atlantic coast of the European Macaronesian archipelagos (Azores, Madeira, and Canary Islands), and probably also in the neighbouring rocky coasts of northwest Africa. P. gibbesi is considered an [...] Read more.

Percnon gibbesi is a native crab species characteristic of intertidal and subtidal zones of the Atlantic coast of the European Macaronesian archipelagos (Azores, Madeira, and Canary Islands), and probably also in the neighbouring rocky coasts of northwest Africa. P. gibbesi is considered an invasive alien species in almost all of the Mediterranean, with expanding populations from Spain to Turkey, including Libya. However, its biology and ecology are highly unknown, despite all its range of distribution. In the intertidal zones of Gran Canaria Island, this crab, in the intertidal zones of Gran Canaria Island, shows a carapace length range between 4.1 and 22.7 mm (4.1–22.7 in males and 5.7–22.3 in females), where females showed higher weights and lengths than males on average. However, males predominated in all samples, with a sex ratio of 1:0.57. The L_∞ for this crab was estimated to be 27 ± 3 mm (23 ± 4 mm for females and 25 ± 4 mm for males). The growth coefficient (K) was 0.24 year⁻¹, the total mortality (Z) was Z = 1.71 year⁻¹, and the natural mortality (M) was 0.47 year⁻¹_. Although females grow faster than males, males are more abundant in the larger length classes. Although the presence of ovigerous females indicated that reproduction takes place twice a year, from March to April and from August to September, the number of cohorts detected by the modal progression analysis showed that reproduction takes place all year. Full article

(This article belongs to the Special Issue Aquatic Fauna Biodiversity and Conservation)

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17 pages, 960 KiB

Open AccessArticle

Different Conditions during Confinement in Pasture-Based Systems and Feeding Systems Affect the Fatty Acid Profile in the Milk and Cheese of Holstein Dairy Cows

by Lucía Grille, Daniela Escobar, Maria Noel Méndez, María de Lourdes Adrien, Laura Olazabal, Víctor Rodríguez, Ronny Pelaggio, Pablo Chilibroste, Ana Meikle and Juan Pablo Damián

Animals 2023, 13(8), 1426; https://doi.org/10.3390/ani13081426 - 21 Apr 2023

Cited by 2 | Viewed by 2014

Abstract

The diet of dairy cows influences the fatty acid (FA) profiles of their milk and cheese, but how these are affected by different conditions during confinement in a mixed system (MS:grazing + total mixed ration:TMR) is not known. The aim of this study [...] Read more.

The diet of dairy cows influences the fatty acid (FA) profiles of their milk and cheese, but how these are affected by different conditions during confinement in a mixed system (MS:grazing + total mixed ration:TMR) is not known. The aim of this study was to compare the FAs of the milk and cheese from MS in a compost-bedded pack barns (CB-GRZ) versus an outdoor soil-bedded pen (OD-GRZ) during confinement, and with a confinement system (100%TMR) in a compost-bedded pack barns (CB-TMR). Individual milk samples (n = 12 cows/group), cheese, and pooled milk (MilkP) samples were collected. The saturated FA percentages in the milk and the omega 6/omega 3 ratio in the MilkP and cheese were greater for the CB-TMR (p < 0.0001), while the unsaturated and monounsaturated FA percentages in the milk were lower for the CB-TMR than the MS (p < 0.001). The milk n-3, C18:3, and conjugated linoleic acid percentages were lower for the CB-TMR than the MS (p < 0.001). The milk n-3 and C18:3 were higher for the CB-GRZ than the OD-GRZ (p < 0.01), but no differences were observed between the MS in the MilkP and cheese. In conclusion, CB-GRZ cows during confinement produced better quality milk compared to OD-GRZ cows. However, the FA profiles of the milk, MilkP, and cheese were affected to a greater extent by the feeding management than by the conditions during confinement. Full article

(This article belongs to the Special Issue Factors Affecting Milk Productivity, Milk Quality, and Dairy Cow Health)

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13 pages, 1247 KiB

Open AccessReview

Infrared Thermography as a Potential Non-Invasive Tool for Estrus Detection in Cattle and Buffaloes

by Umair Riaz, Musadiq Idris, Mehboob Ahmed, Farah Ali and Liguo Yang

Animals 2023, 13(8), 1425; https://doi.org/10.3390/ani13081425 - 21 Apr 2023

Cited by 9 | Viewed by 3950

Abstract

The productivity of dairy animals has significantly increased over the past few decades due to intense genetic selection. However, the enhanced yield performance of milk animals caused a proportional increase in stress and compromised reproductive efficiency. Optimal reproductive performance is mandatory for the [...] Read more.

The productivity of dairy animals has significantly increased over the past few decades due to intense genetic selection. However, the enhanced yield performance of milk animals caused a proportional increase in stress and compromised reproductive efficiency. Optimal reproductive performance is mandatory for the sustainable production of dairy animals. Reproductive efficiency is marked by proper estrus detection and precise breeding to achieve maximum pregnancies. The existing conventional methods of estrus detection are somewhat labor intensive and less efficient. Similarly, the modern automated methods that rely on detecting physical activity are expensive, and their efficiency is affected by factors such as type of housing (tie stall), flooring, and environment. Infrared thermography has recently emerged as a technique that does not depend on monitoring physical activity. Furthermore, infrared thermography is a non-invasive, user-friendly, and stress-free option that aids in the detection of estrus in dairy animals. Infrared thermography has the potential to be considered a useful non-invasive tool for detecting temperature fluctuations to generate estrus alerts without physical contact in cattle and buffaloes. This manuscript highlights the potential use of infrared thermography to understand reproductive physiology and practical implementation of this technique through discussing its advantages, limitations, and possible precautions. Full article

(This article belongs to the Special Issue Livestock Reproduction: Reproductive Technologies in Animal Science)

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16 pages, 2040 KiB

Open AccessArticle

Validation of Enzyme Immunoassays via an Adrenocorticotrophic Stimulation Test for the Non-Invasive Quantification of Stress-Related Hormone Metabolites in Naked Mole-Rats

by Tshepiso Lesedi Majelantle, Nigel Charles Bennett, Stefanie Birgit Ganswindt, Daniel William Hart and Andre Ganswindt

Animals 2023, 13(8), 1424; https://doi.org/10.3390/ani13081424 - 21 Apr 2023

Cited by 1 | Viewed by 1869

Abstract

Small size in mammals usually restricts long-term, frequent monitoring of endocrine function using plasma as a matrix. Thus, the non-invasive monitoring of hormone metabolite concentrations in excreta may provide an invaluable approach. The aim of the current study was to examine the suitability [...] Read more.

Small size in mammals usually restricts long-term, frequent monitoring of endocrine function using plasma as a matrix. Thus, the non-invasive monitoring of hormone metabolite concentrations in excreta may provide an invaluable approach. The aim of the current study was to examine the suitability of enzyme immunoassays (EIAs) for monitoring responses to stressors in the naked mole-rat (Heterocephalus glaber, NMR) using urine and feces as hormone matrices. A saline control administration, and a high- and low-dose adrenocorticotropic hormone (ACTH) challenge were performed on six male and six female disperser morph NMRs. The results revealed that a 5α-pregnane-3β,11β,21-triol-20-one EIA detecting glucocorticoid metabolites (GCMs) with a 5α-3β-11β-diol structure is the most suitable assay for measuring concentrations in male urine samples, whereas an 11-oxoaetiocholanolone EIA detecting GCMs with a 5β-3α-ol-11-one structure appears the most suitable EIA for quantifying GCMs in female urine. An 11-oxoaetiocholanolone EIA detecting 11,17 dioxoandrostanes was the most suitable EIA for quantifying GCMs in the feces of both sexes. There were sex-related differences in response to the high- and low-dose ACTH challenge. We recommend using feces as a more suitable matrix for non-invasive GCM monitoring for NMRs which can be valuable when investigating housing conditions and other welfare aspects. Full article

(This article belongs to the Special Issue Non-invasive Methods of Stress Monitoring in Animals under Human Care)

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19 pages, 331 KiB

Open AccessReview

Promoting Good Nonhuman Primate Welfare outside Regular Working Hours

by Sabrina Brando, Augusto Vitale and Madison Bacon

Animals 2023, 13(8), 1423; https://doi.org/10.3390/ani13081423 - 21 Apr 2023

Cited by 3 | Viewed by 3651

Abstract

Promoting good primate welfare outside of daylight hours is an important task. The responsibility to provide a complex environment and environmental enrichment is an essential element of primate wellbeing programs that should be approached from a 24-h perspective and planned according to the [...] Read more.

Promoting good primate welfare outside of daylight hours is an important task. The responsibility to provide a complex environment and environmental enrichment is an essential element of primate wellbeing programs that should be approached from a 24-h perspective and planned according to the species and individual needs, including giving animals the ability to interact with and control their environment during hours when animal care staff are not present. One must be aware, however, that their needs may differ at night-time from their care needs during the day when staff are present. Assessing welfare and providing enrichment during times when staff are not on hand can be achieved through the use of a variety of technologies, such as night-view cameras, animal-centred technologies, and data loggers. This paper will address the relevant topics concerning the care and welfare of primates during off-hours, and the use of related technologies to facilitate and assess wellbeing at these times. Full article

(This article belongs to the Special Issue Care Strategies of Non-Human Primates in Captivity)

18 pages, 305 KiB

Open AccessArticle

Mandan, Hidatsa, and Arikara Nation Perspectives on Rez Dogs on the Fort Berthold Reservation in North Dakota, U.S.A.

by Alexandra Cardona, Sloane M. Hawes, Jeannine Cull, Katherine Connolly, Kaleigh M. O’Reilly, Liana R. Moss, Sarah M. Bexell, Michael Yellow Bird and Kevin N. Morris

Animals 2023, 13(8), 1422; https://doi.org/10.3390/ani13081422 - 21 Apr 2023

Cited by 2 | Viewed by 4296

Abstract

The research on the relationships between free-roaming dogs, also referred to as reservation dogs or rez dogs, and Indigenous communities is extremely limited. This study aimed to document the cultural significance of rez dogs, challenges related to rez dogs, and community-specific solutions for [...] Read more.

The research on the relationships between free-roaming dogs, also referred to as reservation dogs or rez dogs, and Indigenous communities is extremely limited. This study aimed to document the cultural significance of rez dogs, challenges related to rez dogs, and community-specific solutions for rez dog issues affecting community health and safety from members of the Mandan, Hidatsa, and Arikara (MHA) Nation, also referred to as the Three Affiliated Tribes (TAT), who live on the Fort Berthold reservation in North Dakota, U.S.A. One hour semi-structured interviews with 14 community members of the MHA Nation were conducted in 2016. The interviews were analyzed via systematic and inductive coding using Gadamer’s hermeneutical phenomenology. The primary intervention areas described by the participants included: culturally relevant information sharing, improved animal control policies and practices, and improved access to veterinary care and other animal services. Full article

(This article belongs to the Special Issue Animal Welfare from a Cross-Cultural Perspective)

15 pages, 2306 KiB

Open AccessArticle

Defining an Optimal Range of Centrifugation Parameters for Canine Semen Processing

by Nicole Sugai, Stephen Werre, Julie Cecere and Orsolya Balogh

Animals 2023, 13(8), 1421; https://doi.org/10.3390/ani13081421 - 21 Apr 2023

Cited by 3 | Viewed by 1776

Abstract

Our objective was to determine a clinically relevant range of centrifugation parameters for processing canine semen. We hypothesized that higher gravitational (g) force and longer time of centrifugation would result in improved spermatozoa recovery rate (RR) but poorer semen quality. Cooled storage under [...] Read more.

Our objective was to determine a clinically relevant range of centrifugation parameters for processing canine semen. We hypothesized that higher gravitational (g) force and longer time of centrifugation would result in improved spermatozoa recovery rate (RR) but poorer semen quality. Cooled storage under standard shipping conditions was used as a stressor to evaluate long-term treatment effects. Individual ejaculates collected from 14 healthy dogs were split into six treatment groups (400 g, 720 g, and 900 g for 5 or 10 min). Sperm RR (%) was calculated post-centrifugation, and plasma membrane integrity (%, Nucleocounter^® SP-100™), total and progressive motility (%, subjective and computer-assisted sperm analysis), and morphology (%, eosin-nigrosin staining) were assessed on initial raw semen (T0), post-centrifugation (T1), and 24 h (T2) and 48 h (T3) after cooling. Sperm losses were minimal, and RRs were similar across treatment groups (median >98%, p ≥ 0.062). Spermatozoa membrane integrity was not different between centrifugation groups at any time point (p ≥ 0.38) but declined significantly during cooling (T1 vs. T2/T3, p ≤ 0.001). Similarly, total and progressive motility did not differ across treatments but declined in all groups from T1 to T3 (p ≤ 0.02). In conclusion, our study showed that centrifugation within a range of 400 g–900 g for 5–10 min is appropriate for processing canine semen. Full article

(This article belongs to the Section Animal Reproduction)

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20 pages, 6152 KiB

Open AccessArticle

Agreement between Electrical Cardiometry and Pulmonary Artery Thermodilution for Measuring Cardiac Output in Isoflurane-Anesthetized Dogs

by Vaidehi V. Paranjape, Natalia Henao-Guerrero, Giulio Menciotti, Siddharth Saksena and Manuela Agostinho

Animals 2023, 13(8), 1420; https://doi.org/10.3390/ani13081420 - 21 Apr 2023

Cited by 4 | Viewed by 2355

Abstract

In animals, invasive pulmonary artery thermodilution (PATD) is a gold standard for cardiac output (CO) monitoring, but it is impractical in clinical settings. This study evaluates the agreement between PATD and noninvasive electrical cardiometry (EC) for measuring CO and analyzes the other EC-derived [...] Read more.

In animals, invasive pulmonary artery thermodilution (PATD) is a gold standard for cardiac output (CO) monitoring, but it is impractical in clinical settings. This study evaluates the agreement between PATD and noninvasive electrical cardiometry (EC) for measuring CO and analyzes the other EC-derived hemodynamic variables in six healthy anesthetized dogs subjected to four different hemodynamic events in a sequential order: (1) euvolemia (baseline); (2) hemorrhage (33% blood volume loss); (3) autologous blood transfusion; and (4) 20 mL/kg colloid bolus. The CO measurements obtained using PATD and EC are compared using Bland–Altman analysis, Lin’s concordance correlation (LCC), and polar plot analysis. Values of p < 0.05 are considered significant. The EC measurements consistently underpredict the CO values as compared with PATD, and the LCC is 0.65. The EC’s performance is better during hemorrhage, thus indicating its capability in detecting absolute hypovolemia in clinical settings. Even though the percentage error exhibited by EC is 49.4%, which is higher than the standard (<30%), EC displays a good trending ability. Additionally, the EC-derived variables display a significant correlation with the CO measured using PATD. Noninvasive EC may have a potential in monitoring trends in hemodynamics in clinical settings. Full article

(This article belongs to the Special Issue Anaesthesia and Analgesia in Companion Animals)

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13 pages, 1939 KiB

Open AccessArticle

Frequency and Characterisation of Anomalies and Fractures of the Caudal Spine in Sheep with Undocked Tails

by Hannah Hümmelchen, Henrik Wagner, Kerstin Brügemann, Sabine Wenisch, Sven König and Axel Wehrend

Animals 2023, 13(8), 1419; https://doi.org/10.3390/ani13081419 - 21 Apr 2023

Cited by 2 | Viewed by 1932

Abstract

As tails are often docked within the first days of life, studies investigating tail malformations and injuries in sheep do not exist thus far. To address this gap in the literature, this research aimed to analyse the occurrence of vertebral anomalies and fractures [...] Read more.

As tails are often docked within the first days of life, studies investigating tail malformations and injuries in sheep do not exist thus far. To address this gap in the literature, this research aimed to analyse the occurrence of vertebral anomalies and fractures in the tail within an undocked Merinoland sheep population. At 14 weeks of age, the caudal spines of 216 undocked Merinoland lambs was radiographically examined, and tail length and circumference were measured. Anomalies were documented and statistical correlation and model calculations were performed. The occurrence of block vertebrae was observed in 12.96% and wedged vertebrae in 8.33% of the sample. Of the animals, 59 (27.31%) exhibited at least one vertebral fracture, which were observed in the middle and caudal third of the tail. A significant correlation was found between the occurrence of fractures and tail length (r = 0.168) and number of vertebrae (r = 0.155). Conversely, the presence of block and wedged vertebrae was not significantly correlated with tail length, circumference, or number of vertebrae. Only the sex showed significant differences in the probability of axis deviation. These results emphasize the importance of breeding for short tails to avoid fractures. Full article

(This article belongs to the Section Animal Welfare)

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20 pages, 1558 KiB

Open AccessArticle

Effects of High Concentrate-Induced Subacute Ruminal Acidosis Severity on Claw Health in First-Lactation Holstein Cows

by Johann Kofler, Michael Hoefler, Thomas Hartinger, Ezequias Castillo-Lopez, Johann Huber, Alexander Tichy, Nicole Reisinger and Qendrim Zebeli

Animals 2023, 13(8), 1418; https://doi.org/10.3390/ani13081418 - 20 Apr 2023

Cited by 5 | Viewed by 2772

Abstract

This study aimed to evaluate the effects of diet-induced subacute rumen acidosis (SARA) severity during transition and the early lactation period on claw health in 24 first-lactation Holstein heifers. All heifers were fed a 30% concentrate (in dry matter) close-up ration three weeks [...] Read more.

This study aimed to evaluate the effects of diet-induced subacute rumen acidosis (SARA) severity during transition and the early lactation period on claw health in 24 first-lactation Holstein heifers. All heifers were fed a 30% concentrate (in dry matter) close-up ration three weeks before calving, then switched to a high-concentrate ration (60% dry matter), which was fed until the 70th day in milk (DIM) to induce SARA. Thereafter, all cows were fed the same post-SARA ration with around 36% concentrate in dry matter. Hoof trimming was performed before calving (visit 1), at 70 (visit 2) and at 160 DIM (visit 3). All claw lesions were recorded, and a Cow Claw Score (CCS) was calculated for each cow. Locomotion scores (LCS 1–5) were assessed at two-week intervals. Intraruminal sensors for continuous pH measurements were used to determine SARA (pH below 5.8 for more than 330 min in 24 h). The cluster analysis grouped the cows retrospectively into light (≤11%; n = 9), moderate (>11–<30%; n = 7), and severe (>30%; n = 8) SARA groups, based on the percentage of days individual cows experienced SARA. Statistically significant differences were found between SARA groups light and severe in terms of lameness incidence (p = 0.023), but not for LCS and claw lesion prevalence. Further, the analysis of maximum likelihood estimates revealed that for each day experiencing SARA, the likelihood of becoming lame increased by 2.52% (p = 0.0257). A significant increase in white line lesion prevalence was observed between visits 2 and 3 in the severe SARA group. The mean CCS in severe SARA group cows were higher at each visit compared to cows in the other two groups, but without statistical significance. Overall, this is the first study indicating that first-lactation cows fed a similar high-concentrate diet but with a higher severity of SARA tended to have poorer claw health, albeit with only partial statistical evidence. Full article

(This article belongs to the Section Cattle)

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19 pages, 1731 KiB

Open AccessArticle

Diet Preference, Feed Efficiency and Expression of the Sodium-Dependent Glucose Transporter Isoform 1 and Sweet Taste Receptors in the Jejunum of Lambs Supplemented with Different Flavours

by Felista Mwangi, Areen Dallasheh, Mugagga Kalyesubula, Naama Reicher, Chris Sabastian and Sameer J. Mabjeesh

Animals 2023, 13(8), 1417; https://doi.org/10.3390/ani13081417 - 20 Apr 2023

Cited by 1 | Viewed by 2433

Abstract

This study investigated the effect of dietary flavour supplements on the preference, feed efficiency and expression of the sweet taste receptor family 1 members 2 and 3 (T1R2 + T1R3), and sodium-glucose linked transporter 1 (SGLT1) genes in the [...] Read more.

This study investigated the effect of dietary flavour supplements on the preference, feed efficiency and expression of the sweet taste receptor family 1 members 2 and 3 (T1R2 + T1R3), and sodium-glucose linked transporter 1 (SGLT1) genes in the lambs’ small intestines. Eight, five-month-old, Israeli crossbred Assaf lambs were offered 16 different non-nutritive commercial flavours in rolled barley and ground corn. Capsicum and sucram were the most preferred non-aroma flavours (p = 0.020), while milky (p < 0.001) was the most preferred powder-aroma flavour. For the metabolic and relative gene expression study, eight lambs were randomly assigned to either sucram, capsicum, a mix containing sucram and capsicum at 1:1 ratio or no flavour for control in a 4 × 2 cross-over design. The total collection of urine (females only), faeces and refusals was carried out, and T1R2, T1R3 and SGLT1 relative gene expression evaluated from the proximal jejunum biopsies. Flavour had no significant effect on the feed intake (p = 0.934), but capsicum increased the average daily weight gain per metabolic body weight (p = 0.049). The T1R3 gene was expressed highest in the mix treatment (1.7; p = 0.005). Collectively, our findings indicate that flavours can be used to motivate feed acceptance and improve the weight gain in lambs. Full article

(This article belongs to the Special Issue Feed Additives, Performance and Welfare in Domestic Animals)

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23 pages, 2451 KiB

Open AccessArticle

A Diet Rich in HUFAs Enhances the Energetic and Immune Response Capacities of Larvae of the Scallop Argopecten purpuratus

by Isis Rojas, Claudia B. Cárcamo, Yohana Defranchi, Katherine Jeno, José Rengel, Michael Araya, María Elena Tarnok, Luis Aguilar, Gonzalo Álvarez, Paulina Schmitt and Katherina Brokordt

Animals 2023, 13(8), 1416; https://doi.org/10.3390/ani13081416 - 20 Apr 2023

Cited by 3 | Viewed by 1976

Abstract

Massive mortalities in farmed larvae of the scallop Argopecten purpuratus have been associated with pathogenic Vibrio outbreaks. An energetic trade-off between development-associated demands and immune capacity has been observed. Given that highly unsaturated fatty acids (HUFAs) are essential nutrients for larval development, we [...] Read more.

Massive mortalities in farmed larvae of the scallop Argopecten purpuratus have been associated with pathogenic Vibrio outbreaks. An energetic trade-off between development-associated demands and immune capacity has been observed. Given that highly unsaturated fatty acids (HUFAs) are essential nutrients for larval development, we evaluated the effect of diets based on microalgae low and high in HUFAs (LH and HH, respectively) on the energetic condition and the immune response of scallop larvae. The results showed that the HH diet increased cellular membrane fluidity in veliger larvae. The routine respiration rate was 64% higher in the HH-fed veligers than in the LH-fed veligers. Additionally, the metabolic capacity tended to be higher in the HH-fed veligers than in the LH-fed veligers after the Vibrio challenge. After the challenge, the HH-fed veligers presented higher transcript induction of ApTLR (immune receptor) and ApGlys (immune effector) genes, and the HH-fed pediveligers presented higher induction of ApLBP/BPI1 (antimicrobial immune effector) gene, than the LH-fed larvae. Furthermore, the HH-fed veligers controlled total Vibrio proliferation (maintaining near basal levels) after the bacterial challenge, while the LH-fed veligers were not able to control this proliferation, which increased three-fold. Finally, the HH-fed larvae showed 20–25% higher growth and survival rates than the LH-fed veligers. Overall, the results indicated that the administration of a HH diet increases cell membrane fluidity and energy metabolic capacity, which in turn enhances immunity and the ability to control Vibrio proliferation. The administration of microalgae high in HUFAs would be a promising strategy for improving scallop larval production efficiency. Full article

(This article belongs to the Special Issue Sustainable Aquaculture Practices: Novel Feeds to Improve Fish Performance, Immunity and Disease Resistance)

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16 pages, 13870 KiB

Open AccessArticle

Chromosomes of Asian Cyprinid Fishes: Genomic Differences in Conserved Karyotypes of ‘Poropuntiinae’ (Teleostei, Cyprinidae)

by Sudarat Khensuwan, Francisco de M. C. Sassi, Renata L. R. Moraes, Sitthisak Jantarat, Kriengkrai Seetapan, Krit Phintong, Weera Thongnetr, Sarawut Kaewsri, Sarun Jumrusthanasan, Weerayuth Supiwong, Petr Rab, Alongklod Tanomtong, Thomas Liehr and Marcelo B. Cioffi

Animals 2023, 13(8), 1415; https://doi.org/10.3390/ani13081415 - 20 Apr 2023

Cited by 3 | Viewed by 2688

Abstract

The representatives of cyprinid lineage ‘Poropuntiinae’ with 16 recognized genera and around 100 species form a significant part of Southeast Asian ichthyofauna. Cytogenetics are valuable when studying fish evolution, especially the dynamics of repetitive DNAs, such as ribosomal DNAs (5S and 18S) and [...] Read more.

The representatives of cyprinid lineage ‘Poropuntiinae’ with 16 recognized genera and around 100 species form a significant part of Southeast Asian ichthyofauna. Cytogenetics are valuable when studying fish evolution, especially the dynamics of repetitive DNAs, such as ribosomal DNAs (5S and 18S) and microsatellites, that can vary between species. Here, karyotypes of seven ‘poropuntiin’ species, namely Cosmochilus harmandi, Cyclocheilichthys apogon, Hypsibarbus malcomi, H. wetmorei, Mystacoleucus chilopterus, M. ectypus, and Puntioplties proctozysron occurring in Thailand were examined using conventional and molecular cytogenetic protocols. Variable numbers of uni- and bi-armed chromosomes indicated widespread chromosome rearrangements with a stable diploid chromosome number (2n) of 50. Examination with fluorescence in situ hybridization using major and minor ribosomal probes showed that Cosmochilus harmandi, Cyclocheilichthys apogon, and Puntioplites proctozystron all had one chromosomal pair with 5S rDNA sites. However, more than two sites were found in Hypsibarbus malcolmi, H. wetmorei, Mystacoleucus chilopterus, and M. ectypus. The number of chromosomes with 18S rDNA sites varied amongst their karyotypes from one to three; additionally, comparative genomic hybridization and microsatellite patterns varied among species. Our results reinforce the trend of chromosomal evolution in cyprinifom fishes, with major chromosomal rearrangements, while conserving their 2n. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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16 pages, 328 KiB

Open AccessReview

The Fibre Requirements of Horses and the Consequences and Causes of Failure to Meet Them

by Colette Ermers, Nerida McGilchrist, Kate Fenner, Bethany Wilson and Paul McGreevy

Animals 2023, 13(8), 1414; https://doi.org/10.3390/ani13081414 - 20 Apr 2023

Cited by 5 | Viewed by 6850

Abstract

Failure to meet the minimum forage requirement of 1.5% of the horse’s bodyweight and the opportunity for foraging for a minimum of 8 h a day (not going without this opportunity longer than four to five consecutive hours) can have both physiological and [...] Read more.

Failure to meet the minimum forage requirement of 1.5% of the horse’s bodyweight and the opportunity for foraging for a minimum of 8 h a day (not going without this opportunity longer than four to five consecutive hours) can have both physiological and behavioural consequences. To provide an energy source for horses, rations often include starch rather than fibre. This can result in health issues related to the gastrointestinal tract (GIT) in the horse. In the stomach, the main concern is equine gastric ulcer syndrome (EGUS) and, more specifically, equine squamous gastric disease (ESGD). Ulcerations are caused either by increasing acidity in the stomach (from starch ingestion and reduced saliva production) or splashing of acidic juices caused by a lack of a forage barrier prior to exercise or prolonged periods without fibrous feed intake, which allows the stomach to collapse and spread acidic gastric fluids into the upper squamous regions of the stomach. In the hindgut, starch that has escaped digestion in the small intestine causes microbial instability and increased production of volatile fatty acids (VFA) and lactic acid. This puts horses at great risk for acidosis and subsequent laminitis. Shifts in the hindgut microbiota will also affect a horse’s behaviour via the gut-brain axis, as well as potentially compromise immune function. Reduced fluid intake caused by reduced saliva production can result in colic. Choosing a fibrous alternative for starch in a high-energy diet greatly reduces the risk of EGUS and acidosis and improves digestion, GIT pH, body condition, behaviour, immune functions, and performance. Providing hay can reduce crib-biting, wood-chewing, coprophagia, the consumption of bedding, aggression, and stress, and subsequently increase social bonding and affiliation with conspecifics. Adequate fibre intake is related to reduced clinical signs of EGUS, reduced reactivity, and better adaptation to weaning. Lignophagia (wood chewing) has also been observed in horses that are foraging, and this is thought to reflect low fibre content in the available forage (for example, early vegetative, lush pasture). Full article

(This article belongs to the Special Issue Forage, Freedom and Friends to Care Health, Nutritional and Ethological Needs of the Equine)

14 pages, 3554 KiB

Open AccessArticle

Effects of Anthropogenic Disturbance of Natural Habitats on the Feeding Ecology of Moorish Geckos

by José Martín, Jesús Ortega, Roberto García-Roa, Gonzalo Rodríguez-Ruiz, Ana Pérez-Cembranos and Valentín Pérez-Mellado

Animals 2023, 13(8), 1413; https://doi.org/10.3390/ani13081413 - 20 Apr 2023

Cited by 1 | Viewed by 1966

Abstract

Urbanization and anthropic influences can drastically modify a natural habitat and transform it into an easily recognizable “urban habitat”. Human activities can also induce less severe modifications of what apparently might still look like natural habitats. Therefore, these subtle alterations may be hidden [...] Read more.

Urbanization and anthropic influences can drastically modify a natural habitat and transform it into an easily recognizable “urban habitat”. Human activities can also induce less severe modifications of what apparently might still look like natural habitats. Therefore, these subtle alterations may be hidden but can still cause important negative effects on plant and animals. In contrast, some species seem able to take advantage of these anthropic alterations. Here, we examined the possible effects of the anthropogenic disturbance of an apparent natural habitat on the feeding ecology and body condition of Moorish geckos, Tarentola mauritanica. For this, we compared microhabitat structure, invertebrate availability, the diet composition (estimated from fecal contents), diet selection patterns and body condition of the two populations of geckos inhabiting two contiguous small islands. These islands have similar environmental characteristics, but highly contrasting differences in urbanization and anthropogenic influence. We found that, although the abundance of potential invertebrate prey was similar on both habitats, the diversity of invertebrate prey was lower in the altered habitat. As a consequence, although composition of the diet of geckos was similar on both islands, the diversity of prey and food niche breadth were lower in the altered habitat, and patterns of diet selection changed. However, these inter-habitat differences did not seem to affect the body size and body condition of geckos. We discuss how flexibility in feeding ecology may allow some species to cope with small anthropic disturbances of the habitat. Full article

(This article belongs to the Section Herpetology)

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12 pages, 1184 KiB

Open AccessArticle

Status and Numbers of the Brown Bear (Ursus arctos L.) in Bulgaria

by Ruslan Serbezov and Nikolai Spassov

Animals 2023, 13(8), 1412; https://doi.org/10.3390/ani13081412 - 20 Apr 2023

Cited by 2 | Viewed by 4229

Abstract

Until recently, the Bulgarian bear population (Ursus arctos L.) was considered one of the significant ones in Europe and one of the few with more than 500 bears. While the numbers of some neighboring populations may be increasing, the Bulgarian population has [...] Read more.

Until recently, the Bulgarian bear population (Ursus arctos L.) was considered one of the significant ones in Europe and one of the few with more than 500 bears. While the numbers of some neighboring populations may be increasing, the Bulgarian population has been on a downward trend since the early 1990s. The probable numbers of the species at the end of the 1980s was about 700–750 individuals. Calculations based on field data from national monitoring and statistical analysis show probable numbers in Bulgaria in 2020 of about 500 individuals (data for the autumn state). This decline is mostly related to poaching due to weaker control activity, the reduction of forest areas and habitat fragmentation. The preservation of the Bulgarian population, which, together with the other Balkan populations and the Apennine bear, has a unique gene pool, is particularly important from the point of view of preserving the biodiversity of the species in Europe. Full article

(This article belongs to the Special Issue Mammal Ecology and Conservation in Southeastern Europe)

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24 pages, 2399 KiB

Open AccessArticle

Ultrasound Morphometry and Mean Echogenicity of Digital Flexor Tendons, Suspensory Ligament, and Accessory Ligament of Digital Deep Flexor Tendon in Gaited Horses

by Jackson Schade, Anderson Fernando de Souza, Lorenzo Costa Vincensi, Thiago Rinaldi Müller and Joandes Henrique Fonteque

Animals 2023, 13(8), 1411; https://doi.org/10.3390/ani13081411 - 20 Apr 2023

Viewed by 3235

Abstract

The objective of this study was to evaluate and compare the sonographic characteristics of the digital flexor tendons and ligaments of the palmar/plantar of the metacarpal and metatarsal regions in gaited horses, as well as to establish normal reference ultrasound values for Mangalarga [...] Read more.

The objective of this study was to evaluate and compare the sonographic characteristics of the digital flexor tendons and ligaments of the palmar/plantar of the metacarpal and metatarsal regions in gaited horses, as well as to establish normal reference ultrasound values for Mangalarga Marchador (MM) and Campeiro breeds. Transverse sonographic images were obtained of 50 adult and healthy horses from the MM (n = 25) and Campeiro (n = 25) breeds. The images were taken in six metacarpal/metatarsal zones, and the following measurements were taken: transverse area, circumference, dorsopalmar/plantar length, lateromedial length, and mean echogenicity. Differences were observed between breeds in the fore- and hindlimbs, and, even when not significant, the Campeiro breed tended to have higher values for most variables and structures. Variations between zones and between structures in the same zone followed a similar trend in both breeds for all variables. In addition, the dimensions and variations between zones and structures were different between the fore- and hindlimbs, highlighting the need for specific values for the digital flexor tendons and ligaments of the metatarsal plantar region. In conclusion, the digital flexor tendons, suspensory ligaments, and accessory ligaments of the digital deep flexor tendon are influenced by the breed factor in gaited horses, and they are different between the fore- and hindlimbs. Full article

(This article belongs to the Special Issue Ultrasonography in Equine Medicine)

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15 pages, 3718 KiB

Open AccessArticle

Luteolin: A Phytochemical to Mitigate S. Typhimurium Flagellin-Induced Inflammation in a Chicken In Vitro Hepatic Model

by Patrik Tráj, Csilla Sebők, Máté Mackei, Ágnes Kemény, Orsolya Farkas, Ákos Kákonyi, László Kovács, Zsuzsanna Neogrády, Ákos Jerzsele and Gábor Mátis

Animals 2023, 13(8), 1410; https://doi.org/10.3390/ani13081410 - 20 Apr 2023

Cited by 5 | Viewed by 1837

Abstract

The use of natural feed supplements is an alternative tool to diminish the damage caused by certain bacteria, improving animal health and productivity. The present research aimed to investigate the proinflammatory effect of flagellin released from the bacterial flagellum of Salmonella enterica serovar [...] Read more.

The use of natural feed supplements is an alternative tool to diminish the damage caused by certain bacteria, improving animal health and productivity. The present research aimed to investigate the proinflammatory effect of flagellin released from the bacterial flagellum of Salmonella enterica serovar Typhimurium and to attenuate the induced inflammation with luteolin as a plant-derived flavonoid on a chicken primary hepatocyte–non-parenchymal cell co-culture. Cells were cultured in a medium supplemented with 250 ng/mL flagellin and 4 or 16 µg/mL luteolin for 24 h. Cellular metabolic activity, lactate dehydrogenase (LDH) activity, interleukin-6, 8, 10 (IL-6, IL-8, IL-10), interferon-α, γ (IFN-α, IFN-γ), hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) concentrations were determined. Flagellin significantly increased the concentration of the proinflammatory cytokine IL-8 and the ratio of IFN-γ/IL-10, while it decreased the level of IL-10, indicating that the model served adequate to study inflammation in vitro. Luteolin treatment at 4 µg/mL did not prove to be cytotoxic, as reflected by metabolic activity and extracellular LDH activity, and significantly reduced the flagellin-triggered IL-8 release of the cultured cells. Further, it had a diminishing effect on the concentration of IFN-α, H₂O₂ and MDA and restored the level of IL-10 and the ratio of IFN-γ/IL-10 when applied in combination with flagellin. These results suggest that luteolin at lower concentrations may protect hepatic cells from an excessive inflammatory response and act as an antioxidant to attenuate oxidative damage. Full article

(This article belongs to the Special Issue Salmonella in Poultry Production: Causes, Impacts, and Solutions)

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8 pages, 575 KiB

Open AccessArticle

Colistin Resistance Genes in Broiler Chickens in Tunisia

by Antonietta Di Francesco, Daniela Salvatore, Sonia Sakhria, Fabrizio Bertelloni, Elena Catelli, Salma Ben Yahia and Aida Tlatli

Animals 2023, 13(8), 1409; https://doi.org/10.3390/ani13081409 - 20 Apr 2023

Cited by 5 | Viewed by 2527

Abstract

Colistin is a polymyxin antibiotic that has been used in veterinary medicine for decades, as a treatment for enterobacterial digestive infections as well as a prophylactic treatment and growth promoter in livestock animals, leading to the emergence and spread of colistin-resistant Gram-negative bacteria [...] Read more.

Colistin is a polymyxin antibiotic that has been used in veterinary medicine for decades, as a treatment for enterobacterial digestive infections as well as a prophylactic treatment and growth promoter in livestock animals, leading to the emergence and spread of colistin-resistant Gram-negative bacteria and to a great public health concern, considering that colistin is one of the last-resort antibiotics against multidrug-resistant deadly infections in clinical practice. Previous studies performed on livestock animals in Tunisia using culture-dependent methods highlighted the presence of colistin-resistant Gram-negative bacteria. In the present survey, DNA extracted from cloacal swabs from 195 broiler chickens from six farms in Tunisia was tested via molecular methods for the ten mobilized colistin resistance (mcr) genes known so far. Of the 195 animals tested, 81 (41.5%) were mcr-1 positive. All the farms tested were positive, with a prevalence ranging from 13% to 93%. These results confirm the spread of colistin resistance in livestock animals in Tunisia and suggest that the investigation of antibiotic resistance genes by culture-independent methods could be a useful means of conducting epidemiological studies on the spread of antimicrobial resistance. Full article

(This article belongs to the Section Animal System and Management)

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17 pages, 1310 KiB

Open AccessArticle

Social Box: A New Housing System Increases Social Interactions among Stallions

by Anja Zollinger, Christa Wyss, Déborah Bardou and Iris Bachmann

Animals 2023, 13(8), 1408; https://doi.org/10.3390/ani13081408 - 20 Apr 2023

Cited by 2 | Viewed by 2964

Abstract

In domestic conditions, adult stallions are mostly housed individually in internal stables to reduce the risk of injuries during social interactions. Social deprivation in horses results in physiological stress and behavioural problems. The aim of this study was to test the “social box” [...] Read more.

In domestic conditions, adult stallions are mostly housed individually in internal stables to reduce the risk of injuries during social interactions. Social deprivation in horses results in physiological stress and behavioural problems. The aim of this study was to test the “social box” (SB), which allows closer physical contact between neighbouring horses. Eight pairs of stallions (n = 16) were filmed over a 24 h period in the SB and in their usual box stables, “conventional boxes” (CB), which strongly restrict tactile contact. The effect of housing in the SB on behaviour and the occurrence and characteristics of injuries was investigated. The total duration of active social interactions was significantly higher in the SB than in the CB (51.1 vs. 4.9 min, p < 0.0001). Positive interactions accounted for about 71% of the total duration of interactions in SB and CB stabling. The stallions interacted significantly more often in the SB than in the CB (113.5 vs. 23.8 social interaction sequences over 24 h, p < 0.0001). No grievous injuries were recorded. The social box appears to be a suitable solution to give adult stallions the possibility of having physical interactions. Therefore, it can be considered a substantial environmental enrichment for singly housed horses. Full article

(This article belongs to the Section Equids)

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12 pages, 1369 KiB

Open AccessArticle

A Comparison of Small Rodent Assemblages after a 20 Year Interval in the Alps

by Giulia Ferrari, Dino Scaravelli, Andrea Mustoni, Marco Armanini, Filippo Zibordi, Olivier Devineau, Francesca Cagnacci, Donato A. Grasso and Federico Ossi

Animals 2023, 13(8), 1407; https://doi.org/10.3390/ani13081407 - 19 Apr 2023

Viewed by 1581

Abstract

Human-induced environmental alterations in the Alps may importantly affect small mammal species, but evidence in this sense is limited. We live-trapped small rodents in the Central-Eastern Italian Alps in three close-by habitat types (rocky scree, alpine grassland, and heath) at 2100 m a.s.l. [...] Read more.

Human-induced environmental alterations in the Alps may importantly affect small mammal species, but evidence in this sense is limited. We live-trapped small rodents in the Central-Eastern Italian Alps in three close-by habitat types (rocky scree, alpine grassland, and heath) at 2100 m a.s.l. during summer-fall, in 1997 and 2016. We compared small rodent assemblages through a Redundancy Detrended Analysis (RDA). In both surveys, we detected two specialist species, i.e., the common vole (Microtus arvalis) and the snow vole (Chionomys nivalis), and, unexpectedly, the forest generalist bank vole (Myodes glareolus). In 1997, grassland was mainly occupied by the common vole, while the bank vole and the snow vole were sympatric in the other habitats. In 2016, the snow vole was detected only in the scree, while other species did not show distribution changes. We discuss a series of hypotheses that might have driven the differences observed across decades, among which is a species-specific response to abiotic and biotic environmental alterations, with the alpine habitat specialist moving out of sub-optimal habitats. We encourage further research on this topic, e.g., via long-term longitudinal studies. Full article

(This article belongs to the Collection Recent Advance in Wildlife Conservation)

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18 pages, 3189 KiB

Open AccessArticle

A Forage Allowance by Forage Type Interaction Impacts the Daily Milk Yield of Early Lactation Dairy Cows

by Adam D. Langworthy, Mark J. Freeman, James L. Hills, David K. McLaren, Richard P. Rawnsley and Keith G. Pembleton

Animals 2023, 13(8), 1406; https://doi.org/10.3390/ani13081406 - 19 Apr 2023

Viewed by 2010

Abstract

We tested for a forage allowance effect on the milk yield of early lactation dairy cow herds grazing swards sown with perennial ryegrass (Lolium perenne L.), white clover (Trifolium repens L.) and plantain (Plantago lanceolata L.) relative to perennial ryegrass [...] Read more.

We tested for a forage allowance effect on the milk yield of early lactation dairy cow herds grazing swards sown with perennial ryegrass (Lolium perenne L.), white clover (Trifolium repens L.) and plantain (Plantago lanceolata L.) relative to perennial ryegrass alone. The examined allowances consisted of offering 12, 14, 16, 18, 20 or 25 kg of dry matter (DM)/cow per day of grazeable herbage, with diverse swards sown as mixtures and spatially adjacent monocultures. After adapting cows to their assigned forage type for 8 days, treatment effects on milk yield and composition, blood metabolites (beta-hydroxybutyrate, non-esterified fatty acids and urea concentrations), body weight change, forage intake and selection differentials for forage species and certain nutrients were monitored over 7 days. We confirmed a forage allowance effect on milk yield improvements in dairy cows grazing diverse swards relative to perennial ryegrass monocultures. Improvements in milk yield were evident at forage allowances of 14 to 20 kg of DM/cow per day, diminishing at the highest allowance of 25 kg of DM/cow per day. Improvements in milk yield for the mixture and spatially adjacent monocultures peaked at forage allowances of 18 and 16 kg of DM/cow per day, equalling increases of 1.3 and 1.2 kg of milk/cow per day, respectively. Full article

(This article belongs to the Section Animal Nutrition)

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11 pages, 3195 KiB

Open AccessArticle

Effects of Eggshell Thickness, Calcium Content, and Number of Pores in Erosion Craters on Hatching Rate of Chinese Alligator Eggs

by Naijing Zhang, Huabin Zhang, Guangwei Fan, Ke Sun, Qingqing Jiang, Zhuowen Lv, Boyang Han, Zhenyuan Nie, Yujie Shao, Yongkang Zhou, Baowei Zhang, Xiaobing Wu and Tao Pan

Animals 2023, 13(8), 1405; https://doi.org/10.3390/ani13081405 - 19 Apr 2023

Cited by 1 | Viewed by 2149

Abstract

The Chinese alligator (Alligator sinensis), found only in a small region in southeastern Anhui Province, is listed as critically endangered (CR) by the International Union for Conservation of Nature (IUCN) due to its current declining population trend. Any abnormalities in the [...] Read more.

The Chinese alligator (Alligator sinensis), found only in a small region in southeastern Anhui Province, is listed as critically endangered (CR) by the International Union for Conservation of Nature (IUCN) due to its current declining population trend. Any abnormalities in the physical properties of an egg can decrease the hatching rate. In particular, eggshells play an essential role in embryo development, motivating us to analyze the microstructures of the eggshells of Chinese alligators. In this study, we categorized the eggshells into two groups, based on the hatching rates, and analyzed the relationship between the eggshell parameters (eggshell thickness, calcium content, and number of pores in erosion craters) and the hatching rate, as well as the relationships between the eggshell parameters. We found that the shells of the eggs with high hatching rates were thicker than those of the eggs with low hatching rates. There were also fewer erosion-crater pores on the surfaces of the eggs with high hatching rates than on the surfaces of the eggs with low hatching rates. Moreover, the shell Ca content was significantly higher in the eggs with high hatching rates than in the eggs with low hatching rates. Cluster modeling indicated that the highest hatching rate occurred when the eggshell thickness was 200–380 µm and there were 1–12 pores. These results suggest that eggs with adequate Ca contents, thicker shells, and less air permeability are more likely to hatch. Furthermore, our findings can inform future studies, which will be vital for the survival of the critically endangered Chinese alligator species. Full article

(This article belongs to the Special Issue Adaptive Responses of Vertebrates to Climate Change)

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20 pages, 1067 KiB

Open AccessArticle

Estimating Excreted Nutrients to Improve Nutrient Management for Grazing System Dairy Farms

by Sharon R. Aarons, Cameron J. P. Gourley and J. Mark Powell

Animals 2023, 13(8), 1404; https://doi.org/10.3390/ani13081404 - 19 Apr 2023

Cited by 1 | Viewed by 1398

Abstract

Improving nutrient management in grazing system dairy farms requires determining nutrient flows through animals, the placement of cows within farms and potential for collection, and the re-use and loss of nutrients. We applied a model incorporating data collected at a range of temporal [...] Read more.

Improving nutrient management in grazing system dairy farms requires determining nutrient flows through animals, the placement of cows within farms and potential for collection, and the re-use and loss of nutrients. We applied a model incorporating data collected at a range of temporal and spatial scales to quantify nutrient excretion in all locations that lactating herds visited on five days over a year on 43 conventional and organic grazing system dairy farms. The calculated nutrient loads excreted by cows in different places were highly skewed; while N, P and K deposited loads were consistent across the year, S, Ca and Mg loads varied between sampling times and seasons. The greatest mean and range in nutrient loads were deposited in paddocks, with the smallest amounts deposited in dairy sheds. All excreted nutrient loads increased with farm and herd sizes and milk production. Mean daily loads of 112, 15, 85, 11, 22 and 13 kg of N, P, K, S, Ca and Mg were deposited by the herds which, when standardised to a 305-day lactation, amounted to 24, 4, 20, 3, 5 and 3 t excreted annually, respectively. In addition to routine manure collection in dairy sheds, ensuring collection and recycling of nutrients excreted on feed pads and holding areas would decrease potential nutrient losses by 29% on average. Non-collected, recycled nutrients were disproportionately returned to paddocks in which cows spent time overnight, and except for S and Ca, nutrient loading rates were greater than rates applied as fertilisers. These data demonstrate the extent of excreted nutrients in grazing dairy systems and indicate the need to account for these nutrients in nutrient management plans for Australian dairy farms. We propose incorporating excretion data in current budgeting tools using data currently collected on most Australian grazing system dairy farms. Full article

(This article belongs to the Section Animal System and Management)

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10 pages, 872 KiB

Open AccessArticle

Molecular Surveillance of Canine Degenerative Myelopathy in Breeding Kennels from Romania

by Vlad Cocostîrc, Anamaria Ioana Paștiu, Anca-Alexandra Doboși, Felix Daniel Lucaci, Maria-Carmen Turcu, Mihai Marian Borzan and Dana Liana Pusta

Animals 2023, 13(8), 1403; https://doi.org/10.3390/ani13081403 - 19 Apr 2023

Cited by 2 | Viewed by 1945

Abstract

Canine degenerative myelopathy (CDM) is a spontaneous neurodegenerative disease. Genetically, CDM is an autosomal recessive disease with incomplete penetrance, most commonly caused by a genetic mutation in exon 2 of gene SOD1 (c.118G > A). This study aimed to determine the mutant allele [...] Read more.

Canine degenerative myelopathy (CDM) is a spontaneous neurodegenerative disease. Genetically, CDM is an autosomal recessive disease with incomplete penetrance, most commonly caused by a genetic mutation in exon 2 of gene SOD1 (c.118G > A). This study aimed to determine the mutant allele frequency associated with CDM in various dog breeds from Romania. Dogs (n = 230) from 26 breeds were included in the study. Genotyping using the PCR-RFLP technique was performed on DNA extracted from oral swabs. The results revealed that 204 dogs were homozygous for the wild-type allele (G/G), 16 were heterozygous (A/G), and 10 were homozygous for the mutant allele (A/A). The mutant allele was identified in Wire Fox Terrier, Romanian Mioritic Shepherd, German Shepherd, Rottweiler, Belgian Shepherd, and Czechoslovakian Wolfdog breeds. The mutant allele frequency (A) within the tested population was 0.0783. The results for Belgian Shepherd, German Shepherd, and Romanian Mioritic Shepherd were in Hardy–Weinberg equilibrium, but a departure was observed for Rottweiler. The current study included a first screening of the Romanian Bucovina Shepherd, Romanian Mioritic Shepherd, and Caucasian Shepherd breeds. Genetic testing for the mutation associated with CDM is important in order to avoid the risk of the emergence of dogs homozygous for the SOD1:c118G > A allele. Full article

(This article belongs to the Section Animal Genetics and Genomics)

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12 pages, 1282 KiB

Open AccessArticle

Characterization of the Germplasm Bank for the Spanish Autochthonous Bull Breed “Asturiana de la Montaña”

by Carolina Tamargo, Amer Salman, Jose Néstor Caamaño, Felipe Martínez-Pastor, Ángel Fernández, Rodrigo Muiño, María Teresa Carbajo and Carlos Olegario Hidalgo

Animals 2023, 13(8), 1402; https://doi.org/10.3390/ani13081402 - 19 Apr 2023

Viewed by 1833

Abstract

Semen cryobanks are critical for preserving autochthonous and rare breeds. Since sperm cryopreservation has been optimized for commercial breeds, non-commercial ones (often endangered) must be characterized to ensure the germplasm’s viability. This study reports an investigation of the “Asturiana de la Montaña” breed [...] Read more.

Semen cryobanks are critical for preserving autochthonous and rare breeds. Since sperm cryopreservation has been optimized for commercial breeds, non-commercial ones (often endangered) must be characterized to ensure the germplasm’s viability. This study reports an investigation of the “Asturiana de la Montaña” breed (AM), a valuable Spanish autochthonous cattle breed adapted to the mountainous Atlantic environment. The survey included cryopreserved semen doses from 40 bulls stored at the Principado de Asturias Germplasm Bank. Data were obtained from the routine fresh semen analysis, CASA (motility), and flow cytometry analyses of fresh and post-thawing semen, and the 56-day non-return-rate (NRR) in heifers and cows (all results as 1st and 3rd quartiles). Fresh samples (artificial vagina) were within the normal range for cattle (4–6 mL, 5–10 × 10⁹/mL; mass motility 5). Post-thawing results showed motility below typical for commercial breeds (total motility 26–43%, progressive 14–28%), with higher values for viability (47–62%). Insemination results showed a good performance for this breed (NRR: 47–56%; higher for heifers). Sperm volume increased with age, with little or no effects on sperm quality. Few associations were found between post-thawing quality or freezability and NRR, LIN being the variable more strongly associated (positively). The AM semen bank shows a good prospect for preserving and disseminating the genetics of this breed. This survey indicates that dedicated research is needed to adapt freezing protocols to this breed, optimizing post-thawing results. Full article

(This article belongs to the Section Animal Reproduction)

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18 pages, 3817 KiB

Open AccessArticle

Pre-Protection and Mechanism of Crude Extracts from Dioscorea alata L. on H₂O₂-Induced IPEC-J2 Cells Oxidative Damage

by Yanhong Yun, Huiyu Shi, Yanyu Wang, Fengyuan Yang, Yuanxin Zhang, Haibo Feng, Junpu Chen and Xuemei Wang

Animals 2023, 13(8), 1401; https://doi.org/10.3390/ani13081401 - 19 Apr 2023

Cited by 2 | Viewed by 1799

Abstract

The purple tubers of Dioscorea alata L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of Dioscorea alata L. and its crude extracts on cells prior to oxidative stress. [...] Read more.

The purple tubers of Dioscorea alata L. have been found to contain a variety of bioactive chemical components, including anthocyanins, which make it significant to investigate the pre-protective effects of Dioscorea alata L. and its crude extracts on cells prior to oxidative stress. To establish a suitable oxidative damage model, an injured model of IPEC-J2 cells was created using H₂O₂ as the oxidant. Specifically, when the concentration of H₂O₂ was 120 μmol/L and the injured time was 8 h, the survival rate of cells decreased to approximately 70%, and the cells exhibited a noticeable oxidative stress reaction. Moreover, the crude extracts of Dioscorea alata L. demonstrated beneficial pre-protective effects on IPEC-J2 cells by increasing the total antioxidant capacity (T-AOC) and catalase (CAT) activities, augmenting the expression of total superoxide dismutase (T-SOD) and its genes, reducing the content of malondialdehyde (MDA) and the activity of glutathione peroxidase (GSH-P_X) and its expression of genes, and promoting the expression of glucose transporter SGLT1 gene while reducing that of GULT2 gene, thereby facilitating the entry of anthocyanins into cells. In addition, the 50 μg/mL crude extracts effectively inhibited the phosphorylation of IκB and the p65 protein, thus reducing cellular oxidative stress. Given these findings, Dioscorea alata L. can be considered a natural antioxidant for practical breeding and production purposes, with an optimal concentration of crude extracts in this experiment being 50 μg/mL. Full article

(This article belongs to the Section Animal Nutrition)

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12 pages, 266 KiB

Open AccessArticle

Evaluating Disease Patterns of Military Working Dogs in the Republic of Korea: A Retrospective Study

by Sanghyeon Park, Gyeonggook Park, Mi-Sun Rieu, Taewoo Kim, Dongwook Kim, Sungin Lee and Gonhyung Kim

Animals 2023, 13(8), 1400; https://doi.org/10.3390/ani13081400 - 19 Apr 2023

Cited by 2 | Viewed by 2175

Abstract

The purpose of this study was to evaluate disease patterns among military working dogs (MWDs) at the Armed Forces Medical Research Institute (AFMRI) to provide basic medical data on Korean MWDs. The medical records of procedures performed at AFMRI between November 2017 and [...] Read more.

The purpose of this study was to evaluate disease patterns among military working dogs (MWDs) at the Armed Forces Medical Research Institute (AFMRI) to provide basic medical data on Korean MWDs. The medical records of procedures performed at AFMRI between November 2017 and March 2021 were reviewed. Physical examination, diagnostic imaging, and laboratory tests were performed according to the status of each dog. A total of 353 MWDs (215 males and 138 females; mean age, 6 ± 3 years) were analyzed in this study. Among Korean MWDs, gastrointestinal (GI) disorders are the most frequently diagnosed, followed by dental and musculoskeletal disorders. Foreign body (FB) ingestion had the highest prevalence of GI disorders, with the most common FB being a leather collar or leash. General and dental surgeries, including removal of gastric FB and tooth extraction, were routinely performed at the AFMRI. Preventative care focusing on dental disease and FB ingestion may be helpful for effective performance and good quality of life in MWDs, with the regular assessment and prevention of environmental factors that may contribute to behavioral problems such as FB ingestion, coprophagy, and anorexia. Full article

(This article belongs to the Section Animal Welfare)

11 pages, 1016 KiB

Open AccessArticle

Proteinuria and Electrophoretic Pattern in Dogs with Comorbidities Associated with Chronic Kidney Disease

by Alicia Pamela Pérez-Sánchez, Sofía Perini-Perera, Javier Del-Angel-Caraza, Israel Alejandro Quijano-Hernández and Sergio Recillas-Morales

Animals 2023, 13(8), 1399; https://doi.org/10.3390/ani13081399 - 19 Apr 2023

Cited by 2 | Viewed by 2559

Abstract

In animals with chronic pathologies, the detection of proteinuria via the proteinuria: creatininuria ratio (UPC) and urinary protein electrophoresis allows for the early diagnosis of chronic kidney disease (CKD). The objective of this work was to identify and determine the magnitude of proteinuria [...] Read more.

In animals with chronic pathologies, the detection of proteinuria via the proteinuria: creatininuria ratio (UPC) and urinary protein electrophoresis allows for the early diagnosis of chronic kidney disease (CKD). The objective of this work was to identify and determine the magnitude of proteinuria and its electrophoretic pattern characterization in dogs with chronic diseases pathophysiologically related to proteinuria. With the studied patients, five groups were formed. The control group (CG) contained non-proteinuric cases. The cases with proteinuria were classified into four groups according to the concurrent disease: chronic inflammatory diseases (IG), neoplasms (NG), heart diseases (HG), and endocrine diseases (EG). For the statistical analysis, descriptive statistics and non-parametric tests were used. Data from 264 dogs were obtained; in the disease groups, proteinuria was observed in more than 30% as the only finding of kidney disease, evidencing a greater risk factor for proteinuria in the HG group (OR 4.047, CI 1.894–8.644, p < 0.0001). In the HG, NG, and EG groups, a higher frequency of glomerular pattern (GEP) related to glomerular hypertension was observed; in the IG, a higher frequency of mixed pattern (MEP) was observed. These findings are secondary to the hyperfiltration process that affects the glomerulus and the renal tubule. Full article

(This article belongs to the Special Issue Advances in Companion Animal Clinical Pathology)

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Animals, Volume 13, Issue 8 (April-2 2023) – 155 articles

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