Animals

2024

Jump to: 2023, 2022, 2021, 2020

17 pages, 321 KiB

Open AccessArticle

Effects of the Marek’s Disease Vaccine on the Performance, Meat Yield, and Incidence of Woody Breast Myopathy in Ross 708 Broilers When Administered Alone or in Conjunction with In ovo and Dietary Supplemental 25-Hydroxycholecalciferol

by Seyed Abolghasem Fatemi, Ayoub Mousstaaid, Christopher J. Williams, Joshua Deines, Sabin Poudel, Ishab Poudel, Elianna Rice Walters, April Waguespack Levy and Edgar David Peebles

Animals 2024, 14(9), 1308; https://doi.org/10.3390/ani14091308 - 26 Apr 2024

Cited by 2 | Viewed by 1510

Abstract

The effects of the Marek’s disease vaccine (MDV) on the live performance, breast meat yield, and incidence of woody breast myopathy (WBM) of Ross 708 broilers were investigated when administered alone or in conjunction with in ovo and dietary supplemental 25-hydroxycholecalciferol (25OHD₃ [...] Read more.

The effects of the Marek’s disease vaccine (MDV) on the live performance, breast meat yield, and incidence of woody breast myopathy (WBM) of Ross 708 broilers were investigated when administered alone or in conjunction with in ovo and dietary supplemental 25-hydroxycholecalciferol (25OHD₃). At 18 d of incubation (doi), four in ovo injection treatments were randomly assigned to live embryonated Ross 708 broiler hatching eggs: (1) non-injected; (2) commercial MDV alone; or MDV containing either (3) 1.2 or (4) 2.4 μg of 25OHD₃. An Inovoject multi-egg injector was used to inject a 50 μL solution volume into each egg. The birds were provided a commercial diet that contained 250 IU of cholecalciferol/kg of feed (control) or a commercial diet that was supplemented with an additional 2760 IU of 25OHD₃/kg of feed (HyD-diet). In the growout period, 14 male broilers were placed in each of 48 floor pens resulting 6 replicated pens per in ovo x dietary treatment combination. Live performance variable were measured at each dietary phases from 0 to 14, 15 to 28, and 29 to 40 d of age (doa). At 14 and 40 doa, pectoralis major (P. major) and pectoralis minor (P. minor) muscles were determined for one bird within each of the six replicate pens. At 41 doa, WBM incidence was determined. No significant main or interaction effects occurred for WBM among the dietary or in ovo injection treatments. However, in response to in ovo 25OHD₃ supplementation, BW and BWG in the 29 to 40 doa period and BWG and FCR in the 0 to 40 doa period improved. In addition, at 40 and 41 doa, breast meat yield increased in response to in ovo and dietary 25OHD₃ supplementation. Future research is needed to determine the possible reasons that may have been involved in the aforementioned improvements. Full article

12 pages, 1580 KiB

Open AccessFeature PaperArticle

Hatchery and Dietary Application of Synbiotics in Broilers: Performance and mRNA Abundance of Ileum Tight Junction Proteins, Nutrient Transporters, and Immune Response Markers

by Mallory B. White, Ali Calik and Rami A. Dalloul

Animals 2024, 14(6), 970; https://doi.org/10.3390/ani14060970 - 20 Mar 2024

Cited by 1 | Viewed by 1503

Abstract

This study investigated the effects of a synbiotic consisting of inulin, Enterococcus faecium, Pediococcus acidilactici, Bifidobacterium animalis, and Lactobacillus reuteri given orally to day (d)-of-hatch (DOH) broiler chicks at the hatchery and in the feed for a 21 d period. [...] Read more.

This study investigated the effects of a synbiotic consisting of inulin, Enterococcus faecium, Pediococcus acidilactici, Bifidobacterium animalis, and Lactobacillus reuteri given orally to day (d)-of-hatch (DOH) broiler chicks at the hatchery and in the feed for a 21 d period. A total of 480 Cobb male broilers were randomly divided into one of four treatments using a 2 × 2 factorial design as follows: (1) control (CTRL) group receiving a gel-only oral application on DOH at the hatchery prior to transport and a non-medicated basal corn/soybean meal starter diet; (2) hatchery synbiotic (HS) receiving an oral gel containing the synbiotic (0.5 mL/bird) at the hatchery and the basal diet; (3) CTRL + dietary synbiotic at 0.5 kg/MT (DS); and (4) HS + dietary synbiotic at 0.5 kg/MT (HSDS). On d 7 and d 21, one bird per pen (eight replicate pens/group) was euthanized, and the ileum was immediately removed for qPCR analysis. Data were subjected to a 2-way ANOVA using GLM procedure (JMP Pro17). A significant diet × hatchery interaction was observed in feed conversion ratio (FCR) from d 14 to d 21 (p = 0.013) where the HS, DS, and HSDS treatments had a significantly lower FCR compared to the CTRL. However, no significant interaction effect was observed for body weight gain (BWG) or FCR during the overall experimental period. No significant interaction was observed in mRNA abundance of the evaluated genes in the ileum on d 7 and d 21. Gel application with the synbiotic significantly reduced sodium-dependent glucose cotransporter 1 (SGLT1) mRNA abundance on d 7 (p = 0.035) in comparison to birds receiving gel alone. Regardless of hatchery application, dietary synbiotic supplementation significantly reduced Toll-like receptor (TLR)2, TLR4, and interleukin (IL)-10 mRNA abundance on d 7 (p = 0.013). In conclusion, these findings showed that hatchery and dietary synbiotic application could have a potential beneficial impact on broiler intestinal immunity by regulating the TLR response, a key element of innate immunity. FCR was improved from d 14 to d 21 after synbiotic application. Future research involving extended grow-out studies with a disease challenge would expand on the implications of an early application of synbiotics. Full article

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10 pages, 279 KiB

Open AccessArticle

The Behavioral and Productive Characteristics of Japanese Quails (Coturnix japonica) Exposed to Different Monochromatic Lighting

by Sezgi Karal, Firdevs Korkmaz Turgud, Doğan Narinç and Ali Aygun

Animals 2024, 14(3), 482; https://doi.org/10.3390/ani14030482 - 1 Feb 2024

Viewed by 1281

Abstract

The purpose of this study is to examine the impact of monochromatic illuminations at wavelengths of white (400–700 nm), green (560 nm), yellow (580 nm), blue (480 nm), and red (660 nm) on the performance and behavioral traits of Japanese quails throughout their [...] Read more.

The purpose of this study is to examine the impact of monochromatic illuminations at wavelengths of white (400–700 nm), green (560 nm), yellow (580 nm), blue (480 nm), and red (660 nm) on the performance and behavioral traits of Japanese quails throughout their fattening period. A total of 300 quails in five lighting experimental groups were housed in their conventional rearing cages. Weekly live weights of quails were measured individually, developmental stability was determined, and behavior and fear tests were performed. The body weight averages of quails exposed to blue, green, and yellow light were determined to be greater than those exposed to white and red light (p < 0.05). In terms of the mature weight parameter and the weight of the inflection point of the Gompertz growth model, the averages of the quails exposed to green and blue monochromatic lighting were higher (p < 0.05). The most negative findings on aggressive behavior were observed in birds exposed to monochromatic yellow and red light. Although the body weight of quails exposed to yellow monochromatic lighting was similar to that of quails exposed to green and blue monochromatic lighting, green and blue monochromatic lighting produced the best results in terms of growth, behavior, and developmental stability characteristics. Consequently, it is believed that consistently using green or blue monochromatic lighting programs when raising Japanese quails may provide economic advantages to the producers. Full article

10 pages, 3367 KiB

Open AccessReview

A Mini-Review of the Main Effects of Essential Oils from Citrus aurantifolia, Ocimum basilicum, and Allium sativum as Safe Antimicrobial Activity in Poultry

by Gabriel da Silva Oliveira, Concepta McManus, Heloisa Alves de Figueiredo Sousa, Pedro Henrique Gomes de Sá Santos and Vinícius Machado dos Santos

Animals 2024, 14(3), 382; https://doi.org/10.3390/ani14030382 - 25 Jan 2024

Cited by 4 | Viewed by 1891

Abstract

Poultry production is accompanied by the use of antimicrobial agents because no production step is free of microorganisms. In the absence of antimicrobial treatments with synthetic drugs, essential oils are among the most cited natural alternatives used to prevent and treat microbial contamination [...] Read more.

Poultry production is accompanied by the use of antimicrobial agents because no production step is free of microorganisms. In the absence of antimicrobial treatments with synthetic drugs, essential oils are among the most cited natural alternatives used to prevent and treat microbial contamination in poultry. Although there are several studies on the antimicrobial properties of essential oils, there is still no review that simultaneously compiles information on the leading antimicrobial role of essential oils from Citrus aurantifolia (CAEO), Ocimum basilicum (OBEO), and Allium sativum (ASEO) in poultry. Awareness of the antimicrobial role of these substances opens the door to encouraging their use in natural antimicrobial protocols and discouraging harmful synthetics in poultry. This review aimed to compile information on applying CAEO, OBEO, and ASEO as antimicrobials in poultry farming. The available literature suggests that these essential oils can proportionately align with the poultry industry’s demands for microbiologically safe food products. Full article

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2023

Jump to: 2024, 2022, 2021, 2020

13 pages, 3954 KiB

Open AccessTechnical Note

A Novel, Efficient Method to Isolate Chicken Primordial Germ Cells from Embryonic Blood Using Cell Culture Inserts

by Xia Zhang, Rui Xian, Yingxiao Fu, Yanyan Dai and Rui Peng

Animals 2023, 13(24), 3805; https://doi.org/10.3390/ani13243805 - 9 Dec 2023

Viewed by 1951

Abstract

Primordial germ cells (PGCs) play a crucial role in preserving poultry genetic resources and conducting transgenic research. A system for the rapid isolation of PGCs from single chicken embryonic blood was established in this paper. We found that PGCs can migrate to the [...] Read more.

Primordial germ cells (PGCs) play a crucial role in preserving poultry genetic resources and conducting transgenic research. A system for the rapid isolation of PGCs from single chicken embryonic blood was established in this paper. We found that PGCs can migrate to the lower layer of chicken embryonic fibroblasts (CEFs) through pores smaller than their diameter, while blood cells cannot, when co-cultured with CEFs of passages two to three. Based on the characteristics of PGCs, we developed a new PGC isolation method (cell culture insert/CEF adhesion method) that utilizes a 3 μm cell culture insert and CEFs of passages two to three. Using this method, approximately 700 PGCs can be isolated from the blood of a single chicken embryo at Hamburger and Hamilton (H&H) stage 17 of development. The separation rate achieved was 87.5%, with a separation purity of 95%. The separation rate of this method was 41.4% higher than the common Percoll density gradient centrifugation method and 33.6% higher than lysis with ACK buffer. PGCs isolated from embryonic blood could proliferate 37-fold within 2 weeks when cultured in a feeder-free culture system. They also continued to express the SSEA-1 and DAZL proteins and retained the ability to migrate in vivo. Overall, PGCs separated using cell culture inserts/CEF adhesion method retain their stem cell characteristics and migration ability. PGCs also exhibit good proliferation efficiency, making them suitable for subsequent transgenic experiments or genetic resource preservation. Full article

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15 pages, 3641 KiB

Open AccessArticle

Chinese Herbal Extracts Mitigate Ammonia Generation in the Cecum of Laying Hens: An In Vitro Study

by Miao Li, Kunxian Feng, Jingyi Chen, Tianxu Liu, Yinbao Wu, Jiandui Mi and Yan Wang

Animals 2023, 13(18), 2969; https://doi.org/10.3390/ani13182969 - 20 Sep 2023

Viewed by 1704

Abstract

The objectives of the study were to screen one or several Chinese herbal extracts with good ammonia emission reduction effects using an in vitro gas production study. The study consisted of a control (without Chinese herbal extract), and 11 experimental groups with added [...] Read more.

The objectives of the study were to screen one or several Chinese herbal extracts with good ammonia emission reduction effects using an in vitro gas production study. The study consisted of a control (without Chinese herbal extract), and 11 experimental groups with added cinnamon extract (CE), Osmanthus extract (OE), tangerine peel extract (TPE), dandelion extract (DE), Coptis chinensis extract (CCE), honeysuckle extract (HE), Pulsatilla root extract (PRE), yucca extract (YE), licorice extract (LE), Ginkgo biloba extract (GBE), or astragalus extract (AE). The results showed that HE, PRE, YE, LE, GBE, and AE significantly reduced ammonia production (p ≤ 0.05). The most significant ammonia inhibition was achieved via AE, resulting in a 26.76% reduction. In all treatments, Chinese herbal extracts had no significant effect on pH, conductivity, or uric acid, urea, and nitrate-nitrogen concentrations (p > 0.05). However, AE significantly reduced urease activity and the relative activity of uricase (p ≤ 0.05). AE significantly increased the relative abundance of Bacteroides and decreased the relative abundance of Clostridium, Desulfovibrio, and Prevotell (p ≤ 0.05). Astragalus extract inhibited ammonia emission from laying hens by changing the gut microbial community structure, reducing the relative abundance of ammonia-producing bacteria, and reducing microorganisms’ uricase and urease activities. Full article

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16 pages, 3107 KiB

Open AccessArticle

Application of Mamdani Fuzzy Inference System in Poultry Weight Estimation

by Erdem Küçüktopçu, Bilal Cemek and Halis Simsek

Animals 2023, 13(15), 2471; https://doi.org/10.3390/ani13152471 - 31 Jul 2023

Cited by 3 | Viewed by 1694

Abstract

Traditional manual weighing systems for birds on poultry farms are time-consuming and may compromise animal welfare. Although automatic weighing systems have been introduced as an alternative, they face limitations in accurately estimating the weight of heavy birds. Therefore, exploring alternative methods that offer [...] Read more.

Traditional manual weighing systems for birds on poultry farms are time-consuming and may compromise animal welfare. Although automatic weighing systems have been introduced as an alternative, they face limitations in accurately estimating the weight of heavy birds. Therefore, exploring alternative methods that offer improved efficiency and precision is necessary. One promising solution lies in the application of AI, which has the potential to revolutionize various aspects of poultry production and management, making it an indispensable tool for the modern poultry industry. This study aimed to develop an AI approach based on the FL model as a viable solution for estimating poultry weight. By incorporating expert knowledge and considering key input variables such as indoor temperature, indoor humidity, and feed consumption, FL-based models were developed with different configurations using Mamdani inferences and evaluated across eight different rearing periods in Samsun, Türkiye. This study’s results demonstrated the effectiveness of FL-based models in estimating poultry weight. The models achieved varying average absolute error values across different age groups of broilers, ranging from 0.02% to 5.81%. These findings suggest that FL-based methods hold promise for accurate and efficient poultry weight estimation. This study opens up avenues for further research in the field, encouraging the exploration of FL-based approaches for improved poultry weight estimation in poultry farming operations. Full article

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12 pages, 264 KiB

Open AccessArticle

Profitability and Cost Analysis for Contract Broiler Production in Turkey

by Suleyman Karaman, Yavuz Taşcıoğlu and Osman Doğan Bulut

Animals 2023, 13(13), 2072; https://doi.org/10.3390/ani13132072 - 22 Jun 2023

Cited by 5 | Viewed by 3941

Abstract

This study uses the data obtained from 63 broiler farms engaged in contract farming in Akhisar, Turkey. The average feed conversion ratio in the broiler farms is 1.75, the average live weight 2.25 kg, and the mean market age 38.9 days. The feed [...] Read more.

This study uses the data obtained from 63 broiler farms engaged in contract farming in Akhisar, Turkey. The average feed conversion ratio in the broiler farms is 1.75, the average live weight 2.25 kg, and the mean market age 38.9 days. The feed conversion rate and the income generated are highly correlated (r = −0.76). The production index is 313.4. According to this production index value, 47.6% of the enterprises are below the average production index. It is highest when the marketing age is greater than 38 days, less than and equal to 40. In other words, it is the optimum market age range where carcass yield is at maximum. The average mortality rate is 4.68%. In 52.4% of the enterprises, the mortality rate is above 5%. There is a low level of correlation between the mortality rate and income (r = −0.26). In broiler farming, mortality rate, and feed conversion ratio are factors that directly affect the success and income of the breeder. In broiler farming, the heating cost has the largest share of the total cost, followed by the litter cost. They are followed by labor, electricity, and pesticide costs. Variable costs account for three-quarters of the total operating costs. The farms have a mean gross value of production of $23.797 per m² and $1.400 per broiler in a breeding period. The profit margin is 0.572 $/kg per broiler. The mean enterprise net income in the breeding period is $9.197 per m² and $0.541 per broiler. These findings suggest that broiler farming is a profitable venture. Full article

17 pages, 662 KiB

Open AccessArticle

Effects of the In ovo Administration of the 6/85 Mycoplasma gallisepticum Vaccine on Layer Chicken Embryo Hatchability and Early Posthatch Performance

by Abdulmohsen H. Alqhtani, Seyed Abolghasem Fatemi, Katie Elaine Collins Elliott, Scott L. Branton, Jeff Dwayne Evans and Edgar David Peebles

Animals 2023, 13(7), 1228; https://doi.org/10.3390/ani13071228 - 31 Mar 2023

Cited by 3 | Viewed by 2418

Abstract

In ovo administration as a possible alternative method of 6/85 MG vaccination was assessed. After 18 days of incubation (doi), the eggs were administered a particular dosage of a live attenuated 6/85 MG vaccine in either the air cell (AC) or amnion (AM). [...] Read more.

In ovo administration as a possible alternative method of 6/85 MG vaccination was assessed. After 18 days of incubation (doi), the eggs were administered a particular dosage of a live attenuated 6/85 MG vaccine in either the air cell (AC) or amnion (AM). The treatments included non-injected eggs and eggs injected into the AC or AM with diluent alone as controls. Treatments also included eggs injected with diluent, which contained 1.73 × 10², or 1.73 × 10⁴ CFU of 6/85 MG. Hatchability of viable injected eggs (HI) and residual embryonic mortality were determined at 22 doi. At hatch and at three weeks posthatch, one hatched chick per treatment replicate was bled and swabbed for the detection of 6/85 MG in the choanal cleft using PCR, serum plate agglutination (SPA), and ELISA methods. The results show that AC in ovo injection of 6/85 MG had no negative impacts on HI or on the live performance of pullets, but that it failed to provide adequate protection (p ≤ 0.0001) in hatchlings or three-week-old pullets. The 1.73 × 10⁴ 6/85 MG CFU dosage injected into the AM decreased the hatchability of injected eggs containing viable embryos (HI; p = 0.009) and was associated with a significant increase in late dead mortality (p = 0.001). Hatchling and three-week-old chick mortalities (p = 0.008) were significantly greater in the 1.73 × 10⁴ CFU-AM treatment group in comparison with the other treatment groups. In addition, the 1.73 and 1.73 × 10² 6/85 MG-AM treatments had no negative effects on the hatching process or on posthatch growth, and the 1.73 × 10² 6/85 MG-AM treatment was more effective in the protection of pullets against MG (p ≤ 0.0001) as compared with the low dosage and non-injected treatment groups. Further research is needed to examine the influence of the 6/85 MG in ovo vaccine on layer immune competence. Full article

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8 pages, 282 KiB

Open AccessTechnical Note

Comparison between Standardized and Modified EZ-DripLoss Determination Methods in Chicken Breast Meat

by Ana Kaić, Zlatko Janječić, Karla Golub and Klemen Potočnik

Animals 2023, 13(6), 1054; https://doi.org/10.3390/ani13061054 - 14 Mar 2023

Cited by 1 | Viewed by 1489

Abstract

The EZ-DripLoss method is relatively new gravimetric method that is used for the determination of excessive drip in meat. The literature reports diversity regarding the use of EZ-DripLoss methodology. In the standardized EZ-DripLoss method, drip loss is calculated as the change in the [...] Read more.

The EZ-DripLoss method is relatively new gravimetric method that is used for the determination of excessive drip in meat. The literature reports diversity regarding the use of EZ-DripLoss methodology. In the standardized EZ-DripLoss method, drip loss is calculated as the change in the container weight, whereas in the modified EZ-DripLoss method, it is calculated as the change in sample weight. This technical note aimed to research the relationship between these two methods on chicken breast meat (40 broilers from the line Ross 308) during the measurement interval of 24, 48, and 72 h. The results showed statistically a significant positive linear increase in drip loss values regardless of the used method during all of the investigated measurement intervals. At 24, 48, and 72 h of storage, the average drip loss in the weighed samples was 0.77, 1.40, and 2.23 percentage points greater than in the not weighed samples (p < 0.0001), respectively. A strong and positive estimate of correlation coefficients between the drip loss of weighed and not weighed samples was found after 24 h (r = 0.95), 48 h (r = 0.92), and 72 h (r = 0.86). This technical report indicates that the used EZ-DripLoss methodology highly influences the drip loss in chicken breast meat and the comparisons of the EZ-DripLoss results should be performed with great caution. Full article

9 pages, 417 KiB

Open AccessArticle

Effects of Dietary Phytosterol Supplementation on the Productive Performance, Egg Quality, Length of Small Intestine, and Tibia Quality in Aged Laying Hens

by Xiangyu Xiao, Yucheng Zhu, Bohua Deng, Jiaojiao Wang, Shiyi Shi, Shaoshuai Wang, Xiaoqing Han, Ling Zhao and Tongxing Song

Animals 2023, 13(4), 662; https://doi.org/10.3390/ani13040662 - 14 Feb 2023

Cited by 3 | Viewed by 2199

Abstract

This study aimed at investigating the effects of phytosterols on the productive performance, egg quality, length of small intestine, and tibia quality in aged laying hens. A total of 960 Dawu Jinfeng commercial laying hens (75 weeks of age) were randomly assigned to [...] Read more.

This study aimed at investigating the effects of phytosterols on the productive performance, egg quality, length of small intestine, and tibia quality in aged laying hens. A total of 960 Dawu Jinfeng commercial laying hens (75 weeks of age) were randomly assigned to three groups. Each group had 16 replicates and every replicate contained four cages (five birds/cage). The control group hens received the basal diet without phytosterols. The hens in the experimental groups received a diet containing phytosterols at concentrations of 20 mg/kg and 40 mg/kg for 7 weeks. The results showed that phytosterols had a linearly increasing effect on egg weight, eggshell surface area, albumen height, and haugh unit at week 5 of experiment (p < 0.05). Supplemental phytosterols linearly and quadratically increased eggshell thickness (p < 0.05). At week 7 of the experiment, dietary supplementation of phytosterols linearly increased egg weight and eggshell weight (p < 0.05). Supplementation of 20 mg/kg, but not 40 mg/kg, phytosterols increased the length of the small intestine. However, dietary phytosterols had no effect on the laying rate, mortality, or liver index (p > 0.1). The results of tibia quality detected by micro-CT also showed no difference in the treatment of phytosterols. Therefore, supplementation with 20 mg/kg phytosterols in the diet improves egg quality and increases the length of small intestine, but has no effects on the quality of the tibia. Full article

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2022

Jump to: 2024, 2023, 2021, 2020

15 pages, 3110 KiB

Open AccessArticle

Optimization of Capture ELISAs for Chicken Cytokines Using Commercially Available Antibodies

by Paulina Krzysica, Loes Verhoog, Sonja de Vries, Coen Smits, Huub F. J. Savelkoul and Edwin Tijhaar

Animals 2022, 12(21), 3040; https://doi.org/10.3390/ani12213040 - 4 Nov 2022

Cited by 6 | Viewed by 3044

Abstract

Cytokines like interferon (IFN)-γ, interleukin (IL)-2, IL-6, IL-10, and IL-12p40 are important biomarkers for characterizing the nature and strength of immune responses. It is important to be able to quantify the cytokines at the protein level in biological samples. Quantification of chicken cytokines [...] Read more.

Cytokines like interferon (IFN)-γ, interleukin (IL)-2, IL-6, IL-10, and IL-12p40 are important biomarkers for characterizing the nature and strength of immune responses. It is important to be able to quantify the cytokines at the protein level in biological samples. Quantification of chicken cytokines is generally performed on the level of messenger RNA (mRNA) by quantitative polymerase chain reaction (qPCR) because very few capture ELISAs for the quantification of chicken cytokine proteins are commercially available. Here, we describe the optimization and validation of capture ELISAs for chicken IL-2, IL-6, IL-10, IL-12p40, and IFN-γ using commercially available antibodies and reagents. First, we determined the optimal concentrations of the antibodies. We then verified the ELISAs’ performance and established that the lower limit of detection (LLOD) for all cytokines was below 32 pg/mL. The ELISAs show the same binding characteristics for recombinant and native cytokines (parallelism was <15.2% CV). Values for inter-assay variation were consistently low and mostly <20% CV. Overall, the optimized capture ELISAs are sensitive (<32 pg/mL) and reliable tools to quantify chicken cytokines. These ELISAs can easily and inexpensively be utilized in any immunological lab and may therefore have wide applicability in immunological research for poultry. Full article

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15 pages, 1307 KiB

Open AccessReview

Effects of Sanitizers on Microbiological Control of Hatching Eggshells and Poultry Health during Embryogenesis and Early Stages after Hatching in the Last Decade

by Gabriel da Silva Oliveira, Concepta McManus, Cristiane Batista Salgado and Vinícius Machado dos Santos

Animals 2022, 12(20), 2826; https://doi.org/10.3390/ani12202826 - 18 Oct 2022

Cited by 15 | Viewed by 5241

Abstract

The sanitization of hatching eggs is the backbone of the hygienic–sanitary management of eggs on farms and extends to the hatchery. Poultry production gains depend on the benefits of sanitizers. Obtaining the maximum yield from incubation free of toxic sanitizers is a trend [...] Read more.

The sanitization of hatching eggs is the backbone of the hygienic–sanitary management of eggs on farms and extends to the hatchery. Poultry production gains depend on the benefits of sanitizers. Obtaining the maximum yield from incubation free of toxic sanitizers is a trend in poultry farming, closely following the concerns imposed through scientific research. The toxic characteristics of formaldehyde, the primary sanitizer for hatching eggs, are disappointing, but it is a cheap, practical and widely used antimicrobial. To overcome this shortcoming, multiple synthetic and natural chemical sanitizers have been, and continue to be, tested on hatching eggs. This review aims to evaluate the effects of different sanitizers on the microbiological quality of hatching eggshells and poultry health during embryogenesis and early stages after hatching. Full article

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13 pages, 1234 KiB

Open AccessArticle

Rapid Oxford Nanopore Technologies MinION Sequencing Workflow for Campylobacter jejuni Identification in Broilers on Site—A Proof-of-Concept Study

by Clara Marin, Francisco Marco-Jiménez, Llucia Martínez-Priego, Griselda De Marco-Romero, Vicente Soriano-Chirona, Laura Lorenzo-Rebenaque and Giuseppe D’Auria

Animals 2022, 12(16), 2065; https://doi.org/10.3390/ani12162065 - 13 Aug 2022

Cited by 6 | Viewed by 3788

Abstract

Campylobacter is recognised as one of the most important foodborne bacteria, with a worldwide health and socioeconomic impact. This bacterium is one of the most important zoonotic players in poultry, where efficient and fast detection methods are required. Current official culture methods for [...] Read more.

Campylobacter is recognised as one of the most important foodborne bacteria, with a worldwide health and socioeconomic impact. This bacterium is one of the most important zoonotic players in poultry, where efficient and fast detection methods are required. Current official culture methods for Campylobacter enumeration in poultry usually include >44 h of culture and >72 h for identification, thus requiring at least five working shifts (ISO/TS 10272-2:2017). Here, we have assembled a portable sequencing kit composed of the Bento Lab and the MinION and developed a workflow for on-site farm use that is able to detect and report the presence of Campylobacter from caecal samples in less than five hours from sampling time, as well as the relationship of Campylobacter with other caecal microbes. Beyond that, our workflow may offer a cost-effective and practical method of microbiologically monitoring poultry at the farm. These results would demonstrate the possibility of carrying out rapid on-site screening to monitor the health status of the poultry farm/flock during the production chain. Full article

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10 pages, 984 KiB

Open AccessCommunication

Effect of Dietary 4-Phenylbuthyric Acid Supplementation on Acute Heat-Stress-Induced Hyperthermia in Broiler Chickens

by Yukako Tokutake, Ryo Takanashi, Motoi Kikusato, Masaaki Toyomizu and Kan Sato

Animals 2022, 12(16), 2056; https://doi.org/10.3390/ani12162056 - 12 Aug 2022

Cited by 6 | Viewed by 2135

Abstract

Hot, humid weather causes heat stress (HS) in broiler chickens, which can lead to high mortality. A recent study found that HS causes endoplasmic reticulum (ER) stress. However, the possible involvement of ER stress in HS-induced physiological alterations in broiler chickens is unclear. [...] Read more.

Hot, humid weather causes heat stress (HS) in broiler chickens, which can lead to high mortality. A recent study found that HS causes endoplasmic reticulum (ER) stress. However, the possible involvement of ER stress in HS-induced physiological alterations in broiler chickens is unclear. This study aimed to evaluate the effect of the dietary supplementation of 4-phenylbutyric acid (4-PBA), an alleviator of ER stress, in acute HS-exposed young broiler chickens. Twenty-eight 14-day-old male broiler chickens (ROSS 308) were divided into two groups and fed either a control diet or a diet containing 4-PBA (5.25 g per kg of diet feed) for 10 days. At 24 days old, each group of chickens was kept in thermoneutral (24 ± 0.5 °C) or acute HS (36 ± 0.5 °C) conditions for 2 h. The results showed that thermoneutral birds supplemented with 4-PBA exhibited no negative effects in terms of broiler body weight gain and tissue weight compared to non-supplemental birds. HS increased body temperature in both the control and 4-PBA groups, but the elevation was significantly lower in the 4-PBA group than in the control group. The plasma non-esterified fatty acid concentration was significantly increased by HS treatment in non-supplemental groups, while the increase was partially attenuated in the 4-PBA group. Moreover, 4-PBA prevented HS-induced gene elevation of the ER stress markers GRP78 and GRP94 in the skeletal muscle. These findings suggest that the 4-PBA effect may be specific to the skeletal muscle in HS-exposed birds and that 4-PBA supplementation attenuated HS-induced muscle ER stress, which could be associated with a supplementation of the body temperature elevation and lipolysis. Full article

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26 pages, 3110 KiB

Open AccessArticle

Physiological Changes in Chicken Embryos Inoculated with Drugs and Viruses Highlight the Need for More Standardization of this Animal Model

by Simone Sommerfeld, Antonio Vicente Mundim, Rogério Reis Silva, Jéssica Santos Queiroz, Maisa Paschoal Rios, Fabiana Oliveira Notário, Alessandra Aparecida Medeiros Ronchi, Marcelo Emílio Beletti, Rodrigo Rodrigues Franco, Foued Salmen Espindola, Luiz Ricardo Goulart and Belchiolina Beatriz Fonseca

Animals 2022, 12(9), 1156; https://doi.org/10.3390/ani12091156 - 29 Apr 2022

Cited by 7 | Viewed by 3225

Abstract

Several studies have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardize the evaluated parameters are needed to better understand and identify the viability of CEs as an [...] Read more.

Several studies have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardize the evaluated parameters are needed to better understand and identify the viability of CEs as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are specific to the model. To achieve this goal, in ovo assays were performed by injecting a virus (Gammacoronavirus) and two drugs (filgrastim and dexamethasone) that cause known changes in adult animals. Although congestion and inflammatory infiltrate were visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not increase granulocytes as we expected. On the other hand, CE weight and red blood cell count were lower with dexamethasone (DX), whereas white blood cell count and biomarkers varied depended on the stage of CE development. Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are reproducible. Full article

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13 pages, 297 KiB

Open AccessArticle

Effects of the In Ovo Vaccination of the ts-11 Strain of Mycoplasma gallisepticum in Layer Embryos and Posthatch Chicks

by Abdulmohsen H. Alqhtani, Seyed A. Fatemi, Katie E. C. Elliott, Scott L. Branton, Jeff D. Evans, Spencer A. Leigh, Patrick D. Gerard and Edgar D. Peebles

Animals 2022, 12(9), 1120; https://doi.org/10.3390/ani12091120 - 27 Apr 2022

Cited by 6 | Viewed by 2523

Abstract

The transmission of the ts-11 strain of Mycoplasma gallisepticum (MG) vaccine (ts-11MGV) between incubated eggs and between hatchlings that was administrated via in ovo injection, and its subsequent effects on their posthatch performance were evaluated. Marek’s disease diluent alone (sham-injected) or containing either [...] Read more.

The transmission of the ts-11 strain of Mycoplasma gallisepticum (MG) vaccine (ts-11MGV) between incubated eggs and between hatchlings that was administrated via in ovo injection, and its subsequent effects on their posthatch performance were evaluated. Marek’s disease diluent alone (sham-injected) or containing either 3.63 × 10¹, 10², 10³, or 10⁴ cfu of ts-11MGV was manually in ovo-injected into the amnion on 18 days of incubation. Egg residue analysis, percentage incubational egg weight loss, hatchability of viable injected eggs, and hatchling body weight (BW) were assessed. Selected hatchlings from each treatment replicate group were swabbed in the choanal cleft for MG DNA detection. Female chick live performance was also assessed through 21 days of posthatch age. Unexposed control sentinel chicks were allocated to each treatment replicate group to assess horizontal transmission. Birds were later swabbed and bled respectively, for detection of MG DNA and IgM production at 21 days posthatch. In all birds, no MG DNA was detected and SPA tests for IgM were negative. Among all variables, only 0 to 21 day BW gain was significantly affected by treatment and was lower in the 3.63 × 10⁴ ts-11 MGV treatment in comparison to all the other treatments. Because ts-11MGV does not exhibit vertical or horizontal transmission capabilities under commercial conditions, it may not be a good candidate for in ovo injection. Full article

8 pages, 230 KiB

Open AccessArticle

Effects of the In Ovo Injection of L-Ascorbic Acid on Broiler Hatching Performance

by Ayoub Mousstaaid, Seyed A. Fatemi, Katie E. C. Elliott, Abdulmohsen H. Alqhtani and Edgar D. Peebles

Animals 2022, 12(8), 1020; https://doi.org/10.3390/ani12081020 - 14 Apr 2022

Cited by 12 | Viewed by 2961

Abstract

Effects of the in ovo injection of various concentrations of L-ascorbic acid (L-AA) on the hatchability and retention levels of L-AA in the serum of broiler embryos were investigated. A total of 960 Ross 708 broilers hatching eggs were randomly divided into four [...] Read more.

Effects of the in ovo injection of various concentrations of L-ascorbic acid (L-AA) on the hatchability and retention levels of L-AA in the serum of broiler embryos were investigated. A total of 960 Ross 708 broilers hatching eggs were randomly divided into four treatment groups: non-injected control, saline-injected control, and saline containing either 12 or 25 mg of L-AA. At 18 days of incubation (doi), injected eggs received a 100 μL volume of sterile saline (0.85%) alone or containing one of the two L-AA levels. Percentage egg weight loss was also determined from 0 to 12 and 12 to 18 doi. Hatch residue analysis was conducted after candling to determine the staging of embryo mortality. At approximately 21 doi, hatchability of live embryonated eggs (HI) and hatchling body weight (BW) were determined. Blood samples were taken at 6 and 24 h after L-AA in ovo injection to determine serum L-AA concentrations. Serum L-AA concentrations, HI, and hatchling BW did not differ among all treatment groups. However, chicks in the non-injected group had a higher (p = 0.05) embryonic mortality at hatch in comparison to those in the 12 mg of L-AA in saline and saline alone treatment groups. These results suggest that the in ovo injection of high levels of L-AA (12 and 25 mg) does not negatively affect HI or serum concentrations of L-AA but has the potential to promote embryonic livability. Further research is needed to determine the retention time of L-AA in the other tissues of broilers, including the cornea of the eye, in response to different levels of supplemental L-AA. Full article

12 pages, 746 KiB

Open AccessArticle

Soybean vs. Pea Bean in the Diet of Medium-Growing Broiler Chickens Raised under Semi-Intensive Conditions of Inner Mediterranean Areas: Growth Performance and Environmental Impact

by Antonella Fatica, Francesco Fantuz, Mengjun Wu, Siria Tavaniello, Giuseppe Maiorano and Elisabetta Salimei

Animals 2022, 12(5), 649; https://doi.org/10.3390/ani12050649 - 3 Mar 2022

Cited by 4 | Viewed by 3673

Abstract

The effects of Glycine max L. (SOY) vs. Pisum sativum L. (PEA) in the diet were investigated on in vivo performance of two medium-growing broiler genotypes and on environmental impact. Sixty Kabir Red Plus and sixty New Red chickens were randomly allocated in [...] Read more.

The effects of Glycine max L. (SOY) vs. Pisum sativum L. (PEA) in the diet were investigated on in vivo performance of two medium-growing broiler genotypes and on environmental impact. Sixty Kabir Red Plus and sixty New Red chickens were randomly allocated in 20 pens (n = 6 birds per pen). Each pen, i.e., experimental unit, received 1.18 kg dry matter (DM) including soybean (3.39%) in SOY, or pea bean (6.78%) in PEA groups. DM intake, DM refusals and bodyweight (BW) were recorded on pen basis, and average daily gain (ADG) and feed conversion rate (FCR) were calculated. Data on in vivo performance were processed by ANOVA General Linear Model followed by Student–Newman–Keuls post hoc test. Greenhouse gas (GHG) emissions were evaluated on a year basis by GLEAM-i software. The diet did not affect (p > 0.05) DM intake and DM refusals, BW, ADG and FCR. Diet x genotypes affected (p < 0.05) DM intake and DM refusals. PEA diet, cheaper than SOY diet, allowed the reduction in total GHG and CO₂, and of meat emission intensity. However, an increase in total CH₄ and N₂O emissions was observed. The replacement of soybean with pea bean can represent a possible management strategy to reach trade-off between good farming practices and environmental protection on small-scale poultry farms of inner Mediterranean areas. Full article

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11 pages, 2010 KiB

Open AccessArticle

Effects of Different Ammonia Concentrations on Pulmonary Microbial Flora, Lung Tissue Mucosal Morphology, Inflammatory Cytokines, and Neurotransmitters of Broilers

by Guangju Wang, Qingxiu Liu, Ying Zhou, Jinghai Feng and Minhong Zhang

Animals 2022, 12(3), 261; https://doi.org/10.3390/ani12030261 - 21 Jan 2022

Cited by 3 | Viewed by 2807

Abstract

Atmospheric ammonia is one of the main environmental stressors affecting the performance of broilers. Previous studies demonstrated that high levels of ammonia altered pulmonary microbiota and induced inflammation. Research into the lung–brain axis has been increasing in recent years. However, the molecular mechanisms [...] Read more.

Atmospheric ammonia is one of the main environmental stressors affecting the performance of broilers. Previous studies demonstrated that high levels of ammonia altered pulmonary microbiota and induced inflammation. Research into the lung–brain axis has been increasing in recent years. However, the molecular mechanisms in pulmonary microbiota altered by ambient ammonia exposure on broilers and the relationship between microflora, inflammation, and neurotransmitters are still unknown. In this study, a total of 264 Arbor Acres commercial meal broilers (21 days old) were divided into 4 treatment groups (0, 15, 25, and 35 ppm group) with 6 replicates of 11 chickens for 21 days. At 7 and 21 D during the trial period, the lung tissue microflora was evaluated by 16S rDNA sequencing, and the content of cytokines (IL-1β, IL-6, and IL-10) and norepinephrine (NE), 5-hydroxytryptamine (5-HT) in lung tissue were measured. Correlation analysis was established among lung tissue microflora diversity, inflammatory cytokines, and neurotransmitters. Results showed that the broilers were not influenced after exposure to 15 ppm ammonia, while underexposure of 25 and 35 ppm ammonia resulted in significant effects on pulmonary microflora, inflammatory cytokines, and neurotransmitters. After exposure to ammonia for 7 and 21 days, both increased the proportion of Proteobacteria phylum and the contents of IL-1β and decreased the content of 5-HT. After exposure to ammonia for 7 days, the increase in Proteobacteria in lung tissue was accompanied by a decrease in 5-HT and an increase in IL-1β. In conclusion, the microflora disturbance caused by the increase in Proteobacteria in lung tissue may be the main cause of the changes in inflammatory cytokines (IL-1β) and neurotransmitters (5-HT), and the damage caused by ammonia to broiler lungs may be mediated by the lung–brain axis. Full article

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2021

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23 pages, 1174 KiB

Open AccessArticle

Telomere Length, Apoptotic, and Inflammatory Genes: Novel Biomarkers of Gastrointestinal Tract Pathology and Meat Quality Traits in Chickens under Chronic Stress (Gallus gallus domesticus)

by Kazeem Ajasa Badmus, Zulkifli Idrus, Goh Yong Meng and Kamalludin Mamat-Hamidi

Animals 2021, 11(11), 3276; https://doi.org/10.3390/ani11113276 - 16 Nov 2021

Cited by 4 | Viewed by 3289

Abstract

This study was designed to examine the potentials of telomere length, mitochondria, and acute phase protein genes as novel biomarkers of gastrointestinal (GI) tract pathologies and meat quality traits. Chickens were fed a diet containing corticosterone (CORT) for 4 weeks and records on [...] Read more.

This study was designed to examine the potentials of telomere length, mitochondria, and acute phase protein genes as novel biomarkers of gastrointestinal (GI) tract pathologies and meat quality traits. Chickens were fed a diet containing corticosterone (CORT) for 4 weeks and records on body weight, telomere length, GI tract and muscle histopathological test, meat quality traits, mitochondria, and acute phase protein genes were obtained at weeks 4 and 6 of age. The body weight of CORT-fed chickens was significantly suppressed (p < 0.05). CORT significantly altered the GI tract and meat quality traits. The interaction effect of CORT and age on body weight, duodenum and ileum crypt depth, pH, and meat color was significant (p < 0.05). CORT significantly (p < 0.05) shortened buffy coat telomere length. UCP3 and COX6A1 were diversely and significantly expressed in the muscle, liver, and heart of the CORT-fed chicken. Significant expression of SAAL1 and CRP in the liver and hypothalamus of the CORT-fed chickens was observed at week 4 and 6. Therefore, telomere lengths, mitochondria, and acute phase protein genes could be used as novel biomarkers for GI tract pathologies and meat quality traits. Full article

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8 pages, 218 KiB

Open AccessArticle

Evaluation of Simulated Shelf-Life Conditions for Food Service Applications on Chicken Tenderloins

by Laura E. Yoder, John G. Rehm, Hunter R. Smith, Daniel A. Tigue, Barney Wilborn, Amit Morey, Christy L. Bratcher, Eugene Blythe and Jason T. Sawyer

Animals 2021, 11(7), 2028; https://doi.org/10.3390/ani11072028 - 7 Jul 2021

Cited by 3 | Viewed by 4105

Abstract

The objective of this study was to validate the shelf-life of marinated and frozen chicken tenderloins. Treatments were randomly assigned to the age of the tenderloins post-harvest, days aged (DA): DA4, DA5, DA6, DA7, and DA8. Microbial analyses were used to analyze the [...] Read more.

The objective of this study was to validate the shelf-life of marinated and frozen chicken tenderloins. Treatments were randomly assigned to the age of the tenderloins post-harvest, days aged (DA): DA4, DA5, DA6, DA7, and DA8. Microbial analyses were used to analyze the growth of aerobic, psychotropic, and lactobacilli bacteria to assess the shelf-life of bulk-packaged chicken tenderloins. Tenderloins were sampled fresh, then vacuum tumbled in a marinade. After marination, the tenderloins were sampled with the remaining tenderloins packaged and frozen (−25 °C). After freezing the chicken tenderloins were slacked in a refrigerated cooler (2.2 °C) for up to 132 h (h) and sampled at 36 h, then every 24 h following. After marination, each treatment significantly (p < 0.05) decreased in aerobic and psychotropic counts except DA4. During slacking, no treatment crossed the threshold of 10⁶ CFU/mL (Log 6) set for this study. Though none crossed the threshold, treatments DA4, DA5, and DA6 had significant (p < 0.05) increases in aerobic bacteria after 7 days of age. The psychotropic bacteria continuously grew at each sampling period, with DA4 and DA5 surpassing the other treatments (p < 0.05) at 108 h and 132 h reaching 105 CFU/mL. Every treatment remained below the spoilage threshold, suggesting that this method of storage is suitable for chicken tenderloin shelf-life. Full article

10 pages, 1456 KiB

Open AccessArticle

Royal Jelly Improves the Morphology of the Reproductive Tract, Internal Egg Quality, and Blood Biochemical Parameters in Laying Hens at the Late Stage of Production

by Mahmoud S. El-Tarabany, Mohamed Abdo Nassan and Ayman S. Salah

Animals 2021, 11(7), 1861; https://doi.org/10.3390/ani11071861 - 23 Jun 2021

Cited by 8 | Viewed by 2819

Abstract

The purpose of this study was to evaluate the effects of royal jelly (RJ) on the morphology of the reproductive tract, egg production, and blood biochemical indices of aged laying hens. In total, 120 Lohman Brown laying hens at week 58 of age [...] Read more.

The purpose of this study was to evaluate the effects of royal jelly (RJ) on the morphology of the reproductive tract, egg production, and blood biochemical indices of aged laying hens. In total, 120 Lohman Brown laying hens at week 58 of age were randomly assigned into three equal groups. Pure RJ was dissolved in distilled water and injected subcutaneously as follows: the first treatment (R₁; 100 mg RJ kg⁻¹); the second treatment (R₂; 200 mg RJ kg⁻¹); the control treatment (CON; 1 mL distilled water). Both RJ-treated groups exhibited a significantly higher number of large yellow follicles (LYFs), small yellow follicles (SYFs), and large white follicles (LWFs) (p ˂ 0.05). Furthermore, RJ treatment significantly increased the diameter and weight of the F1 follicles. However, only the R2 group exhibited significantly greater ovary and uterus weights. RJ treatment did not affect the percentage of oviduct and weight of ovarian stroma. In addition, RJ increased the hen–day egg production rate compared with the CON group; however, only the R₂ group showed greater egg weight (p = 0.032). RJ treatment also improved the albumen height, Haugh units, and yolk index. The administration of RJ significantly decreased the serum glucose, but increased the levels of serum albumen and cholesterol. In conclusion, RJ treatment may improve the morphology of the reproductive tract (including follicular growth and oviduct morphology), egg production rate, and internal egg quality traits of aged laying hens. Full article

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13 pages, 1734 KiB

Open AccessArticle

Oxytetracycline and Florfenicol Concentrations in Food-Additive Premixes Authorised for Broiler Chickens: Assessing Degree of Agreement with Manufacturers Labelling

by Aldo Maddaleno, Matías Maturana, Ekaterina Pokrant, Betty San Martín and Javiera Cornejo

Animals 2021, 11(6), 1797; https://doi.org/10.3390/ani11061797 - 16 Jun 2021

Cited by 1 | Viewed by 4983

Abstract

Antimicrobials premixes are the presentation of choice to administer drugs simultaneously to groups of animals in intensive husbandry systems that require treatment for pathologies of bacterial origin. Among the premixes available for use in poultry, florfenicol and oxytetracycline are commonly administered via food [...] Read more.

Antimicrobials premixes are the presentation of choice to administer drugs simultaneously to groups of animals in intensive husbandry systems that require treatment for pathologies of bacterial origin. Among the premixes available for use in poultry, florfenicol and oxytetracycline are commonly administered via food or water. However, their actual concentration in premixes must meet on-label statements to ensure plasma concentrations reach effective therapeutic levels. Hence, this work was designed for the purpose of verifying whether the concentration of antimicrobial present in five premixes matched their on-label statement. Three oxytetracycline premixes, and two of florfenicol, were analysed using a Xevo TQ-S micro UPLC-MS/MS, and an ABSciex API4000 HPLC-MS/MS, respectively. Analytical methodologies were implemented and validated, showing an R² ≥ 0.99 for the calibration curves. Oxytetracycline was detected in these premixes at concentrations exceeding on-label statements by 13.28%, 21.54%, and 29.68%, whereas florfenicol concentrations detected in premixes were 13.06% and 14.75% lower than expected. Consequently, this work shows that the concentration of active ingredients that are present in commercial formulations effectively differ from those stated on premix labels, and it also highlights how unpredictable their range of variability might be. This must be addressed through solid and updated laws that guarantee an effective pharmaceutical product. Full article

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10 pages, 244 KiB

Open AccessArticle

Evaluation of a Product Derived from Porcine Plasma Protein and a Yeast Product with Similar Biological Activity in Diets of Growing Broilers

by Park Waldroup, Mathew Vaughn and James McNaughton

Animals 2021, 11(6), 1751; https://doi.org/10.3390/ani11061751 - 11 Jun 2021

Cited by 1 | Viewed by 3919

Abstract

The post-hatch period of a broiler is an integral point in their development and for the programming of musculoskeletal and immune cells. Therefore, the efficacy of two bioactive protein products, betaGRO^® (BG) and celluTEIN^® (CT) to impact post-hatch and lifelong development [...] Read more.

The post-hatch period of a broiler is an integral point in their development and for the programming of musculoskeletal and immune cells. Therefore, the efficacy of two bioactive protein products, betaGRO^® (BG) and celluTEIN^® (CT) to impact post-hatch and lifelong development was evaluated. Birds were grown under a low stress environment using clean wood shavings litter and a high stress environment with used litter from a commercial farm that had suffered high mortality. Each additive was fed at 300 g/ton from day 1–14 and 50 g/ton from day 15–42. Growing birds in the high stress environment had a negative impact on performance (p < 0.05); however, addition of either BG or CT successfully mitigated the detriments of the high stress environment (p < 0.05), and in many cases, the performance was equal to or better than the performance of birds on the control diet in the low stress environment. Birds fed BG and CT experienced improvements in 42-day feed conversion, and were more uniform than birds grown consuming the control diet (p < 0.05). Mortality of birds in the high stress environment was reduced by addition of BG or CT (p < 0.05). Addition of BG and CT resulted in improvements in carcass and breast meat yield (p < 0.05). Together, these data suggest that BG and CT can be used interchangeably to improve broiler health and performance. Full article

12 pages, 324 KiB

Open AccessArticle

Assessment of Lippia origanoides Essential Oils in a Salmonella typhimurium, Eimeria maxima, and Clostridium perfringens Challenge Model to Induce Necrotic Enteritis in Broiler Chickens

by Makenly E. Coles, Aaron J. Forga, Roberto Señas-Cuesta, Brittany D. Graham, Callie M. Selby, Álvaro J. Uribe, Blanca C. Martínez, Jaime A. Angel-Isaza, Christine N. Vuong, Xochitl Hernandez-Velasco, Billy M. Hargis and Guillermo Tellez-Isaias

Animals 2021, 11(4), 1111; https://doi.org/10.3390/ani11041111 - 13 Apr 2021

Cited by 12 | Viewed by 3135

Abstract

The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), [...] Read more.

The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), Eimeria maxima (day 18), and C. perfringens (CP, days 22-23); Group 3: dietary supplementation LEO and challenged. On d 25 of age, serum samples were collected to evaluate fluorescein isothiocyanate-dextran (FITC-d), superoxide dismutase (SOD), gamma interferon (IFN-γ), Immunoglobulin A (IgA). Group 3 showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction in NE lesion scores, morbidity and mortality compared with the positive challenge control group (p < 0.05) compared with Group 2. Digested feed supernatant, supplemented with LEO and inoculated with CP, reduced CP burden (p < 0.05). Group 3 also exhibited a significant reduction in FITC-d, IFN-γ and IgA compared with Group 2. However, a significant increase SOD was observed in Group 3 compared with both control groups. Further investigation to compare the effect of LEO and the standard treatment of clostridial NE is required. Full article

7 pages, 784 KiB

Open AccessArticle

EZ-DripLoss Assessment in Chicken Breast Meat Using Different Sample Areas, Fiber Orientation, and Measurement Intervals

by Ana Kaić, Zlatko Janječić, Andrija Žanetić, Nikolina Kelava Ugarković and Klemen Potočnik

Animals 2021, 11(4), 1095; https://doi.org/10.3390/ani11041095 - 12 Apr 2021

Cited by 8 | Viewed by 2371

Abstract

Although the EZ-DripLoss method has been performed in numerous studies, there is a deficiency in our knowledge of the EZ-DripLoss method’s suitability for poultry meat analysis. This study aimed to research the effect of different sample areas (10, 20, and 30 mm; n [...] Read more.

Although the EZ-DripLoss method has been performed in numerous studies, there is a deficiency in our knowledge of the EZ-DripLoss method’s suitability for poultry meat analysis. This study aimed to research the effect of different sample areas (10, 20, and 30 mm; n = 240), and fiber orientations (vertical vs. horizontal) on the EZ-DripLoss in chicken breast meat measured across a period of five days. The influence of sample area on the EZ-DripLoss of chicken breast meat with respect to the fiber orientation and across the five-day measurement interval was significant between 10 and 30 mm samples, and between 20 and 30 mm samples (p < 0.001). The estimated regression coefficient showed that EZ-DripLoss for the samples with horizontal and vertical fiber direction of 10 and 20 mm, significantly increased by 0.04% per hour, while for the samples with vertical fiber direction whose diameter was 30 mm, it significantly increased by 0.06% per hour, and for the horizontal fiber direction, it significantly increased by 0.07% per hour. The samples with vertical fiber orientation had 0.50% greater EZ-DripLoss compared to the samples with horizontal fiber orientation. EZ-DripLoss evaluation in chicken breast should be performed with a sample core diameter of 20 mm, a vertical fiber orientation, and over the course of longer measurement intervals. Full article

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13 pages, 548 KiB

Open AccessCommunication

Evaluation of Three Formulations of Essential Oils in Broiler Chickens under Cyclic Heat Stress

by Jared Ruff, Guillermo Tellez, Jr., Aaron J. Forga, Roberto Señas-Cuesta, Christine N. Vuong, Elizabeth S. Greene, Xochitl Hernandez-Velasco, Álvaro J. Uribe, Blanca C. Martínez, Jaime A. Angel-Isaza, Sami Dridi, Clay J. Maynard, Casey M. Owens, Billy M. Hargis and Guillermo Tellez-Isaias

Animals 2021, 11(4), 1084; https://doi.org/10.3390/ani11041084 - 10 Apr 2021

Cited by 19 | Viewed by 3948

Abstract

The objective of the present research was to assess the dietary supplementation of three formulations of essential oils (EO) in chickens under heat stress (HS). Day-of-hatch Cobb 500 chicks (n = 500) were randomly distributed into four groups: 1. HS control + [...] Read more.

The objective of the present research was to assess the dietary supplementation of three formulations of essential oils (EO) in chickens under heat stress (HS). Day-of-hatch Cobb 500 chicks (n = 500) were randomly distributed into four groups: 1. HS control + control diets; 2. HS + control diets supplemented with 37 ppm EO of Lippia origanoides (LO); 3. HS + control diets supplemented with 45 ppm LO + 45 ppm EO of Rosmarinus officinalis (RO) + 300 ppm red beetroot; 4. HS + 45 ppm LO + 45 ppm RO + 300 ppm natural betaine. Chickens that received the EO showed significant (p < 0.05) improvement on BW, BWG, FI, and FCR compared to control HS chickens. Average body core temperature in group 3 and group 4 was significantly (p < 0.05) reduced compared with the HS control group and group 2. Experimental groups showed a significant reduction in FITC-d at 42 days, a significant increase in SOD at both days but a significant reduction of IFN-γ and IgA compared with HS control (p < 0.05). Bone mineralization was significantly improved by EO treatments (p < 0.05). Together these data suggest that supplemental dietary EO may reduce the harmful effects of HS. Full article

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22 pages, 2263 KiB

Open AccessArticle

Expeller-Pressed Canola (Brassica napus) Meal Modulates the Structure and Function of the Cecal Microbiota, and Alters the Metabolome of the Pancreas, Liver, and Breast Muscle of Broiler Chickens

by G. Douglas Inglis, Benjamin D. Wright, Stephanie A. Sheppard, D. Wade Abbott, Matt A. Oryschak and Tony Montina

Animals 2021, 11(2), 577; https://doi.org/10.3390/ani11020577 - 23 Feb 2021

Cited by 10 | Viewed by 3684

Abstract

The inoculation of one-day-old broiler chicks with the cecal contents from a mature broiler breeder resulted in a highly diverse and uniform cecal bacterial community. CM did not affect feed consumption, weight gain, nor the richness, evenness, or diversity of the cecal bacterial [...] Read more.

The inoculation of one-day-old broiler chicks with the cecal contents from a mature broiler breeder resulted in a highly diverse and uniform cecal bacterial community. CM did not affect feed consumption, weight gain, nor the richness, evenness, or diversity of the cecal bacterial community. However, the structure of the bacterial community was altered in birds fed the CM diet. Although the CM diet was formulated to contain equivalent metabolizable energy to the control diet, it contained more dietary fiber. The abundance of bacterial families, including those that are known to contain species able to metabolize fiber was altered (e.g., bacteria within the families, Methanobacteriaceae, Atopobiaceae, Prevotellaceae, Clostridiales Family XIII, Peptostreptococcaceae, and Succinivibrionaceae), and concentrations of SCFAs were higher in the ceca of birds fed the CM diet. Moreover, concentrations of isoleucine, isobutyrate, glutamate, and 2-oxoglutarate were higher, whereas concentrations of phenyllactic acid, indole, glucose, 3-phenylpropionate, and 2-oxobutyrate were lower in the digesta of chickens that were fed CM. The metabolic profiles of pancreas, liver, and breast muscle tissues of birds fed the CM diet differed from control birds. Metabolites that were associated with energy production, protection against oxidative stress, and pathways of amino acid and glycerophospholipid metabolism had altered concentrations in these tissues. Some of the observed changes in metabolite levels may indicate an increased disease risk in birds fed the CM diet (e.g., pancreatitis), and others suggested that birds mounted metabolic response to offset the adverse impacts of CM (e.g., oxidative stress in the liver). Full article

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8 pages, 651 KiB

Open AccessArticle

Effect of Age on Bone Structure Parameters in Laying Hens

by Masayoshi Yamada, Chongxiao Chen, Toshie Sugiyama and Woo Kyun Kim

Animals 2021, 11(2), 570; https://doi.org/10.3390/ani11020570 - 22 Feb 2021

Cited by 18 | Viewed by 3858

Abstract

Changes in medullary and cortical bone structure with age remain unclear. Twenty Hy-Line W36 hens, 25 or 52 weeks of age, were euthanized, and both tibiae were collected when an egg was present in the magnum. Serial cross sections of the tibiae were [...] Read more.

Changes in medullary and cortical bone structure with age remain unclear. Twenty Hy-Line W36 hens, 25 or 52 weeks of age, were euthanized, and both tibiae were collected when an egg was present in the magnum. Serial cross sections of the tibiae were stained with Alcian blue. The bones were scanned using micro-computed tomography. Trabecular width (Tb.Wi) was significantly higher (p < 0.05) in 25-week-old hens, whereas medullary bone tissue volume (TV) was significantly higher (p < 0.01) in 52-week-old hens. 25-week-old hens had significantly higher (p < 0.01) bone volume fraction (BVF = calcified tissue / TV). Moreover, the cortical bone parameters were significantly higher (TV and bone mineral content (BMC) at p < 0.05, and bone volume (BV) and BVF at p < 0.01) in younger hens. Open porosity and total porosity, which indicate less density, were significantly higher (p < 0.01) in older hens. Older hens showed significantly higher (p < 0.01) tibial diaphysis TV than younger hens. Younger hens had significantly higher (p < 0.01) BV, BVF and bone mineral density (BMD) of the tibial diaphysis. These findings reveal that reductions in medullary bone quality might be associated with age-related low estrogen levels and stimulation of osteoclastic bone resorption by parathyroid hormone. Cortical bone quality decreased with enlargement of the Haversian canals and loss of volume, with a longer egg-laying period leading to osteoporosis. Full article

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16 pages, 459 KiB

Open AccessReview

Role of Dietary Fiber in Poultry Nutrition

by Oscar J. Tejeda and Woo K. Kim

Animals 2021, 11(2), 461; https://doi.org/10.3390/ani11020461 - 9 Feb 2021

Cited by 87 | Viewed by 12430

Abstract

Dietary fiber (DF) is an intrinsic component in plant feedstuffs that has been associated with physiological, structural, and functional changes in the gastrointestinal tract. DF is composed of non-starch polysaccharides (NSP), oligosaccharides, and lignin that scape digestion and enzymatic hydrolysis. In general terms, [...] Read more.

Dietary fiber (DF) is an intrinsic component in plant feedstuffs that has been associated with physiological, structural, and functional changes in the gastrointestinal tract. DF is composed of non-starch polysaccharides (NSP), oligosaccharides, and lignin that scape digestion and enzymatic hydrolysis. In general terms, fiber can be classified as insoluble or soluble based on their solubility in water. Both fiber types have direct nutritional implications in broiler diets. Inclusion of insoluble DF in broiler diets modulates intestinal morphology, digestive organ development, nutrient absorption, growth performance, and intestinal microbiota. Soluble DF is thought to increase intestinal viscosity and is associated with negative changes in intestinal microflora and reduction in nutrient absorption. Nevertheless, there is a group of soluble fibers, integrated by oligosaccharides, that function as prebiotics positively modulating intestinal microbiota. Due to the changes in chemical structure and subsequent variation in functionality, it is a difficult task to assign clear attributes to DF as a whole. Therefore, the following review paper compiles data from research conducted using DF and tries to unify such information into practical decisions to be considered when using DF as a functional nutrient in poultry nutrition. Full article

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15 pages, 1088 KiB

Open AccessArticle

Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat Stress

by Ahmed Mohammed, Manal Mahmoud, Raj Murugesan and Heng-wei Cheng

Animals 2021, 11(2), 427; https://doi.org/10.3390/ani11020427 - 7 Feb 2021

Cited by 28 | Viewed by 4229

Abstract

The aim of this study was to evaluate the effect of a synbiotic containing a probiotic (Enterococcus faecium, Pediococcus acidilactici, Bifidobacterium animalis, and Lactobacillus reuteri) and a prebiotic (fructooligosaccharides) on fear response, memory assessment, and selected stress indicators [...] Read more.

The aim of this study was to evaluate the effect of a synbiotic containing a probiotic (Enterococcus faecium, Pediococcus acidilactici, Bifidobacterium animalis, and Lactobacillus reuteri) and a prebiotic (fructooligosaccharides) on fear response, memory assessment, and selected stress indicators in broilers subjected to heat stress. A total of 360 1-day-old Ross 708 chicks were evenly divided among three treatments: a basal diet mixed with a synbiotic at 0 (G-C), 0.5 (G-0.5X), and 1.0 (G-1.0X) g/kg. After 15 d, the broilers were exposed to 32 °C for 9 h daily until 42 d. The object memory test was conducted at 15 day; touch, novel object, and isolation tests were conducted at 35 day; tonic immobility (TI) took place at 41 day. At 42 day, plasma corticosterone and tryptophan concentrations and heterophile/lymphocyte (H/L) ratios were measured. Compared to controls, synbiotic-fed broilers, regardless of concentration, had a shorter latency to make the first vocalization, with higher vocalization rates during the isolation test (p = 0.001). the G-1.0 group had the lowest H/L ratio (p = 0.001), but higher plasma tryptophan concentrations and a greater number of birds could reach the observer during the touch test (p = 0.001 and 0.043, respectively). The current results indicate that the synbiotic can be used as a growth promoter to reduce the fear response and stress state of heat-stressed broilers. Full article

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17 pages, 3553 KiB

Open AccessArticle

The Effect of Dietary Supplementation with Inorganic or Organic Selenium on the Nutritional Quality and Shelf Life of Goose Meat and Liver

by Zabihollah Nemati, Kazem Alirezalu, Maghsoud Besharati, Benjamin W. B. Holman, Mohammadreza Hajipour and Benjamin M. Bohrer

Animals 2021, 11(2), 261; https://doi.org/10.3390/ani11020261 - 21 Jan 2021

Cited by 9 | Viewed by 3324

Abstract

Ninety-six male goslings were allocated and assigned to treatment using a completely randomized design. Dietary treatments included a basal diet consisting of corn, wheat, and soybean meal with either no additional selenium (CON), 0.3 mg/kg of inorganic selenium (I-Se; sodium selenite), or 0.3 [...] Read more.

Ninety-six male goslings were allocated and assigned to treatment using a completely randomized design. Dietary treatments included a basal diet consisting of corn, wheat, and soybean meal with either no additional selenium (CON), 0.3 mg/kg of inorganic selenium (I-Se; sodium selenite), or 0.3 mg/kg of organic selenium (O-Se; selenium-enriched yeast). After a 56-day feeding period, geese were slaughtered on a common ending day and two geese per pen (n = 24) were used for the analyses conducted in this study. Meat (equal portions of the breast and thigh meat) and liver were collected and evaluated for proximate composition, fatty acid profile, pH, phenolic content, thiobarbituric acid reactive substances (TBARS), and total volatile basic nitrogen (TVB-N) over a 9-day storage period at 4 °C. The meat and liver samples from geese supplemented I-Se or O-Se had greater (p < 0.01) lipid content compared with geese not supplemented with additional selenium. At the conclusion of the 9-day storage period, meat and liver samples from geese supplemented I-Se or O-Se had lower (p < 0.05) pH values, greater (p < 0.05) phenolic content, lower (p < 0.05) TBARS values, and lower (p < 0.05) TVB-N compared with geese not supplemented with additional selenium (CON). Full article

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2020

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21 pages, 1444 KiB

Open AccessReview

Dietary Application of Tannins as a Potential Mitigation Strategy for Current Challenges in Poultry Production: A Review

by Janghan Choi and Woo Kyun Kim

Animals 2020, 10(12), 2389; https://doi.org/10.3390/ani10122389 - 14 Dec 2020

Cited by 45 | Viewed by 8593

Abstract

The poultry industry has an important role in producing sources of protein for the world, and the size of global poultry production continues to increase annually. However, the poultry industry is confronting diverse challenges including bacterial infection (salmonellosis), coccidiosis, oxidative stress, including that [...] Read more.

The poultry industry has an important role in producing sources of protein for the world, and the size of global poultry production continues to increase annually. However, the poultry industry is confronting diverse challenges including bacterial infection (salmonellosis), coccidiosis, oxidative stress, including that caused by heat stress, welfare issues such as food pad dermatitis (FPD) and nitrogen and greenhouse gasses emissions that cumulatively cause food safety issues, reduce the efficacy of poultry production, impair animal welfare, and induce environmental issues. Furthermore, restrictions on the use of AGP have exacerbated several of these negative effects. Tannins, polyphenolic compounds that possess a protein precipitation capacity, have been considered as antinutritional factors in the past because high dosages of tannins can decrease feed intake and negatively affect nutrient digestibility and absorption. However, tannins have been shown to have antimicrobial, antioxidant and anti-inflammatory properties, and as such, have gained interest as promising bioactive compounds to help alleviate the challenges of AGP removal in the poultry industry. In addition, the beneficial effects of tannins can be enhanced by several strategies including heat processing, combining tannins with other bioactive compounds, and encapsulation. As a result, supplementation of tannins alone or in conjunction with the above strategies could be an effective approach to decrease the need of AGP and otherwise improve poultry production efficiency. Full article

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14 pages, 1674 KiB

Open AccessArticle

GC-TOF-MS-Based Metabolomics Analyses of Liver and Intestinal Contents in the Overfed vs. Normally-Fed Geese

by Minmeng Zhao, Ya Xing, Lidong Liu, Xiang Fan, Long Liu, Tuoyu Geng and Daoqing Gong

Animals 2020, 10(12), 2375; https://doi.org/10.3390/ani10122375 - 11 Dec 2020

Cited by 10 | Viewed by 2828

Abstract

No overt pathological symptoms are observed in the goose liver with severe steatosis, suggesting that geese may host unique protective mechanisms. Gas chromatography time-of-flight mass spectrometry-based metabolomics analyses of liver and intestinal contents in overfed vs. normally fed geese (26 geese in each [...] Read more.

No overt pathological symptoms are observed in the goose liver with severe steatosis, suggesting that geese may host unique protective mechanisms. Gas chromatography time-of-flight mass spectrometry-based metabolomics analyses of liver and intestinal contents in overfed vs. normally fed geese (26 geese in each treatment) were investigated. We found that overfeeding significantly changed the metabolic profiles of liver and intestinal contents. The differential metabolites mainly belong to fatty acids, amino acids, organic acids, and amines. The differential metabolites were involved in glycolysis/gluconeogenesis, glycerolipid metabolism, the pentose phosphate pathway, fatty acid degradation, the sphingolipid signaling pathway, and the biosynthesis of unsaturated fatty acids. Moreover, we determined the biological effects of arachidonic acid (ARA) and tetrahydrocorticosterone (TD) in goose primary hepatocytes and intestinal cells. Data showed that the mRNA expression of arachidonate 5-lipoxygenase (ALOX5) in goose primary intestinal cells was significantly induced by 0.50 mM ARA treatment. Cytochrome P-450 27A1 (CYP27A1) mRNA expression was significantly inhibited in goose primary hepatocytes by 1 µM TD treatment. In conclusion, the formation of goose fatty liver is accompanied by significant changes in the metabolic profiles of liver and intestinal contents, and the changes are closely related to the metabolisms of glucose and fatty acids, oxidative stress, and inflammatory reactions. Full article

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13 pages, 1298 KiB

Open AccessArticle

Effects of Different Laying Hen Species on Odour Emissions

by Dongdong Lu, Jiandui Mi, Yinbao Wu, Juanboo Liang, Xindi Liao and Yan Wang

Animals 2020, 10(11), 2172; https://doi.org/10.3390/ani10112172 - 21 Nov 2020

Cited by 5 | Viewed by 2804

Abstract

Odour is one of the main environmental concerns in the laying hen industry and may also influence animal health and production performance. Previous studies showed that odours from the laying hen body are primarily produced from the microbial fermentation (breakdown) of organic materials [...] Read more.

Odour is one of the main environmental concerns in the laying hen industry and may also influence animal health and production performance. Previous studies showed that odours from the laying hen body are primarily produced from the microbial fermentation (breakdown) of organic materials in the caecum, and different laying hen species may have different odour production potentials. This study was conducted to evaluate the emissions of two primary odorous gases, ammonia (NH₃) and hydrogen sulphide (H₂S), from six different laying hen species (Hyline, Lohmann, Nongda, Jingfen, Xinghua and Zhusi). An in vitro fermentation technique was adopted in this study, which has been reported to be an appropriate method for simulating gas production from the microbial fermentation of organic materials in the caecum. The results of this study show that Jingfen produced the greatest volume of gas after 12 h of fermentation (p < 0.05). Hyline had the highest, while Lohmann had the lowest, total NH₃ emissions (p < 0.05). The total H₂S emissions of Zhusi and Hyline were higher than those of Lohmann, Jingfen and Xinghua (p < 0.05), while Xinghua exhibited the lowest total H₂S emissions (p < 0.05). Of the six laying hen species, Xinghua was identified as the best species because it produced the lowest total amount of NH₃ + H₂S (39.94 µg). The results for the biochemical indicators showed that the concentration of volatile fatty acids (VFAs) from Zhusi was higher than that for the other five species, while the pH in Zhusi was lower (p < 0.01), and the concentrations of ammonium nitrogen (NH⁴⁺), uric acid and urea in Xinghua were lower than those in the other species (p < 0.01). Hyline had the highest change in SO₄²⁻ concentration during the fermentation processes (p < 0.05). In addition, the results of the correlation analysis suggested that NH₃ emission is positively related to urease activities but is not significantly related to the ureC gene number. Furthermore, H₂S emission was observed to be significantly related to the reduction of SO₄²⁻ but showed no connection with the aprA gene number. Overall, our findings provide a reference for future feeding programmes attempting to reduce odour pollution in the laying hen industry. Full article

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15 pages, 682 KiB

Open AccessReview

Secondary Functions of Arginine and Sulfur Amino Acids in Poultry Health: Review

by Fernanda Lima de Souza Castro and Woo K. Kim

Animals 2020, 10(11), 2106; https://doi.org/10.3390/ani10112106 - 13 Nov 2020

Cited by 38 | Viewed by 6397

Abstract

Amino acids such as arginine, methionine, and cysteine are the precursors of essential molecules that regulate growth and health, being classified as functional amino acids. This review describes the metabolism of arginine and the sulfur amino acids and how they modulate, directly or [...] Read more.

Amino acids such as arginine, methionine, and cysteine are the precursors of essential molecules that regulate growth and health, being classified as functional amino acids. This review describes the metabolism of arginine and the sulfur amino acids and how they modulate, directly or indirectly, different tissues. Emphasis is placed on their effects in supporting health during challenging conditions, such as heat stress and Eimeria infection. The use of arginine has been shown to reduce abdominal fat pad in ducks and increase lean tissue and bone mineral density in broilers. Additionally, the sulfur amino acids have been shown to improve bone development and are beneficial during heat stress. The use of L-methionine increased the cortical and trabecular bone mineral densities, in laying hens. Moreover, the dietary inclusion of these amino acids could reduce the damage caused by Eimeria spp. infection by regulating the antioxidant system and cell repair. Understanding how these amino acids can mitigate stressful conditions may provide us novel insights of their use as nutritional strategies to modulate the health status of chickens. Full article

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19 pages, 1307 KiB

Open AccessReview

Understanding of Colistin Usage in Food Animals and Available Detection Techniques: A Review

by Harsh Kumar, Bing-Huei Chen, Kamil Kuca, Eugenie Nepovimova, Ankur Kaushal, Rupak Nagraik, Shashi Kant Bhatia, Daljeet Singh Dhanjal, Vinod Kumar, Anil Kumar, Navneet Kumar Upadhyay, Rachna Verma and Dinesh Kumar

Animals 2020, 10(10), 1892; https://doi.org/10.3390/ani10101892 - 16 Oct 2020

Cited by 32 | Viewed by 5824

Abstract

Progress in the medical profession is determined by the achievements and effectiveness of new antibiotics in the treatment of microbial infections. However, the development of multiple-drug resistance in numerous bacteria, especially Gram-negative bacteria, has limited the treatment options. Due to this resistance, the [...] Read more.

Progress in the medical profession is determined by the achievements and effectiveness of new antibiotics in the treatment of microbial infections. However, the development of multiple-drug resistance in numerous bacteria, especially Gram-negative bacteria, has limited the treatment options. Due to this resistance, the resurgence of cyclic polypeptide drugs like colistin remains the only option. The drug, colistin, is a well-known growth inhibitor of Gram-negative bacteria like Acinetobacter baumanni, Enterobacter cloacae, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Technological advancements have uncovered the role of the mcr-1(mobilized colistin resistance) gene, which is responsible for the development of resistance in Gram-negative bacteria, which make them distinct from other bacteria without this gene. Additionally, food animals have been determined to be the reservoir for colistin resistance microbes, from which they spread to other hosts. Due to the adverse effects of colistin, many developed countries have prohibited its usage in animal foods, but developing countries are still using colistin in animal food production, thereby imposing a major risk to the public health. Therefore, there is a need for implementation of sustainable measures in livestock farms to prevent microbial infection. This review highlights the negative effects (increased resistance) of colistin consumption and emphasizes the different approaches used for detecting colistin in animal-based foods as well as the challenges associated with its detection. Full article

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11 pages, 429 KiB

Open AccessArticle

Effect of Pre-Blended Phosphates on the Freezing Quality Characteristics of Ground Woody Breast Meat Compared to Normal Meat

by Laura J. Garner, Lasheda Brooks, Lindsey F. Spencer, John Rehm, Jasmine Kataria and Amit Morey

Animals 2020, 10(10), 1880; https://doi.org/10.3390/ani10101880 - 15 Oct 2020

Cited by 5 | Viewed by 2720

Abstract

Woody breast (WB) myopathy affected meat has a tough texture, higher cook loss, and decreased water holding capacity (WHC), and thus lower consumer acceptability. The WB meat can be ground and further converted into further processed products or frozen, stored, and shipped to [...] Read more.

Woody breast (WB) myopathy affected meat has a tough texture, higher cook loss, and decreased water holding capacity (WHC), and thus lower consumer acceptability. The WB meat can be ground and further converted into further processed products or frozen, stored, and shipped to further processors. Freezing and thawing of ground WB meat may further affect the quality of WB meat products. Hence, research is required to determine the effect of pre-blended phosphates on the quality of ground WB meat as well as its cryoprotective effect during frozen storage. The objective of this experiment was to investigate the effect of pre-blended phosphate levels on meat quality in WB and normal breast (NB) fillets before and after freezing. NB fillets and severely affected WB fillets were procured from a local commercial processor. The meat was separated into various treatment groups according to the sodium tripolyphosphate (STPP) inclusion levels (0, 0.25, and 0.5% w/w). The meat was ground with respective phosphate treatments and subdivided into vacuum-sealed bags (n = 240; 1 kg each). Half of the bags (n = 120) from each treatment were taken for meat quality analysis, while the other bags were placed in a freezer (−18 °C) for 6 days. Fresh samples were analyzed within 6–8 h while the frozen samples were thawed for 18 h at 4°C prior to analysis. Samples (n = 10) were analyzed for gel strength, pH, color (L* a* b*), proximate composition, and randomly selected samples (n = 5) were analyzed for aerobic plate count (APC). Experiments were repeated in two separate replications and the data was analyzed using the Proc Glimmix model procedure in SAS (v. 9.4) (Cary, NC, USA) with LSMeans Separation at p ≤ 0.05. The gel strength (g) of the fresh ground NB meat (883.7 g) was higher than the gel strength of woody meat (720.8 g) with 0% phosphate (p ≤ 0.05). Addition of phosphate (0.25 and 0.5%) significantly increased the gel strength of fresh woody meat but it was significantly lower than NB meat added with 0.25 and 0.5% phosphate treatment. After freezing, ground NB meat samples with 0.25 and 0.5% phosphate had higher gel strength compared to fresh and frozen ground WB meat (p ≤ 0.05). Pre-blended STPP raised the pH in all treatments (p < 0.05). Treatments did not have any clear impact on APC of ground WB or NB meat. Addition of pre-blended sodium tripolyphosphate increases the functionality of fresh and frozen ground WB meat, as well as NB meat. Full article

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6 pages, 208 KiB

Open AccessCommunication

Impact of Rotten Eggs on Hatchery Performances: A Multicentric Study

by Giovanni Franzo, Wessel Swart, Miren Arbe Ugalde, Higor Cotta, Mathilde Lecoupeur, William Boyer, Kostas Koutoulis and Mattia Cecchinato

Animals 2020, 10(10), 1725; https://doi.org/10.3390/ani10101725 - 23 Sep 2020

Cited by 1 | Viewed by 3416

Abstract

Day-old chick quality is an essential element for the overall profitability of the broiler productive cycle and has been associated with the growth performance and feed conversion rate. An effect on the development of the immune system was also reported, which could likely [...] Read more.

Day-old chick quality is an essential element for the overall profitability of the broiler productive cycle and has been associated with the growth performance and feed conversion rate. An effect on the development of the immune system was also reported, which could likely account for reduced susceptibility to infectious diseases and improved animal welfare parameters. Besides direct cost reduction, lower antimicrobial use and improved animal welfare are crucial in the directive of European Union legislation and are at the forefront of customer choices. Several factors contribute to determining the chick quality. Breeder flocks genetics, health, and management affect the egg features, quality, and bacterial load. However, hatchery practices play a pivotal role, since adequate hygiene and handling are fundamental in reducing egg contamination and cross-contamination. The presence of rotten eggs is often regarded as a major risk, since the internal bacterial load can contaminate the needle used for in-ovo vaccination, the nearby eggs, and the whole incubator/hatching room when broken. In the present multicentric study, representative of 40 hatcheries located in 11 European countries, a remarkable impact of the rotten egg percentage on the hatchery productive parameters, representative of the hatchability, embryo mortality, and level of contamination, was demonstrated. Efficient rotten egg removal and the application of appropriate detection and removal tools should thus provide remarkable benefits for hatchery performance and indirectly for downstream poultry production. Full article

18 pages, 428 KiB

Open AccessArticle

Interactions between Egg Storage Duration and Breeder Age on Selected Egg Quality, Hatching Results, and Chicken Quality

by Hedia Nasri, Henry van den Brand, Taha Najar and Moncef Bouzouaia

Animals 2020, 10(10), 1719; https://doi.org/10.3390/ani10101719 - 23 Sep 2020

Cited by 18 | Viewed by 5900

Abstract

Egg storage duration and breeder age are probably interacting to influence egg quality, hatchability, and hatchling quality. To evaluate this interaction, the impact of breeder age (31, 42, 66 weeks) and storage duration (2, 5, 12, 19 days) was investigated on broiler breeder [...] Read more.

Egg storage duration and breeder age are probably interacting to influence egg quality, hatchability, and hatchling quality. To evaluate this interaction, the impact of breeder age (31, 42, 66 weeks) and storage duration (2, 5, 12, 19 days) was investigated on broiler breeder eggs (Arbor Acres). Thick albumen diameter and pH increased, and yolk dry matter decreased between 2 and 19 days of storage. With the increase of breeder age from 31 to 66 weeks, albumen height, percentage and dry matter and shell percentage decreased and the egg weight and yolk percentage, dry matter and diameter increased. Prolonged egg storage increased the yolk pH in all breeder ages, but earlier and steeper in the oldest breeders. Prolonged egg storage resulted in a lower hatchability of set and fertile eggs due to a higher percentage of embryonic mortality. Early mortality increased earlier and steeper with prolonged egg storage in the oldest compared to younger breeders. Between 5 and 19 days of storage, yolk free body mass, liver and proventriculus + gizzard percentages decreased, as well as hatchling length and yolk efficiency (yolk absorption per initial yolk weight). The latter effects were most pronounced in the younger than in the older breeders. Therefore, eggs are preferably stored shorter than 7 d, but if long storage (≥12 days) cannot be avoided, we recommend to store eggs of older breeders when egg quality and hatchability are most important. In case hatchling quality is most important, it would be better to store eggs of younger breeders (31 weeks) for a prolonged period. Full article

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16 pages, 1316 KiB

Open AccessFeature PaperArticle

Genotyping of Infectious Laryngotracheitis Virus (ILTV) Isolates from Western Canadian Provinces of Alberta and British Columbia Based on Partial Open Reading Frame (ORF) a and b

by Catalina Barboza-Solis, Ana Perez Contreras, Victor A. Palomino-Tapia, Tomy Joseph, Robin King, Madhu Ravi, Delores Peters, Kevin Fonseca, Carl A. Gagnon, Frank van der Meer and Mohamed Faizal Abdul-Careem

Animals 2020, 10(9), 1634; https://doi.org/10.3390/ani10091634 - 11 Sep 2020

Cited by 7 | Viewed by 3614

Abstract

Infectious laryngotracheitis virus (ILTV) causes an acute upper respiratory disease in chickens called infectious laryngotracheitis (ILT). Live attenuated vaccines are effective in disease control; however, they have residual virulence, which makes them able to replicate, cause disease and revert to the original virulent [...] Read more.

Infectious laryngotracheitis virus (ILTV) causes an acute upper respiratory disease in chickens called infectious laryngotracheitis (ILT). Live attenuated vaccines are effective in disease control; however, they have residual virulence, which makes them able to replicate, cause disease and revert to the original virulent form. Information is scarce on the molecular nature of ILTV that is linked to ILT in Canada. This study aims to determine whether isolates originating from ILT cases in Western Canada are a wild type or vaccine origin. Samples submitted for the diagnosis of ILT between 2009–2018 were obtained from Alberta (AB, n = 46) and British Columbia (BC, n = 9). For genotyping, a Sanger sequencing of open reading frame (ORF) a and b was used. A total of 27 from AB, and 5 from BC samples yielded a fragment of 1751 base pairs (bp). Three of the BC samples classified as group IV (CEO vaccine strains) and 2 as group V (CEO revertant). Of the AB samples, 22 samples clustered with group V, 3 with group VI (wild type), and 2 with group VII, VIII, and IX (wild type). Overall, 17 non-synonymous single nucleotide polymorphisms (SNPs) were detected. Further studies are underway to ascertain the virulence and transmission potential of these isolates. Full article

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6 pages, 965 KiB

Open AccessCommunication

Studying the Effects of Collagenase (Type 1) on the Collagen in Woody Breast Meat

by Amit Morey, Meredith Lane Johnson, Jasmine Kataria and John Michael Gonzalez

Animals 2020, 10(9), 1602; https://doi.org/10.3390/ani10091602 - 9 Sep 2020

Cited by 1 | Viewed by 2748

Abstract

Abnormal collagen infiltration in the Pectoralis major, breast muscle, of fast-growing big broilers has led to woody breast (WB) myopathy resulting in meat quality issues. Mechanisms to degrade the collagen were investigated to potentially improve WB texture. Freshly deboned WB fillets ( [...] Read more.

Abnormal collagen infiltration in the Pectoralis major, breast muscle, of fast-growing big broilers has led to woody breast (WB) myopathy resulting in meat quality issues. Mechanisms to degrade the collagen were investigated to potentially improve WB texture. Freshly deboned WB fillets (n = 5 per trial; 3 trials) were ground and divided in to 25 g portions. Aqueous collagenase Type I solution (1 mL) from concentrations of 2.5, 5, and 10 mg/mL were incorporated in ground WB samples (n = 3 samples/treatment × 3 trials). Ground WB with 1 mL water acted as a control. All the samples were placed at 4 °C for 24 h and frozen at −80 °C. Control samples without any treatment or water addition (n = 3/trial) were frozen immediately upon grinding. Data collected on total (TC), soluble (SC), and insoluble collagen (IC) content was analyzed using one-way ANOVA with Tukey’s honestly significant difference (HSD) (p ≤ 0.05). Fresh WB fillets had TC, SC, and IC content of 19.5, 4.9, and 14.6 mg/g, respectively. The addition of collagenase decreased (p ≤ 0.05) the IC to 5.8 mg/g in the 10 mg/mL treatment after 24 h. Converting IC to SC using collagenase can potentially help the poultry industry to reduce WB toughness. Full article

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17 pages, 3347 KiB

Open AccessArticle

Effects of RAC1 on Proliferation of Hen Ovarian Prehierarchical Follicle Granulosa Cells

by Thobela Louis Tyasi, Xue Sun, Xuesong Shan, Simushi Liswaniso, Ignatius Musenge Chimbaka, Ning Qin and Rifu Xu

Animals 2020, 10(9), 1589; https://doi.org/10.3390/ani10091589 - 6 Sep 2020

Cited by 10 | Viewed by 2956

Abstract

RAC1 belongs to the small G protein Rho subfamily and is implicated in regulating gene expression, cell proliferation and differentiation in mammals and humans; nevertheless, the function of RAC1 in growth and development of hen ovarian follicles is still unclear. This study sought [...] Read more.

RAC1 belongs to the small G protein Rho subfamily and is implicated in regulating gene expression, cell proliferation and differentiation in mammals and humans; nevertheless, the function of RAC1 in growth and development of hen ovarian follicles is still unclear. This study sought to understand the biological effects of RAC1 on granulosa cell (GC) proliferation and differentiation of hen ovarian prehierarchical follicles. Firstly, our results showed expression levels of RAC1 mRNA in the follicles with diameters of 7.0–8.0 mm, 6.0–6.9 mm and 1.0–3.9 mm were greater than other follicles (p < 0.05). The RAC1 protein was mainly expressed in oocyte and its around GCs and stromal tissues of the prehierarchical follicles by immunohistochemistry. Further investigation revealed the RAC1 gene remarkably enhanced the mRNA and protein expression levels of FSHR (a marker of follicle selection), CCND2 (a marker of cell-cycle progression and GC differentiation), PCNA (a marker of GC proliferation), StAR and CYP11A1 (markers of GC differentiation and steroidogenesis) (p < 0.05). Furthermore, our data demonstrated siRNA interference of RAC1 significantly reduced GC proliferation (p < 0.05), while RAC1 gene overexpression enhanced GC proliferation in vitro (p < 0.05). Collectively, this study provided new evidence that the biological effects of RAC1 on GC proliferation, differentiation and steroidogenesis of chicken ovary follicles. Full article

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13 pages, 15155 KiB

Open AccessArticle

Effect of Biochar Diet Supplementation on Chicken Broilers Performance, NH₃ and Odor Emissions and Meat Consumer Acceptance

by Kajetan Kalus, Damian Konkol, Mariusz Korczyński, Jacek A. Koziel and Sebastian Opaliński

Animals 2020, 10(9), 1539; https://doi.org/10.3390/ani10091539 - 1 Sep 2020

Cited by 24 | Viewed by 7586

Abstract

The aim of this research was to evaluate the effect of biochar diet supplementation for broiler chickens on (1) ammonia and odor emissions from manure, (2) feed conversion ratio and daily weight gain, and (3) selected meat quality and sensory parameters. Beechwood biochar [...] Read more.

The aim of this research was to evaluate the effect of biochar diet supplementation for broiler chickens on (1) ammonia and odor emissions from manure, (2) feed conversion ratio and daily weight gain, and (3) selected meat quality and sensory parameters. Beechwood biochar (BC, 2 and 4%) and BC–glycerin–aluminosilicates mix (BCM, 3 and 6%) were tested as dietary additives. A total of 750 chicken broilers (Ross 308) were divided into five dietary groups with five replicates per group (n = 5, 30 birds in each replicate) and reared on a littered floor for 5 weeks. Both feed additives showed a significant reduction of ammonia emissions by up to 17%, while the reduction of odor emissions was not statistically significant. The feed conversion ratio increased by 8% for the highest concentration of the mixture. The change of the treated broilers’ average body weight ranged in the last week of the experiment from 0 to −7%, with the most negative effect for the highest dose of the mixture. Sensory analysis of the sous-vide cooked breasts showed no significant differences. Full article

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12 pages, 1840 KiB

Open AccessArticle

Avian Satellite Cell Plasticity

by Maurycy Jankowski, Paul Mozdziak, James Petitte, Magdalena Kulus and Bartosz Kempisty

Animals 2020, 10(8), 1322; https://doi.org/10.3390/ani10081322 - 31 Jul 2020

Cited by 14 | Viewed by 3155

Abstract

Adult myogenesis is dependent on a population of precursor cells, located between the sarcolemma and the basal lamina of the muscle fiber. These satellite cells, usually present in a quiescent state, become activated in response to mechanical muscle strain, differentiating and fusing to [...] Read more.

Adult myogenesis is dependent on a population of precursor cells, located between the sarcolemma and the basal lamina of the muscle fiber. These satellite cells, usually present in a quiescent state, become activated in response to mechanical muscle strain, differentiating and fusing to add new nuclei to enlarging muscles. As their myogenic lineage commitment is induced on demand, muscle satellite cells exhibit a certain amount of plasticity, possibly being able to be directed to differentiate into non-myogenic fates. In this study, myosatellite cells were isolated from chicken muscle samples, characterized in vitro and introduced into developing blastoderms. They were further investigated using fluorescence microscopy, immunohistochemistry and PCR, to determine their location in embryos after three and eighteen days. The results of the in vitro analysis confirmed that the cells obtained from the Pectoralis thoracicus are highly myogenic, based on the expression of Pax7, Myogenin, MyoD, Desmin and the myotube assay. Furthermore, the investigation of satellite cells within the embryo showed their migration to the regions of Pectoralis thoracicus, heart, liver, gizzard, proventriculus, intestine and brain. Overall, the results of the study proved the high myogenicity of chicken Pectoralis thoracicus cell isolates, as well as provided new information about their migration pathways following introduction into the blastocyst. The presence of the introduced LacZ or eGFP transgenes across the embryo, even 20 days after myosatellite cell injection, further supports the notion that satellite cells exhibit significant plasticity, potentially transdifferentiating into non-muscle lineages. Full article

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Open AccessArticle

The Effects of Feed Particle Size and Floor Type on the Growth Performance, GIT Development, and Pododermatitis in Broiler Chickens

by Amr Abd El-Wahab, Jan-Philip Kriewitz, Julia Hankel, Bussarakam Chuppava, Christine Ratert, Venja Taube, Christian Visscher and Josef Kamphues

Animals 2020, 10(8), 1256; https://doi.org/10.3390/ani10081256 - 24 Jul 2020

Cited by 14 | Viewed by 3924

Abstract

The aim of the present study was to evaluate the effects of feed particle size and flooring designs on organ traits, performance and pododermatitis in broilers. A total of 480 broilers (Ross 308) of both sexes were randomly assigned to two feeding groups [...] Read more.

The aim of the present study was to evaluate the effects of feed particle size and flooring designs on organ traits, performance and pododermatitis in broilers. A total of 480 broilers (Ross 308) of both sexes were randomly assigned to two feeding groups (finely or coarsely ground pelleted diets; with addition of 5% to 10% intact wheat in coarsely diets) and four different housing systems (litter; litter with floor heating; partially or fully slatted floor) with three subgroups each. A coarse diet increased the final gizzard and pancreas weights (p < 0.001) while decreasing the risk of Isthmus gastrici dilatation compared to a fine diet (p < 0.001). Broilers fed a coarse diet displayed an increased final body weight (p = 0.023) and led to a favourable feed conversion ratio. Final body weight was the highest (p < 0.001) for birds housed on partially or fully slatted floor. Housing birds on litter with floor heating showed the lowest pododermatitis scoring (p < 0.001). It seems to be favourable to use coarse diets for organ development, whereas slatted floors seem to foster enlargement of the Isthmus gastrici. Increasing growth performance was possible both when using coarse diets or slatted floors. Full article

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