Liquid fermentation is an efficient culture for obtaining polysaccharides from edible mushrooms. In this study, the polysaccharide content and biomass were examined by introducing microorganisms into the
Wolfiporia cocos fermentation system. Three edible mushroom co-fermentation systems were established, among which the
Wolfiporia cocos-Ganoderma
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Liquid fermentation is an efficient culture for obtaining polysaccharides from edible mushrooms. In this study, the polysaccharide content and biomass were examined by introducing microorganisms into the
Wolfiporia cocos fermentation system. Three edible mushroom co-fermentation systems were established, among which the
Wolfiporia cocos-Ganoderma lucidum co-fermentation system significantly increased the mycelial biomass of the system by 57.71% compared to
Wolfiporia cocos alone and 91.22% compared to
Ganoderma lucidum alone, and the intracellular polysaccharide content was significantly increased. Physiological activities of polysaccharides showed that mycelial polysaccharides in the
Wolfiporia cocos-Ganoderma lucidum system had stronger anti-tumor cell value-adding and anti-tumor cell migration activities compared with
Wolfiporia cocos and
Ganoderma lucidum fermentation alone. The transcriptomic study of
Wolfiporia cocos mycelium induced by exogenous substances suggested that the exogenous substances could enhance the intracellular polysaccharide content of
Wolfiporia cocos through the upregulation of the expression of α-glycosyltransferase encoded by ALG10 and the downregulation of α-glycosidases encoded by MAN1B in the glycolytic metabolism of
Wolfiporia cocos. This study provides a new direction for the transformation of polysaccharides from
Wolfiporia cocos and
Ganoderma lucidum into functional foods and new product development, and provides an experimental basis.
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